scholarly journals Light-Addressable Electrodeposition of Magnetically-Guided Cells Encapsulated in Alginate Hydrogels for Three-Dimensional Cell Patterning

Micromachines ◽  
2014 ◽  
Vol 5 (4) ◽  
pp. 1173-1187 ◽  
Author(s):  
Shih-Hao Huang ◽  
Hsiao-Tzu Chu ◽  
Yan-Min Liou ◽  
Kuo-Sheng Huang
Keyword(s):  
Three Dimensional ◽  
Cell Patterning ◽  
Alginate Hydrogels ◽  
Dimensional Cell

scholarly journals In Situ Tissue Engineering Using Magnetically Guided Three-Dimensional Cell Patterning

2012 ◽  
Vol 18 (7) ◽  
pp. 496-506 ◽  
Author(s):  
Shawn P. Grogan ◽  
Chantal Pauli ◽  
Peter Chen ◽  
Jiang Du ◽  
Christine B. Chung ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Cell Patterning ◽  
Dimensional Cell ◽  
In Situ Tissue Engineering

scholarly journals New approaches for tissue engineering: three dimensional cell patterning using inkjet technology

2008 ◽  
Vol 28 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Chizuka Henmi ◽  
Makoto Nakamura ◽  
Yuichi Nishiyama ◽  
Kumiko Yamaguchi ◽  
Shuichi Mochizuki ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Cell Patterning ◽  
New Approaches ◽  
Dimensional Cell

Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src

Lab on a Chip ◽  
2009 ◽  
Vol 9 (23) ◽  
pp. 3378 ◽  
Author(s):  
Mina Okochi ◽  
Sho Takano ◽  
Yayoi Isaji ◽  
Takeshi Senga ◽  
Michinari Hamaguchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Magnetic Force ◽  
Three Dimensional ◽  
Cell Patterning ◽  
Invasive Capacity ◽  
Dimensional Cell

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

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