scholarly journals The Root Nodule Microbiome of Cultivated and Wild Halophytic Legumes Showed Similar Diversity but Distinct Community Structure in Yellow River Delta Saline Soils

2020 ◽  
Vol 8 (2) ◽  
pp. 207 ◽  
Author(s):  
Yanfen Zheng ◽  
Jing Liang ◽  
Dong-Lin Zhao ◽  
Chen Meng ◽  
Zong-Chang Xu ◽  
...  

Symbiotic associations between leguminous plants and their nodule microbiome play a key role in sustainable agriculture by facilitating the fixation of atmospheric nitrogen and enhancing plant stress resistance. This study aimed to decipher the root nodule microbiome of two halophytic legumes, Sesbania cannabina and Glycine soja, which grow in saline soils of the Yellow River Delta, China, using PacBio’s circular consensus sequencing for full-length bacterial 16S rRNA gene to obtain finer taxonomic information. The cultivated legume Glycine max was used for comparison. We identified 18 bacterial genera and 55 species in nodule samples, which mainly classified to Proteobacteria, and rhizobial genus Ensifer was the predominant group. The three legumes showed similarity in operational taxonomic unit (OTU) diversity but distinction in OTU richness, indicating that they harbor similar bacterial species with different relative contents. The results of principal coordinates analysis and ANOSIM tests indicated that G. soja and G. max have similar nodule bacterial communities, and these communities differ from that of S. cannabina. Wild legumes S. cannabina and G. soja both harbored a higher number of rhizobia, while G. max possessed more non-rhizobial bacteria. These differences could be associated with their adaptability to saline–alkali stress and revealed clues on the nodule endophytes with relative importance of culturable rhizobial symbionts.

2020 ◽  
Vol 70 (12) ◽  
pp. 6284-6293 ◽  
Author(s):  
Shu-ge Wu ◽  
Jing-jing Wang ◽  
Jia-ning Wang ◽  
Qi Chen ◽  
Duo-hong Sheng ◽  
...  

A Gram-stain-negative, strictly aerobic, non-motile, orange-coloured bacterium, designated YR1-1T, was isolated from a soil sample collected from the Yellow River Delta wetlands (PR China). Growth was observed at a salinity of 1.0–15.0 % NaCl, 4–45 °C and pH 6.0–9.0. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that YR1-1T represented a member of the genus Psychroflexus , with the highest sequence similarity to Psychroflexus sediminis YIM-C238T (97.9 %), followed by Psychroflexus aestuariivivens (97.1 %) and Psychroflexus torquis (96.4 %). The average nucleotide identity and digital DNA–DNA hybridization values between YR1-1T and other closely related type strains of species of the genus Psychroflexus were 68.7–86.3% and 17.8–30.9 %. The genome of the strain was 2 899 374 bp in length with 39.8 % DNA G+C content. The predominant fatty acids (>10 %) were iso-C15 : 0 and anteiso-C15 : 0. The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, phospholipid, diphosphatidylglycerol, two unidentified aminolipids and four unidentified lipids. The combined genotypic and phenotypic data indicate that YR1-1T represents a novel species within the genus Psychroflexus , for which the name Psychroflexus aurantiacus sp. nov., is proposed. The type strain is YR1-1T (=KCTC 72794T=CGMCC 1.17458T).


2009 ◽  
Vol 17 (6) ◽  
pp. 1132-1136
Author(s):  
Qing-Mei LI ◽  
Long-Yu HOU ◽  
Yan LIU ◽  
Feng-Yun MA

2013 ◽  
Vol 37 (6) ◽  
pp. 503-516 ◽  
Author(s):  
Li-Qiong YANG ◽  
Guang-Xuan HAN ◽  
Jun-Bao YU ◽  
Li-Xin WU ◽  
Min ZHU ◽  
...  

2021 ◽  
Vol 9 (3) ◽  
pp. 270
Author(s):  
Meiyun Tang ◽  
Yonggang Jia ◽  
Shaotong Zhang ◽  
Chenxi Wang ◽  
Hanlu Liu

The silty seabed in the Yellow River Delta (YRD) is exposed to deposition, liquefaction, and reconsolidation repeatedly, during which seepage flows are crucial to the seabed strength. In extreme cases, seepage flows could cause seepage failure (SF) in the seabed, endangering the offshore structures. A critical condition exists for the occurrence of SF, i.e., the critical hydraulic gradient (icr). Compared with cohesionless sands, the icr of cohesive sediments is more complex, and no universal evaluation theory is available yet. The present work first improved a self-designed annular flume to avoid SF along the sidewall, then simulated the SF process of the seabed with different consolidation times in order to explore the icr of newly deposited silty seabed in the YRD. It is found that the theoretical formula for icr of cohesionless soil grossly underestimated the icr of cohesive soil. The icr range of silty seabed in the YRD was 8–16, which was significantly affected by the cohesion and was inversely proportional to the seabed fluidization degree. SF could “pump” the sediments vertically from the interior of the seabed with a contribution to sediment resuspension of up to 93.2–96.8%. The higher the consolidation degree, the smaller the contribution will be.


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