scholarly journals Light Regulation of Two New Manganese Peroxidase-Encoding Genes in Trametes polyzona KU-RNW027

2020 ◽  
Vol 8 (6) ◽  
pp. 852
Author(s):  
Piyangkun Lueangjaroenkit ◽  
Emi Kunitake ◽  
Makiko Sakka ◽  
Tetsuya Kimura ◽  
Churapa Teerapatsakul ◽  
...  
Keyword(s):  
Blue Light ◽  
Enzyme Production ◽  
Molecular Mechanisms ◽  
Light Regulation ◽  
Ligninolytic Enzyme ◽  
Regulatory Elements ◽  
White Rot ◽  
White Rot Fungus ◽  
Gene Expressions ◽  
Encoding Genes

To better understand the light regulation of ligninolytic systems in Trametes polyzona KU-RNW027, ligninolytic enzymes-encoding genes were identified and analyzed to determine their transcriptional regulatory elements. Elements of light regulation were investigated in submerged culture. Three ligninolytic enzyme-encoding genes, mnp1, mnp2, and lac1, were found. Cloning of the genes encoding MnP1 and MnP2 revealed distinct deduced amino acid sequences with 90% and 86% similarity to MnPs in Lenzites gibbosa, respectively. These were classified as new members of short-type hybrid MnPs in subfamily A.2 class II fungal secretion heme peroxidase. A light responsive element (LRE), composed of a 5′-CCRCCC-3′ motif in both mnp promoters, is reported. Light enhanced MnP activity 1.5 times but not laccase activity. The mnp gene expressions under light condition increased 6.5- and 3.8-fold, respectively. Regulation of laccase gene expression by light was inconsistent with the absence of LREs in their promoter. Blue light did not affect gene expressions but impacted their stability. Reductions of MnP and laccase production under blue light were observed. The details of the molecular mechanisms underlying enzyme production in this white-rot fungus provide useful knowledge for wood degradation relative to illumination condition. These novel observations demonstrate the potential of enhancing ligninolytic enzyme production by this fungus for applications with an eco-friendly approach to bioremediation.

scholarly journals White-rot fungus: an updated review

2021 ◽  
pp. 202-217
Author(s):  
Jaspreet Kaur ◽  
Amar Pal Singh ◽  
Ajeet Pal Singh ◽  
Rajinderpal Kaur
Keyword(s):  
Stainless Steel ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Mold ◽  
White Rot Fungus ◽  
Impact Factors ◽  
Production Processes ◽  
Polyamide Fiber

The White Fungus, which causes white rot on tree trunks, belongs to the basidiomycetes. Research into the microbiology of White-rot fungi has focused on engineering processes related to factors such as cell growth and enzyme production processes, and to smaller, i.e., molecular biology. Many studies have been conducted to select issues with high or specific biodegradation performance in a variety of ways. Production inhibitors have been used to improve enzyme production. Investigators are investigating different carriers (Stainless Steel net, polyamide fiber net, fiberglass net and polyurethane foam) to impair P.chrysosporium ligninolytic enzyme production. In this review, Pathophysiology, Microbiology, impact factors, treatments and alternative uses show white mold formation in biotransformation. The white fungus is being investigated to produce biotechnology for the reduction of a broad spectrum, a natural pollutant based on lignin-deficient enzymes. This in particular covers the destruction of many wastes and environmental pollution, including wastewater, pesticides, toxic natural pollutants, chlorinated hydrocarbons, etc. It will be updated.

scholarly journals Characterization of ligninolytic enzyme production in white-rot wild fungal strains suitable for kraft pulp bleaching

3 Biotech ◽  
2017 ◽  
Vol 7 (5) ◽  
Author(s):  
Rosa María Damián-Robles ◽  
Agustín Jaime Castro-Montoya ◽  
Jaime Saucedo-Luna ◽  
Ma. Soledad Vázquez-Garcidueñas ◽  
Marina Arredondo-Santoyo ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Kraft Pulp ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Pulp Bleaching ◽  
Fungal Strains ◽  
Kraft Pulp Bleaching

scholarly journals Seeing the Light: The Roles of Red- and Blue-Light Sensing in Plant Microbes

