scholarly journals A New Predictive Technology for Perinatal Stem Cell Isolation Suited for Cell Therapy Approaches

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 782
Author(s):  
Silvia Zia ◽  
Giulia Martini ◽  
Valeria Pizzuti ◽  
Alessia Maggio ◽  
Giuliana Simonazzi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quality Control ◽  
Stem Cell ◽  
New Technology ◽  
Cell Isolation ◽  
Individual Variability ◽  
Label Free ◽  
Differentiation Potential ◽  
Proliferation Ability ◽  
Stem Cell Isolation

The use of stem cells for regenerative applications and immunomodulatory effect is increasing. Amniotic epithelial cells (AECs) possess embryonic-like proliferation ability and multipotent differentiation potential. Despite the simple isolation procedure, inter-individual variability and different isolation steps can cause differences in isolation yield and cell proliferation ability, compromising reproducibility observations among centers and further applications. We investigated the use of a new technology as a diagnostic tool for quality control on stem cell isolation. The instrument label-free separates cells based on their physical characteristics and, thanks to a micro-camera, generates a live fractogram, the fingerprint of the sample. Eight amniotic membranes were processed by trypsin enzymatic treatment and immediately analysed. Two types of profile were generated: a monomodal and a bimodal curve. The first one represented the unsuccessful isolation with all recovered cell not attaching to the plate; while for the second type, the isolation process was successful, but we discovered that only cells in the second peak were alive and resulted adherent. We optimized a Quality Control (QC) method to define the success of AEC isolation using the fractogram generated. This predictive outcome is an interesting tool for laboratories and cell banks that isolate and cryopreserve fetal annex stem cells for research and future clinical applications.

Label-free microfluidic stem cell isolation technologies

2017 ◽  
Vol 89 ◽  
pp. 1-12 ◽  
Author(s):  
Anoop Menachery ◽  
Nityanand Kumawat ◽  
Mohammad Qasaimeh
Keyword(s):  
Stem Cell ◽  
Cell Isolation ◽  
Label Free ◽  
Stem Cell Isolation

scholarly journals Application potential and plasticity of human stem cells

2019 ◽  
Vol 7 (3) ◽  
pp. 140-145 ◽  
Author(s):  
Mikołaj Lorenz ◽  
Paul Mozdziak ◽  
Bartosz Kempisty ◽  
Marta Dyszkiewicz-Konwińska
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Cell Isolation ◽  
Human Stem Cells ◽  
Heart Attacks ◽  
Isolation Methods ◽  
Application Potential ◽  
The Subject ◽  
Stem Cell Isolation

AbstractSignificant advances have been achieved in the study of stem cells over recent years. Stem cell isolation, their plasticity, differentiation and pre-clinical and clinical applications have undergone a significant study. The objective of this paper is to review the advances in stem cell isolation methods. There are many types of stem cells in the article. Isolation and subsequent differentiation of among others: Human adipose-derived stem cells, cancer stem cells, neural stem cells and mesenchymal stem cells. The subject of Endometrial mesenchymal stromal cells, whose isolation methods are relatively new, was also raised. Attention was paid to the development of preclinical studies using Dental Pulp Stem Cells in various diseases such as Parkinson’s disease or Alzheimer’s disease. Progress in research on the use of stem cells in the treatment of heart attacks, burns, bone injuries and the use of neural stem cells in animal models as an attempt to treat multiple sclerosis has been described.Running title: Potential and plasticity of stem cells

Recovery of Stem Cells from Cryopreserved Periodontal Ligament

2005 ◽  
Vol 84 (10) ◽  
pp. 907-912 ◽  
Author(s):  
B.-M. Seo ◽  
M. Miura ◽  
W. Sonoyama ◽  
C. Coppe ◽  
R. Stanyon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Cell Isolation ◽  
Clinical Approach ◽  
Human Tissues ◽  
Periodontal Ligament Stem Cells ◽  
Cell Surface Molecule ◽  
Stem Cell Isolation

