scholarly journals Fabrication of 512-Channel Geometrical Passive Breakup Device for High-Throughput Microdroplet Production

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 709 ◽  
Author(s):  
Chul Min Kim ◽  
Gyu Man Kim
Keyword(s):  
Size Distribution ◽  
Hydraulic Resistance ◽  
Microfluidic Device ◽  
High Throughput ◽  
Mass Production ◽  
High Speed ◽  
Continuous Phase ◽  
Liquid Plug ◽  
Pass Through ◽  
Analytical Approaches

We present a 512-microchannel geometrical passive breakup device for the mass production of microdroplets. The mass production is achieved through the passive breakup of a droplet into two droplets. The microchannel geometry in the microfluidic device was designed and optimized by focusing on stable droplet splitting for microdroplet preparation and minimizing the hydraulic resistance of the microchannel for achieving high throughput; the minimization of hydraulic resistance was achieved by employing analytical approaches. A total of 512 microdroplets could be prepared from a single liquid plug by making the liquid plug pass through nine sequential T-junctions in the microfluidic device, which led to the splitting of droplets. The microfluidic device was fabricated using conventional photolithography and polydimethylsiloxane (PDMS) casting. We estimated the performance of the microfluidic device in terms of the size distribution and production rate of microdroplets. Microdroplets with a diameter of 40.0 ± 2.2 µm were prepared with a narrow size distribution (coefficient of variation (CV) < 5.5%) for flow rates of disperse (Qd) and continuous phase (Qc) of 2 and 3 mL/h, respectively. Microdroplet production rates were measured using a high-speed camera. Furthermore, monodisperse microdroplets were prepared at 42.7 kHz for Qd and Qc of 7 and 15 mL/h, respectively. Finally, the feasibility of the fabricated microfluidic device was verified by using it to prepare biodegradable chitosan microspheres.

Microfluidics of blood in blood vessels stenosis

2016 ◽  
Vol 11 (2) ◽  
pp. 210-217 ◽  
Author(s):  
A.T. Akhmetov ◽  
A.A. Valiev ◽  
A.A. Rakhimov ◽  
S.P. Sametov ◽  
R.R. Habibullina
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Hydraulic Resistance ◽  
Microfluidic Device ◽  
Human Body ◽  
Vascular System ◽  
Hydrodynamic Conditions ◽  
Microstructure Change ◽  
Healthy Part

It is mentioned in the paper that hydrodynamic conditions of a flow in blood vessels with the stenosis are abnormal in relation to the total hemodynamic conditions of blood flow in a vascular system of a human body. A microfluidic device developed with a stepped narrowing for studying of the blood flow at abnormal conditions allowed to reveal blood structure in microchannels simulating the stenosis. Microstructure change is observed during the flow of both native and diluted blood through the narrowing. The study of hemorheological properties allowed us to determine an increasing contribution of the hydraulic resistance of the healthy part of the vessel during the stenosis formation.

scholarly journals Modeling the Excess Velocity of Low-Viscous Taylor Droplets in Square Microchannels

Fluids ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 162 ◽  
Author(s):  
Thorben Helmers ◽  
Philip Kemper ◽  
Jorg Thöming ◽  
Ulrich Mießner
Keyword(s):  
High Speed ◽  
Process Intensification ◽  
Continuous Phase ◽  
Channel Cross Section ◽  
Optimization Approach ◽  
Mean Flow ◽  
Bypass Flow ◽  
Wall Film ◽  
Proposed Model ◽  
The Mean

