scholarly journals SARS-CoV-2 Virologic and Immunologic Correlates in Patients with Olfactory and Taste Disorders

2020 ◽  
Vol 8 (7) ◽  
pp. 1052
Author(s):  
Marco Benazzo ◽  
Irene Cassaniti ◽  
Eugenia Maiorano ◽  
Anna Calastri ◽  
Federica Novazzi ◽  
...  
Keyword(s):  
Median Time ◽  
Crucial Role ◽  
Great Majority ◽  
Molecular Assays ◽  
Nasal Swabs ◽  
Taste Disorders ◽  
Over Time

The main object of the study was to investigate the SARS-CoV-2 molecular and serological pattern in patients with mild symptoms including anosmia and ageusia. A cohort of 69 patients with olfactory and taste disorders (OTDs) were enrolled and prospectively monitored. Serological and molecular assays for the characterization of SARS-CoV-2 IgG and SARS-CoV-2 RNA, respectively, were performed at the time of enrolment and after 7 and 14 days. Patients were stratified according to the symptoms’ onset. A total of 52 patients (75.4%) were diagnosed as COVID-19 positive being SARS-CoV-2 RNA and/or SARS-CoV-2 IgG positive. The remaining 17 (24.6%) were negative for COVID-19 and excluded from the analysis. We reported that only 34 out of 52 patients (65.4%) were positive for SARS-CoV-2 RNA. Moreover, the median time from onset of symptoms and enrolment was significantly higher in those patients with negative SARS-CoV-2 RNA in nasal swabs, suggesting that symptoms might last longer than SARS-CoV-2 replication. The great majority of patients (80%) developed SARS-CoV-2 IgG at three weeks after symptoms’ onset while the detectability of SARS-CoV-2 RNA dramatically decreased over time, suggesting the crucial role of combination of molecular and serological assays for the diagnosis of COVID-19 in those patients reporting mild symptoms.

scholarly journals Effects of Dysbiosis and Dietary Manipulation on the Digestive Microbiota of a Detritivorous Arthropod

2021 ◽  
Vol 9 (1) ◽  
pp. 148
Author(s):  
Marius Bredon ◽  
Elisabeth Depuydt ◽  
Lucas Brisson ◽  
Laurent Moulin ◽  
Ciriac Charles ◽  
...  
Keyword(s):  
Crucial Role ◽  
Structure And Function ◽  
Ecological Interactions ◽  
Dietary Manipulation ◽  
Biotic Factors ◽  
Abiotic And Biotic Factors ◽  
And Function ◽  
Multicellular Organisms ◽  
Over Time

The crucial role of microbes in the evolution, development, health, and ecological interactions of multicellular organisms is now widely recognized in the holobiont concept. However, the structure and stability of microbiota are highly dependent on abiotic and biotic factors, especially in the gut, which can be colonized by transient bacteria depending on the host’s diet. We studied these impacts by manipulating the digestive microbiota of the detritivore Armadillidium vulgare and analyzing the consequences on its structure and function. Hosts were exposed to initial starvation and then were fed diets that varied the different components of lignocellulose. A total of 72 digestive microbiota were analyzed according to the type of the diet (standard or enriched in cellulose, lignin, or hemicellulose) and the period following dysbiosis. The results showed that microbiota from the hepatopancreas were very stable and resilient, while the most diverse and labile over time were found in the hindgut. Dysbiosis and selective diets may have affected the host fitness by altering the structure of the microbiota and its predicted functions. Overall, these modifications can therefore have effects not only on the holobiont, but also on the “eco-holobiont” conceptualization of macroorganisms.

scholarly journals Documentation at the Ethnographic Museum in Zagreb

2019 ◽  
pp. 71-86
Author(s):  
Aleksandra Vlatković
Keyword(s):  
Crucial Role ◽  
Technological Development ◽  
International Standards ◽  
Management Activity ◽  
Concise Overview ◽  
The People ◽  
Over Time

The paper provides a concise overview of documentation management activity per- formed at the Ethnographic Museum in Zagreb during the period ranging from 1919 to this day. It strives to highlight the importance, as well as the changes and the expan- sion of the role of documentation management in museums over time. The continuity of documentation management at the Ethnographic Museum has been pointed out over the first 100 years of its activity and the work of the people who have played a crucial role in the formation and the documentation management at the Museum has been concisely presented. The conclusion presents the current challenges and efforts to harmonise the documentation management tradition with the changes in laws, technological development and international standards

Loneliness over time: The crucial role of social anxiety.

