scholarly journals Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

Micromachines ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 399 ◽  
Author(s):  
Shinako Bansai ◽  
Takashi Morikura ◽  
Hiroaki Onoe ◽  
Shogo Miyata
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Skeletal Muscles ◽  
Malignant Tumors ◽  
Three Dimensional ◽  
Living Cells ◽  
Cyclic Stretch ◽  
Core Shell ◽  
Custom Made ◽  
A Cell

Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation.

scholarly journals Investigation of microvascular morphological measures for skeletal muscle tissue oxygenation by image-based modelling in three dimensions

2017 ◽  
Vol 14 (135) ◽  
pp. 20170635 ◽  
Author(s):  
B. Zeller-Plumhoff ◽  
K. R. Daly ◽  
G. F. Clough ◽  
P. Schneider ◽  
T. Roose
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Cross Sections ◽  
Tissue Oxygenation ◽  
Volume Fraction ◽  
Three Dimensional ◽  
Capillary Density ◽  
Three Dimensions ◽  
Two Dimensional ◽  
Contrast Imaging

The supply of oxygen in sufficient quantity is vital for the correct functioning of all organs in the human body, especially for skeletal muscle during exercise. Traditionally, microvascular oxygen supply capability is assessed by the analysis of morphological measures on transverse cross-sections of muscle, e.g. capillary density or capillary-to-fibre ratio. In this work, we investigate the relationship between microvascular structure and muscle tissue oxygenation in mice. Phase contrast imaging was performed using synchrotron radiation computed tomography (SR CT) to visualize red blood cells (RBCs) within the microvasculature in mouse soleus muscle. Image-based mathematical modelling of the oxygen diffusion from the RBCs into the muscle tissue was subsequently performed, as well as a morphometric analysis of the microvasculature. The mean tissue oxygenation was then compared with the morphological measures of the microvasculature. RBC volume fraction and spacing (mean distance of any point in tissue to the closest RBC) emerged as the best predictors for muscle tissue oxygenation, followed by length density (summed RBC length over muscle volume). The two-dimensional measures of capillary density and capillary-to-fibre ratio ranked last. We, therefore, conclude that, in order to assess the states of health of muscle tissue, it is advisable to rely on three-dimensional morphological measures rather than on the traditional two-dimensional measures.

scholarly journals Printed Life

2012 ◽  
Vol 134 (01) ◽  
pp. 44-47 ◽  
Author(s):  
Jean Thilmany
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Body Part ◽  
Living Cells ◽  
Layer By Layer ◽  
Collagen Scaffolds ◽  
Three Dimensional Printing ◽  
A Cell ◽  
Dimensional Object ◽  
Dimensional Printing

This article discusses the advancement in bioprinting technology that would enable three-dimensional printing of living organs for transplant. Today, artificial or replacement tissue is commonly grown on collagen scaffolds that contain biological starter cells. The goal here is the growing of a biocompatible piece of tissue to repair or replace a patient’s own damaged body part, such as bone, cartilage, blood vessels, or skin. In future, bioprinting technology will allow making living organs for transplant. The method is much the same as additive manufacturing, in which a printer deposits a material, layer by layer, until a three-dimensional object is made. For bioprinting, the material used is likely to be living cells taken directly from the patient’s body and infused into an ink or gel to keep them alive. After printing, the material is incubated in a cell culture that mimics human body conditions until it fuses or becomes otherwise usable for implant.

scholarly journals Muscling in on the third dimension

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Mohsen Afshar Bakooshli ◽  
Penney M Gilbert
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Human Skeletal Muscle ◽  
Three Dimensional ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Muscle Disorders ◽  
The Third ◽  
Third Dimension ◽  
Skeletal Muscle Disorders

The development of a functional three-dimensional model of human skeletal muscle tissue could accelerate progress towards new and personalized treatments for skeletal muscle disorders.

scholarly journals Expression of sialyltransferases from the ST3Gal, ST6Gal and ST6GalNAc families in mouse skeletal muscle and mouse C2C12 myotube cell cultures

2021 ◽  
Author(s):  
Rositsa Milcheva ◽  
Any K. Georgieva ◽  
Katerina S. Todorova ◽  
Svetlozara L. Petkova
Keyword(s):  
Skeletal Muscle ◽  
Cell Cultures ◽  
Muscle Tissue ◽  
Skeletal Muscles ◽  
C2c12 Cell ◽  
Lectin Histochemistry ◽  
Skeletal Muscle Tissue ◽  
C2c12 Myotubes ◽  
Sialylated Glycoproteins ◽  
C2c12 Myotube

