scholarly journals The Anti-Rotaviral Activity of Low Molecular Weight and Non-Proteinaceous Substance from Bifidobacterium longum BORI Cell Extract

2019 ◽  
Vol 7 (4) ◽  
pp. 108
Author(s):  
Yeo Ok Han ◽  
Yunju Jeong ◽  
Hyun Ju You ◽  
Seockmo Ku ◽  
Geun Eog Ji ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Molecular Weight ◽  
Cytopathic Effect ◽  
Diarrheal Disease ◽  
Bifidobacterium Longum ◽  
Cell Extract ◽  
Host Cells ◽  
Low Molecular Weight ◽  
Protein Components ◽  
The Relationship

Rotavirus infection is the most common diarrheal disease worldwide in children under five years of age, and it often results in death. In recent years, research on the relationship between rotavirus and probiotics has shown that probiotics are effective against diarrhea. A clinical trial has reported that Bifidobacterium longum BORI reduced diarrhea induced by rotavirus. The present work investigated the anti-rotaviral effect of B. longum BORI by cytopathic effect observation and real time cell analyses. Our study found that B. longum BORI showed strong anti-rotaviral effect when incubated with MA104 cells prior to viral infection, suggesting that the probiotic does in fact interfere with the interaction of viruses and host cells. It is believed that the efficacy is due to low-molecular weight and non-protein components derived from B. longum BORI. This discovery can help broaden the industrial application of B. longum BORI, which has been proven to be a safe and effective probiotic.

scholarly journals A randomized clinical trial on the antitumoral effects of low molecular weight heparin in the treatment of esophageal cancer

2018 ◽  
Vol 234 (4) ◽  
pp. 4191-4199
Author(s):  
Ali Taghizadeh Kermani ◽  
Sare Hosseini ◽  
Azar Fanipakdel ◽  
Mona Joudi Mashhad ◽  
Kambiz Akhavan Rezayat ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Molecular Weight ◽  
Esophageal Cancer ◽  
Randomized Clinical Trial ◽  
Low Molecular Weight Heparin ◽  
Low Molecular Weight

Stress protein content of mature Brassica seeds and their germination performance

1998 ◽  
Vol 8 (3) ◽  
pp. 347-355 ◽  
Author(s):  
Mary Bettey ◽  
W. E. Finch-Savage
Keyword(s):  
Molecular Weight ◽  
Water Stress ◽  
Seed Development ◽  
Positive Relationship ◽  
Stress Proteins ◽  
Stress Protein ◽  
Low Molecular Weight ◽  
Optimal Conditions ◽  
Seed Vigour ◽  
The Relationship

AbstractPlants respond to sub-optimal conditions by the synthesis of specific ‘stress’ proteins, and these are thought to play a role in stress tolerance. Some of these proteins accumulate during late seed development, arguably to protect against damage during post-maturation drying and subsequent imbibition, prior to germination. Seed vigour is also determined during this late stage of seed development. High vigour seeds are those that can withstand the desiccation required for storage and successfully germinate under sub-optimal conditions to establish healthy seedlings. If stress proteins are involved in tolerating stress conditions, then they are likely to be important determinants of seed vigour. In this work the relationship between seed vigour (measured by seed germination performance following rapid aging, or under water stress) in Brassica oleracea var. capitata and the content of two classes of stress protein (dehydrins and a low molecular weight heat shock protein HSP17.6) at maturity was examined. Dehydrins did not show a positive relationship with seed performance. However, the protein HSP17.6 showed a positive correlation with seed performance, and a treatment that reduced the amount of this protein in the seed also caused a reduction in subsequent seed performance.

No effect of enoxaparin on outcome of aneurysmal subarachnoid hemorrhage: a randomized, double-blind, placebo-controlled clinical trial

2003 ◽  
Vol 99 (6) ◽  
pp. 953-959 ◽  
Author(s):  
Jari Siironen ◽  
Seppo Juvela ◽  
Joona Varis ◽  
Matti Porras ◽  
Kristiina Poussa ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Molecular Weight ◽  
Subarachnoid Hemorrhage ◽  
Low Molecular Weight Heparin ◽  
Aneurysmal Subarachnoid Hemorrhage ◽  
Intracranial Bleeding ◽  
Low Molecular Weight ◽  
Double Blind ◽  
Content Type ◽  
Fine Print

