scholarly journals Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

2020 ◽  
Vol 8 (4) ◽  
pp. 601 ◽  
Author(s):  
Qian Song ◽  
Xun Deng ◽  
Rui-Qing Song
Keyword(s):  
Pichia Pastoris ◽  
Corn Stover ◽  
Pleurotus Ostreatus ◽  
Degradation Rate ◽  
Degradation Mechanism ◽  
White Rot Fungi ◽  
White Rot ◽  
Alkali Resistance ◽  
Good Thermal Stability ◽  
Recombinant Laccase

Pleurotus ostreatus is a species of white-rot fungi that effectively degrades lignin. In this study, we aimed to efficiently express the lac-2 gene of Pleurotus ostreatus in the Pichia pastoris X33 yeast strain. The enzymatic properties of recombinant yeast were determined, and its ability to degrade corn stover lignin was determined. The results showed the optimum pH values of recombinant laccase for 2,2’-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid, 2,6-dimethoxyphenol, and 2-methoxyphenol were 3.0, 3.0, and 3.5, respectively. The optimum reaction temperature was 50 °C, and it had good thermal stability and acid and alkali resistance. The degradation rate of lignin in corn stover by recombinant laccase was 18.36%, and the native Pleurotus ostreatus degradation rate was 14.05%, the difference between them is significant (p < 0.05). This experiment lays a foundation for the study of the degradation mechanism of lignin by laccase.

scholarly journals Enzyme degradation mechanism of white rot fungi and its research progress on Refractory Wastewater

2021 ◽  
Vol 237 ◽  
pp. 01002
Author(s):  
ShiYuan Huang ◽  
Sheng Li ◽  
ZhenYu Wang ◽  
SenHuan Lin ◽  
Jian Deng
Keyword(s):  
Phenolic Compounds ◽  
Enzymatic Degradation ◽  
Enzyme System ◽  
Degradation Mechanism ◽  
White Rot Fungi ◽  
White Rot ◽  
Research Progress ◽  
Degrading Enzyme ◽  
Research Status ◽  
Enzyme Degradation

The lignin-degrading enzyme system of white rot fungi is highly efficient and non-specific, and can degrade a variety of pollutants, including dyes, phenolic compounds and pesticides.The article presents an overview of the mechanism of enzymatic degradation of white rot fungi and its research status in several refractory wastewater were described.

Lignocellulolytic activity of Pleurotus ostreatus under solid state fermentation using silage, stover, and cobs of maize

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3797-3807
Author(s):  
Magdah Ganash ◽  
Tarek M. Abdel Ghany ◽  
Mohamed A. Al Abboud ◽  
Mohamed M. Alawlaqi ◽  
Husam Qanash ◽  
...  
Keyword(s):  
Solid State ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
Value Added ◽  
Extracellular Protein ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulosic Substrate

Lignocellulolytic white-rot fungi allow the bioconversion of agricultural wastes into value-added products that are used in a myriad of applications. The aim of this work was to use corn residues (Zea mays L.) to produce valuable products under solid-state fermentation (SSF) with Pleurotus ostreatus. White-rot fungus P. ostreatus was isolated from maize silage (MS) and thereafter it was inoculated on MS as substrate and compared with maize stover (MSt) and maize cobs (MC) to determine the best lignocellulosic substrate for the production of lignocellulolytic enzymes and extracellular protein. The MS gave the highest productivity of CMCase (368.2 U/mL), FPase (170.5 U/mL), laccase (11.4 U/mL), and MnPase (6.6 U/mL). This is compared to productivity on MSt of 222 U/mL, 50.2 U/mL, 4.55 U/mL, and 2.57 U/mL, respectively; and productivity on MC at the same incubation period as 150.5 U/mL, 48.2 U/mL, 3.58 U/mL, and 2.5 U/mL, respectively. The levels of enzyme production declined with increasing incubation period after 15 and 20 days using MS and MC, respectively, as substrates. Maximum liberated extracellular protein content (754 to 878 µg/mL) was recorded using MS, while a low amount (343 to 408 µg/mL) was liberated with using MSt and MC.

Evaluation of two wild types of Pleurotus ostreatus (MCC07 and MCC20) isolated from nature for their ability to decolorize Benazol Black ZN textile dye in comparison to some commercial types of white rot fungi: Pleurotus ostreatus, Pleurotus djamor, and Pleurotus citrinopileatus

2008 ◽  
Vol 54 (5) ◽  
pp. 366-370 ◽  
Author(s):  
Erbil Kalmış ◽  
Nuri Azbar ◽  
Fatih Kalyoncu
Keyword(s):  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
White Rot ◽  
Textile Dye ◽  
Wild Type ◽  
Pleurotus Djamor ◽  
Decolorization Efficiency ◽  
Pleurotus Citrinopileatus

Biological decolorization of Benazol Black ZN, a reactive azo-type textile dyestuff, was comparatively studied using 3 different commercial-type white rot fungi strains ( Pleurotus ostreatus , Pleurotus cornucopiae var. citrinopileatus, Pleurotus djamor , and 2 wild types of P. ostreatus (MCC07 and MCC20) isolated from the nature. The initial dye concentrations in the medium were 500 and 1000 mg·L–1. All the organisms studied decolorized Benazol Black ZN to varying degrees. At low dye concentration, both commercial and wild type of P. ostreatus resulted in the best decolorization, conversely, wild-type P. ostreatus (MCC07) was found to be much more robust against increasing dye concentration and provided the best decolorization efficiency at high dye concentration.