Microscopic multiphase flows have gained broad interest due to their capability to transfer processes into new operational windows and achieving significant process intensification. However, the hydrodynamic behavior of Taylor droplets is not yet entirely understood. In this work, we introduce a model to determine the excess velocity of Taylor droplets in square microchannels. This velocity difference between the droplet and the total superficial velocity of the flow has a direct influence on the droplet residence time and is linked to the pressure drop. Since the droplet does not occupy the entire channel cross-section, it enables the continuous phase to bypass the droplet through the corners. A consideration of the continuity equation generally relates the excess velocity to the mean flow velocity. We base the quantification of the bypass flow on a correlation for the droplet cap deformation from its static shape. The cap deformation reveals the forces of the flowing liquids exerted onto the interface and allows estimating the local driving pressure gradient for the bypass flow. The characterizing parameters are identified as the bypass length, the wall film thickness, the viscosity ratio between both phases and the C a number. The proposed model is adapted with a stochastic, metaheuristic optimization approach based on genetic algorithms. In addition, our model was successfully verified with high-speed camera measurements and published empirical data.

scholarly journals Fully Automated Cultivation of Adipose-Derived Stem Cells in the StemCellDiscovery—A Robotic Laboratory for Small-Scale, High-Throughput Cell Production Including Deep Learning-Based Confluence Estimation

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 575
Author(s):  
Jelena Ochs ◽  
Ferdinand Biermann ◽  
Tobias Piotrowski ◽  
Frederik Erkens ◽  
Bastian Nießing ◽  
...  
Keyword(s):  
Stem Cells ◽  
Deep Learning ◽  
High Throughput ◽  
High Speed ◽  
New Technologies ◽  
Human Mesenchymal Stem Cells ◽  
Simulation Software ◽  
System Capacity ◽  
Small Scale ◽  
Production Environment

Laboratory automation is a key driver in biotechnology and an enabler for powerful new technologies and applications. In particular, in the field of personalized therapies, automation in research and production is a prerequisite for achieving cost efficiency and broad availability of tailored treatments. For this reason, we present the StemCellDiscovery, a fully automated robotic laboratory for the cultivation of human mesenchymal stem cells (hMSCs) in small scale and in parallel. While the system can handle different kinds of adherent cells, here, we focus on the cultivation of adipose-derived hMSCs. The StemCellDiscovery provides an in-line visual quality control for automated confluence estimation, which is realized by combining high-speed microscopy with deep learning-based image processing. We demonstrate the feasibility of the algorithm to detect hMSCs in culture at different densities and calculate confluences based on the resulting image. Furthermore, we show that the StemCellDiscovery is capable of expanding adipose-derived hMSCs in a fully automated manner using the confluence estimation algorithm. In order to estimate the system capacity under high-throughput conditions, we modeled the production environment in a simulation software. The simulations of the production process indicate that the robotic laboratory is capable of handling more than 95 cell culture plates per day.

High-speed and high-precision fluorescence-based cell count and viability assays using the Cellaca™ MX high-throughput cell counter

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S97
Author(s):  
J. Bell ◽  
Y. Huang ◽  
S. Yung ◽  
H. Qazi ◽  
C. Hernandez ◽  
...  
Keyword(s):  
Cell Count ◽  
High Precision ◽  
High Throughput ◽  
High Speed ◽  
Cell Counter

Gliding Flight of the White-Backed Vulture Gyps Africanus

1971 ◽  
Vol 55 (1) ◽  
pp. 13-38 ◽  
Author(s):  
C. J. PENNYCUICK
Keyword(s):  
High Speed ◽  
Lift Coefficient ◽  
The Body ◽  
East African ◽  
Induced Drag ◽  
Gliding Flight ◽  
Vertical Speed ◽  
Statistical Sense ◽  
Pass Through ◽  
Comparison Measurements

