scholarly journals Staphylococcus saccharolyticus: An Overlooked Human Skin Colonizer

2020 ◽  
Vol 8 (8) ◽  
pp. 1105
Author(s):  
Charlotte M. Ahle ◽  
Kristian Stødkilde ◽  
Mastaneh Afshar ◽  
Anja Poehlein ◽  
Lesley A. Ogilvie ◽  
...  
Keyword(s):  
Human Skin ◽  
Staphylococcus Epidermidis ◽  
Amplicon Sequencing ◽  
Growth Conditions ◽  
Skin Microbiota ◽  
Healthy Human ◽  
Staphylococcal Species ◽  
Staphylococcus Capitis ◽  
Culture Independent ◽  
Slow Growing

Coagulase-negative staphylococcal species constitute an important part of the human skin microbiota. In particular, facultative anaerobic species such as Staphylococcus epidermidis and Staphylococcus capitis can be found on the skin of virtually every human being. Here, we applied a culture-independent amplicon sequencing approach to identify staphylococcal species on the skin of healthy human individuals. While S. epidermidis and S. capitis were found as primary residents of back skin, surprisingly, the third most abundant member was Staphylococcus saccharolyticus, a relatively unstudied species. A search of skin metagenomic datasets detected sequences identical to the genome of S. saccharolyticus in diverse skin sites, including the back, forehead, and elbow pit. Although described as a slow-growing anaerobic species, a re-evaluation of its growth behavior showed that S. saccharolyticus can grow under oxic conditions, and, in particular, in a CO2-rich atmosphere. We argue here that S. saccharolyticus was largely overlooked in previous culture-dependent and -independent studies, due to its requirement for fastidious growth conditions and the lack of reference genome sequences, respectively. Future studies are needed to unravel the microbiology and host-interacting properties of S. saccharolyticus and its role as a prevalent skin colonizer.

scholarly journals Draft Genome Sequences of Four Commensal Strains of Staphylococcus and Pseudomonas Isolated from Healthy Human Skin

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Xavier Janvier ◽  
Amine M. Boukerb ◽  
Marc G. J. Feuilloley ◽  
Anne Groboillot
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Fluorescens ◽  
Human Skin ◽  
Staphylococcus Epidermidis ◽  
Sequence Data ◽  
Draft Genome ◽  
Genome Sequences ◽  
Healthy Human ◽  
Content Type ◽  
Staphylococcus Capitis

ABSTRACT Staphylococcus spp. and Pseudomonas spp. are widely distributed bacteria in the environment and are found in association with animals and humans. Here, we present the draft genome sequence data of the healthy human skin commensal strains Staphylococcus aureus MFP03, Staphylococcus epidermidis MFP04, Staphylococcus capitis MFP08, and Pseudomonas fluorescens MFP05.

scholarly journals Comparison of three amplicon sequencing approaches to determine staphylococcal populations on human skin

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Charlotte Marie Ahle ◽  
Kristian Stødkilde-Jørgensen ◽  
Anja Poehlein ◽  
Wolfgang R. Streit ◽  
Jennifer Hüpeden ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Diseases ◽  
Cost Effective ◽  
Amplicon Sequencing ◽  
Species Level ◽  
Skin Microbiome ◽  
Staphylococcal Species ◽  
Culture Independent ◽  
Skin Samples ◽  
Mock Communities

Abstract Background Staphylococci are important members of the human skin microbiome. Many staphylococcal species and strains are commensals of the healthy skin microbiota, while few play essential roles in skin diseases such as atopic dermatitis. To study the involvement of staphylococci in health and disease, it is essential to determine staphylococcal populations in skin samples beyond the genus and species level. Culture-independent approaches such as amplicon next-generation sequencing (NGS) are time- and cost-effective options. However, their suitability depends on the power of resolution. Results Here we compare three amplicon NGS schemes that rely on different targets within the genes tuf and rpsK, designated tuf1, tuf2 and rpsK schemes. The schemes were tested on mock communities and on human skin samples. To obtain skin samples and build mock communities, skin swab samples of healthy volunteers were taken. In total, 254 staphylococcal strains were isolated and identified to the species level by MALDI-TOF mass spectrometry. A subset of ten strains belonging to different staphylococcal species were genome-sequenced. Two mock communities with nine and eighteen strains, respectively, as well as eight randomly selected skin samples were analysed with the three amplicon NGS methods. Our results imply that all three methods are suitable for species-level determination of staphylococcal populations. However, the novel tuf2-NGS scheme was superior in resolution power. It unambiguously allowed identification of Staphylococcus saccharolyticus and distinguish phylogenetically distinct clusters of Staphylococcus epidermidis. Conclusions Powerful amplicon NGS approaches for the detection and relative quantification of staphylococci in human samples exist that can resolve populations to the species and, to some extent, to the subspecies level. Our study highlights strengths, weaknesses and pitfalls of three currently available amplicon NGS approaches to determine staphylococcal populations. Applied to the analysis of healthy and diseased skin, these approaches can be useful to attribute host-beneficial and -detrimental roles to skin-resident staphylococcal species and subspecies.

