scholarly journals An Electrokinetically-Driven Microchip for Rapid Entrapment and Detection of Nanovesicles

Micromachines ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 11
Author(s):  
Leilei Shi ◽  
Leyla Esfandiari
Keyword(s):  
Electrical Impedance ◽  
Disease Diagnosis ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Early Disease ◽  
Non Invasive ◽  
Diagnosis And Prognosis ◽  
Rapid Characterization ◽  
Impedance Sensor

Electrical Impedance Spectroscopy (EIS) has been widely used as a label-free and rapid characterization method for the analysis of cells in clinical research. However, the related work on exosomes (40–150 nm) and the particles of similar size has not yet been reported. In this study, we developed a new Lab-on-a-Chip (LOC) device to rapidly entrap a cluster of sub-micron particles, including polystyrene beads, liposomes, and small extracellular vesicles (exosomes), utilizing an insulator-based dielectrophoresis (iDEP) scheme followed by measuring their impedance utilizing an integrated electrical impedance sensor. This technique provides a label-free, fast, and non-invasive tool for the detection of bionanoparticles based on their unique dielectric properties. In the future, this device could potentially be applied to the characterization of pathogenic exosomes and viruses of similar size, and thus, be evolved as a powerful tool for early disease diagnosis and prognosis.

Electrical impedance spectroscopy for the characterization of skin barrier in atopic dermatitis

Allergy ◽  
2021 ◽  
Author(s):  
Arturo O. Rinaldi ◽  
Angelica Korsfeldt ◽  
Siobhan Ward ◽  
Daniel Burla ◽  
Anita Dreher ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Impedance Spectroscopy ◽  
Electrical Impedance ◽  
Skin Barrier ◽  
Electrical Impedance Spectroscopy

Virus induced hepatocellular carcinoma (HCC) and protein biomarkers

2021 ◽  
Author(s):  
Hamza Abbas Jaffari ◽  
Sumaira Mazhar
Keyword(s):  
Hepatocellular Carcinoma ◽  
Early Stage ◽  
Imaging Techniques ◽  
Disease Diagnosis ◽  
Protein Biomarkers ◽  
Early Disease ◽  
Detection Systems ◽  
Non Invasive ◽  
Viral Hepatitis B ◽  
Current Article

Hepatocellular carcinoma (HCC) is a standout amongst the most widely recognized cancers around the world, and just as the alcoholic liver disease it is also progressed by extreme viral hepatitis B or C. At the early stage of the disease, numerous patients are asymptomatic consequently late diagnosis of HCC occurs resulting in expensive surgical resection or transplantation. On the basis of the alpha fetoprotein (AFP) estimation, combined with the ultrasound and other sensitive imaging techniques used, the non-invasive detection systems are available. For early disease diagnosis and its use in the effective treatment of HCC patients, the identification of HCC biomarkers has provided a breakthrough utilizing the molecular genetics and proteomics. In the current article, most recent reports on the protein biomarkers of HBV or HCV-related HCC and their co-evolutionary association with liver cancer are reviewed.

scholarly journals Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 832
Author(s):  
Lexi Crowell ◽  
Juan Yakisich ◽  
Brian Aufderheide ◽  
Tayloria Adams
Keyword(s):  
Impedance Spectroscopy ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Cellular Response ◽  
Electrical Impedance ◽  
Electrical Impedance Spectroscopy ◽  
Cell Behavior ◽  
Drug Resistant ◽  
Cancerous Cells

Electrical impedance spectroscopy (EIS) is an electrokinetic method that allows for the characterization of intrinsic dielectric properties of cells. EIS has emerged in the last decade as a promising method for the characterization of cancerous cells, providing information on inductance, capacitance, and impedance of cells. The individual cell behavior can be quantified using its characteristic phase angle, amplitude, and frequency measurements obtained by fitting the input frequency-dependent cellular response to a resistor–capacitor circuit model. These electrical properties will provide important information about unique biomarkers related to the behavior of these cancerous cells, especially monitoring their chemoresistivity and sensitivity to chemotherapeutics. There are currently few methods to assess drug resistant cancer cells, and therefore it is difficult to identify and eliminate drug-resistant cancer cells found in static and metastatic tumors. Establishing techniques for the real-time monitoring of changes in cancer cell phenotypes is, therefore, important for understanding cancer cell dynamics and their plastic properties. EIS can be used to monitor these changes. In this review, we will cover the theory behind EIS, other impedance techniques, and how EIS can be used to monitor cell behavior and phenotype changes within cancerous cells.