1. Glide-comparison measurements were made on ten species of East African soaring birds using a Schleicher ASK-14 powered sailplane. Horizontal and vertical speed differences between bird and glider were measured by a photographic method, and used to estimate the bird's horizontal and vertical speeds relative to the air. The analysis refers to the white-backed vulture, since by far the largest number of measurements was obtained on this species. 2. A regression analysis using a two-term approximation to the glide polar yielded an implausibly high estimate of induced drag, which was attributed to a lack of observations at lift coefficients above 0.72. An amended glide polar was constructed assuming elliptical lift distribution and a maximum lift coefficient of 1.6 to define the low-speed end, while the high-speed end was made to pass through the mean horizontal and sinking speeds of all the experimental points. This curve gave a minimum sinking speed of 0.76 m/s at a forward speed of 10 m/s, and a best glide ratio of 15.3:1 at 13 m/s. It did not differ significantly (in the statistical sense) from the original regression curve. 3. In comparing the estimated circling performance, based on the amended glide polar, with that of the ASK-14, it was concluded that the rates of sink of both should be comparable, but that the glider would require thermals with radii about 4.3 times as great as those needed to sustain the birds. The conclusions are consistent with experience of soaring in company with birds. 4. In an attempt to assess the adaptive significance of the low-aspect-ratio wings of birds specializing in thermal soaring, the white-backed vulture's circling performance was compared with that of an ‘albatross-shaped vulture’, an imaginary creature having the same mass as a white-backed vulture, combined with the body proportions of a wandering albatross. It appears that the real white-back would be at an advantage when trying to remain airborne in thermals with radii between 14 and 17 m, but that the albatross-shaped vulture would climb faster in all wider thermals; on account of its much better maximum glide ratio, it should also achieve higher cross-country speeds. It is concluded that the wing shape seen in vultures and storks is not an adaptation to thermal soaring as such, but is more probably a compromise dictated by take-off and landing requirements. 5. The doubts recently expressed by Tucker & Parrott (1970) about the results and conclusions of Raspet (1950a, b; 1960) are re-inforced by the present experience.

High Speed FPGA Based 128-bit Advance Encryption Standard (AES)

2021 ◽  
Vol 11 ◽  
Author(s):  
Ibrahem M. T. Hamidi ◽  
Farah S. H. Al-aassi
Keyword(s):  
High Throughput ◽  
Field Programmable Gate Array ◽  
High Speed ◽  
Design Tool ◽  
Advanced Encryption Standard ◽  
Clock Frequency ◽  
Advance Encryption Standard ◽  
Field Programmable ◽  
Internal Component ◽  
Gate Array

Aim: Achieve high throughput 128 bits FPGA based Advanced Encryption Standard. Background: Field Programmable Gate Array (FPGA) provides an efficient platform for design AES cryptography system. It provides the capability to control over each bit using HDL programming language such as VHDL and Verilog which results an output speed in Gbps rang. Objective: Use Field Programmable Gate Array (FPGA) to design high throughput 128 bits FPGA based Advanced Encryption Standard. Method: Pipelining technique has used to achieve maximum possible speed. The level of pipelining includes round pipelining and internal component pipelining where number of registers inserted in particular places to increase the output speed. The proposed design uses combinatorial logic to implement the byte substitution. The s-box implemented using composed field arithmetic with 7 stages of pipelining to reduce the combinatorial logic level. The presented model has implemented using VHDL in Xilinix ISETM 14.4 design tool. Result: The achieved results were 18.55 Gbps at a clock frequency of 144.96 MHz and area of 1568 Slices in Spartan3 xc3s1000 hardware. Conclusion: The results show that the proposed design reaches a high throughput with acceptable area usage compare with other designs in the literature.

Effect of Shear and Water Cut on Phase Inversion and Droplet Size Distribution in Oil–Water Flow

2018 ◽  
Vol 141 (3) ◽  
Author(s):  
Mo Zhang ◽  
Ramin Dabirian ◽  
Ram S. Mohan ◽  
Ovadia Shoham
Keyword(s):  
Size Distribution ◽  
Droplet Size ◽  
Phase Inversion ◽  
Droplet Size Distribution ◽  
Continuous Phase ◽  
Dispersed Phase ◽  
Water Emulsion ◽  
Inversion Region ◽  
Oil Water ◽  
Water Cut

