scholarly journals Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

2019 ◽  
Vol 7 (9) ◽  
pp. 291 ◽  
Author(s):  
Rodrigo Rodriguez ◽  
Carla Santos ◽  
Marta F. Simões ◽  
Célia Soares ◽  
Cledir Santos ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Morphological Characteristics ◽  
Biotechnological Potential ◽  
Maldi Tof Ms ◽  
Aspergillus Section Nigri ◽  
Maldi Tof ◽  
Freeze Dried ◽  
Aspergillus Section ◽  
Tof Ms

This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent.

scholarly journals Novel attempt at discrimination of a bullet-shaped siphonophore (Family Diphyidae) using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-ToF MS)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nayeon Park ◽  
Jisu Yeom ◽  
Raehyuk Jeong ◽  
Wonchoel Lee
Keyword(s):  
Species Identification ◽  
Morphological Characteristics ◽  
Kuroshio Current ◽  
Northwest Pacific ◽  
Maldi Tof Ms ◽  
Protein Mass ◽  
Maldi Tof ◽  
Morphological Findings ◽  
Flight Mass Spectrometry ◽  
Tof Ms

AbstractOne major difficulty in identifying the gelatinous bodied bullet-shaped Siphonophore, Diphyids, is that their shape is deformed following ethanol fixation. Ethanol often is preferred over other fixatives, since samples fixed in ethanol can be used for molecular studies that can supplement morphological findings. To overcome this problem, we obtained protein mass spectra of ten species of Diphyidae found in the waters of the Kuroshio Current (Northwest Pacific and South Coast of South Korea) to test whether MALDI-ToF MS could be used as a methodology for species identification. In addition, a number of morphological characteristics that can be used with ethanol-treated samples was summarized. Concatenated phylogenetic analysis was also performed to determine the phylogenetic relationship by obtaining partial sequences of four genes (mtCOI, 16S rRNA, 18S rRNA, and ITS regions). Based on our integrative analysis, MALDI-ToF MS was evaluated as a potentially fast, inexpensive, and accurate tool for species identification along with conventional morphological and DNA barcoding for Diphyidae.

Cluster-based comparison of the peptide mass fingerprint obtained by MALDI-TOF mass spectrometry. A case study: long-term stability of rituximab

The Analyst ◽  
2015 ◽  
Vol 140 (5) ◽  
pp. 1717-1730 ◽  
Author(s):  
Pablo J. Villacorta ◽  
Antonio Salmerón-García ◽  
David A. Pelta ◽  
José Cabeza ◽  
Antonio Lario ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibodies ◽  
Peptide Mass Fingerprint ◽  
Maldi Tof Ms ◽  
Long Term Stability ◽  
Maldi Tof ◽  
Peptide Mass ◽  
Tof Ms

A cluster-based comparison algorithm applied to the MALDI-TOF-MS peptide mass fingerprint allows for tracking major changes in protein such as monoclonal antibodies.

scholarly journals Kerstersia gyiorumIsolated from a Bronchoalveolar Lavage in a Patient with a Chronic Tracheostomy

2014 ◽  
Vol 2014 ◽  
pp. 1-3 ◽  
Author(s):  
Meredith Deutscher ◽  
Jennifer Severing ◽  
Joan-Miquel Balada-Llasat
Keyword(s):  
Bronchoalveolar Lavage ◽  
Upper Respiratory Tract ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
The Matrix ◽  
History Of ◽  
Kerstersia Gyiorum ◽  
Tof Ms

The use of the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) generates rapid microbial identification. We are presenting a case of a 63-year-old woman with a medical history of chronic tracheostomy admitted for hypotension and fevers to illustrate the clinical implication of MALDI-TOF MS on bacterial identification.Kerstersia gyiorumwas identified from the bronchoalveolar lavage isolate.Kerstersia gyiorumhas been isolated from human sputum samples, and may be a previously unrecognized colonizer of the upper respiratory tract. Thus, patients with long-term tracheotomies or who are chronically aspirating may be at risk of lower respiratory infection with this organism. Increased use of MALDI-TOF MS in the clinical setting may increase reporting of this atypical isolate.

