scholarly journals Identification of Species and Subspecies of Lactic Acid Bacteria Present in Spanish Cheeses Type “Torta” by MALDI-TOF MS and pheS gene Analyses

2020 ◽  
Vol 8 (2) ◽  
pp. 301
Author(s):  
Fernando Sánchez-Juanes ◽  
Vanessa Teixeira-Martín ◽  
José Manuel González-Buitrago ◽  
Encarna Velázquez ◽  
José David Flores-Félix
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Taxonomic Status ◽  
Raw Milk ◽  
Animal Origin ◽  
Lactobacillus Curvatus ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Several artisanal cheeses are elaborated in European countries, being commonly curdled with rennets of animal origin. However, in some Spanish regions some cheeses of type “Torta” are elaborated using Cynara cardunculus L. rennets. Two of these cheeses, “Torta del Casar” and “Torta de Trujillo”, are elaborated in Cáceres province with ewe’s raw milk and matured over at least 60 days without starters. In this work, we identified the lactic acid bacteria present in these cheeses using MALDI-TOF MS and pheS gene analyses, which showed they belong to the species Lactobacillus curvatus, Lactobacillus diolivorans, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactococcus lactis and Leuconostoc mesenteroides. The pheS gene analysis also allowed the identification of the subspecies La. plantarum subsp. plantarum, La. paracasei subsp. paracasei and Le. mesenteroides subsp. jonggajibkimchii. Low similarity values were found in this gene for some currently accepted subspecies of Lc. lactis and for the two subspecies of La. plantarum, and values near to 100% for the subspecies of Le. mesenteroides and La. paracasei. These results, which were confirmed by the calculated ANIb and dDDH values of their whole genomes, showed the need to revise the taxonomic status of these species and their subspecies.

A brief identification of lactic acid bacteria isolated from Algerian goat’s Jben by MALDI-TOF MS

2018 ◽  
Vol 7 (4) ◽  
pp. 157-165
Author(s):  
Naïma L. Meghoufel ◽  
Abdelkader Homrani ◽  
Saïd Nemmiche ◽  
Nawel Benkrizi ◽  
Abdelkader E. Dahou ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Low Cost ◽  
Raw Milk ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Sub Saharan ◽  
Tof Ms

In this paper, we investigated the lactic acid bacteria’s community of the Algerian goat’s Jben in order to define and preserve it. This cheese variety is only handmade with raw milk, and a dried flower of Cynara cardunculus is used instead of the animal rennet, it is also restricted in sub-Saharan prov-inces in Algeria, and no previous studies on its lactic acid bacteria composi-tion have been performed before. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) procedure was used to identify 36 lactic acid bacteria isolated from artisanal Jben made from Arabia goat’s raw milk in Naâma (Algeria). The results showed the domination of the Enterococcus genus presents with four species: E. durans, E. faecalis, E. hirae and E. faecium. Lactococcus lactis and Leuconostoc mesenteroïdes were also detected. The species identified were favoured by the composition of goat’s raw milk microflora used and the artisanal manufacturing process of the cheese. The use of MALDI-TOF MS method provided us with a rapid diagnosis of the Jben lactic acid bacteria’s community. This method based on microbial abundant proteins fingerprint diagnosis was chosen for its fast, precise, low cost and less complicated analyse.

MALDI-TOF MS profiling of non-starter lactic acid bacteria from artisanal cheeses of the Greek island of Naxos

2020 ◽  
Vol 323 ◽  
pp. 108586 ◽  
Author(s):  
Charalampos Gantzias ◽  
Iliada K. Lappa ◽  
Maarten Aerts ◽  
Marina Georgalaki ◽  
Eugenia Manolopoulou ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Greek Island ◽  
Tof Ms

scholarly journals Maldi mass spectrometry for identification lactic acid bacilli isolated from lactic acid products produced in the Karagandy region

