scholarly journals Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548

2020 ◽  
Vol 8 (5) ◽  
pp. 711 ◽  
Author(s):  
Mekala Venkatachalam ◽  
Alain Shum-Chéong-Sing ◽  
Laurent Dufossé ◽  
Mireille Fouillaud
Keyword(s):  
Submerged Fermentation ◽  
Optimization Method ◽  
Agitation Speed ◽  
Interactive Effects ◽  
Potato Dextrose Broth ◽  
Fermentation Time ◽  
Maximum Production ◽  
Working Volume ◽  
Initial Ph ◽  
The Individual

Talaromyces albobiverticillius 30548 is a marine-derived pigment producing filamentous fungus, isolated from the La Réunion island, in the Indian Ocean. The objective of this study was to examine and optimize the submerged fermentation (SmF) process parameters such as initial pH (4–9), temperature (21–27 °C), agitation speed (100–200 rpm), and fermentation time (0–336 h), for maximum production of pigments (orange and red) and biomass, using the Box–Behnken Experimental Design and Response Surface Modeling (BBED and RSM). This methodology allowed consideration of multifactorial interactions between a set of parameters. Experiments were carried out based on the BBED using 250 mL shake flasks, with a 100 mL working volume of potato dextrose broth (PDB). From the experimental data, mathematical models were developed to predict the pigments and biomass yields. The individual and interactive effects of the process variables on the responses were also investigated (RSM). The optimal conditions for maximum production of pigments and biomass were derived by the numerical optimization method, as follows—initial pH of 6.4, temperature of 24 °C, agitation speed of 164 rpm, and fermentation time of 149 h, respectively.

scholarly journals Photocatalytic Treatment of An Actual Confectionery Wastewater Using Ag/TiO2/Fe2O3: Optimization of Photocatalytic Reactions Using Surface Response Methodology

Catalysts ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 409 ◽  
Author(s):  
Yi Lin ◽  
Mehrab Mehrvar
Keyword(s):  
Statistical Design ◽  
Optimization Method ◽  
Order Rate Constant ◽  
Light Wavelength ◽  
Statistical Design Of Experiments ◽  
Toc Removal ◽  
Initial Ph ◽  
Photocatalytic Reactions ◽  
The Individual ◽  
Independent Parameters

Titanium dioxide (TiO2) photocatalysis is one of the most commonly studied advanced oxidation processes (AOPs) for the mineralization of deleterious and recalcitrant compounds present in wastewater as it is stable, inexpensive, and effective. Out of all, doping with metal and non-metals, and the heterojunction with another semiconductor were proven to be efficient methods in enhancing the degradation of organic pollutants under ultraviolet (UV) and visible light. However, complex degradation processes in the treatment of an actual wastewater are difficult to model and optimize. In the present study, the application of a modified photocatalyst, Ag/TiO2/Fe2O3, for the degradation of an actual confectionery wastewater was investigated. Factorial studies and statistical design of experiments using the Box-Behnken method along with response surface methodology (RSM) were employed to identify the individual and cross-factor effects of independent parameters, including light wavelength (nm), photocatalyst concentration (g/L), initial pH, and initial total organic carbon (TOC) concentration (g/L). The maximum TOC removal at optimum conditions of light wavelength (254 nm), pH (4.68), photocatalyst dosage (480 mg/L), and initial TOC concentration (11,126.5 mg/L) was determined through the numerical optimization method (9.78%) and validated with experimental data (9.42%). Finally, the first-order rate constant with respect to TOC was found to be 0.0005 min−1 with a residual value of 0.998.

scholarly journals Photocatalytic Treatment of An Actual Confectionery Wastewater Using Ag/TiO2/Fe2O3: Optimization of Photocatalytic Reactions Using Surface Response Methodology.