2016 ◽  
Vol 125 (5) ◽  
pp. 620-630 ◽  
Author(s):  
Michelle H. Lim ◽  
Thomas L. Rodebaugh ◽  
Michael J. Zyphur ◽  
John F. M. Gleeson
Keyword(s):  
Social Anxiety ◽  
Crucial Role ◽  
Over Time

scholarly journals Optical properties and carrier dynamics in Co-doped ZnO nanorods

2021 ◽  
Vol 3 (1) ◽  
pp. 214-222
Author(s):  
Aswathi K. Sivan ◽  
Alejandro Galán-González ◽  
Lorenzo Di Mario ◽  
Nicolas Tappy ◽  
Javier Hernández-Ferrer ◽  
...  
Keyword(s):  
Optical Properties ◽  
Crucial Role ◽  
Surface Defects ◽  
Zno Nanorods ◽  
Carrier Dynamics ◽  
Photoelectrochemical Characterization ◽  
Doped Zno ◽  
Co Doped

Optoelectronic and photoelectrochemical characterization of Co-doped ZnO nanorods revealing the crucial role of surface defects for enhanced photoresponse.

The ability of the NiSOD binding loop to chelate zinc(ii): the role of the terminal amino group in the enzymatic functions

2019 ◽  
Vol 48 (18) ◽  
pp. 6217-6227 ◽  
Author(s):  
Gizella Csire ◽  
András Kolozsi ◽  
Tamás Gajda ◽  
Giuseppe Pappalardo ◽  
Katalin Várnagy ◽  
...  
Keyword(s):  
Crucial Role ◽  
Spectroscopic Characterization ◽  
Amino Group ◽  
Amino Groups ◽  
Enzymatic Function ◽  
Terminal Amino ◽  
Binding Loop

Equilibrium and spectroscopic characterization of zinc(ii) complexes with NiSOD related peptides highlights the crucial role of terminal amino groups in the enzymatic function.

scholarly journals Frontispiece: Growth and Atomic‐Scale Characterization of Ultrathin Silica and Germania Films: The Crucial Role of the Metal Support

2021 ◽  
Vol 27 (6) ◽  
Author(s):  
Adrián Leandro Lewandowski ◽  
Sergio Tosoni ◽  
Leonard Gura ◽  
Zechao Yang ◽  
Alexander Fuhrich ◽  
...  
Keyword(s):  
Crucial Role ◽  
Atomic Scale ◽  
Metal Support ◽  
Scale Characterization

scholarly journals Growth and Atomic‐Scale Characterization of Ultrathin Silica and Germania Films: The Crucial Role of the Metal Support

2020 ◽  
Author(s):  
Adrián Leandro Lewandowski ◽  
Sergio Tosoni ◽  
Leonard Gura ◽  
Zechao Yang ◽  
Alexander Fuhrich ◽  
...  
Keyword(s):  
Crucial Role ◽  
Atomic Scale ◽  
Metal Support ◽  
Scale Characterization

scholarly journals Characterization of Lysine Acetyltransferase Activity of Recombinant Human ARD1/NAA10

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 588 ◽  
Author(s):  
Tam Thuy Lu Vo ◽  
Ji-Hyeon Park ◽  
Eun Ji Lee ◽  
Yen Thi Kim Nguyen ◽  
Byung Woo Han ◽  
...  
Keyword(s):  
Size Exclusion ◽  
Lysine Acetylation ◽  
Monomeric Form ◽  
Experimental Conditions ◽  
Lysine Residues ◽  
Lysine Acetyltransferase ◽  
Over Time

Arrest defective 1 (ARD1), also known as N(alpha)-acetyltransferase 10 (NAA10) was originally identified as an N-terminal acetyltransferase (NAT) that catalyzes the acetylation of N-termini of newly synthesized peptides. After that, mammalian ARD1/NAA10 expanded its’ role to lysine acetyltransferase (KAT) that post-translationally acetylates internal lysine residues of proteins. ARD1/NAA10 is the only enzyme with both NAT and KAT activities. However, recent studies on the role of human ARD1/NAA10 (hARD1/NAA10) in lysine acetylation are contradictory, as crystal structure and in vitro acetylation assay results revealed the lack of KAT activity. Thus, the role of hARD1/NAA10 in lysine acetylation is still debating. Here, we found a clue that possibly explains these complicated and controversial results on KAT activity of hARD1/NAA10. Recombinant hARD1/NAA10 exhibited KAT activity, which disappeared soon in vitro. Size-exclusion analysis revealed that most recombinant hARD1/NAA10 formed oligomers over time, resulting in the loss of KAT activity. While oligomeric recombinant hARD1/NAA10 lost its ability for lysine acetylation, its monomeric form clearly exhibited lysine acetylation activity in vitro. We also characterized the KAT activity of hARD1/NAA10 that was influenced by several experimental conditions, including concentration of reactants and reaction time. Taken together, our study proves that recombinant hARD1/NAA10 exhibits KAT activity in vitro but only under accurate conditions, including reactant concentrations and reaction duration.

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