scholarly journals Phenotypic and Genotypic Traits of Vancomycin-Resistant Enterococci from Healthy Food-Producing Animals

2020 ◽  
Vol 8 (2) ◽  
pp. 261 ◽  
Author(s):  
Valerie Wist ◽  
Marina Morach ◽  
Marianne Schneeberger ◽  
Nicole Cernela ◽  
Marc J.A. Stevens ◽  
...  
Keyword(s):  
Food Production ◽  
Healthy Food ◽  
Production Chain ◽  
Vana Gene ◽  
Vancomycin Resistant Enterococci ◽  
Healthy Cattle ◽  
Faecal Carriage ◽  
Different Types ◽  
Poultry Flock ◽  
Vancomycin Resistant

Food-producing animals may be a reservoir of vancomycin-resistant enterococci (VRE), potentially posing a threat to animal and public health. The aims of this study were to estimate the faecal carriage of VRE among healthy cattle (n = 362), pigs (n = 350), sheep (n = 218), and poultry (n = 102 flocks) in Switzerland, and to characterise phenotypic and genotypic traits of the isolates. VRE were isolated from caecum content of six bovine, and 12 porcine samples respectively, and from pooled faecal matter collected from 16 poultry flock samples. All isolates harboured vanA. Three different types of Tn1546-like elements carrying the vanA operon were identified. Conjugal transfer of vanA to human Enterococcus faecalis strain JH2-2 was observed for porcine isolates only. Resistance to tetracycline and erythromycin was frequent among the isolates. Our data show that VRE harbouring vanA are present in healthy food-producing animals. The vanA gene from porcine isolates was transferable to other enterococci and these isolates might play a role in the dissemination of VRE in the food production chain.

Vancomycin-resistant enterococci with vanA gene in treated municipal wastewater and their association with human hospital strains

2017 ◽  
Vol 609 ◽  
pp. 633-643 ◽  
Author(s):  
Veronika Oravcova ◽  
Matus Mihalcin ◽  
Jana Zakova ◽  
Lucie Pospisilova ◽  
Martina Masarikova ◽  
...  
Keyword(s):  
Municipal Wastewater ◽  
Vana Gene ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Treated Municipal Wastewater

scholarly journals Detection of vancomycin-resistant enterococci using chromogenic selective medium

2018 ◽  
Vol 20 (1) ◽  
pp. 55-61
Author(s):  
Anastasiya V. Fyodorova ◽  
Galina A. Klyasova
Keyword(s):  
Blood Culture ◽  
High Sensitivity ◽  
Selective Medium ◽  
Vana Gene ◽  
Microdilution Method ◽  
Vancomycin Resistant Enterococci ◽  
Broth Microdilution Method ◽  
Enterococcus Spp ◽  
Rectal Swabs ◽  
Vancomycin Resistant

Objective. To detect vancomycin-resistant enterococci (VRE) using chromogenic selective medium CHROMagar™VRE (CHROMagar, France). Materials and Methods. In the first part of the study, a total of 39 vancomycin-resistant and 20 vancomycinsusceptible Enterococcus spp. isolated from blood culture with known susceptibility profiles were incubated on the CHROMagar™VRE (CHROMagar, France) and examined after 24 h and 48 h of incubation. In the second part of the study, a total of 110 rectal swabs were taken from patients with hematological malignancies and incubated on the CHROMagar™VRE. The vancomycin susceptibility of isolates grown on the selective medium was further evaluated by the broth microdilution method (CLSI, 2017). Glycopeptide resistance genes were detected by PCR. Results. Using the CHROMagar™VRE, a total of 36 (92.3%) vancomycin-resistant isolates were detected after 24 h and additional two isolates – after 48 h of incubation. The sensitivity of the selective medium for detection of VRE obtained from blood culture was 92% and 97% after 24 h and 48 h of incubation, respectively. All 20 vancomycin-susceptible enterococci did not grow on the CHROMagar™VRE (specificity – 100%). Of 110 rectal swabs, 35 (31.8%) samples were positive for Enterococcus spp. on the CHROMagar™VRE (33 – E. faecium и 2 – E. faecalis). Resistance to vancomycin was detected in 32⁄33 (97%) E. faecium isolates, of them 28 and 4 strains were isolated after 24 h and 48 h of incubation; all VRE strains carried vanA gene. The proportion of false positive isolates was 3.4% after 24 h of incubation and 8.6% after 48 h of incubation on the CHROMagar™VRE medium for screening of VRE from rectal swabs. Conclusions. The chromogenic selective media CHROMagar™VRE has a high sensitivity and specificity for the detection of VRE and can be used for screening in laboratory practice.

