scholarly journals Margination of Platelet-Sized Particles in the Red Blood Cell Suspension Flow through Square Microchannels

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1175
Author(s):  
Masako Sugihara-Seki ◽  
Nozomi Takinouchi
Keyword(s):  
Blood Cell ◽  
Cell Suspension ◽  
Cross Section ◽  
Red Blood Cell ◽  
Laser Scanning ◽  
Confocal Laser ◽  
Cross Sectional ◽  
Flow Through ◽  
The Cross

In the blood flow through microvessels, platelets show high concentrations near the vessel wall. This phenomenon is called margination of platelets and is closely associated with hemostasis and thrombosis. In the present study, we conducted in vitro experiments using platelet-sized fluorescent particles as platelet substitutes to investigate the cross-sectional distribution of these particles in the red blood cell suspension flowing through microchannels with a square cross section. Fluorescence observations were performed to measure the transverse distribution of particles at various heights from the bottom face with the use of a confocal laser scanning microscope system. In downstream cross sections of the channel, particles showed focusing near the four corners rather than uniform margination along the entire circumference of the cross section. The focusing of particles near the corners was more enhanced for higher hematocrits. On the other hand, particles in circular channel flows showed nearly axisymmetric uniform accumulation adjacent to the channel wall. The present result suggests that the segregation of suspended particles in the flow of multicomponent suspensions could have such heterogeneous 2D features of particle distribution in the cross section of channels, especially for rectangular channels often used in microfluidics.

scholarly journals A Flexible Architecture for Extracting Metro Tunnel Cross Sections from Terrestrial Laser Scanning Point Clouds

2019 ◽  
Vol 11 (3) ◽  
pp. 297 ◽  
Author(s):  
Zhen Cao ◽  
Dong Chen ◽  
Yufeng Shi ◽  
Zhenxin Zhang ◽  
Fengxiang Jin ◽  
...  
Keyword(s):  
Cross Section ◽  
Cross Sections ◽  
Laser Scanning ◽  
Terrestrial Laser Scanning ◽  
Point Clouds ◽  
Central Axis ◽  
Cross Sectional ◽  
Linear And Nonlinear ◽  
Metro Tunnel ◽  
The Cross

This paper presents a novel framework to extract metro tunnel cross sections (profiles) from Terrestrial Laser Scanning point clouds. The entire framework consists of two steps: tunnel central axis extraction and cross section determination. In tunnel central extraction, we propose a slice-based method to obtain an initial central axis, which is further divided into linear and nonlinear circular segments by an enhanced Random Sample Consensus (RANSAC) tunnel axis segmentation algorithm. This algorithm transforms the problem of hybrid linear and nonlinear segment extraction into a sole segmentation of linear elements defined at the tangent space rather than raw data space, significantly simplifying the tunnel axis segmentation. The extracted axis segments are then provided as input to the step of the cross section determination which generates the coarse cross-sectional points by intersecting a series of straight lines that rotate orthogonally around the tunnel axis with their local fitted quadric surface, i.e., cylindrical surface. These generated profile points are further refined and densified via solving a constrained nonlinear least squares problem. Our experiments on Nanjing metro tunnel show that the cross sectional fitting error is only 1.69 mm. Compared with the designed radius of the metro tunnel, the RMSE (Root Mean Square Error) of extracted cross sections’ radii only keeps 1.60 mm. We also test our algorithm on another metro tunnel in Shanghai, and the results show that the RMSE of radii only keeps 4.60 mm which is superior to a state-of-the-art method of 6.00 mm. Apart from the accurate geometry, our approach can maintain the correct topology among cross sections, thereby guaranteeing the production of geometric tunnel model without crack defects. Moreover, we prove that our algorithm is insensitive to the missing data and point density.

scholarly journals Effect of inertial lift on a spherical particle suspended in flow through a curved duct

2019 ◽  
Vol 875 ◽  
pp. 1-43 ◽  
Author(s):  
Brendan Harding ◽  
Yvonne M. Stokes ◽  
Andrea L. Bertozzi
Keyword(s):  
Reynolds Number ◽  
Spherical Particle ◽  
Cross Section ◽  
Bend Radius ◽  
Cross Sectional ◽  
Particle Reynolds Number ◽  
Curved Duct ◽  
Sectional Plane ◽  
Flow Through ◽  
The Cross

