scholarly journals Exploiting the Advantages of Molecular Tools for the Monitoring of Fungal Indoor Air Contamination: First Detection of Exophiala jeanselmei in Indoor Air of Air-Conditioned Offices

2019 ◽  
Vol 7 (12) ◽  
pp. 674
Author(s):  
Xavier Libert ◽  
Camille Chasseur ◽  
Ann Packeu ◽  
Fabrice Bureau ◽  
Nancy H. Roosens ◽  
...  
Keyword(s):  
Indoor Air ◽  
Air Conditioning ◽  
Indoor Air Pollution ◽  
Airborne Fungi ◽  
Interesting Case ◽  
Public Health Issue ◽  
Molecular Tools ◽  
Exophiala Jeanselmei ◽  
Monitoring Protocols ◽  
Air Conditioning Systems

Today, indoor air pollution is considered a public health issue. Among the impacting pollutants, indoor airborne fungi are increasingly highlighted. Most of the monitoring protocols are culture-based, but these are unable to detect the uncultivable and/or dead fraction or species suppressed by fast-growing fungi, even though this fraction could impact health. Among the contaminants suspected to be part of this fraction, Exophiala jeanselmei is an interesting case study. Known to be pathogenic, this black yeast grows in humid environments such as air-conditioning systems, where it has been previously detected using classical culture-based methods. However, until now, this fungus was never detected in indoor air in contact with these air-conditioning systems. This study shows the first detection of E. jeanselmei in indoor air collected from offices in contact with contaminated air-conditioning reservoirs. While its presence in indoor air could not be demonstrated with culture-based methods, it was found by real-time PCR and massive parallel sequencing. The latter also allowed obtaining a broader view on the fungal diversity in the tested samples. Similar approaches were applied on water samples collected from the conditioning reservoirs to trace the source of contamination. The comparison of results obtained with both methods confirmed that the molecular tools could improve indoor air monitoring, especially of dead and/or uncultivable contaminants or when competition between species could occur.

Bioactive Nano-Filters to Control Legionella on Indoor Air

2012 ◽  
Vol 506 ◽  
pp. 23-26
Author(s):  
P.A.F. Rodrigues ◽  
S.I.V. Sousa ◽  
Maria José Geraldes ◽  
M.C.M. Alvim-Ferraz ◽  
F.G. Martins
Keyword(s):  
Air Pollution ◽  
Air Quality ◽  
Indoor Air Quality ◽  
Indoor Air ◽  
Legionella Pneumophila ◽  
Air Conditioning ◽  
Indoor Air Pollution ◽  
High Efficiency ◽  
Respiratory Infections ◽  
Public Health Issue

Several factors affect the indoor air quality, among which ventilation, human occupancy, cleaning products, equipment and material; they might induce the presence of aerosols (or bioaerosols in the presence of biological components) nitrogen oxides, ozone, carbon monoxide and dioxide, volatile organic compounds, radon and microorganisms. Microbiological pollution involves hundreds of bacteria and fungi species that grow indoors under specific conditions of temperature and humidity. Exposure to microbial contaminants is clinically associated with allergies, asthma, immune responses and respiratory infections, such as Legionnaires Disease and Pontiac Feaver, which are due to contamination byLegionella pneumophila. Legionnaire's Disease has increased over the past decade, because of the use of central air conditioning. In places such as homes, kindergartens, nursing homes and hospitals, indoor air pollution affects population groups that are particularly vulnerable because of their health status or age, making indoor air pollution a public health issue of high importance. Therefore, the implementation of preventive measures, as the application of air filters, is fundamental. Currently, High Efficiency Particulate Air (HEPA) filters are the most used to capture microorganisms in ventilation, filtration and air conditioning systems; nevertheless, as they are not completely secure, new filters should be developed. This work aims to present how the efficiency of a textile nanostructure in a non-woven material based on synthetic textiles (high hydrophobic fibers) incorporating appropriate biocides to controlLegionella pneumophila, is going to be measured. These bioactive structures, to be used in ventilation systems, as well as in respiratory protective equipment, will reduce the growth of microorganisms in the air through bactericidal or bacteriostatic action. The filter nanostructure should have good air permeability, since it has to guarantee minimum flows of fresh air for air exchange as well as acceptable indoor air quality.

scholarly journals Siekgebou-indroom - 'n krietiese oorsig

1991 ◽  
Vol 8 (3) ◽  
Author(s):  
C Leijeaar
Keyword(s):  
Air Pollution ◽  
Indoor Air ◽  
Tobacco Smoke ◽  
Air Conditioning ◽  
Indoor Air Pollution ◽  
Air Pollutant ◽  
Air Conditioning Systems