2021 ◽  
Vol 103 (3) ◽  
pp. 18-25
Author(s):  
Zhanar Amirkhanova ◽  
◽  
Rahat Bodeeva ◽  
Saule Akhmetova ◽  
Altynay Tuyakova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Mass Spectrometer ◽  
Cottage Cheese ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

At present the traditional methods of identifying microorganisms are replaced by express methods, the mass spectrometric method using MALDI-TOF MS allows to reliably identify a variety of microorganisms in a short time, which is an indisputable advantage in work and allows to quickly identify many microorganisms in quickly. Classical methods for the identification of lactic acid bacteria based on their cultivation require a long time for their implementation. The advent of matrix laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology made significant changes in the workflows for the study of lactic acid bacteria, unmatched in speed characteristics. This article presents the study of the morphological, cultural properties, acid-forming ability, antibiotic sensitivity lactic acid bacteria of isolated from products (ayran, koumiss, ashykan kozhe, kurt, suzbe, cottage cheese) produced in different districts of the Karaganda region. The species identification of the isolated strains of lactic acid bacteria was carried out using a MALDI-TOF mass spectrometer. The studies carried out on cultural and morphological characters indicate that they belong to the genus Lactobacillus, Lacticaseibacillus, Lactiplantibacillus, Limosilactobacillus. As a result of identification on the mass spectrometer of the presented samples L. acidophilus (2 strains), L. delbrueckii subsp. bulgaricum (2 strains), L. rhamnosus (7 strains), L. plantarum (2 strains), L. paracasei (11 strains), L. fermentum (2 strains) were revealed. According to the Score values, the results indicate the accuracy of the identification.

scholarly journals The Use of MALDI-TOF Mass Spectrometry, Ribotyping and Phenotypic Tests to Identify Lactic Acid Bacteria from Fermented Cereal Foods in Abidjan (Côte d’Ivoire)

2014 ◽  
Vol 8 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Amenan A Soro-Yao ◽  
Peter Schumann ◽  
Philippe Thonart ◽  
Koffi M Djè ◽  
Rüdiger Pukall
Keyword(s):  
Mass Spectrometry ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cote D'ivoire ◽  
Protein Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Automated Ribotyping ◽  
Phenotypic Tests ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) protein analysis, automated ribotyping, and phenotypic tests (e.g., cell morphology, gas production from glucose, growth and acid production on homofermemtative-heterofermentative differential (HHD) agar medium, sugar fermentation patterns) were used to identify 23 lactic acid bacteria (LAB) isolated from fermented cereal foods available in Abidjan, Côte d’Ivoire.Pediococcusacidilactici(56.5%),Lactobacillusfermentum(30.4%),L.salivarius(4.3%),P.pentosaceus(4.3%) andL. plantarumsubsp.plantarum(4.3%) were the species and subspecies identified. Protein based identification was confirmed by automated ribotyping for selected isolates and was similar to that provided by the phenotypic characterization. MALDI-TOF MS protein analysis provided a high level of discrimination among the isolates and could be used for the rapid screening of LAB starter cultures.

Melt Polycondensation ofd,l-Lactic Acid:  MALDI-TOF MS Investigation of the Ring-Chain Equilibrium

2001 ◽  
Vol 105 (14) ◽  
pp. 2833-2836 ◽  
Author(s):  
Sándor Kéki ◽  
Ildikó Bodnár ◽  
Jenő Borda ◽  
György Deák ◽  
Miklós Zsuga
Keyword(s):  
Lactic Acid ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Melt Polycondensation ◽  
Tof Ms

scholarly journals Identification of ovine-associated staphylococci by MALDI-TOF mass spectrometry

2021 ◽  
Author(s):  
A. Tonamo ◽  
I. Komlósi ◽  
L. Varga ◽  
M. Kačániová ◽  
F. Peles
Keyword(s):  
Mass Spectrometry ◽  
Raw Milk ◽  
Bacterial Isolates ◽  
Bulk Tank Milk ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bulk Tank ◽  
Raw Milk Cheese ◽  
Tof Ms