2021 ◽  
Author(s):  
Yi-Ping Lin ◽  
Mehrab Mehrvar
Keyword(s):  
Statistical Design ◽  
Optimization Method ◽  
Order Rate Constant ◽  
Light Wavelength ◽  
Statistical Design Of Experiments ◽  
Toc Removal ◽  
Initial Ph ◽  
Photocatalytic Reactions ◽  
The Individual ◽  
Independent Parameters

Titanium dioxide (TiO2) photocatalysis is one of the most commonly studied advanced oxidation processes (AOPs) for the mineralization of deleterious and recalcitrant compounds present in wastewater as it is stable, inexpensive, and effective. Out of all, doping with metal and non-metals, and the heterojunction with another semiconductor were proven to be efficient methods in enhancing the degradation of organic pollutants under ultraviolet (UV) and visible light. However, complex degradation processes in the treatment of an actual wastewater are difficult to model and optimize. In the present study, the application of a modified photocatalyst, Ag/TiO2/Fe2O3, for the degradation of an actual confectionery wastewater was investigated. Factorial studies and statistical design of experiments using the Box-Behnken method along with response surface methodology (RSM) were employed to identify the individual and cross-factor effects of independent parameters, including light wavelength (nm), photocatalyst concentration (g/L), initial pH, and initial total organic carbon (TOC) concentration (g/L). The maximum TOC removal at optimum conditions of light wavelength (254 nm), pH (4.68), photocatalyst dosage (480 mg/L), and initial TOC concentration (11,126.5 mg/L) was determined through the numerical optimization method (9.78%) and validated with experimental data (9.42%). Finally, the first-order rate constant with respect to TOC was found to be 0.0005 min−1 with a residual value of 0.998.

scholarly journals Photocatalytic Treatment of An Actual Confectionery Wastewater Using Ag/TiO2/Fe2O3: Optimization of Photocatalytic Reactions Using Surface Response Methodology.

2021 ◽  
Author(s):  
Yi-Ping Lin ◽  
Mehrab Mehrvar
Keyword(s):  
Statistical Design ◽  
Optimization Method ◽  
Order Rate Constant ◽  
Light Wavelength ◽  
Statistical Design Of Experiments ◽  
Toc Removal ◽  
Initial Ph ◽  
Photocatalytic Reactions ◽  
The Individual ◽  
Independent Parameters

Titanium dioxide (TiO2) photocatalysis is one of the most commonly studied advanced oxidation processes (AOPs) for the mineralization of deleterious and recalcitrant compounds present in wastewater as it is stable, inexpensive, and effective. Out of all, doping with metal and non-metals, and the heterojunction with another semiconductor were proven to be efficient methods in enhancing the degradation of organic pollutants under ultraviolet (UV) and visible light. However, complex degradation processes in the treatment of an actual wastewater are difficult to model and optimize. In the present study, the application of a modified photocatalyst, Ag/TiO2/Fe2O3, for the degradation of an actual confectionery wastewater was investigated. Factorial studies and statistical design of experiments using the Box-Behnken method along with response surface methodology (RSM) were employed to identify the individual and cross-factor effects of independent parameters, including light wavelength (nm), photocatalyst concentration (g/L), initial pH, and initial total organic carbon (TOC) concentration (g/L). The maximum TOC removal at optimum conditions of light wavelength (254 nm), pH (4.68), photocatalyst dosage (480 mg/L), and initial TOC concentration (11,126.5 mg/L) was determined through the numerical optimization method (9.78%) and validated with experimental data (9.42%). Finally, the first-order rate constant with respect to TOC was found to be 0.0005 min−1 with a residual value of 0.998.