Oil–water dispersed flow occurs commonly in the petroleum industry during the production and transportation of crudes. Phase inversion occurs when the dispersed phase grows into the continuous phase and the continuous phase becomes the dispersed phase caused by changes in the composition, interfacial properties, and other factors. Production equipment, such as pumps and chokes, generates shear in oil–water mixture flow, which has a strong effect on phase inversion phenomena. The objective of this paper is to investigate the effects of shear intensity and water cut (WC) on the phase inversion region and also the droplet size distribution. A state-of-the-art closed-loop two phase (oil–water) flow facility including a multipass gear pump and a differential dielectric sensor (DDS) is used to identify the phase inversion region. Also, the facility utilizes an in-line droplet size analyzer (a high speed camera), to record real-time videos of oil–water emulsion to determine the droplet size distribution. The experimental data for phase inversion confirm that as shear intensity increases, the phase inversion occurs at relatively higher dispersed phase fractions. Also the data show that oil-in-water emulsion requires larger dispersed phase volumetric fraction for phase inversion as compared with that of water-in-oil emulsion under the same shear intensity conditions. Experiments for droplet size distribution confirm that larger droplets are obtained for the water continuous phase, and increasing the dispersed phase volume fraction leads to the creation of larger droplets.

The Aerodynamic Characteristics of the Pantograph When the Train Passes Through the Tunnel

2017 ◽  
Author(s):  
Dilong Guo ◽  
Wen Liu ◽  
Junhao Song ◽  
Ye Zhang ◽  
Guowei Yang
Keyword(s):  
High Speed ◽  
Aerodynamic Force ◽  
Maximum Amplitude ◽  
Aerodynamic Characteristics ◽  
High Speed Train ◽  
Expansion Waves ◽  
Compression And Expansion ◽  
High Speed Trains ◽  
Current Characteristics ◽  
Pass Through

The aerodynamic force acting on the pantograph by the airflow is obviously unsteady and has a certain vibration frequency and amplitude, while the high-speed train passes through the tunnel. In addition to the unsteady behavior in the open-air operation, the compressive and expansion waves in the tunnel will be generated due to the influence of the blocking ratio. The propagation of the compression and expansion waves in the tunnel will affect the pantograph pressure distribution and cause the pantograph stress state to change significantly, which affects the current characteristics of the pantograph. In this paper, the aerodynamic force of the pantograph is studied with the method of the IDDES combined with overset grid technique when high speed train passes through the tunnel. The results show that the aerodynamic force of the pantograph is subjected to violent oscillations when the pantograph passes through the tunnel, especially at the entrance of the tunnel, the exit of the tunnel and the expansion wave passing through the pantograph. The changes of the pantograph aerodynamic force can reach a maximum amplitude of 106%. When high-speed trains pass through tunnels at different speeds, the aerodynamic coefficients of the pantographs are roughly the same.

scholarly journals EVQuant; high-throughput quantification and characterization of extracellular vesicle (sub)populations

2020 ◽  
Author(s):  
T.A. Hartjes ◽  
J.A. Slotman ◽  
M.S. Vredenbregt ◽  
N. Dits ◽  
R. Van der Meel ◽  
...  
Keyword(s):  
Protein Content ◽  
Size Distribution ◽  
High Throughput ◽  
Liquid Biopsy ◽  
Ideal Liquid ◽  
Extracellular Vesicle ◽  
Cell Of Origin ◽  
Clinical Implementation ◽  
High Throughput Assay

AbstractExtracellular vesicles (EVs) reflect the cell of origin in terms of nucleic acids and protein content. They are found in biofluids and represent an ideal liquid biopsy biomarker source for many diseases. Unfortunately, clinical implementation is limited by available technologies for EV analysis. We have developed a simple, robust and sensitive microscopy-based high-throughput assay (EVQuant) to overcome these limitations and allow widespread use in the EV community. The EVQuant assay can detect individual immobilized EVs as small as 35 nm and determine their concentration in biofluids without extensive EV isolation or purification procedures. It can also identify specific EV subpopulations based on combinations of biomarkers and is used here to identify prostate-derived urinary EVs as CD9-/CD63+. Moreover, characterization of individual EVs allows analysis of their size distribution. The ability to identify, quantify and characterize EV (sub-)populations in high-throughput substantially extents the applicability of the EVQuant assay over most current EV quantification assays.

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