Production of cellulases and xylanases by white-rot fungi cultured in corn stover media for ruminant feed applications

2016 ◽  
Vol 221 ◽  
pp. 147-156 ◽  
Author(s):  
Deli Nazmín Tirado-González ◽  
Juan Jáuregui-Rincón ◽  
Gustavo G. Tirado-Estrada ◽  
Pedro Arturo Martínez-Hernández ◽  
Fidel Guevara-Lara ◽  
...  
Keyword(s):  
Corn Stover ◽  
White Rot Fungi ◽  
White Rot ◽  
Ruminant Feed

scholarly journals Biodegradation of nitroaromatic compounds in Red Water by white rot fungi Pleurotus ostreatus and floridae

2020 ◽  
Vol 15 (6) ◽  
pp. 1
Author(s):  
Cristiane Patrícia Kist ◽  
Claudio Eduardo Scherer ◽  
Marlene Soares ◽  
Marcio Barreto Rodrigues
Keyword(s):  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
Nitroaromatic Compounds ◽  
Maximum Activity ◽  
White Rot ◽  
Mn Peroxidase ◽  
Multivariate Study ◽  
Toxicity Factor ◽  
Peroxidase Enzymes

Pleurotus fungi are basidiomycetes that stand out in the degradation of recalcitrant organic compounds such as lignin derivatives and phenolic compounds. The aim of this study was to make a comparative evaluation of the capacity of the Pleurotus ostreatus POS 560 and Pleurotus floridae PSP1 fungi in the degradation of 2,4 and 2,6-dinitrotoluene (DNTs) in effluent from an explosive factory. The characterization of the effluent indicated 318 mg L-1 of DNTs, 246 mg L-1 of COD and toxicity factor for Daphnia magna corresponding to 8. The conduct of a multivariate study estimated the influence of the variables pH (5.0 and 6.0), co-substrate concentration (10 and 20 g L-1 of glucose) and species of the fungus Pleurotus (ostreatus and floridae) on the degradation of DNTs, indicating that the variables Fungus and [Glucose] were significant (p <0.05) presenting effects in the order of + 4.45 ± 0.26 and -1.14 ± 0.26, respectively. The reproduction of the best efficiency conditions (P. floridae; pH 6.0 and 10 g L-1 of glucose) in agitated flasks (100 rpm, 26oC) was able to carry out, within 14 days of treatment, the removal of organic matter and toxicity factor in levels on the order of 55 and 50%, respectively, in addition to the complete degradation of DNTs which occurred in the first 120 hours of treatment. In this period, the maximum activity of the peroxidase and Mn-peroxidase enzymes was also characterized, suggesting high potential of the bioprocess under study for remediation of effluents contaminated with nitroaromatic compounds.

scholarly journals Assessment of anammox, microalgae and white-rot fungi-based processes for the treatment of textile wastewater

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0247452
Author(s):  
Micol Bellucci ◽  
Francesca Marazzi ◽  
Alida Musatti ◽  
Riccardo Fornaroli ◽  
Andrea Turolla ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
Textile Wastewater ◽  
White Rot ◽  
Printing Industry ◽  
Batch Tests ◽  
Anammox Activity ◽  
Wastewater Samples ◽  
Textile Wastewaters

The treatability of seven wastewater samples generated by a textile digital printing industry was evaluated by employing 1) anammox-based processes for nitrogen removal 2) microalgae (Chlorella vulgaris) for nutrient uptake and biomass production 3) white-rot fungi (Pleurotus ostreatus and Phanerochaete chrysosporium) for decolorization and laccase activity. The biodegradative potential of each type of organism was determined in batch tests and correlated with the main characteristics of the textile wastewaters through statistical analyses. The maximum specific anammox activity ranged between 0.1 and 0.2 g N g VSS-1 d-1 depending on the sample of wastewater; the photosynthetic efficiency of the microalgae decreased up to 50% during the first 24 hours of contact with the textile wastewaters, but it improved from then on; Pleurotus ostreatus synthetized laccases and removed between 20–62% of the colour after 14 days, while the enzymatic activity of Phanerochaete chrysosporium was inhibited. Overall, the findings suggest that all microbes have great potential for the treatment and valorisation of textile wastewater after tailored adaptation phases. Yet, the depurative efficiency can be probably enhanced by combining the different processes in sequence.

scholarly journals PSXIV-14 Evaluation of 21 fungal strains as pretreatment of corn stover: chemical composition and in vitro digestibility

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 444-444
Author(s):  
Paulina Luna Moreno ◽  
Hermilo Leal-Lara ◽  
Águeda García-Pérez ◽  
Luis Corona ◽  
Atmir Romero-Pérez ◽  
...  
Keyword(s):  
Corn Stover ◽  
Lentinula Edodes ◽  
White Rot Fungi ◽  
Neutral Detergent Fiber ◽  
White Rot ◽  
In Vitro Digestibility ◽  
Edible Fungi ◽  
Fungal Strains ◽  
Dry Matter Digestibility