scholarly journals Use of a polyphasic approach including MALDI-TOF MS for identification of Aspergillus section Flavi strains isolated from food commodities in Brazil

2015 ◽  
Vol 65 (4) ◽  
pp. 2119-2129 ◽  
Author(s):  
Fernanda Chaves da Silva ◽  
Sara Maria Chalfoun ◽  
Luis Roberto Batista ◽  
Cledir Santos ◽  
Nelson Lima
Keyword(s):  
Polyphasic Approach ◽  
Maldi Tof Ms ◽  
Aspergillus Section Flavi ◽  
Maldi Tof ◽  
Food Commodities ◽  
Aspergillus Section ◽  
Tof Ms

scholarly journals Rapid MALDI-TOF MS identification of commercial truffles

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Khalid El Karkouri ◽  
Carine Couderc ◽  
Philippe Decloquement ◽  
Annick Abeille ◽  
Didier Raoult
Keyword(s):  
Forest Ecosystems ◽  
Morphological Characteristics ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser

AbstractTruffles are edible mushrooms with similar morphological characteristics, that make it difficult to distinguish between highly prized truffles (such as the Périgord black T. melanosporum) and inexpensive truffles (such as the Asian Black T. indicum). These biological and economic features have led to several misidentifications and/or fraudulent profit in the truffle markets. In this paper, we investigate Matrix-assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) biotyping to identify 34 commercial fresh truffles from Europe and Asia. The MALDI-TOF MS clustering rapidly distinguished seven Tuber species identified by ITS phylogenetic analysis. The tasty T. melanosporum was clearly differentiated from the Chinese and less expensive truffles. These cheaper mushrooms were marketed as T. indicum but corresponded to a mix of three species. In total, the method confirmed misidentifications in 26% of commercial specimens. Several unknown blind-coded truffles were rapidly identified, with scores >= 2, using the Bruker Biotyper algorithm against MS databases. This study demonstrates that MALDI-TOF MS is a reliable, rapid and cheaper new tool compared with molecular methods for the identification of truffle species and could be used to control frauds in the truffle markets. It could also be useful for the certification of truffle-inoculated seedlings and/or diversity in forest ecosystems.

scholarly journals Epidemiology and species distribution of anaerobic Gram-negative cocci: a 10-year retrospective survey (2008-2017)

2019 ◽  
Vol 89 (2) ◽  
pp. 84-87 ◽  
Author(s):  
Márió Gajdács ◽  
Edit Urbán
Keyword(s):  
Male Dominance ◽  
Clinical Samples ◽  
Diagnostic Efficacy ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Clinical Center ◽  
Causative Agents ◽  
Tof Ms

Introduction: The group of anaerobic Gram-negative cocci (AGNC) includes the genera Veillonella, Megasphaera, Anaeroglobus, Negativicoccus and Acidaminococcus. These bacteria are an integral part of the microbiome of humans but may be causative agents in various infectious processes. The available data on the epidemiology and significance of AGNCs is scarce. Aims: To assess and compare the prevalence of different species of AGNCs among inpatients and outpatients at the Albert Szent-Györgyi Clinical Center retrospectively, during a 10-year study period. Methods: Isolates containing AGNC were identified retrospectively by reviewing the online microbiology records of the Institute of Clinical Microbiology. Results: The median age of affected patients overall was 52 years (range: 1–90 years), with a male dominance. 59.79% of samples originated from inpatients. 572 individual AGNCs isolates were recovered from clinical samples, most of the isolated GNACs were Veillonella spp. (95.28%), Megasphaera and Acidaminococcus species accounted for a minority of isolates (2.79% and 1.93%, respectively), while Anaeroglobus and Negativicoccus species were not isolated. In the second half of the study period (2013-2017), 91.31% of isolates were identified on the species level (p<0.001) using MALDI-TOF MS. Conclusion: The current study represents a long-term surveillance study on the isolation frequency and trends among anaerobic Gram-negative cocci (AGNCs), isolated in the Southern Great Plain of Hungary, highlighting the beneficial effect of MALDI-TOF MS on the diagnostic efficacy of the laboratories.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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