We develop a model of the forces on a spherical particle suspended in flow through a curved duct under the assumption that the particle Reynolds number is small. This extends an asymptotic model of inertial lift force previously developed to study inertial migration in straight ducts. Of particular interest is the existence and location of stable equilibria within the cross-sectional plane towards which particles migrate. The Navier–Stokes equations determine the hydrodynamic forces acting on a particle. A leading-order model of the forces within the cross-sectional plane is obtained through the use of a rotating coordinate system and a perturbation expansion in the particle Reynolds number of the disturbance flow. We predict the behaviour of neutrally buoyant particles at low flow rates and examine the variation in focusing position with respect to particle size and bend radius, independent of the flow rate. In this regime, the lateral focusing position of particles approximately collapses with respect to a dimensionless parameter dependent on three length scales: specifically, the particle radius, duct height and duct bend radius. Additionally, a trapezoidal-shaped cross-section is considered in order to demonstrate how changes in the cross-section design influence the dynamics of particles.

scholarly journals Tunnel Point Cloud and BIM Model Integration for Cross-section Monitoring

2020 ◽  
Author(s):  
Wensheng Zhang ◽  
Ziqi Hao ◽  
Dong Guo ◽  
Yingkai Gao ◽  
Jack Jianguo Wang
Keyword(s):  
Cross Section ◽  
Point Cloud ◽  
Laser Scanning ◽  
Cloud Model ◽  
Spatial Relationship ◽  
Tangent Vector ◽  
Central Line ◽  
Model Integration ◽  
Cross Sectional ◽  
The Cross

This paper introduces a method for tunnel point cloud and BIM model integration and cross-section monitoring, providing information to analyse tunnel cross-sections and surrounding rock deformation, and support for tunnel maintenance and reconstruction. Three types of data are processed for the integration: laser scanning point cloud, BIM tunnel model and terrain model from oblique photogrammetry. An adaptive BIM modelling scheme is proposed for tunnels with alien structures. Precise spatial registration of the data sets is conducted by applying singular value decomposition (SVD) algorithm to calculate transformation parameters from the point cloud model to the BIM model. Since the tunnel central line has high-order derivability, a cross-section calculation method based on tangent vector is proposed to obtain the cross-sectional profile of tunnels at any mileage. The proposed method has been verified by applying it to a tunnel reconstruction project. The experiment results show that the tunnel point cloud and the BIM model were highly coincident after the integration. The developed program can effectively get the cross-section of the tunnel at any mileage, and correctly express the spatial relationship between the BIM tunnel, the point cloud of tunnel and the external mountainous terrain.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Investigation of the Free and Total Thyroxine-binding Capacity of Plasma Proteins in Thyroid Disorders

1971 ◽  
Vol 10 (04) ◽  
pp. 299-304
Author(s):  
József Takó ◽  
János Fischer ◽  
Jusztina Juhász ◽  
Ilona Sztraka ◽  
István Kapus ◽  
...  
Keyword(s):  
Blood Cell ◽  
Thyroid Function ◽  
Oral Contraceptives ◽  
Red Blood Cell ◽  
Plasma Proteins ◽  
Binding Capacity ◽  
Thyroid Disorders ◽  
Thyroid Function Tests ◽  
Total Thyroxine

SummaryThe results of thyroid function tests have been compared with data on the thyroxine-binding capacity of plasma proteins in hyper-, hypo- and euthyroid cases, the latter including women taking oral contraceptives (Infecundin). It was found that there exists a significant correlation of exponential nature between the in vitro red blood cell 125I-triiodothyronine uptake (RCU) and the free thyroxine-binding capacity of the thyroxine-inding globulin (TBG).

Automated Diagonal Slice and View Solution for 3D Device Structure Analysis

2018 ◽  
Author(s):  
Sang Hoon Lee ◽  
Jeff Blackwood ◽  
Stacey Stone ◽  
Michael Schmidt ◽  
Mark Williamson ◽  
...  
Keyword(s):  
Cross Section ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Image Data ◽  
Planar Surface ◽  
Device Structure ◽  
Cross Sectional ◽  
Device Structures ◽  
The Cross ◽  
Planar Analysis

Abstract The cross-sectional and planar analysis of current generation 3D device structures can be analyzed using a single Focused Ion Beam (FIB) mill. This is achieved using a diagonal milling technique that exposes a multilayer planar surface as well as the cross-section. this provides image data allowing for an efficient method to monitor the fabrication process and find device design errors. This process saves tremendous sample-to-data time, decreasing it from days to hours while still providing precise defect and structure data.

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