scholarly journals Simultaneous Detection of Viability and Concentration of Microalgae Cells Based on Chlorophyll Fluorescence and Bright Field Dual Imaging

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 896
Author(s):  
Yanjuan Wang ◽  
Junsheng Wang ◽  
Tianqi Wang ◽  
Chengxiao Wang
Keyword(s):  
Chlorophyll Fluorescence ◽  
Ballast Water ◽  
Low Cost ◽  
Simultaneous Detection ◽  
Prototype System ◽  
Detection Accuracy ◽  
High Concentration ◽  
Bright Field ◽  
Microscopy Imaging ◽  
Ship Ballast Water

Ship ballast water contains high concentration of plankton, bacteria, and other microorganisms. If the huge amount of ballast water is discharged without being inactivated, it will definitely spell disaster to the marine environment. Microalgae is the most common species exiting in ballast water, so the detection of the concentration and viability of microalgae is a very important issue. The traditional methods of detecting microalgae in ballast water were costly and need the help of bulky equipment. Herein, a novel method based on microalgae cell intracellular chlorophyll fluorescence (CF) imaging combines with cell bright field (BF) microscopy was proposed. The geometric features of microalgae cells were obtained by BF image, and the cell viability was obtained by CF image. The two images were fused through the classic image registration algorithm to achieve simultaneous detection of the viability and concentration of microalgae cells. Furthermore, a low-cost, miniaturized CF/BF microscopy imaging prototype system based on the above principles was designed. In order to verify the effectiveness of the proposed method, four typical microalgae in ballast water (Platymonas, Pyramimonas sp., Chrysophyta, and Prorocentrum lima) were selected as the samples. The experimental results show that the self-developed prototype can quickly and accurately determine the concentration and the viability of microalgae cells in ship ballast water based on the dual images of BF and CF, and the detection accuracy is equivalent to that of commercial microscope. It was the first time to simultaneously detect the viability and concentration of microalgae cells in ship ballast water using the method that combining the fluorescence and bright field images; moreover, a miniaturized microscopic imaging prototype was developed. Those findings expected to contribute to the microalgae detection and ship ballast water management.

scholarly journals Enzymatic Low Volume Passive Sweat Based Assays for Multi-Biomarker Detection

Biosensors ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 13 ◽  
Author(s):  
Ashlesha Bhide ◽  
Sarah Cheeran ◽  
Sriram Muthukumar ◽  
Shalini Prasad
Keyword(s):  
Dynamic Range ◽  
Low Cost ◽  
Simultaneous Detection ◽  
Porous Membrane ◽  
Zno Films ◽  
Current Response ◽  
Biomarker Detection ◽  
High Concentration ◽  
Sensor Performance ◽  
Stable Current

Simultaneous detection of correlated multi-biomarkers on a single low-cost platform in ultra-low fluid volumes with robustness is in growing demand for the development of wearable diagnostics. A non-faradaic biosensor for the simultaneous detection of alcohol, glucose, and lactate utilizing low volumes (1–5 μL) of sweat is demonstrated. Biosensing is implemented using nanotextured ZnO films integrated on a flexible porous membrane to achieve enhanced sensor performance. The ZnO sensing region is functionalized with enzymes specific for the detection of alcohol, glucose, and lactate in the ranges encompassing their physiologically relevant levels. A non-faradaic chronoamperometry technique is used to measure the current changes associated with interactions of the target biomarkers with their specific enzyme. The specificity performance of the biosensing platform was established in the presence of cortisol as the non-specific molecule. Biosensing performance of the platform in a continuous mode performed over a 1.5-h duration showed a stable current response to cumulative lifestyle biomarker concentrations with capability to distinguish reliably between low, mid, and high concentration ranges of alcohol (0.1, 25, 100 mg/dL), glucose (0.1, 10, 50 mg/dL), and lactate (1, 50, 100 mM). The low detection limits and a broader dynamic range for the lifestyle biomarker detection are quantified in this research demonstrating its suitability for translation into a wearable device.

scholarly journals An Anti-Counterfeiting Architecture for Traceability System Based on Modified Two-Level Quick Response Codes

Electronics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 320
Author(s):  
Shundao Xie ◽  
Hong-Zhou Tan
Keyword(s):  
Product Life Cycle ◽  
Low Cost ◽  
Prototype System ◽  
Qr Code ◽  
Quick Response ◽  
Copy Detection ◽  
Traceability System ◽  
Scanned Image ◽  
Promising Solution ◽  
Product Package

