scholarly journals Synthesis of Hyaluronic Acid-Conjugated Fe3O4@CeO2 Composite Nanoparticles for a Target-Oriented Multifunctional Drug Delivery System

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1018
Author(s):  
Chang Ryong Lee ◽  
Gun Gyun Kim ◽  
Sung Bum Park ◽  
Sang Wook Kim
Keyword(s):  
Drug Delivery ◽  
Magnetic Properties ◽  
Hyaluronic Acid ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
X Ray ◽  
Ft Ir

This study is based on the principle that superparamagnetic iron oxide nanoparticles (Fe3O4) can be used to target a specific area given that their magnetic properties emerge when an external magnetic field is applied. Cerium oxide (CeO2), which causes oxidative stress by generating reactive oxygen species (ROS) in the environment of tumor cells, was synthesized on the surface of superparamagnetic iron oxide nanoparticles to produce nanoparticles that selectively kill cancer cells. In addition, hyaluronic acid (HA) was coated on the cerium’s surface to target CD44-overexpressing tumor cells, and natZr was chelated on the Fe3O4@CeO2 surface to show the usefulness of labeling the radioisotope 89Zr (T1/2 = 3.3 d). The synthesis of Fe3O4@CeO2 was confirmed by Fourier Transform-Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD) and Field Emission-Transmission Electron Microscope (FE-TEM). The coating of HA was confirmed by FT-IR, X-ray Photoelectron. Spectroscopy (XPS), FE-TEM, Energy-Dispersive X-ray Spectroscopy (EDS) and Thermogravimetric Analysis (TGA)/Differential Scanning Calorimetry (DSC). The sizes of the prepared nanoparticles were confirmed through FE-TEM and Field Emission-Scanning Electron (FE-SEM) (sizes of 15 to 30 nm), and it was confirmed that natZr was introduced onto the surface of the nanoparticles using EDS. The particle size of the dispersed material was limited through Dynamic Light Scattering (DLS) to about 148 nm in aqueous solution, which was suitable for the (enhanced permeation and retention) EPR effect. It was confirmed that the HA-coated nanoparticles have good dispersibility. Finally, a cytotoxicity evaluation confirmed the ability of CeO2 to generate ROS and target the delivery of HA. In conclusion, Fe3O4@CeO2 can effectively inhibit cancer cells through the activity of cerium oxide in the body when synthesized in nano-sized superparamagnetic coral iron that has magnetic properties. Subsequently, by labeling the radioactive isotope 89Zr, it is possible to create a theranostic drug delivery system that can be used for cancer diagnosis.

scholarly journals Reversible magnetism switching of iron oxide nanoparticle dispersions by controlled agglomeration

2021 ◽  
Author(s):  
Stephan Müssig ◽  
Björn Kuttich ◽  
Florian Fidler ◽  
Daniel Haddad ◽  
Susanne Wintzheimer ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Iron Oxide ◽  
Light Scattering ◽  
Superparamagnetic Iron Oxide ◽  
Iron Oxide Nanoparticle ◽  
Oxide Nanoparticles ◽  
X Ray ◽  
X Ray Scattering ◽  
Nanoparticle Dispersions ◽  
Oxide Nanoparticle

The controlled agglomeration of superparamagnetic iron oxide nanoparticles (SPIONs) was used to rapidly switch their magnetic properties. Small-angle X-ray scattering (SAXS) and dynamic light scattering showed that tailored iron oxide...

scholarly journals Lipid Cubic Mesophases Combined with Superparamagnetic Iron Oxide Nanoparticles: A Hybrid Multifunctional Platform with Tunable Magnetic Properties for Nanomedical Applications

2021 ◽  
Vol 22 (17) ◽  
pp. 9268
Author(s):  
Lucrezia Caselli ◽  
Marco Mendozza ◽  
Beatrice Muzzi ◽  
Alessandra Toti ◽  
Costanza Montis ◽  
...  
Keyword(s):  
Phase Transition ◽  
Drug Delivery ◽  
Magnetic Properties ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Energy ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Bulk Liquid ◽  
Oxide Nanoparticles

Hybrid materials composed of superparamagnetic iron oxide nanoparticles (SPIONs) and lipid self-assemblies possess considerable applicative potential in the biomedical field, specifically, for drug/nutrient delivery. Recently, we showed that SPIONs-doped lipid cubic liquid crystals undergo a cubic-to-hexagonal phase transition under the action of temperature or of an alternating magnetic field (AMF). This transition triggers the release of drugs embedded in the lipid scaffold or in the water channels. In this contribution, we address this phenomenon in depth, to fully elucidate the structural details and optimize the design of hybrid multifunctional carriers for drug delivery. Combining small-angle X-ray scattering (SAXS) with a magnetic characterization, we find that, in bulk lipid cubic phases, the cubic-to-hexagonal transition determines the magnetic response of SPIONs. We then extend the investigation from bulk liquid-crystalline phases to colloidal dispersions, i.e., to lipid/SPIONs nanoparticles with cubic internal structure (“magnetocubosomes”). Through Synchrotron SAXS, we monitor the structural response of magnetocubosomes while exposed to an AMF: the magnetic energy, converted into heat by SPIONs, activates the cubic-to-hexagonal transition, and can thus be used as a remote stimulus to spike drug release “on-demand”. In addition, we show that the AMF-induced phase transition in magnetocubosomes steers the realignment of SPIONs into linear string assemblies and connect this effect with the change in their magnetic properties, observed at the bulk level. Finally, we assess the internalization ability and cytotoxicity of magnetocubosomes in vitro on HT29 adenocarcinoma cancer cells, in order to test the applicability of these smart carriers in drug delivery applications.

Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

2020 ◽  
Vol 13 ◽  
Author(s):  
Selin Yılmaz ◽  
Çiğdem İçhedef ◽  
Kadriye Buşra Karatay ◽  
Serap Teksöz
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Cancer Cells ◽  
Cancer Drug ◽  
Oxide Nanoparticles ◽  
Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

MAGNETIC BEHAVIOR OF Ni-DOPED CuO NANOPARTICLES SYNTHESIZED BY MICROWAVE IRRADIATION METHOD

2019 ◽  
Vol 26 (05) ◽  
pp. 1850184 ◽  
Author(s):  
C. THANGAMANI ◽  
M. PONNAR ◽  
P. PRIYADHARSHINI ◽  
P. MONISHA ◽  
S. S. GOMATHI ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Microwave Irradiation ◽  
Copper Oxide ◽  
Lattice Constant ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Microwave Irradiation Method ◽  
Nickel Doping ◽  
X Ray ◽  
Ft Ir

Nickel-substituted copper oxide nanoparticles at various concentrations were synthesized by the microwave irradiation technique. The consequence of nickel doping on crystal structure, optical properties, and magnetic properties was examined by means of X-ray diffractometer, ultraviolet-visible spectrometer, Fourier transform infrared (FT-IR) spectrometer, transmission electron microscope, and vibrating sample magnetometer (VSM). X-ray diffraction analysis shows that the samples are monoclinic and their crystallite size varies from 25[Formula: see text]nm to 42[Formula: see text]nm, and lattice constant significantly increases with nickel concentration. Additional increase of nickel content (7%) decreases the lattice constant. TEM micrograph witnessed that the prepared nanoparticles were sphere-shaped and the particle distribution is in the range between 20 and 40[Formula: see text]nm. Bandgap measurement reveals that both undoped and nickel-doped copper oxides are direct bandgap semiconductor materials with bandgaps of 3.21 and 3.10[Formula: see text]eV, respectively, FT-IR spectra of the synthesized samples confirmed the nickel doping. VSM studies confirmed the ferromagnetic behavior of the synthesized samples at room temperature. The results revealed that the nickel-doped copper oxide nanoparticles synthesized via the microwave irradiation method exhibit better magnetic properties than the undoped copper oxide.

Superparamagnetic iron oxide nanoparticles enhance glioma radiosensitivity via inducing cell cycle arrest and apoptosis

2020 ◽  
Author(s):  
Jinning Mao ◽  
Meng Jiang ◽  
Xingliang Dai ◽  
Guodong Liu ◽  
Zhixiang Zhuang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Glioma Cells ◽  
Superparamagnetic Iron Oxide ◽  
Spherical Shape ◽  
Treated Group ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
X Ray

Abstract Aim: Superparamagnetic iron oxide nanoparticles (SPIONs) is a widely used biomedical material for imaging and targeting drug delivery. We synthesized SPIONs and tested their effects on the radiosensitization of glioma.Methods: Acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (Fe3O4 NPs) were synthesized via a one-step hydrothermal approach and the surface was chemically modified with acetic anhydride to generate surface charge-neutralized NPs. NPs were characterized by TEM and ICP-AES. Radiosensitivity of U87MG glioma cells was evaluated by MTT assay. Cell cycle and apoptosis in glioma cells were examined by flow cytometry. Results: APTS-coated Fe3O4 NPs had a spherical or quasi-spherical shape with average size of 10.5±1.1 nm. NPs had excellent biocompatibility and intracellular uptake of NPs reached the peak 24 hours after treatment. U87 cell viability decreased significantly after treatment with both X-ray and NPs compared to X-ray treatment alone. Compared to X-ray treatment alone, the percentage of cells in G2/M phase (31.83%) significantly increased in APTS-coated Fe3O4 NPs plus X-ray treated group (P<0.05). In addition, the percentage of apoptotic cells was significant higher in APTS-coated Fe3O4 NPs plus X-ray treated group than in X-ray treatment alone group (P<0.05). Conclusion: APTS-coated Fe3O4 NPs achieved excellent biocompatibility and increased radiosensitivity for glioma cells.

scholarly journals A Composite of Hyaluronic Acid-Modified Graphene Oxide and Iron Oxide Nanoparticles for Targeted Drug Delivery and Magnetothermal Therapy

ACS Omega ◽  
2019 ◽  
Vol 4 (5) ◽  
pp. 9284-9293 ◽  
Author(s):  
Nilkamal Pramanik ◽  
Santhalakshmi Ranganathan ◽  
Sunaina Rao ◽  
Kaushik Suneet ◽  
Shilpee Jain ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Graphene Oxide ◽  
Hyaluronic Acid ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Targeted Drug Delivery ◽  
Oxide Nanoparticles ◽  
Modified Graphene Oxide ◽  
Targeted Drug ◽  
Modified Graphene
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