Worldwide, indoor air pollution is causing great concern. Because is often the only visible air pollutant, tobacco smoke is invariably singled out as the culprit. However, detailed research has found that it plays an insignificant role and that the blame should rather be placed on poorly designed and negligently maintained heating, ventilation and air conditioning systems. 

scholarly journals Indoor Air Quality Enhancement Performance of Liquid Desiccant and Evaporative Cooling-Assisted Air Conditioning Systems

2019 ◽  
Vol 11 (4) ◽  
pp. 1036 ◽  
Author(s):  
Beom-Jun Kim ◽  
Junseok Park ◽  
Jae-Weon Jeong
Keyword(s):  
Air Quality ◽  
Indoor Air Quality ◽  
Indoor Air ◽  
Reference System ◽  
Air Conditioning ◽  
Evaporative Cooling ◽  
Quality Enhancement ◽  
Liquid Desiccant ◽  
Air Conditioning Systems ◽  
Indoor Pm2.5

The main objective of this study is to investigate the indoor air quality enhancement performance of two different liquid desiccant and evaporative cooling-assisted air conditioning systems, such as the variable air volume (VAV) system with the desiccant-enhanced evaporative (DEVap) cooler, and the liquid desiccant system with an indirect and direct evaporative cooling-assisted 100% outdoor air system (LD-IDECOAS), compared with the conventional VAV system. The transient simulations of concentration variations of carbon dioxide (CO2), coarse particles, and fine particles (PM10 and PM2.5) in a model office space served by each system were performed using validated system models that were found in the literature. Based on the hourly thermal loads of the model space predicted by the TRNSYS 18 program, each air conditioning system was operated virtually using a commercial equation solver program (EES). The results indicated that the LD-IDECOAS provided the lowest annual indoor CO2 concentration among all the systems considered in this research, while the VAV system with DEVap cooler exceeded the threshold concentration (i.e., 1000 ppm) during the cooling season (i.e., July, August, and September). For the indoor particulate contaminant concentrations, both liquid desiccant and evaporative cooling-assisted air conditioning systems indicated lower indoor PM2.5 and PM10 concentrations compared with the reference system. The LD-IDECOAS and the VAV with a DEVap cooler demonstrated 33.3% and 23.5% lower annual accumulated indoor PM10 concentrations than the reference system, respectively. Similarly, the annual accumulated indoor PM2.5 concentration was reduced by 16% using the LD-IDECOAS and 17.1% using the VAV with DEVap cooler.

Fungal Contamination in Hospital Environments

2006 ◽  
Vol 27 (1) ◽  
pp. 44-47 ◽  
Author(s):  
F. Perdelli ◽  
M. L. Cristina ◽  
M. Sartini ◽  
A. M. Spagnolo ◽  
M. Dallera ◽  
...  
Keyword(s):  
Air Conditioning ◽  
Airborne Fungi ◽  
Selective Medium ◽  
Indoor Environments ◽  
Fungal Contamination ◽  
Mean Values ◽  
Hospital Environments ◽  
Airborne Concentration ◽  
Detection And Counting ◽  
Air Conditioning Systems

Objectives.To assess the degree of fungal contamination in hospital environments and to evaluate the ability of air conditioning systems to reduce such contamination.Methods.We monitored airborne microbial concentrations in various environments in 10 hospitals equipped with air conditioning. Sampling was performed with a portable Surface Air System impactor with replicate organism detection and counting plates containing a fungus-selective medium. The total fungal concentration was determined 72-120 hours after sampling. The genera most involved in infection were identified by macroscopic and microscopic observation.Results.The mean concentration of airborne fungi in the set of environments examined was 19 ± 19 colony-forming units (cfu) per cubic meter. Analysis of the fungal concentration in the different types of environments revealed different levels of contamination: the lowest mean values (12 ± 14 cfu/m3) were recorded in operating theaters, and the highest (45 ± 37 cfu/m3) were recorded in kitchens. Analyses revealed statistically significant differences between median values for the various environments. The fungal genus most commonly encountered was Penicillium, which, in kitchens, displayed the highest mean airborne concentration (8 ± 2.4 cfu/m3). The percentage (35%) of Aspergillus documented in the wards was higher than that in any of the other environments monitored.Conclusions.The fungal concentrations recorded in the present study are comparable to those recorded in other studies conducted in hospital environments and are considerably lower than those seen in other indoor environments that are not air conditioned. These findings demonstrate the effectiveness of air-handling systems in reducing fungal contamination.

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