scholarly journals Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry

Micromachines ◽  
2013 ◽  
Vol 4 (4) ◽  
pp. 402-413 ◽  
Author(s):  
Jing Yan ◽  
Wolfgang-Andreas Bauer ◽  
Martin Fischlechner ◽  
Florian Hollfelder ◽  
Clemens Kaminski ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Double Emulsion ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Emulsion Droplets ◽  
Oil In Water ◽  
Double Emulsion Droplets

scholarly journals Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2062-2074 ◽  
Author(s):  
Kara K. Brower ◽  
Catherine Carswell-Crumpton ◽  
Sandy Klemm ◽  
Bianca Cruz ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
High Throughput ◽  
Double Emulsion ◽  
Single Droplet ◽  
Emulsion Droplets ◽  
Acid Recovery ◽  
Emulsion Flow ◽  
Double Emulsion Droplets

We have developed a novel workflow (sdDE-FACS, s̲ingle d̲roplet D̲ouble E̲mulsion FACS) that allows robust production, screening, and sorting of single double emulsion droplets with complete nucleic acid recovery.

Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry

2009 ◽  
Vol 75A (5) ◽  
pp. 440-449 ◽  
Author(s):  
Daniel F. Gilbert ◽  
John C. Wilson ◽  
Virginia Nink ◽  
Joseph W. Lynch ◽  
Geoffrey W. Osborne
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Glycine Receptor ◽  
Receptor Function ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Viable Cells

scholarly journals Dielectrophoresis Response of Water-in-Oil-in-Water Double Emulsion Droplets with Singular or Dual Cores

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1121
Author(s):  
Tianyi Jiang ◽  
Yankai Jia ◽  
Haizhen Sun ◽  
Xiaokang Deng ◽  
Dewei Tang ◽  
...  
Keyword(s):  
Electric Fields ◽  
Strong Field ◽  
Microfluidic Channel ◽  
Double Emulsion ◽  
Multiple Emulsion ◽  
Field Frequency ◽  
Emulsion Droplets ◽  
Oil In Water ◽  
Wide Range ◽  
Double Emulsion Droplets

Microfluidic technologies have enabled generation of exquisite multiple emulsion droplets, which have been used in many fields, including single-cell assays, micro-sized chemical reactions, and material syntheses. Electrical controlling is an important technique for droplet manipulation in microfluidic systems, but the dielectrophoretic behaviors of multiple emulsion droplets in electrical fields are rarely studied. Here, we report on the dielectrophoresis response of double emulsion droplets in AC electric fields in microfluidic channel. A core-shell model is utilized for analyzing the polarization of droplet interfaces and the overall dielectrophoresis (DEP) force. The water-in-oil-in-water droplets, generated by glass capillary devices, experience negative DEP at low field frequency. At high frequency, however, the polarity of DEP is tunable by adjusting droplet shell thickness or core conductivity. Then, the behavior of droplets with two inner cores is investigated, where the droplets undergo rotation before being repelled or attracted by the strong field area. This work should benefit a wide range of applications that require manipulation of double emulsion droplets by electric fields.

scholarly journals WebFlow: A Software Package for High-Throughput Analysis of Flow Cytometry Data

2009 ◽  
Vol 7 (1) ◽  
pp. 44-55 ◽  
Author(s):  
Mark M. Hammer ◽  
Nikesh Kotecha ◽  
Jonathan M. Irish ◽  
Garry P. Nolan ◽  
Peter O. Krutzik
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Software Package ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Flow Cytometry Data

scholarly journals A multiplexed cell-free assay to screen for antimicrobial peptides in double emulsion droplets

2021 ◽  
Author(s):  
Nicola Nuti ◽  
Philipp Rottmann ◽  
Ariane Stucki ◽  
Philipp Koch ◽  
Sven Panke ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
High Frequency ◽  
Bacterial Resistance ◽  
Double Emulsion ◽  
Microfluidic System ◽  
Promising Candidate ◽  
Emulsion Droplets ◽  
Oil In Water ◽  
Double Emulsion Droplets ◽  
Automated Methods

The global surge in bacterial resistance against traditional antibiotics triggered intensive research for novel compounds, with antimicrobial peptides (AMPs) identified as a promising candidate. Automated methods to systematically generate and screen AMPs according to their membrane preference, however, are still lacking. We introduce a novel microfluidic system for the simultaneous cell-free production and screening of AMPs for their membrane specificity. On our device, AMPs are cell-free produced within water-in-oil-in-water double emulsion droplets, generated at high frequency. Within each droplet, the peptides can interact with different classes of co-encapsulated liposomes, generating a membrane-specific fluorescent signal. The double emulsions can be incubated and observed in a hydrodynamic trapping array or analysed via flow cytometry. Our approach provides a valuable tool for the discovery and development of membrane-active antimicrobials.

Electrically controlled rapid release of actives encapsulated in double-emulsion droplets

Lab on a Chip ◽  
2018 ◽  
Vol 18 (7) ◽  
pp. 1121-1129 ◽  
Author(s):  
Yankai Jia ◽  
Yukun Ren ◽  
Likai Hou ◽  
Weiyu Liu ◽  
Tianyi Jiang ◽  
...  
Keyword(s):  
Electric Fields ◽  
Double Emulsion ◽  
On Demand ◽  
Emulsion Droplets ◽  
Rapid Release ◽  
Oil In Water ◽  
Double Emulsion Droplets

We utilize electric fields to trigger the on-demand release of different cargos that are encapsulated in water-in-oil-in-water (W/O/W) double-emulsion droplets.

scholarly journals Optimized double emulsion flow cytometry with high-throughput single droplet isolation

2019 ◽  
Author(s):  
Kara K. Brower ◽  
Catherine Carswell-Crumpton ◽  
Sandy Klemm ◽  
Bianca Cruz ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
High Throughput ◽  
Single Cell Analysis ◽  
Double Emulsion ◽  
Droplet Microfluidics ◽  
Cell Analysis ◽  
Single Droplet ◽  
Emulsion Droplets ◽  
Droplet Sorting

Droplet microfluidics has made large impacts in diverse areas such as enzyme evolution, chemical product screening, polymer engineering, and single-cell analysis. However, while droplet reactions have become increasingly sophisticated, phenotyping droplets by a fluorescent signal and sorting them to isolate variants-of-interest remains a field-wide bottleneck. Here, we present an optimized double emulsion workflow, sdDE-FACS, that enables high-throughput phenotyping, selection, and sorting of droplets using standard flow cytometers. Using a 130 μm nozzle, we demonstrate robust post-sort recovery of intact droplets, with little to no shear-induced droplet breakage, at high sort frequency (12-14 kHz) across two industry-standard FACS instruments. We report the first quantitative plate statistics for double emulsion droplet isolation and demonstrate single droplet recovery with >70% efficiency. In addition, we establish complete downstream recovery of nucleic acids from single, sorted double emulsion droplets, an advance in droplet sorting comparable with the capabilities of single-cell FACS. This work resolves several hurdles in the field of high-throughput droplet analysis and paves the way for a variety of new droplet assays, including rare variant isolation and multiparameter single-cell analysis, marrying the full power of flow cytometry with droplet microfluidics.

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