Pengaruh Penambahan Molases Pada Kulit Pisang Kapendis Untuk Media Produksi Xilanase B.Stearothermophillus DSM 22

2019 ◽  
Vol 15 (3) ◽  
Author(s):  
Trismillah
Keyword(s):  
Enzyme Activity ◽  
Ammonium Sulfate ◽  
Banana Peel ◽  
Partial Purification ◽  
Temperature Conditions ◽  
Cavendish Banana ◽  
Working Volume ◽  
Initial Ph ◽  
Xylanase Enzyme

Cavendish banana peel can be used as a substitute for the expensive xylan, while molasses than as a source of carbon as well as nitrogen, minerals and nutrients needed for the growth of microbes that can produce the enzyme. Xylanase produced from Bacillus stearothermopillus DSM 22, using media cavendish banana peels with the addition of molasses 1%, 2%, and 3%. Fermentation is done in a shaker incubator at 550C temperature conditions, initial pH 8, and 250 rpm agitation. The result showed the highest enzyme activity of 4,14 ± 0,16 U/mL min., on the addition 2% molasses after 24 hours. Further fermentation carried out in the fermenter working volume of 3.5 liters, with the condition of temperature 550C, pH 8, aeration 1 vvm, agitation 250 rpm, the highest spesific enzyme of activity of 51,62 ± 0,16 U/mg after 24 hours. Partial purification of xylanase enzyme fermentation is done with the results of microfiltration, ultrafiltration, ammonium sulfate (0-80%) and dialysis. There is an increase in the purity of the enzyme at each stage of purification, the highest purity on dialysis 3.23 times of crude enzymes.Kulit buah pisang kapendis dapat digunakan sebagai pengganti xilan yang harganya mahal, sementara molases selain sebagai sumber karbon serta nitrogen, mineral dan nutrisi dibutuhkan untuk pertumbuhan mikroba yang dapat menghasilkan enzim. Xilanase yang dihasilkan dari Bacillus stearothermopillus DSM 22, menggunakan media kulit pisang kapendis dengan penambahan molase 1%, 2%, dan 3%. Fermentasi dilakukan dalam shaker inkubator pada temperatur 550C, pH awal 8, dan agitasi 250 rpm. Hasilnya menunjukkan aktivitas enzim tertinggi 4,14 ± 0,16 U/mL min., pada penambahan 2% molases setelah 24 jam. Selanjutnya fermentasi dilakukan di dalam fermentor, volume kerja dari 3,5 liter, dengan kondisi temperatur 550C, pH 8, aeration 1 vvm, agitasi 250 rpm, aktivitas spesifik tertinggi 51,62 ± 0,16 U/mg setelah 24 jam. Pemurnian parsial fermentasi enzim xilanase dilakukan dengan hasil mikrofiltrasi, ultrafiltrasi, amonium sulfat (0-80%) dan dialisis. Ada peningkatan kemurnian enzim pada setiap tahap pemurnian, kemurnian tertinggi pada dialisis 3,23 kali dari enzim kasar.Keywords: Xylanase, B. stearothermophillus DSM 22, Cavendish banana peel, molasses, enzyme activity

scholarly journals Interactive effects between travel behaviour and COVID-19: a questionnaire study

2021 ◽  
Author(s):  
Jinbao Zhang ◽  
Jaeyoung Lee
Keyword(s):  
Early Stage ◽  
Travel Behaviour ◽  
Interactive Effects ◽  
Factors Affecting ◽  
Individual Level ◽  
Passenger Volume ◽  
Spatial Autoregressive ◽  
Railway Passenger ◽  
The Individual ◽  
And Control

Abstract This study has two main objectives: (i) to analyse the effect of travel characteristics on the spreading of disease, and (ii) to determine the effect of COVID-19 on travel behaviour at the individual level. First, the study analyses the effect of passenger volume and the proportions of different modes of travel on the spread of COVID-19 in the early stage. The developed spatial autoregressive model shows that total passenger volume and proportions of air and railway passenger volumes are positively associated with the cumulative confirmed cases. Second, a questionnaire is analysed to determine changes in travel behaviour after COVID-19. The results indicate that the number of total trips considerably decreased. Public transport usage decreased by 20.5%, while private car usage increased by 6.4%. Then the factors affecting the changes in travel behaviour are analysed by logit models. The findings reveal significant factors, including gender, occupation and travel restriction. It is expected that the findings from this study would be helpful for management and control of traffic during a pandemic.