Traceability is considered a promising solution for product safety. However, the data in the traceability system is only a claim rather than a fact. Therefore, the quality and safety of the product cannot be guaranteed since we cannot ensure the authenticity of products (aka counterfeit detection) in the real world. In this paper, we focus on counterfeit detection for the traceability system. The risk of counterfeiting throughout a typical product life cycle in the supply chain is analyzed, and the corresponding requirements for the tags, packages, and traceability system are given to eliminate these risks. Based on the analysis, an anti-counterfeiting architecture for traceability system based on two-level quick response codes (2LQR codes) is proposed, where the problem of counterfeit detection for a product is transformed into the problem of copy detection for the 2LQR code tag. According to the characteristics of the traceability system, the generation progress of the 2LQR code is modified, and there is a corresponding improved algorithm to estimate the actual location of patterns in the scanned image of the modified 2LQR code tag to improve the performance of copy detection. A prototype system based on the proposed architecture is implemented, where the consumers can perform traceability information queries by scanning the 2LQR code on the product package with any QR code reader. They can also scan the 2LQR code with a home-scanner or office-scanner, and send the scanned image to the system to perform counterfeit detection. Compared with other anti-counterfeiting solutions, the proposed architecture has advantages of low cost, generality, and good performance. Therefore, it is a promising solution to replace the existing anti-counterfeiting system.

Improved photocatalytic conversion of high−concentration ammonia in water by low−cost Cu/TiO2 and its mechanism study

Chemosphere ◽  
2021 ◽  
Vol 274 ◽  
pp. 129689
Author(s):  
Jianpei Feng ◽  
Xiaolei Zhang ◽  
Guan Zhang ◽  
Ji Li ◽  
Wei Song ◽  
...  
Keyword(s):  
Low Cost ◽  
Mechanism Study ◽  
High Concentration

scholarly journals A V-SLAM Guided and Portable System for Photogrammetric Applications

2021 ◽  
Vol 13 (12) ◽  
pp. 2351
Author(s):  
Alessandro Torresani ◽  
Fabio Menna ◽  
Roberto Battisti ◽  
Fabio Remondino
Keyword(s):  
Low Cost ◽  
Cost Effective ◽  
Motion Blur ◽  
Guidance System ◽  
Prototype System ◽  
Active Sensors ◽  
Localization And Mapping ◽  
Acquisition Speed ◽  
Mobile Mapping System ◽  
Slam Algorithm

Mobile and handheld mapping systems are becoming widely used nowadays as fast and cost-effective data acquisition systems for 3D reconstruction purposes. While most of the research and commercial systems are based on active sensors, solutions employing only cameras and photogrammetry are attracting more and more interest due to their significantly minor costs, size and power consumption. In this work we propose an ARM-based, low-cost and lightweight stereo vision mobile mapping system based on a Visual Simultaneous Localization And Mapping (V-SLAM) algorithm. The prototype system, named GuPho (Guided Photogrammetric System) also integrates an in-house guidance system which enables optimized image acquisitions, robust management of the cameras and feedback on positioning and acquisition speed. The presented results show the effectiveness of the developed prototype in mapping large scenarios, enabling motion blur prevention, robust camera exposure control and achieving accurate 3D results.

scholarly journals Synthesis of poly (n-butyl acrylate) with tempo by nitroxide mediated polymerization method

2021 ◽  
pp. 096739112110245
Author(s):  
Amrita Sharma ◽  
PP Pande
Keyword(s):  
Butyl Acrylate ◽  
Low Cost ◽  
Polymerization Process ◽  
Polymerization Reaction ◽  
High Concentration ◽  
Acrylate Monomer ◽  
Controlled Polymerization ◽  
Acrylate Monomers ◽  
Nitroxide Mediated Polymerization ◽  
Polymerization Method

