scholarly journals A Y-Shaped Microfluidic Device to Study the Combined Effect of Wall Shear Stress and ATP Signals on Intracellular Calcium Dynamics in Vascular Endothelial Cells

Micromachines ◽  
2016 ◽  
Vol 7 (11) ◽  
pp. 213 ◽  
Author(s):  
Zong-Zheng Chen ◽  
Zheng-Ming Gao ◽  
De-Pei Zeng ◽  
Bo Liu ◽  
Yong Luan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Wall Shear Stress ◽  
Intracellular Calcium ◽  
Microfluidic Device ◽  
Vascular Endothelial Cells ◽  
Combined Effect ◽  
Vascular Endothelial ◽  
Calcium Dynamics ◽  
Intracellular Calcium Dynamics

scholarly journals A Y-Shaped Microfluidic Device to Study the Combined Effect of Wall Shear Stress and ATP Signals on Intracellular Calcium Dynamics in Vascular Endothelial Cells

2016 ◽  
Author(s):  
Zong-Zheng Chen ◽  
Zheng-Ming Gao ◽  
De-Pei Zeng ◽  
Bo Liu ◽  
Yong Luan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Wall Shear Stress ◽  
Intracellular Calcium ◽  
Microfluidic Device ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Micro Channel ◽  
Calcium Dynamics ◽  
Intracellular Calcium Dynamics

The intracellular calcium dynamics in vascular endothelial cells (VECs) in response to wall shear stress (WSS) and/or adenosine triphosphate (ATP) have been commonly regarded as an important factor in regulating VEC function and behavior including proliferation, migration and apoptosis. However, the effects of time-varying ATP signals have been usually neglected in the past investigations in the field of VEC mechanobiology. In order to investigate the combined effects of WSS and dynamic ATP signals on the intracellular calcium dynamic in VECs, a Y-shaped microfluidic device, which can provide the cultured cells on the bottom of its mixing micro-channel with stimuli of WSS signal alone and different combinations of WSS and ATP signals in one single micro-channel, is proposed. Both numerical simulation and experimental studies verify the feasibility of its application. Cellular experimental results also suggest that a combination of WSS and ATP signals rather than a WSS signal alone might play a more significant role in VEC Ca2+ signal transduction induced by blood flow.

20203 Quantitative Evaluations of the Time Effects of Wall Shear Stress Loading on the Vascular Endothelial Cells

2014 ◽  
Vol 2014.20 (0) ◽  
pp. _20203-1_-_20203-2_
Author(s):  
Takahiro OKI ◽  
Takayuki SUZUKI ◽  
Mahito TERASHIMA ◽  
Sota YAMAMOTO ◽  
Marie OSHIMA ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Wall Shear Stress ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Time Effects ◽  
Wall Shear ◽  
Quantitative Evaluations
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