scholarly journals Lovastatin Production byAspergillus terreusUsing Agro-Biomass as Substrate in Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Yin Wan Ho ◽  
Rosfarizan Mohamad ◽  
Yong Meng Goh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Initial Moisture Content ◽  
Fermentation Time ◽  
Maximum Production ◽  
Additional Nitrogen

Ability of two strains ofAspergillus terreus(ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained usingA. terreusATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM forA. terreusATCC 20542 andA. terreusATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P<0.01) and inoculums size and pH had no significant effect on lovastatin production (P>0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM forA. terreusATCC 20542 and ATCC 74135, respectively, using RS as substrate.

Optimization of the Fermentation Conditions for 1-Deoxynojirimycin Production by Streptomyces lawendulae Applying the Response Surface Methodology

2011 ◽  
Vol 7 (3) ◽  
Author(s):  
Zhao-Jun Wei ◽  
Le-Chun Zhou ◽  
Hua Chen ◽  
Gui-Hai Chen
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Starch Content ◽  
Ultra Violet ◽  
Soluble Starch ◽  
High Yield ◽  
Yield Strain ◽  
Fermentation Time ◽  
Ethyl Sulfate ◽  
Initial Ph

Moranoline (1-Deoxynojirimycin, DNJ) is a piperidine alkaloid, and shows high inhibit activities to glucoamylase and ?-glucosidase. One DNJ high-yield strain of Streptomyces lawendulae was obtained after isolated form soil and mutated with the ultra violet (UV) and ethyl sulfate (DES), which named as TB-412, and can produce DNJ with 35.925 mg/L. Response surface methodology (RSM) was applied to optimize the parameters of DNJ yield from S. lawendulae TB-412. The effects of independent variables of fermentation, including time, temperature, initial pH and the soluble starch content were investigated. The statistical analysis showed that the fermentation time, pH and the soluble starch content, and the quadratics of time, temperature, pH and the soluble starch content, as well as the interactions between fermentation time and pH, and time and the soluble starch content, showed significant effects on DNJ yield. The optimal process parameters for DNJ production within the experimental range of the variables researched was at 11d, 27 °C, pH 7.5, and 8% soluble starch content. At this condition, the DNJ yield was predicted to be 42.875 mg/L.

scholarly journals Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

REAKTOR ◽  
2017 ◽  
Vol 5 (2) ◽  
pp. 79
Author(s):  
Abdullah Abdullah ◽  
H. B. Mat
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Raw Material ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Pineapple Waste ◽  
Fermentation Liquid ◽  
Initial Ph

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

Heat Transfer in Reciprocating Planar Curved Tube With Piston Cooling Application

2005 ◽  
Vol 128 (1) ◽  
pp. 219-229 ◽  
Author(s):  
Shyy Woei Chang ◽  
Yao Zheng
Keyword(s):  
Heat Transfer ◽  
Local Heat Transfer ◽  
Local Heat ◽  
Interactive Effects ◽  
Experimental Conditions ◽  
Nusselt Numbers ◽  
Curved Tube ◽  
The Individual ◽  
Cooling Passage ◽  
Piston Cooling

This paper describes an experimental study of heat transfer in a reciprocating planar curved tube that simulates a cooling passage in piston. The coupled inertial, centrifugal, and reciprocating forces in the reciprocating curved tube interact with buoyancy to exhibit a synergistic effect on heat transfer. For the present experimental conditions, the local Nusselt numbers in the reciprocating curved tube are in the range of 0.6–1.15 times of static tube levels. Without buoyancy interaction, the coupled reciprocating and centrifugal force effect causes the heat transfer to be initially reduced from the static level but recovered when the reciprocating force is further increased. Heat transfer improvement and impediment could be superimposed by the location-dependent buoyancy effect. The empirical heat transfer correlation has been developed to permit the evaluation of the individual and interactive effects of inertial, centrifugal, and reciprocating forces with and without buoyancy interaction on local heat transfer in a reciprocating planar curved tube.

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