2018 ◽  
Vol 56 (1) ◽  
pp. 41-66 ◽  
Author(s):  
Gwyn A. Beattie ◽  
Bridget M. Hatfield ◽  
Haili Dong ◽  
Regina S. McGrane
Keyword(s):  
Blue Light ◽  
Molecular Mechanisms ◽  
Red Light ◽  
Light Availability ◽  
Photosynthetic Apparatus ◽  
Light Regulation ◽  
Environmental Signals ◽  
Light Sensing ◽  
Fungal Phytopathogens ◽  
Bacteria And Fungi

Plants collect, concentrate, and conduct light throughout their tissues, thus enhancing light availability to their resident microbes. This review explores the role of photosensing in the biology of plant-associated bacteria and fungi, including the molecular mechanisms of red-light sensing by phytochromes and blue-light sensing by LOV (light-oxygen-voltage) domain proteins in these microbes. Bacteriophytochromes function as major drivers of the bacterial transcriptome and mediate light-regulated suppression of virulence, motility, and conjugation in some phytopathogens and light-regulated induction of the photosynthetic apparatus in a stem-nodulating symbiont. Bacterial LOV proteins also influence light-mediated changes in both symbiotic and pathogenic phenotypes. Although red-light sensing by fungal phytopathogens is poorly understood, fungal LOV proteins contribute to blue-light regulation of traits, including asexual development and virulence. Collectively, these studies highlight that plant microbes have evolved to exploit light cues and that light sensing is often coupled with sensing other environmental signals.

Ligninolytic enzyme ability and potential biotechnology applications of the white-rot fungus Grammothele subargentea LPSC no. 436 strain

2008 ◽  
Vol 43 (4) ◽  
pp. 368-375 ◽  
Author(s):  
Mario C.N. Saparrat ◽  
Paulina Mocchiutti ◽  
Constanza S. Liggieri ◽  
Mónica B. Aulicino ◽  
Néstor O. Caffini ◽  
...  
Keyword(s):  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Genome-Wide Survey of Invertase Encoding Genes and Functional Characterization of an Extracellular Fungal Pathogen-Responsive Invertase in Glycine max

2018 ◽  
Vol 19 (8) ◽  
pp. 2395 ◽  
Author(s):  
Tao Su ◽  
Mei Han ◽  
Jie Min ◽  
Peixian Chen ◽  
Yuxin Mao ◽  
...  
Keyword(s):  
Glycine Max ◽  
Molecular Mechanisms ◽  
Soluble Sugar ◽  
Functional Characterization ◽  
Regulatory Elements ◽  
Plant Performance ◽  
Sugar Accumulation ◽  
Stress Regime ◽  
Diverse Range ◽  
Encoding Genes

Invertases are essential enzymes that irreversibly catalyze the cleavage of sucrose into glucose and fructose. Cell wall invertase (CWI) and vacuolar invertase (VI) are glycosylated proteins and exert fundamental roles in plant growth as well as in response to environmental cues. As yet, comprehensive insight into invertase encoding genes are lacking in Glycine max. In the present study, the systematic survey of gene structures, coding regions, regulatory elements, conserved motifs, and phylogenies resulted in the identification of thirty–two putative invertase genes in soybean genome. Concomitantly, impacts on gene expression, enzyme activities, proteins, and soluble sugar accumulation were explored in specific tissues upon stress perturbation. In combination with the observation of subcellular compartmentation of the fluorescent fusion protein that indeed exported to apoplast, heterologous expression, and purification in using Pichia pastoris system revealed that GmCWI4 was a typical extracellular invertase. We postulated that GmCWI4 may play regulatory roles and be involved in pathogenic fungi defense. The experimental evaluation of physiological significance via phenotypic analysis of mutants under stress exposure has been initiated. Moreover, our paper provides theoretical basis for elucidating molecular mechanisms of invertase in association with inhibitors underlying the stress regime, and will contribute to the improvement of plant performance to a diverse range of stressors.

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