Detection of potentially novel bacterial components of the human skin microbiota using culture-independent molecular profiling

2005 ◽  
Vol 54 (12) ◽  
pp. 1231-1238 ◽  
Author(s):  
Itaru Dekio ◽  
Hidenori Hayashi ◽  
Mitsuo Sakamoto ◽  
Maki Kitahara ◽  
Takeji Nishikawa ◽  
...  
Keyword(s):  
Human Skin ◽  
Molecular Profiling ◽  
Skin Microbiota ◽  
Culture Independent

scholarly journals Detection of Staphylococcal Species Diversity within Human Skin Microbiome Using a Simple PCR-SSCP Technique

2018 ◽  
Vol 26 (8) ◽  
pp. 1-6
Author(s):  
Lubab Aqeel ◽  
Rabab Omran
Keyword(s):  
Species Diversity ◽  
Human Skin ◽  
Genomic Dna ◽  
Skin Microbiome ◽  
Healthy Individuals ◽  
Bacterial Strains ◽  
Healthy Human ◽  
Single Strand Conformational Polymorphism ◽  
Staphylococcal Species ◽  
Pcr Products

Objective. The aim of this study was to detect the diversity of Staphylococcus species of a healthy human skin using a simple techniquevPCR-SSCP. Methods: Blood samples, saliva, and skin swaps samples were collected from 50 persons from Hilla City  - Iraq. The genomic DNA was extracted from these samples using the Bacteria Genomic DNA Kit. The concentrations and purity of DNA extract estimated by NanoDrop spectrophotometer. Polymerase chain reaction – single-strand conformational polymorphism (PCR–SSCP) technique was performed to detect the diversity between Staphylococcal species in the human skin microbiome using a specific primer of the 16SrRNA gene. Results: The PCR results, indicated that the Staphylococcal species were found within the ski community, but it's not infected blood and mouth of test healthy individuals. SSCP-heteroduplex patterns of PCR products appeared the presence Staphylococcal species diversity within skin microbiome of test healthy individuals. Conclusion: In spite of the PCR-SSCP, heteroduplex method was simple and cheap and appeared the diversity betweenvStaphylococcalspeciesvin the human skin microbiome, but it's not diagnosed the bacterial strains. So these results required to confirm by DNAvsequencingvtechnique.

Formation and Properties of Persister Cells of Staphylococcus capitis and Staphylococcus epidermidis, Bacteria Inhabiting Human Skin

Microbiology ◽  
2020 ◽  
Vol 89 (4) ◽  
pp. 425-434
Author(s):  
Yu. A. Nikolaev ◽  
T. A. Pankratov ◽  
A. V. Gannesen ◽  
T. V. Kolganova ◽  
N. E. Suzina ◽  
...  
Keyword(s):  
Human Skin ◽  
Staphylococcus Epidermidis ◽  
Persister Cells ◽  
Staphylococcus Capitis

scholarly journals Deleterious Effects of an Air Pollutant (NO2) on a Selection of Commensal Skin Bacterial Strains, Potential Contributor to Dysbiosis?

2020 ◽  
Vol 11 ◽  
Author(s):  
Xavier Janvier ◽  
Stéphane Alexandre ◽  
Amine M. Boukerb ◽  
Djouhar Souak ◽  
Olivier Maillot ◽  
...  
Keyword(s):  
Air Pollution ◽  
Human Skin ◽  
Nitrosative Stress ◽  
Bacterial Species ◽  
Air Pollutant ◽  
Skin Microbiota ◽  
Staphylococcus Capitis ◽  
Short Period ◽  
The Impact ◽  
Gas Exposure

The skin constitutes with its microbiota the first line of body defense against exogenous stress including air pollution. Especially in urban or sub-urban areas, it is continuously exposed to many environmental pollutants including gaseous nitrogen dioxide (gNO2). Nowadays, it is well established that air pollution has major effects on the human skin, inducing various diseases often associated with microbial dysbiosis. However, very few is known about the impact of pollutants on skin microbiota. In this study, a new approach was adopted, by considering the alteration of the cutaneous microbiota by air pollutants as an indirect action of the harmful molecules on the skin. The effects of gNO2 on this bacterial skin microbiota was investigated using a device developed to mimic the real-life contact of the gNO2 with bacteria on the surface of the skin. Five strains of human skin commensal bacteria were considered, namely Staphylococcus aureus MFP03, Staphylococcus epidermidis MFP04, Staphylococcus capitis MFP08, Pseudomonas fluorescens MFP05, and Corynebacterium tuberculostearicum CIP102622. Bacteria were exposed to high concentration of gNO2 (10 or 80 ppm) over a short period of 2 h inside the gas exposure device. The physiological, morphological, and molecular responses of the bacteria after the gas exposure were assessed and compared between the different strains and the two gNO2 concentrations. A highly significant deleterious effect of gNO2 was highlighted, particularly for S. capitis MFP08 and C. tuberculostearicum CIP102622, while S. aureus MFP03 seems to be the less sensitive strain. It appeared that the impact of this nitrosative stress differs according to the bacterial species and the gNO2 concentration. Thus the exposition to gNO2 as an air pollutant could contribute to dysbiosis, which would affect skin homeostasis. The response of the microbiota to the nitrosative stress could be involved in some pathologies such as atopic dermatitis.

scholarly journals Draft Genome Sequence of a New Staphylococcal Species Isolated from Human Skin

2020 ◽  
Vol 9 (6) ◽  
Author(s):  
Kristian Stødkilde ◽  
Anja Poehlein ◽  
Holger Brüggemann
Keyword(s):  
Human Skin ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Draft Genome ◽  
Average Nucleotide Identity ◽  
Healthy Human ◽  
Separate Species ◽  
Content Type ◽  
Staphylococcal Species ◽  
Staphylococcus Haemolyticus

A hemolytic staphylococcal strain, Staphylococcus sp. strain 170179, was isolated from healthy human skin. Genome sequencing and comparison of strain 170179 to other staphylococci revealed a relatedness to Staphylococcus haemolyticus with an average nucleotide identity of 87.5%, indicating that Staphylococcus sp. 170179 belongs to a separate species.

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