scholarly journals Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM)

Micromachines ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 460 ◽  
Author(s):  
Nhu Tran ◽  
Kieu Trinh ◽  
Jun-Ho Lee ◽  
Won Yoon ◽  
Heongkyu Ju
Keyword(s):  
Surface Plasmons ◽  
Surface Plasmon ◽  
Coefficient Of Variation ◽  
Fluorescence Enhancement ◽  
Fluorescence Emission ◽  
High Reproducibility ◽  
Practical Applications ◽  
Biochemical Assays ◽  
Gold Silver ◽  
Spectral Channels

We demonstrate the enhancement of fluorescence emission from a dye, 5-carboxyfluorescein (FAM), which couples with surface plasmons at the spectral channels of excitation and emission. Experiments and calculations revealed that bimetallic (gold-silver) plasmon, as compared to the monometallic ones, allowed such coupling to be enhanced, at both the spectral channels. We achieved the maximum fluorescence enhancement level of 46.5-fold, with markedly high reproducibility (coefficient of variation ~ 0.5%) at a FAM concentration of 10 nM. We also found that higher fluorescence enhancement was more likely to be reproducible. This encourages the use of this technology for practical applications in fluorescence-based biochemical assays. Moreover, we investigated a FAM concentration-dependent enhancement of fluorescence. It was found that fluorescence enhancement decreased and saturated at above 10 nM concentration possibly due to partial photo-bleaching of FAM molecules.

scholarly journals Influence of Surface Plasmon Resonance on Fluorescence Emission of Dye-doped Nanoparticles

2016 ◽  
Vol 24 (3S2) ◽  
pp. 121-129
Author(s):  
Chu Viet Ha ◽  
J. C. Brochon ◽  
Tran Hong Nhung
Keyword(s):  
Gold Nanoparticles ◽  
Energy Transfer ◽  
Surface Plasmon ◽  
Fluorescence Enhancement ◽  
Fluorescence Emission ◽  
Fluorescent Nanoparticles ◽  
Dye Molecules ◽  
Recombination Rates ◽  
Surface Plasmon Coupling ◽  
Transfer Mechanisms

The influence of the surface plasmon of gold nanoparticles on the optical properties of the fluorescent nanoparticles in aqueous solution have been investigated. The fluorescence of nanoparticles can be enhanced or quenched in the presence of gold nanoparticles depending on the domination of energy transfer mechanisms: radiating surface plasmon coupling emission or F\"{o}rster energy transfer from fluorescent particles to gold particles, which exciting absorbing plasmon. The fluorescence enhancement or quenching is attributed to the increase or decrease of radiative recombination rates, respectively. The parameters of the energy transfer between fluorescent nanoparticles (dye molecules encapsulated in silica nanoparticles) and nano golds have been estimated. The results show that the interactions between nanoparticles depend on the size of both fluorophores (as donors) and gold nanoparticles (as acceptors).

Effect of Gold Nanoparticles on Fluorescence Properties of Hyperbranched Poly(amido amine)s

2012 ◽  
Vol 441 ◽  
pp. 413-420 ◽  
Author(s):  
Miao Jun Ding ◽  
Yin Wang ◽  
Xin Ke Sun ◽  
Guo Hua Jiang
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon ◽  
Methyl Acrylate ◽  
Michael Addition ◽  
Fluorescence Enhancement ◽  
Excitation Wavelength ◽  
Blue Fluorescence ◽  
Plasmon Absorption ◽  
Practical Applications ◽  
Hyperbranched Poly

Hyperbranched poly (amino amine) s (HPAMAMs), synthesized by Michael addition of 1-(2-aminoethyl) piperazine (AEPZ) and methyl acrylate (MA), can emit blue fluorescence under excitation wavelength. However, the relatively weak fluorescence of HPAMAMs is still an obstacle for its practical applications. Gold is one of the most frequently used metals for fluorescence enhancement. In this research, the influence of gold nanoparticles on fluorescence property of HPAMAMs was investigated by fluorometry. It was found that gold nanoparticles (GNPs) with smaller size (< 5 nm) presented greatly enhanced emission. The GNPs with larger size (~10 nm in diameter) and definite surface plasmon absorption can quench the fluorescence of HPAMAMs.

The Difference in Sensing Properties Towards Anions between Two Pyridinium Amide-Based Receptors with a Subtle Change of H-Bond Position

2020 ◽  
Vol 17 (6) ◽  
pp. 472-478
Author(s):  
Wei-tao Gong ◽  
Wei-dong Qu ◽  
Guiling Ning
Keyword(s):  
Fluorescence Enhancement ◽  
Small Difference ◽  
Fluorescence Emission ◽  
Subtle Change ◽  
Sensing Properties ◽  
Naked Eye ◽  
Recognition Ability ◽  
The Difference ◽  
L1 And L2

Two pyridinium amide-based receptors L1 and L2 with a small difference of H-bond position of the amide have been synthesized and characterized. Interestingly, they exhibited a huge difference in sensing towards AcO- and H2PO4 -, respectively. Receptor L1 was found to be ‘naked-eye’ selective for AcO- anions, while receptor L2 showed clear fluorescence enhancement selective to H2PO4 - anion. The recognition ability has been established by fluorescence emission, UV-vis spectra, and 1HNMR titration.

Chiral surface plasmon-enhanced chiral spectroscopy: principles and applications

Nanoscale ◽  
2021 ◽  
Author(s):  
Xijiao Mu ◽  
Li Hu ◽  
Yuqing Cheng ◽  
Yurui Fang ◽  
Mengtao Sun
Keyword(s):  
Surface Plasmons ◽  
Surface Plasmon ◽  
Scientific Research ◽  
Research Results ◽  
Development Context ◽  
Chiral Surface

In this review, the development context and scientific research results of chiral surface plasmons (SPs) in recent years are classified and described in detail.

