scholarly journals Batch and Continuous Lactic Acid Fermentation Based on A Multi-Substrate Approach

2020 ◽  
Vol 8 (7) ◽  
pp. 1084
Author(s):  
Agata Olszewska-Widdrat ◽  
Maria Alexandri ◽  
José Pablo López-Gómez ◽  
Roland Schneider ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
Bacillus Coagulans ◽  
Batch Experiments ◽  
Standard Medium ◽  
Acid Fermentation ◽  
Conversion Yield ◽  
Platform Chemicals ◽  
A Value ◽  
Acid Whey ◽  
Sugar Conversion

The utilisation of waste materials and industrial residues became a priority within the bioeconomy concept and the production of biobased chemicals. The aim of this study was to evaluate the feasibility to continuously produce L-lactic acid from different renewable substrates, in a multi-substrate strategy mode. Based on batch experiments observations, Bacillus coagulans A534 strain was able to continuously metabolise acid whey, sugar beet molasses, sugar bread, alfalfa press green juice and tapioca starch. Additionally, reference experiments showed its behaviour in standard medium. Continuous fermentations indicated that the highest productivity was achieved when molasses was employed with a value of 10.34 g·L−1·h−1, while the lactic acid to sugar conversion yield was 0.86 g·g−1. This study demonstrated that LA can be efficiently produced in continuous mode regardless the substrate, which is a huge advantage in comparison to other platform chemicals.

scholarly journals Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618T and Subsequent Lactic Acid Fermentation

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2626 ◽  
Author(s):  
Yuejiao Xie ◽  
Qing Hu ◽  
Guodong Feng ◽  
Xu Jiang ◽  
Jinlong Hu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
High Titer ◽  
Bacillus Coagulans ◽  
High Yield ◽  
Acid Fermentation ◽  
Biochemical Production ◽  
Water Rinsing ◽  
Simultaneous Saccharification

Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618T, a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618T into the NH3/H2O2-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 °C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH3/H2O2-pretreated corn stover with or without K. huakuii LAM0618T-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH3/H2O2-pretreated K. huakuii LAM0618T-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources.

Study on Characteristics of Lactobacillus rhamnosus on Lactic Acid Fermentation with Different Substrates

2014 ◽  
Vol 665 ◽  
pp. 388-392
Author(s):  
Ning Li ◽  
Yu Cai Lü ◽  
Da Chun Gong
Keyword(s):  
Lactic Acid ◽  
Conversion Rate ◽  
Lactobacillus Rhamnosus ◽  
Reducing Sugar ◽  
Lactic Acid Fermentation ◽  
Sugar Concentration ◽  
Wet Oxidation ◽  
Acid Fermentation ◽  
Substrate Conversion ◽  
Sugar Conversion

In order to investigate characteristics of Lactobacillus rhamnosus on lactic acid fermentation using different substrate, in this study, saccharification liquid obtained during wet oxidation blasting of straw were used as substrate, conversion reducing sugar to lactate by Lactobacillus rhamnosus was studied and compared with fermentation using glucose as substrate, The results indicated that Lactobacillus rhamnosus could utilize reducing sugar in saccharification liquid obtained during wet oxidation blasting of straw effectively. The highest efficiency of reducing sugar conversion (92.45%) appeared when concentration of reducing sugar as substrate was 20mg/mL; conversion rate of reducing sugar decreased as increasing of sugar concentration. Fermentation of saccharification liquid obtained during wet oxidation blasting of straw to lactic acid by Lactobacillus rhamnosus had optimal effects in the first 48h of incubation.

Ultrafiltration flux of acid whey obtained by lactic acid fermentation

2012 ◽  
Vol 22 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Gerd Konrad ◽  
Thomas Kleinschmidt ◽  
Wolfgang Faber
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation ◽  
Acid Whey

Conversion of hydrophilic constituents from Mercurialis perennis L. by lactic acid fermentation

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
P Lorenz ◽  
S Duckstein ◽  
J Bertrams ◽  
U Meyer ◽  
F Stintzing
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation
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