scholarly journals Molecular Analysis of Tn1546-Like Elements in Vancomycin-Resistant Enterococci Isolated from Patients in Europe Shows Geographic Transposon Type Clustering

2001 ◽  
Vol 45 (3) ◽  
pp. 986-989 ◽  
Author(s):  
Marinus A. Schouten ◽  
Rob J. L. Willems ◽  
Wilma A. G. Kraak ◽  
Janetta Top ◽  
Jacomina A. A. Hoogkamp-Korstanje ◽  
...  
Keyword(s):  
United Kingdom ◽  
Molecular Analysis ◽  
Resistance Mechanism ◽  
Meat Consumption ◽  
The United Kingdom ◽  
Vancomycin Resistant Enterococci ◽  
Geographic Clustering ◽  
Different Types ◽  
Vancomycin Resistant ◽  
Evolutionary Lineages

ABSTRACT Resistance mechanism relatedness was studied in 18 clinical, European vanA vancomycin-resistant enterococci. Molecular analysis revealed 10 Tn1546-like elements, suggesting two evolutionary lineages. Lineage I dominated the European mainland, and lineage II dominated the United Kingdom and Israel. Geographic clustering reflected different types of meat consumption between countries, since each lineage is associated with colonization of different animals.

scholarly journals Mechanism of VanB Phenotype in Vancomycin-Resistant Enterococci carrying vanA gene

2006 ◽  
Vol 26 (6) ◽  
pp. 412-417 ◽  
Author(s):  
Il Joong Park ◽  
Wee Gyo Lee ◽  
Hyukmin Lee ◽  
Dongeun Yong ◽  
Kyungwon Lee ◽  
...  
Keyword(s):  
Vana Gene ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

scholarly journals Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil

2008 ◽  
Vol 50 (4) ◽  
pp. 195-198 ◽  
Author(s):  
Pedro Alves d'Azevedo ◽  
Guilherme H.C. Furtado ◽  
Eduardo A.S. Medeiros ◽  
Kelly Aline Santiago ◽  
Suzane Silbert ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Genetic Relatedness ◽  
Sao Paulo ◽  
São Paulo ◽  
Pfge Analysis ◽  
Predominant Species ◽  
Vana Gene ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.

scholarly journals Correction: Wist et al. Phenotypic and Genotypic Traits of Vancomycin-Resistant Enterococci from Healthy Food-Producing Animals. Microorganisms 2020, 8, 261

2021 ◽  
Vol 9 (4) ◽  
pp. 847
Author(s):  
Valerie Wist ◽  
Marina Morach ◽  
Marianne Schneeberger ◽  
Nicole Cernela ◽  
Marc J. A. Stevens ◽  
...  
Keyword(s):  
Healthy Food ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

The authors wish to make the following correction to this paper [...]

scholarly journals Identification of Vancomycin Resistance Determinants in Twin Cities of Rawalpindi/Islamabad

2021 ◽  
pp. 112-118
Author(s):  
Sidra Rehmat Ullah ◽  
Saadia Andleeb ◽  
Taskeen Raza ◽  
Khalid Mehmood
Keyword(s):  
Age Groups ◽  
Pcr Amplification ◽  
Vancomycin Resistance ◽  
Twin Cities ◽  
P Value ◽  
Blood Samples ◽  
Resistance Determinants ◽  
Vana Gene ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

Vancomycin resistant Enterococci have emerged rapidly in the recent years leading to treatment failure. The aim of this work was to identify vancomycin resistance determinants; vanA and vanB genes in Enterococci. The blood, urine and throat samples were collected from 150 patients from local hospitals of twin cities of Rawalpindi/Islamabad. Forty-nine phenotypically confirmed isolates were further confirmed by PCR amplification for vancomycin determinants (genes for vanA and vanB) for vancomycin resistant Enterococci. Dependence and frequency distribution of VRE and VSE bacteraemia with respect to age, gender and source was also studied. Phenotypically resistant strains were positive for vanA while negative for vanB. vancomycin susceptible enterococci (VSE) could be isolated more from urine samples as compared to blood whereas vancomycin resistant enterococci (VRE) was found more prevalent in the blood samples (p value= 0.000). VRE was more frequently isolated from patients aged 50 or above whereas VSE prevalence was same in both age groups (p value=0.002). Gender was not found to have any significant impact on VRE or VSE bacteraemia. This study reports vanA gene cluster responsible for resistance in Pakistani population and frequently isolation of VRE from blood samples. Keywords: Enterococcus, Vancomycin resistant Enterococci, Pakistan

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