It has been observed that acrylate monomers are very difficult to polymerize with the low cost nitroxide catalyst 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO). Therefore, costly acyclic nitroxides such as N-tert-butyl-N-(1-diethylphosphono-2,2-dimethyl)-N-oxyl, (SG1), 2,2,5-Trimethyl-4-phenyl-3-azahexane-3-nitroxide (TIPNO) and TIPNO derivatives have to be used for the polymerization of the acrylic acid derivatives. There are very few reports on the use of TEMPO-derivatives toward the polymerization of n-butyl acrylate. Generally different reducing agents viz. glucose, ascorbic acid, hydroxyacetone etc. have been used to destroy excess TEMPO during the polymerization reaction. The acrylate polymerizations fail in the presence of TEMPO due to the strong C–O bond formed between the acrylate chain end and nitroxide. To the best of our knowledge, no literature report is available on the use of TEMPO without reducing agent or high temperature initiators, toward the polymerization of n-butyl acrylate. The present study has been carried out with a view to re-examine the application of low cost nitroxide TEMPO, so that it can be utilized towards the polymerization of acrylate monomers (e.g. n-butyl acrylate). We have been able to polymerize n-butyl acrylate using the nitroxide TEMPO as initiator (via a macroinitiator). In this synthesis, a polystyrene macroinitiator was synthesized in the first step from TEMPO, after this TEMPO end-capped styrene macroinitiator (PSt-TEMPO) is used to polymerize n-butyl acrylate monomer. The amount of macroinitiator taken was varied from 0.05% to 50% by weight of n-butyl acrylate monomer. The polymerization was carried out at 120°C by bulk polymerization method. The experimental findings showed a gradual increase in molecular weight of the polymer formed and decrease in the polydispersity index (PDI) with increase in amount of PSt-TEMPO macroinitiator taken. In all experiments conversion was more than 80%. These results indicate that the polymerization takes place through controlled polymerization process. Effect of different solvents on polymerization has also been investigated. In the following experiments TEMPO capped styrene has been used as macroinitiator leading to the successful synthesis of poly n-Butyl acrylate. It has been found that styrene macroinitiator is highly efficient for the nitroxide mediated polymerization, even in very small concentration for the synthesis of poly n-butyl acrylate. High concentration of macroinitiator results in the formation of block copolymers of polystyrene and poly ( n-butyl acrylate) viz. polystyrene-block-poly-( n-butyl acrylate). The use of TEMPO toward controlled polymerization is of much importance, because it is the nitroxide commercially available at the lowest cost.

scholarly journals Annular Bright Field Scanning Transmission Electron Microscopy Imaging Dynamics

2010 ◽  
Vol 16 (S2) ◽  
pp. 80-81 ◽  
Author(s):  
SD Findlay ◽  
N Shibata ◽  
H Sawada ◽  
E Okunishi ◽  
Y Kondo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Scanning Transmission Electron Microscopy ◽  
Bright Field ◽  
Microscopy Imaging ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

Low cost, trouble-free disposable pencil graphite electrode sensor for the simultaneous detection of hydroquinone and catechol

2021 ◽  
pp. 125663
Author(s):  
T.S. Sunil Kumar Naik ◽  
Arul Varman Kesavan ◽  
B.E. Kumara Swamy ◽  
Simranjeet Singh ◽  
Amith G. Anil ◽  
...  
Keyword(s):  
Graphite Electrode ◽  
Low Cost ◽  
Simultaneous Detection ◽  
Pencil Graphite Electrode ◽  
Electrode Sensor

scholarly journals Automated Low Cost Method of Quantification of Hepatitis C Virus

2015 ◽  
Vol 14 (3) ◽  
pp. 247-253
Author(s):  
Faiz MMT Marikar ◽  
Dammika Senevirathna ◽  
Neil Fernandopulle
Keyword(s):  
Viral Load ◽  
Hepatitis C ◽  
Blood Donors ◽  
Low Cost ◽  
Medical Science ◽  
Simultaneous Detection ◽  
Plasma Samples ◽  
Rt Pcr ◽  
Viral Genomes ◽  
Post Therapy

This paper describes the development of a Dig-dUTP based multiplex real time RT-PCR for the simultaneous detection of HCV viral amount in plasma samples. Viral genomes were identified in the same sample by Dig-dUTP PCR 216 bp region. Analysis of known scalar concentrations of reference plasma indicated that the multiplex procedure detects at least 500 copies/ml of HCV. In addition, we also assayed HCV viral load in eighty co-infected patients and in fifteen blood donors, confirming the sensitivity and specificity of the assay. This method may represent a useful alternative method for the detection of HCV co-infection, reliable for a rapid and relatively inexpensive screening of blood donors. The assay may be used to determine post-therapy viral clearance.Bangladesh Journal of Medical Science Vol.14(3) 2015 p.247-253