Human post-natal stem cells possess a great potential to be utilized in stem-cell-mediated clinical therapies and tissue engineering. It is not known whether cryopreserved human tissues contain functional post-natal stem cells. In this study, we utilized human periodontal ligament to test the hypothesis that cryopreserved human periodontal ligament contains retrievable post-natal stem cells. These cryopreserved periodontal ligament stem cells maintained normal periodontal ligament stem cell characteristics, including expression of the mesenchymal stem cell surface molecule STRO-1, single-colony-strain generation, multipotential differentiation, cementum/periodontal-ligament-like tissue regeneration, and a normal diploid karyotype. Collectively, this study provides valuable evidence demonstrating a practical approach to the preservation of solid-frozen human tissues for subsequent post-natal stem cell isolation and tissue regeneration. The present study demonstrates that human post-natal stem cells can be recovered from cryopreserved human periodontal ligament, thereby providing a practical clinical approach for the utilization of frozen tissues for stem cell isolation.

scholarly journals A Preliminary Technique for the Isolation and Culture of Brown Trout (Salmo trutta macrostigma, Dumeril, 1858) Spermatogonial Stem Cell

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Sehriban CEK-YALNIZ ◽  
Kamuran Umut YARAS
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Spermatogonial Stem Cells ◽  
Cell Isolation ◽  
Spermatogonial Stem Cell ◽  
Culture Techniques ◽  
Brown Trout Salmo Trutta ◽  
Stem Cell Isolation

This study was aimed to find a practical technique for isolation and culture spermatogonial stem cells from male brown trout (Salmo trutta macrostigma). Twelve wild juvenile male were obtained from Kılıç Trout Fish Farm (Kahramanmaraş, Turkey). The juveniles were taken alive to the aquaria unit and stored in a 1000-liter capacity fiberglass tank.  In order to identify the best size, age and testis structure of S.t. macrostigma for spermatogonial stem cell isolation and culture. Morphological and histological testis conditions were assessed. Fish were anesthetized with 0.04% 2-phenoxethanol. The surface of the fish was sterilized with 70% ethanol. Twelve fish were divided into two groups for enzyme digestion, and each group was divided into two replicates (three fish per replicate). Testis tissue of group one were digested by 0.25% trypsin- EDTA, and testis tissues of group two were digested by 0.05% trypsin-EDTA. At the end of the trial, first, the best age, size and weight of the male fish for spermatogonial stem cell isolation and culture were identified as 5+ month old, 12.13±1.5 cm, 19, 25±7.05 g respectively. Then, the highest spermatogonial stem cells were measured in the stage one and two of the testes. Finally, isolation and culture conditions were optimized for male S.t. macrostigma. Spermatogonial stem cell isolation and culture techniques were defined for fish in order to be used in surrogate reproduction technologies and gene transfer systems.

Measured Levels of Human Adipose Tissue–Derived Stem Cells in Adipose Tissue Is Strongly Dependent on Harvesting Method and Stem Cell Isolation Technique

2020 ◽  
Vol 145 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Toke Alstrup ◽  
Marco Eijken ◽  
Mette Eline Brunbjerg ◽  
Niels Hammer-Hansen ◽  
Bjarne K. Møller ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Human Adipose Tissue ◽  
Cell Isolation ◽  
Isolation Technique ◽  
Harvesting Method ◽  
Stem Cell Isolation

scholarly journals Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Sean M. Devitt ◽  
Cynthia M. Carter ◽  
Raia Dierov ◽  
Scott Weiss ◽  
Robert P. Gersch ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Cell Isolation ◽  
Stem Cell Markers ◽  
Adipose Derived Stem Cells ◽  
Patient Age ◽  
Patient Âgé ◽  
Stem Cell Isolation

We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2–1159 days) from patients of varying ages (26–62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation duration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were no significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient age. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently, longer cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with continued cell growth. Patient age does not significantly impact stem cell isolation, viability, or growth. Cryopreservation of adipose tissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages.

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