scholarly journals Molecular Characterization and Pathogenicity of Staphylococcus aureus Isolated from Benin-City, Nigeria

2020 ◽  
Vol 8 (6) ◽  
pp. 912 ◽  
Author(s):  
Osahon Obasuyi ◽  
JoAnn McClure ◽  
Francis E. Oronsaye ◽  
John O. Akerele ◽  
John Conly ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Epidemiology ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Developed Countries ◽  
Small Degree ◽  
Local Conditions ◽  
Benin City

While numerous studies examine the epidemiology and molecular characterization of Staphylococcus aureus in most developed countries, the detailed molecular characterization and molecular epidemiology of S. aureus strains and clones in Africa is lacking. We determined the molecular epidemiology and virulence of 81 non-duplicate isolates of S. aureus from Benin-City, Nigeria, collected during January–July 2016, and compared with global strains. Forty-seven isolates (58.0%) were found to be methicillin-sensitive Staphylococcus aureus (MSSA), while 34 (42.0%) were methicillin-resistant Staphylococcus aureus (MRSA). ST152-MSSA (24.7%) and ST7-MRSA-V (19.8%) were the dominant groups identified, which were not genetically related to global predominant strains, but rather exhibited regional dominance. An interesting finding of the study was the presence of highly related strains in the region, which differed primarily in their methicillin resistance gene carriage, staphylococcal cassette chromosome mec (SCCmec), with 99.4–99.7% relatedness between the genomes of the strains within the MRSA–MSSA pairs. This suggests that the strains within a pair are experiencing gain or loss of SCCmec within local conditions, with evolution continuing to diversify the strains to a small degree. This study represents the most comprehensive genetic and virulence study of S. aureus in Nigeria.

scholarly journals Molecular characterization of the first community-acquired methicillin-resistant Staphylococcus aureus strains from Central Iran

2013 ◽  
Vol 17 (11) ◽  
pp. e949-e954 ◽  
Author(s):  
Alireza Japoni-Nejad ◽  
Mohsen Rezazadeh ◽  
Hamid Kazemian ◽  
Nasimeh Fardmousavi ◽  
Alex van Belkum ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Central Iran ◽  
Methicillin Resistant

scholarly journals Study of Antibiotics Sensitivity Pattern And Molecular Characterization of Staphylococcus Aureus Isolated From Human And Animal Pyogenic Cases

2021 ◽  
Author(s):  
Jayshree Singh ◽  
Amit Kumar ◽  
Sharad K. Yadav ◽  
Ritika Yadav ◽  
Vinod K. Singh
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Drug Sensitivity ◽  
Methicillin Resistance ◽  
Phenotypic Characterization ◽  
Resistance Pattern ◽  
Sensitivity Pattern ◽  
Drug Resistance Pattern ◽  
Animal Isolates

Abstract Staphylococcus aureus has been described as the most common cause of human and animal diseases and has emerged as superbug due to multidrug resistance. Considering these, a total of 175 samples were collected from pyogenic cases of humans (75) and animals (100), to establish the drug resistance pattern and also for molecular characterization of human and animal isolates. Thermonuclease (nuc) gene amplification was used to confirm all presumptive S. aureus isolates and then antibiotic sensitivity and slide coagulase tests were used for phenotypic characterization of isolates. Following that, all of the isolates were subjected to PCR amplification to detect the existence of the methicillin resistance (mecA) and coagulase (coa) genes. Lastly, typing was done by using the Randomly Amplified Polymorphic DNA-PCR. The overall prevalence of S. aureus in human and animal samples was found to be 39.4%. Drug sensitivity revealed the highest resistance against the β-lactam antibiotics such as ampicillin (94.8%) and penicillin (90.6%), followed by cephalosporin (cefixime-67.7%) and quinolone (ciprofloxacin-52.1%) group of drugs. The drug sensitivity was the highest against antibiotics like chloramphenicol (95%) followed by gentamicin (90%). Among the 69 S. aureus isolates, the overall presence of MRSA was 40.5% (27.5% and 50% in human and animal isolates respectively). Total 33 isolates exhibited coa genes amplification of more than one amplicons and variable in size of 250, 450, 800, and 1100 bp. The RAPD typing revealed amplification of 5 and 6 different band patterns in humans and animals, respectively, with two common patterns suggesting a common phylogenetic profile.

Molecular characterization of community‐associated methicillin‐resistant Staphylococcus aureus from pet dogs

2019 ◽  
Vol 67 (3) ◽  
pp. 222-230
Author(s):  
Gemma C. Ma ◽  
Kate A. Worthing ◽  
Thomas Gottlieb ◽  
Michael P. Ward ◽  
Jacqueline M. Norris
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Pet Dogs
Sign in / Sign up

Export Citation Format

Share Document