AbstractThe objective of this study was to use matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOF MS) for the identification of ovine-associated staphylococci. Presumptive Staphylococcus isolates were recovered from ovine udder surface (US), individual raw milk, bulk tank milk, and cheese samples and were characterised by conventional phenotypic methods. A total of 69 bacterial isolates were further confirmed by MALDI-TOF MS. Forty-two (60.9%) of 69 isolates were successfully identified on genus and species level. Two thirds (n = 28) of the 42 identified isolates were shown to be Staphylococcus spp. These 28 staphylococcal isolates formed two clusters, one consisting of 22 Staphylococcus aureus strains and the other composed of 6 non-aureus staphylococci, including S. simulans (n = 3), S. auricularis, S. equorum, and S. haemolyticus. MALDI-TOF MS has proven to be a reliable tool for the identification of staphylococci from raw sheep's milk, especially bulk tank milk; however, currently it appears to be less useful for the identification of bacterial isolates originating from ovine US samples. This is the first study to evaluate the applicability of MALDI-TOF MS for identification of Staphylococcus spp. in ovine raw milk, cheese, and US samples in Hungary.

Species-Level Discrimination of Psychrotrophic Pathogenic and Spoilage Gram-Negative Raw Milk Isolates Using a Combined MALDI-TOF MS Proteomics–Bioinformatics-based Approach

2017 ◽  
Vol 16 (6) ◽  
pp. 2188-2203 ◽  
Author(s):  
Nuwan R. Vithanage ◽  
Jeevana Bhongir ◽  
Snehal R. Jadhav ◽  
Chaminda S. Ranadheera ◽  
Enzo A. Palombo ◽  
...  
Keyword(s):  
Raw Milk ◽  
Species Level ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Acinetobacter variabilis sp. nov. (formerly DNA group 15 sensu Tjernberg & Ursing), isolated from humans and animals

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 857-863 ◽  
Author(s):  
Lenka Krizova ◽  
Jana McGinnis ◽  
Martina Maixnerova ◽  
Matej Nemec ◽  
Laurent Poirel ◽  
...  
Keyword(s):  
Carbon Sources ◽  
Taxonomic Status ◽  
Comparative Sequence Analysis ◽  
Whole Genome ◽  
Content Type ◽  
Maldi Tof Ms ◽  
Link Type ◽  
Maldi Tof ◽  
Group 15 ◽  
Tof Ms

We aimed to define the taxonomic status of 16 strains which were phenetically congruent with Acinetobacter DNA group 15 described by Tjernberg & Ursing in 1989. The strains were isolated from a variety of human and animal specimens in geographically distant places over the last three decades. Taxonomic analysis was based on an Acinetobacter -targeted, genus-wide approach that included the comparative sequence analysis of housekeeping, protein-coding genes, whole-cell profiling based on matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), an array of in-house physiological and metabolic tests, and whole-genome comparative analysis. Based on analyses of the rpoB and gyrB genes, the 16 strains formed respective, strongly supported clusters clearly separated from the other species of the genus Acinetobacter . The distinctness of the group at the species level was indicated by average nucleotide identity values of ≤82 % between the whole genome sequences of two of the 16 strains (NIPH 2171T and NIPH 899) and those of the known species. In addition, the coherence of the group was also supported by MALDI-TOF MS. All 16 strains were non-haemolytic and non-gelatinase-producing, grown at 41 °C and utilized a rather limited number of carbon sources. Virtually every strain displayed a unique combination of metabolic and physiological features. We conclude that the 16 strains represent a distinct species of the genus Acinetobacter , for which the name Acinetobacter variabilis sp. nov. is proposed to reflect its marked phenotypic heterogeneity. The type strain is NIPH 2171T ( = CIP 110486T = CCUG 26390T = CCM 8555T).

Sign in / Sign up

Export Citation Format

Share Document