Object. From the moment an intracranial aneurysm ruptures, cerebral blood flow is impaired, and this impairment mainly determines the outcome in patients who survive after the initial bleeding. The exact mechanism of impairment is unknown, but activation of coagulation and fibrinolysis correlate with clinical condition and outcome after aneurysmal subarachnoid hemorrhage (SAH). The purpose of this study was to determine whether enoxaparin, a low-molecular-weight heparin, which is a well-known anticoagulating agent, has any effect on the outcome of aneurysmal SAH postoperatively. Methods. In this randomized, double-blind, single-center clinical trial, 170 patients (85 per group) with aneurysmal SAH were randomly assigned to receive either enoxaparin (40 mg subcutaneously once daily) or a placebo, starting within 24 hours after occlusion of the aneurysm and continuing for 10 days. Analysis was done on an intention-to-treat basis. Outcome was assessed at 3 months on both the Glasgow Outcome and modified Rankin Scales. Patients were eligible for the study if surgery was performed within 48 hours post-SAH, and no intracerebral hemorrhage was larger than 20 mm in diameter on the first postoperative computerized tomography scan. At 3 months, there were no significant differences in outcome by treatment group. Of the 170 patients, 11 (6%) died, and only 95 (56%) had a good outcome. Principal causes of unfavorable outcome were poor initial condition, delayed cerebral ischemia, and surgical complications. There were four patients with additional intracranial bleeding in the group receiving enoxaparin. The bleeding was not necessarily associated with the treatment itself, nor did it require treatment, and there were no such patients in the placebo group. Conclusions. Enoxaparin seemed to have no effect on the outcome of aneurysmal SAH in patients who had already received routine nimodipine and who had received triple-H therapy when needed. Routine use of low-molecular-weight heparin should be avoided during the early postoperative period in patients with SAH, because this agent seems to increase intracranial bleeding complications slightly, with no beneficial effect on neurological outcome.

FRACTIONATION OF LIVER SOMATOMEDIN ACTIVITY BY ULTRAFILTRATION

1974 ◽  
Vol 62 (2) ◽  
pp. 355-361 ◽  
Author(s):  
JENNIFER M. DEHNEL ◽  
P. D. McCONAGHEY ◽  
M. J. O. FRANCIS
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Weight Fraction ◽  
Low Molecular Weight ◽  
High Molecular Weight Fraction ◽  
Cartilage Growth ◽  
The Relationship ◽  
Somatomedin Inhibitors

SUMMARY Plasma somatomedin is the intermediary through which growth hormone (GH) exerts its effects on the growing skeleton. Somatomedin activity may be produced in vitro by perfusion of the liver and kidneys of rats with Waymouth's medium containing GH. The relationship between the activity of plasma somatomedin and somatomedin of hepatic and renal origin has yet to be clarified. Somatomedin from plasma can be separated into active fractions of both high and low molecular weight. Similarly, ultrafiltration of medium containing somatomedin of hepatic origin indicates the existence of two active fractions, one of high molecular weight (greater than 50000) and one of low molecular weight (less than 1000). The latter can be attributed to the release of amino acids, such as serine and glutamine, by the perfused tissue. The high molecular weight fraction is believed to represent GH-dependent somatomedin. Fractions that inhibit production of cartilage matrix are present in liver perfusates as well as in plasma. These results provide further evidence that the liver is a source of GH-dependent somatomedin in vivo. Furthermore, cartilage growth may be controlled not only by the GH-stimulated release of somatomedin by the liver, but also by its release of acid-labile somatomedin inhibitors.

Biological activity of 2,4-D esters on rubber vine (Cryptostegia grandiflora): dependence on vapour pressure and molecular weight and isomerism of the alcohol substituent

1989 ◽  
Vol 40 (3) ◽  
pp. 685
Author(s):  
GJ Harvey
Keyword(s):  
Molecular Weight ◽  
Vapour Pressure ◽  
Low Molecular Weight ◽  
Straight Chain ◽  
Biological Efficacy ◽  
Cryptostegia Grandiflora ◽  
Normal Series ◽  
Branched Chain ◽  
The Relationship ◽  
Volatile Esters

The relationship between molecular structure and biological efficacy was investigated for 16 esters of 2,4-D [(2,4-dich1orophenoxy)acetic acid] on rubber vine (Cryptostegia grandiflora). These included the normal (n) or straight-chain esters from C-1 (methyl) to C-8 (octyl), the n-decyl, n-dodecyl, isobutyl, amyl (iso-pentyl), 2-ethylhexyl (iso-octyl), and the methoxy-, ethoxy-, and butoxyethyl esters. For the normal series esters, biological efficacy was found to be a function of both the molecular weight and the vapour pressure of the esters. This relationship was linear for the higher molecular weight, low volatile esters, biological efficacy decreasing with increasing molecular weight and the accompanying decrease in vapour pressure of the esters. The low molecular weight, volatile esters were more active than the higher molecular weight, low volatile esters, and increases in the vapour pressure of these low molecular weight, volatile esters were sufficient to account for the deviation from linearity of those esters. When all esters are considered, the same relationships hold but the branched-chain (iso) and chemically substituted (alkoxy alcohol) esters are less effective than the corresponding normal esters. Possible reasons for these results are discussed.

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