Abstract Agricultural by-products such as corn stover (CS) are abundantly available in many countries; however, its high content of neutral detergent fiber (NDF) and lignin (L) and low protein content, causes low digestibility and nutrient availability for ruminants. The production of edible fungi could improve the digestibility of the CS because fungi can break down the cross-links between cell wall components. The aim of the study was to evaluate the effect of the invaded CS-based substrate used in the production of 21 white rot fungi of the species: Auricularia sp (Auri), Ganoderma sp (Gano), Hericium sp (Heri), Lentinula edodes (LC, L5, L9, L15, L21), Pleurotus eryngii (Pe-PQ, Pe-MB), Pleurotus djamour (Pd-Pro, Pd-UTMR) and Pleurotus ostreatus (Po-IAP, Po-Psma, Po-P14, Po-POS, Po-IE202, Po-JP, Po-P35, Po-P38, Po-Sfco) on crude protein (CP), NDF and L content and in vitro dry matter digestibility (IVDMD). The variables were analyzed in a completely randomized design with the MIXED procedure of SAS and the comparison of the means was made with the Tukey analysis. For CP, a significant increase (P &lt; 0.05) was observed from 10% (Pe-MB) to 41% (LC) in comparison with the substrate without inoculation (C). There was a decrease (P &lt; 0.05) of NDF from 2% (LC) to 10% (Pd-PRO) against C. Fungal treated CS had no effect on L (P &lt; 0.05) as compared to C. Treatments increased (P &lt; 0.05) IVDMD from 3% (L15) up to 36% (Po-POS) for all fungal strains, except for Pe-PQ and Po-Sfco in comparison with C. There was a positive correlation (r2=0.21, P &lt; 0.05) between CP and IVDMD. An equation was obtained to predict IVDMD [IVDMD=0.40578 + 358.38(%ADF)–358.02(%NDF)+358.17(%HEM)–0.3211(%DM), R2=0.32 (P &lt; 0.05)]. In conclusion the mycelial invasion of white rot fungi on a CS-based substrate, increased CP content, decreased NDF and improved IVDMD, making it a viable option to increase the digestibility of CS.

scholarly journals PSXII-25 Characterization and evaluation of the in vitro dry matter digestibility of corn stover spent used to produce edible fungi

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 415-415
Author(s):  
Edgar García-Orozco ◽  
Gabriela Leyva-Olvera ◽  
Hermilo Leal-Lara ◽  
Atmir Romero-Pérez ◽  
Luis Corona ◽  
...  
Keyword(s):  
Corn Stover ◽  
Dry Matter ◽  
Lentinula Edodes ◽  
Animal Feed ◽  
White Rot Fungi ◽  
Neutral Detergent Fiber ◽  
White Rot ◽  
Edible Fungi ◽  
Dry Matter Digestibility

Abstract Corn stover (CS) is an agricultural byproduct available in many countries for animal feed. However, due to its high lignin (L) and neutral detergent fiber (NDF) content it has a low digestibility. The production of edible fungi, using CS-based substrate, is an option to improve CS digestibility, as fungi can break down the cross-links between cell wall components. The aim of the study was to evaluate the effect of fungal treated CS from 17 species of white rot fungi: Lentinula edodes (L5, L9, LC,L15), Hericium sp (Heri) and Pleurotus djamour (Pd-Pro, Pd-UTMR) Pleurotus eryngii (Pe-PQ, Pe-MB), Pleurotus ostreatus (Po-IE202, Po-IAP, Po-Psma, Po-P14, Po-POS, Po-JP, Po-P38, Po-Sfco) on crude protein (CP), L and NDF content and in vitro dry matter digestibility (IVDMD). The composition of the substrate (C) was CS (80.55%), ground sorghum (5.98%) wheat bran (3.89%), corn gluten (4.89%), CaCO3 (3.89%) and CaSO4 (0.89%). The variables were analyzed as a completely randomized design using the MIXED procedure of SAS. Means were compared with the Tukey procedure. A significant increase (P &lt; 0.05) of CP from 10% (Po-P38) to 69% (LC) was observed, except for Pd-UTMR, Po-IAP, Po-Psma, Po-P38 and Po-Sfco that showed similar content to the C. There was a decrease (P &lt; 0.05) in the NDF from 10% (Heri) to 34% (L15) in relation to the C. The L content did not decrease in comparison with the C (P &lt; 0.05). Furthermore, 13 treatments significantly increased (P &lt; 0.05) IVDMD from 24% (Po-P14) to 42% (Pd-UTMR), except for Po-P38, Po-JP, LC and Heri in comparison with the C. In conclusion, the cultivation of white rot fungi, such as Pd-UTMR, Po-IAP, Po-Psma, LC, L9 and L15 using CS as substrate, improved CP content and increased IVDMD of CS, suggesting that which can be a good alternative for feeding ruminants.

Sign in / Sign up

Export Citation Format

Share Document