scholarly journals Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1109
Author(s):  
Puneet Jain ◽  
Takuya Aida ◽  
Masahiro Motosuke
Keyword(s):  
Fluorescence Anisotropy ◽  
Calibration Procedure ◽  
Ammonia Solution ◽  
Temperature Sensing ◽  
Molecular Probe ◽  
Working Fluid ◽  
Large Temperature ◽  
Temperature Imaging ◽  
Uniform Liquid ◽  
Selection Of

Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol–aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in a microfluidic field with non-uniform liquid thickness. Viscosity dependence and temperature dependence on the anisotropy are also clarified; the results indicate an appropriate selection of relation between molecule size and viscosity is important to obtain a large temperature coefficient in anisotropy. Furthermore, a practical calibration procedure of the apparatus constant is proposed. In addition, the potential of temperature imaging is confirmed by the measurement of temperature distribution under focused laser heating.

scholarly journals The use of organic zeotropic mixture with high temperature glide as a working fluid in medium-temperature vapor power plant

2017 ◽  
Vol 21 (2) ◽  
pp. 1153-1160 ◽  
Author(s):  
Aleksandra Borsukiewicz
Keyword(s):  
Power Plant ◽  
Power Plants ◽  
Electrical Energy ◽  
Hot Water ◽  
Working Fluid ◽  
Large Temperature ◽  
Medium Temperature ◽  
Calculation Results ◽  
Zeotropic Mixture ◽  
Selection Of

The paper presents the idea of using organic substances as working fluids in vapor power plants, in order to convert the low and medium temperature thermal energy sources into electrical energy. The calculation results of the power plant efficiency for butane-ethane zeotropic mixtures of different mass compositions, for the power plant supplied with hot water having a temperature of 120?C. Based on the results of thermal-flow calculations it was found that the use of zeotropic mixture does not allow to increase the efficiency and output of the power plant (these values appeared as slightly lower ones). However, it was found that, through the selection of a mixture of sufficiently large temperature glide, the heat exchange surface of the condenser can be reduced or a co-generation system can be implemented.

Fluorescence Anisotropy Studies of Fluorescein

2021 ◽  
Author(s):  
Puneet Jain ◽  
Masahiro Motosuke ◽  
Takuya Aida
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescence Anisotropy ◽  
Temperature Sensing ◽  
Working Fluid ◽  
Temperature Probe ◽  
Folding State

Fluorescein anisotropy, which is a widely used technique to study the folding state of proteins or affinity of ligands, is used in the present work to study the temperature sensing of fluid in a microchannel, by adding fluorophore in the fluid. Fluorescein was used as a temperature probe, while glycerol-aq. ammonia was used as a working fluid. Fluorescence anisotropy of fluorescein were measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed.

scholarly journals Identification of Existing Challenges and Future Trends for the Utilization of Ammonia-Water Absorption–Compression Heat Pumps at High Temperature Operation

2021 ◽  
Vol 11 (10) ◽  
pp. 4635
Author(s):  
Marcel Ulrich Ahrens ◽  
Maximilian Loth ◽  
Ignat Tolstorebrov ◽  
Armin Hafner ◽  
Stephan Kabelac ◽  
...  
Keyword(s):  
High Temperature ◽  
Water Absorption ◽  
Industrial Sector ◽  
Heat Pumps ◽  
Working Fluid ◽  
Large Temperature ◽  
Future Trends ◽  
Ammonia Water ◽  
Starting Point ◽  
High Temperature Operation

Decarbonization of the industrial sector is one of the most important keys to reducing global warming. Energy demands and associated emissions in the industrial sector are continuously increasing. The utilization of high temperature heat pumps (HTHPs) operating with natural fluids presents an environmentally friendly solution with great potential to increase energy efficiency and reduce emissions in industrial processes. Ammonia-water absorption–compression heat pumps (ACHPs) combine the technologies of an absorption and vapor compression heat pump using a zeotropic mixture of ammonia and water as working fluid. The given characteristics, such as the ability to achieve high sink temperatures with comparably large temperature lifts and high coefficient of performance (COP) make the ACHP interesting for utilization in various industrial high temperature applications. This work reviews the state of technology and identifies existing challenges based on conducted experimental investigations. In this context, 23 references with capacities ranging from 1.4 kW to 4500 kW are evaluated, achieving sink outlet temperatures from 45 °C to 115 °C and COPs from 1.4 to 11.3. Existing challenges are identified for the compressor concerning discharge temperature and lubrication, for the absorber and desorber design for operation and liquid–vapor mixing and distribution and the choice of solution pump. Recent developments and promising solutions are then highlighted and presented in a comprehensive overview. Finally, future trends for further studies are discussed. The purpose of this study is to serve as a starting point for further research by connecting theoretical approaches, possible solutions and experimental results as a resource for further developments of ammonia-water ACHP systems at high temperature operation.

Calculation of a closed hydraulic volumetric system

2021 ◽  
pp. 27-30
Author(s):  
Keyword(s):  
Power Plant ◽  
Wind Power ◽  
Hydraulic System ◽  
Working Fluid ◽  
Wind Power Plant ◽  
Hydraulic Systems ◽  
Hydraulic Motor ◽  
Hydraulic Pump ◽  
Oil Pump ◽  
Selection Of

An algorithm is proposed for calculating a closed volumetric hydraulic pump-hydraulic motor system using the example of the hydraulic system of a wind power plant, based on the calculation of the hydraulic systems of mobile machines. The main characteristics of the system components, the selection of initial data for the calculation, working fluid and diameters of hydraulic lines are analyzed. Keywords: hydraulic system, energy, fluid, oil, pump, motor, renewable energy source, wind power plant, machine. [email protected]

Experimental Study on Forced Convection Heat Transfer Inside Horizontal Tubes in an Absorption/Compression Heat Pump

2002 ◽  
Vol 124 (5) ◽  
pp. 975-978 ◽  
Author(s):  
Li Yong and ◽  
K. Sumathy
Keyword(s):  
Heat Transfer ◽  
Pressure Drop ◽  
Forced Convection ◽  
Experimental Results ◽  
Working Fluid ◽  
Large Temperature ◽  
Absorption Process ◽  
Transfer Coefficients ◽  
Tube Heat Exchanger ◽  
Heat Transfer Coefficients

Quasi-local absorption heat transfer coefficients and pressure drop inside a horizontal tube absorber have been investigated experimentally, with R-22/DMA as the working pair. The absorber is a counterflow coaxial tube-in-tube heat-exchanger with the working fluid flowing in the inner tube while the water moves through the annulus. A large temperature gliding has been experienced during the absorption process. Experimental results show that the heat transfer coefficient of the forced convective vapor absorption process is higher compared to the vertical falling film absorption. A qualitative study is made to analyze the effect of mass flux, vapor quality and solution concentration on pressure drop and heat transfer coefficients. On the basis of the experimental results, a new correlation is proposed whereby the two-phase heat transfer is taken as a product of the forced convection of the absorption and the combined effect of heat and mass transfer at the interface. The correlation is found to predict the experimental data almost within 30 percent.

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