scholarly journals Comparative Genomics of Beggiatoa leptomitoformis Strains D-401 and D-402T with Contrasting Physiology But Extremely High Level of Genomic Identity

2020 ◽  
Vol 8 (6) ◽  
pp. 928
Author(s):  
Tatyana S. Rudenko ◽  
Sergey V. Tarlachkov ◽  
Nikolay D. Shatskiy ◽  
Margarita Yu. Grabovich
Keyword(s):  
Comparative Genomics ◽  
Sulfur Compounds ◽  
Single Letter ◽  
Reduced Sulfur Compounds ◽  
Gene Coding ◽  
Genes Encoding ◽  
Oxidation Of Hydrogen ◽  
Sulfur Globules ◽  
Reduced Sulfur ◽  
High Level

Representatives of filamentous colorless sulfur-oxidizing bacteria often dominate in sulfide biotopes, preventing the diffusion of toxic sulfide into the water column. One of the most intriguing groups is a recently described Beggiatoa leptomitoformis including strains D-401 and D-402T. Both strains have identical genes encoding enzymes which are involved in the oxidation of hydrogen sulfide and thiosulfate. Surprisingly, the B. leptomitoformis strain D-401 is not capable to grow lithotrophically in the presence of reduced sulfur compounds and to accumulate elemental sulfur inside the cells, in contrast to the D-402T strain. In general, genomes of D-401 and D-402T have an extremely high level of identity and only differ in 1 single-letter substitution, 4 single-letter indels, and 16 long inserts. Among long inserts, 14 are transposons. It was shown that in the D-401 strain, a gene coding for a sulfur globule protein was disrupted by one of the mentioned transposons. Based on comparative genomics, RT-qPCR, and HPLC-MS/MS, we can conclude that this gene plays a crucial role in the formation of the sulfur globules inside the cells, and the disruption of its function prevents lithotrophic growth of B. leptomitoformis in the presence of reduced sulfur compounds.

scholarly journals Regulation of dsr genes encoding proteins responsible for the oxidation of stored sulfur in Allochromatium vinosum

Microbiology ◽  
2010 ◽  
Vol 156 (3) ◽  
pp. 764-773 ◽  
Author(s):  
Frauke Grimm ◽  
Nadine Dobler ◽  
Christiane Dahl
Keyword(s):  
Gene Expression ◽  
Regulatory Function ◽  
Dependent Manner ◽  
Reduced Sulfur Compounds ◽  
Allochromatium Vinosum ◽  
Sulfate Production ◽  
Genes Encoding ◽  
Sulfur Globules ◽  
Reduced Sulfur ◽  
Chemolithoautotrophic Bacteria

Sulfur globules are formed as obligatory intermediates during the oxidation of reduced sulfur compounds in many environmentally important photo- and chemolithoautotrophic bacteria. It is well established that the so-called Dsr proteins are essential for the oxidation of zero-valent sulfur accumulated in the globules; however, hardly anything is known about the regulation of dsr gene expression. Here, we present a closer look at the regulation of the dsr genes in the phototrophic sulfur bacterium Allochromatium vinosum. The dsr genes are expressed in a reduced sulfur compound-dependent manner and neither sulfite, the product of the reverse-acting dissimilatory sulfite reductase DsrAB, nor the alternative electron donor malate inhibit the gene expression. Moreover, we show the oxidation of sulfur to sulfite to be the rate-limiting step in the oxidation of sulfur to sulfate as sulfate production starts concomitantly with the upregulation of the expression of the dsr genes. Real-time RT-PCR experiments suggest that the genes dsrC and dsrS are additionally expressed from secondary internal promoters, pointing to a special function of the encoded proteins. Earlier structural analyses indicated the presence of a helix–turn–helix (HTH)-like motif in DsrC. We therefore assessed the DNA-binding capability of the protein and provide evidence for a possible regulatory function of DsrC.

Acute and subchronic toxicity studies of rats exposed to vapors of methyl mercaptan and other reduced‐sulfur compounds

1981 ◽  
Vol 8 (1-2) ◽  
pp. 71-88 ◽  
Author(s):  
Martin F. Tansy ◽  
Frank M. Kendall ◽  
John Fantasia ◽  
Wendell E. Landin ◽  
Richard Oberly ◽  
...  
Keyword(s):  
Sulfur Compounds ◽  
Methyl Mercaptan ◽  
Reduced Sulfur Compounds ◽  
Subchronic Toxicity ◽  
Toxicity Studies ◽  
Reduced Sulfur

Methanesulfonic Acid and Sulfuric Acid Aerosol Formed through Oxidation of Reduced Sulfur Compounds in a Humid Environment

2021 ◽  
pp. 118504
Author(s):  
Paul Van Rooy ◽  
Ryan Drover ◽  
Tanner Cress ◽  
Cara Michael ◽  
Kathleen L. Purvis-Roberts ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Methanesulfonic Acid ◽  
Sulfur Compounds ◽  
Reduced Sulfur Compounds ◽  
Humid Environment ◽  
Reduced Sulfur

scholarly journals Use of reduced sulfur compounds by Beggiatoa sp.

1981 ◽  
Vol 147 (1) ◽  
pp. 140-154 ◽  
Author(s):  
D C Nelson ◽  
R W Castenholz
Keyword(s):  
Sulfur Compounds ◽  
Reduced Sulfur Compounds ◽  
Reduced Sulfur

scholarly journals The production and utilization of intermediary elemental sulfur during the oxidation of reduced sulfur compounds by Thiobacillus ferrooxidans

1988 ◽  
Vol 150 (6) ◽  
pp. 574-579 ◽  
Author(s):  
W. Hazeu ◽  
W. H. Batenburg-van der Vegte ◽  
P. Bos ◽  
R. K. van der Pas ◽  
J. G. Kuenen
Keyword(s):  
Elemental Sulfur ◽  
Thiobacillus Ferrooxidans ◽  
Sulfur Compounds ◽  
Reduced Sulfur Compounds ◽  
Reduced Sulfur

Chemiluminescent detection of reduced sulfur compounds with ozone

1983 ◽  
Vol 55 (1) ◽  
pp. 135-138 ◽  
Author(s):  
Thomas J. Kelly ◽  
Jeffrey S. Gaffney ◽  
Mary F. Phillips ◽  
Roger L. Tanner
Keyword(s):  
Sulfur Compounds ◽  
Reduced Sulfur Compounds ◽  
Chemiluminescent Detection ◽  
Reduced Sulfur
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