A Review of Electrical Impedance Characterization of Cells for Label-Free and Real-Time Assays

2019 ◽  
Vol 13 (4) ◽  
pp. 295-305 ◽  
Author(s):  
Hien T. Ngoc Le ◽  
Junsub Kim ◽  
Jinsoo Park ◽  
Sungbo Cho
Keyword(s):  
Real Time ◽  
Electrical Impedance ◽  
Label Free ◽  
Impedance Characterization

Measurements of Nonlinear Electrical Impedances by Virtue of Induced Conformational Changes in DNAs

2010 ◽  
Vol 22 (5) ◽  
pp. 601-607 ◽  
Author(s):  
Takatoki Yamamoto ◽  
◽  
Sangwook Lee ◽  
Teruo Fujii ◽  
Keyword(s):  
Aqueous Solution ◽  
Conformational Changes ◽  
Electrical Impedance ◽  
Dna Analysis ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Impedance Sensing ◽  
Fully Integrated ◽  
Impedance Response ◽  
Strength Dependence

A method for label-free electrical impedance sensing of DNA is proposed, and experimentally demonstrated using a micro Electrical Impedance Spectroscopy (µ- EIS) device. The method features not only the detection of DNA without any labelling, but also the control of the conformation that would enhance the electrical impedance signal. In order to conduct semiautomated measurements controlled by an external PC, a microfluidic chip made of a silicone elastomer of polydimethylsiloxane (PDMS), a measurement chip embedded with micro-electrodes, and a micropump chip are fully integrated in the µ-EIS device. The µ-EIS device is capable of detecting DNA concentrations of a few nM in aqueous solution of a few pL in volume by virtue of the conformation-enhanced nonlinear impedance response. As a first demonstration of conformational-change-induced DNA analysis, the frequency and the electric field strength dependence of various lengths of DNA are evaluated.

Microwave Assisted Blending-Intercalation of Ion-Conductor Polymers into Layered Silicates

1998 ◽  
Vol 519 ◽  
Author(s):  
P. Aranda ◽  
J.C. Galván ◽  
E. Ruiz-Hitzky
Keyword(s):  
Electrical Impedance ◽  
Polymer Melt ◽  
Electrical Impedance Spectroscopy ◽  
Layered Silicates ◽  
Alternative Procedure ◽  
Ion Conductor ◽  
Microwave Assisted ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

AbstractOrgano-inorganic hybrid nancomposites derived from poly(ethylene oxide) and montmorillonite silicate are prepared by an alternative procedure to classical polymer intercalation either from solutions or by polymer melt-intercalation. XRD, FTIR, DSC, elemental microanalysis and SEM techniques are applied for structural characterization of the resulting materials. The electrical properties of these nanocomposites are studied by means of the electrical impedance spectroscopy (EIS) showing enhanced ionic conductivity compared to similar samples prepared by intercalation from solution.

scholarly journals Bioimpedance and NIR for non-invasive assessment of blood glucose

2019 ◽  
Vol 10 (1) ◽  
pp. 133-138 ◽  
Author(s):  
Jan-Hugo Andersen ◽  
Olav Bjerke ◽  
Fatos Blakaj ◽  
Vilde Moe Flugsrud ◽  
Fredrik Alstad Jacobsen ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Electrical Impedance ◽  
Near Infrared ◽  
Soft Drink ◽  
Electrical Impedance Spectroscopy ◽  
Electrical Measurements ◽  
High Blood Glucose ◽  
Glucose Levels ◽  
Non Invasive ◽  
Blood Glucose Levels

Abstract Sixteen volunteers each drank 700 ml sugar-containing soft drink during two successive periods and the blood sugar was measured at 10 min intervals together with electrical impedance spectroscopy and near infrared spectroscopy (NIR). A maximum correlation of 0.46 was found for the electrical measurements but no clear separation between low and high blood glucose levels were found in the NIR measurements. The latter was attributed to the experimental design where the NIR probe was removed from the skin between each measurement.

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