Surface plasmon interferometry: measuring group velocity of surface plasmons

2007 ◽  
Vol 32 (10) ◽  
pp. 1235 ◽  
Author(s):  
Vasily V. Temnov ◽  
Ulrike Woggon ◽  
José Dintinger ◽  
Eloise Devaux ◽  
Thomas W. Ebbesen
Keyword(s):  
Group Velocity ◽  
Surface Plasmons ◽  
Surface Plasmon

Pillar[5]arene-based supramolecular assemblies with two-step sequential fluorescence enhancement for mitochondria-targeted cell imaging

2020 ◽  
Vol 8 (44) ◽  
pp. 15622-15625
Author(s):  
Hao Guo ◽  
Xin Yan ◽  
Bing Lu ◽  
Jin Wang ◽  
Xiaolei Yuan ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescence Enhancement ◽  
Fluorescence Emission ◽  
Supramolecular Assemblies ◽  
Live Cell ◽  
Supramolecular Nanoparticles

Two-step sequential fluorescence emission enhanced supramolecular nanoparticles are constructed from pillar[5]arene based host–guest interaction and a linear amphiphile. These supramolecular nanoparticles can be applied in mitochondria-targeted live cell imaging.

Fluorescence Enhancement of Europium(III) Perchlorate by 2,2′-Dipyridyl on Bis(benzylsulfinyl)methane Complex

2013 ◽  
Vol 634-638 ◽  
pp. 2462-2465
Author(s):  
Wen Xian Li ◽  
Bo Yang Ao ◽  
Jing Zhang
Keyword(s):  
Rare Earth ◽  
Ternary Complex ◽  
Fluorescence Enhancement ◽  
Fluorescence Emission ◽  
Europium Complex ◽  
New Method ◽  
Rare Earth Complexes ◽  
Fluorescence Properties ◽  
Binary Complex ◽  
Fluorescence Lifetimes

A novel ligand with double sulfinyl groups, bis(benzylsulfinyl)methane L, was synthesized by a new method. Its novel ternary complex, has been synthesized [using L as the first ligand, and dipyridyl L' as the second ligand]. In order to study the effect of the second ligand on the fluorescence properties of rare-earth sulfoxide complex, a novel binary europium complex has been synthesized. Photoluminescent measurement showed that the first ligand L could efficiently transfer the energy to Eu (III) ions in the complex. Furthermore, the detailed luminescence analyses on the rare earth complexes indicated that the ternary Eu (III) complex manifested stronger fluorescence intensities, longer lifetimes, and higher fluorescence quantum efficiencies than the binary Eu (III) materials. The fluorescence emission intensities and fluorescence lifetimes of the ternary complex enhanced more obviously than the binary complex.

Sensor chip design for increasing surface-plasmon-assisted fluorescence enhancement of the V-trench biosensor

2016 ◽  
Vol 55 (6) ◽  
pp. 067001 ◽  
Author(s):  
Hiroki Ashiba ◽  
Makoto Fujimaki ◽  
Xiaomin Wang ◽  
Koichi Awazu ◽  
Takamasa Tamura ◽  
...  
Keyword(s):  
Surface Plasmon ◽  
Fluorescence Enhancement ◽  
Sensor Chip ◽  
Chip Design

Trends in the development of sensor devices based on surface plasmon resonance (Review)

2021 ◽  
Vol 56 ◽  
pp. 5-26
Author(s):  
A. V. Samoylov ◽  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmons ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Light Wave ◽  
Monochromatic Light ◽  
Dielectric Substrate ◽  
Spr Imaging ◽  
Imaging Sensors ◽  
Polarization Contrast

Trends in the development of modern sensory devices based on surface plasmon resonance (SPR) are considered. The basic principles of construction of SPR sensor are given. For excitation of surface plasmons on the surface of sensitive elements of biosensory, a prism of total internal reflection is used or a dielectric substrate are used. A thin (dozens nm) film of high-conductive metal (mainly gold or silver) is applied to the working surface of the prisms or dielectric substrate. In a typical observation experiment, SPR is measured dependence on the angle of increasing light intensity, reflected by the resonance sensitive surface of the prism (chip). The optical schemes and principles of work of various SPR sensors are considered: - SPR Sensors with angular modulation, which are the most commonly used method based on the corner registration, in which the SPR occurs. The surface of the metal film is irradiated by monochromatic light and scans on a certain range of angles. There is a kind of SPR sensors with angular modulation, in which there is no mechanical scan of the angle of fall. Such sensors are entirely necessary for excitation of PPRs a set of angles is obtained due to a divergent or convergent light beam. - PPR sensors with a wavelength modulation is based on fixing an angle of falling light at a certain value and modulation of the wavelength of the incident light. Excitation of surface plasmons leads to a characteristic failure in the spectrum of reflected radiation. - Phase sensitive SPR sensors in which a change in the phase of the light wave associated with the surface plasma is measured on one corner of the fall and the wavelength of the light wave and is used as the output signal. - SPR imaging sensors in which the Technology of SPR imaging (SPRi) combines the sensitivity of the SPR with spatial image capabilities. The SPRI circuit uses as a fixed angle (as a rule, a slightly left angle of the SPR) and a fixed wavelength to measure changes in the reflection ability (Δ% R) that occur when the curve of the SPR is shifted due to the change in the refractive index above the surface of the sensor element. - SPR imaging sensors polarization contrast. In order to improve the quality of high-performance SPR imaging sensors in terms of sensitivity and resolution, the method of polarization contrast is used Disadvantages and advantages of SPR sensors are constructed with different principles are considered. The design and prospect of the use of achromatic and suburchast wave plates in the PPR imaging sensors with polarization contrast are considered.

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