Development of All-Plastic Microvalve Array for Multiplexed Immunoassay

2014 ◽  
Author(s):  
Shancy Augustine ◽  
Pan Gu ◽  
Xiangjun Zheng ◽  
Toshikazu Nishida ◽  
Z. Hugh Fan
Keyword(s):  
Large Scale ◽  
Low Cost ◽  
Simultaneous Detection ◽  
Cost Effective ◽  
Negative Control ◽  
Sensitive Material ◽  
Detection Scheme ◽  
Power Battery ◽  
Multiple Analytes ◽  
Multiplexed Immunoassay

There is a need for low-cost immunoassays that measure the presence and concentration of multiple harmful agents in one device. Currently, comparable immunoassays employ a one-analyte-per-test format that is time consuming and not cost effective for the requirement of detecting multiple analytes in a single sample. For instance, if a spectrum of harmful agents, including E. coli O157, cholera toxin, and Salmonella typhimurium, should be simultaneously monitored in foods and drinking water, then a one-analyte-per-test would be inefficient. This work demonstrates a platform capable of simultaneous detection of multiple analytes in a single, low-cost, microvalve array-enabled multiplexed immunoassay. This multiplexed immunoassay platform is demonstrated in a prototype COC (cyclic olefin copolymer) device with a 2×3 array in which 6 analytes can be detected simultaneously. In order to contain and regulate the flow of reagents in the multichannel device, an array of microfluidic valves actuated by a thermally expandable material and microfabricated resistors have been developed to direct the flow to the necessary assay sites. The microvalve-based immunoassay is shown to be reliable, easy to operate, and compatible with large-scale integration. The all-plastic microvalves use paraffin wax as the thermally sensitive material which drastically reduces power consumption by latching upon closing so that pulsed power is required only to close and latch the microvalve until it is necessary to re-open the valve. The multiplexed detection scheme has been demonstrated by using three proteins, C reactive protein (CRP) and transferrin, both of which are biomarkers associated with traumatic brain injury (TBI) as well as bovine serum albumin (BSA) as the negative control. Since there are no external bulky pneumatic accessories required to operate/latch the microvalves in the device, this compact, thermally actuated and latching microvalve-enabled multiplexed immunoassay has the potential to realize a portable, low power, battery operated microfluidic device for biological assays.

scholarly journals Non-intrusive vital sign monitoring using an intelligent pillow based on a piezoelectric ceramic sensor

2020 ◽  
Vol 15 ◽  
pp. 155892502097726
Author(s):  
Wei Wang ◽  
Zhiqiang Pang ◽  
Ling Peng ◽  
Fei Hu
Keyword(s):  
Heart Rate ◽  
Real Time ◽  
Piezoelectric Ceramic ◽  
Low Cost ◽  
Body Movement ◽  
Vital Signs ◽  
High Heart Rate ◽  
Detection Accuracy ◽  
Processing Unit ◽  
Real Time Monitoring

Performing real-time monitoring for human vital signs during sleep at home is of vital importance to achieve timely detection and rescue. However, the existing smart equipment for monitoring human vital signs suffers the drawbacks of high complexity, high cost, and intrusiveness, or low accuracy. Thus, it is of great need to develop a simplified, nonintrusive, comfortable and low cost real-time monitoring system during sleep. In this study, a novel intelligent pillow was developed based on a low-cost piezoelectric ceramic sensor. It was manufactured by locating a smart system (consisting of a sensing unit i.e. a piezoelectric ceramic sensor, a data processing unit and a GPRS communication module) in the cavity of the pillow made of shape memory foam. The sampling frequency of the intelligent pillow was set at 1000 Hz to capture the signals more accurately, and vital signs including heart rate, respiratory rate and body movement were derived through series of well established algorithms, which were sent to the user’s app. Validation experimental results demonstrate that high heart-rate detection accuracy (i.e. 99.18%) was achieved in using the intelligent pillow. Besides, human tests were conducted by detecting vital signs of six elder participants at their home, and results showed that the detected vital signs may well predicate their health conditions. In addition, no contact discomfort was reported by the participants. With further studies in terms of validity of the intelligent pillow and large-scale human trials, the proposed intelligent pillow was expected to play an important role in daily sleep monitoring.

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