Abstract I. Background. In skeletal muscles the sialic acids have a great significance for their functional maintenance and proper structural organization. Our work for the first time described the expressions of ST3Gal, ST6Gal and ST6GalNAc sialyltransferases specific for glycoproteins in mouse skeletal muscles and murine C2C12 myotube cell cultures.II. Methods and Results. Lectin histochemistry, cytochemistry and lectin blot were used to demonstrate the membrane localization and the electrophoretic profiles of α-2,3- and α-2,6-sialylated glycoproteins. The expression levels of sialyltransferases were analyzed by real time RT-PCR and western blot. The enzymes ST6Gal2 and ST6GalNAc1 were not expressed in skeletal muscle tissue and C2C12 myotubes. In both experimental groups mRNAs of the ST3Gal family prevailed over the mRNA expressions of the ST6Gal and ST6GalNAc families. The profiles of STR expressions showed differences between the two experimental groups, illustrated by the absence of expressions of the mRNA for the ST3Gal6 and ST3GalNAc3 enzymes in the C2C12 cell samples and by the different shares of the enzymes ST3Gal3 and ST3Gal4 in both experimental groups. The different patterns of enzyme expressions in both experimental groups corresponded with differences between their α-2,3- and α-2,6-sialylated glycoprotein profiles.III. Conclusions. These results could be a useful addendum to the knowledge concerning the glycosylation of the skeletal muscle tissue. In addition, this report would be helpful and informative for any researches in future where the C2C12 myotube cell cultures will take a place as an experimental model.

Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat

Nature Food ◽  
2020 ◽  
Vol 1 (4) ◽  
pp. 210-220 ◽  
Author(s):  
Tom Ben-Arye ◽  
Yulia Shandalov ◽  
Shahar Ben-Shaul ◽  
Shira Landau ◽  
Yedidya Zagury ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Soy Protein ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
Protein Scaffolds ◽  
Bovine Skeletal Muscle

scholarly journals Evaluation of Skeletal Muscle Function and Effects of Early Rehabilitation during Acute Heart Failure: Rationale and Study Design

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Kinga Węgrzynowska-Teodorczyk ◽  
Agnieszka Siennicka ◽  
Krystian Josiak ◽  
Robert Zymliński ◽  
Monika Kasztura ◽  
...  
Keyword(s):  
Heart Failure ◽  
Skeletal Muscle ◽  
Acute Heart Failure ◽  
Muscle Tissue ◽  
Muscle Function ◽  
Skeletal Muscles ◽  
Group Versus ◽  
The Body ◽  
Skeletal Muscle Function ◽  
Peripheral Tissues

Background. Acute heart failure (AHF) is associated with disturbances of the peripheral perfusion leading to the dysfunction of many organs. Consequently, an episode of AHF constitutes a “multiple organ failure” which may also affect the skeletal muscles. However, the abnormalities within skeletal muscles during AHF have not been investigated so far. The aim of this project is to comprehensively evaluate skeletal muscles (at a functional and tissue level) during AHF. Methods. The study will include ≥63 consecutive AHF patients who will be randomized into 2 groups: ≥42 with cardiac rehabilitation group versus ≥21 with standard pharmacotherapy alone. The following tests will be conducted on the first and last day of hospitalization, at rest and after exercise, and 30 days following the discharge: clinical evaluation, medical interview, routine physical examination, echocardiography, and laboratory tests (including the assessment of NT-proBNP, inflammatory markers, and parameters reflecting the status of the kidneys and the liver); hemodynamic evaluation, noninvasive determination of cardiac output and systemic vascular resistance using the impedance cardiography; evaluation of biomarkers reflecting myocyte damage, immunochemical measurements of tissue-specific enzymatic isoforms; evaluation of skeletal muscle function, using surface electromyography (sEMG) (maximum tonus of the muscles will be determined along with the level of muscular fatigability); evaluation of muscle tissue perfusion, assessed on the basis of the oxygenation level, with noninvasive direct continuous recording of perfusion in peripheral tissues by local tissue oximetry, measured by near-infrared spectroscopy (NIRS). Results and Conclusions. Our findings will demonstrate that the muscle tissue is another area of the body which should be taken into consideration in the course of treatment of AHF, requiring a development of targeted therapeutic strategies, such as a properly conducted rehabilitation.

scholarly journals Smart ECM-Based Electrospun Biomaterials for Skeletal Muscle Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1781 ◽  
Author(s):  
Sara Politi ◽  
Felicia Carotenuto ◽  
Antonio Rinaldi ◽  
Paolo Di Nardo ◽  
Vittorio Manzari ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Muscle Tissue ◽  
Tissue Regeneration ◽  
Cell Function ◽  
Three Dimensional ◽  
Supporting Cell ◽  
Electrospinning Process ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Regeneration

The development of smart and intelligent regenerative biomaterials for skeletal muscle tissue engineering is an ongoing challenge, owing to the requirement of achieving biomimetic systems able to communicate biological signals and thus promote optimal tissue regeneration. Electrospinning is a well-known technique to produce fibers that mimic the three dimensional microstructural arrangements, down to nanoscale and the properties of the extracellular matrix fibers. Natural and synthetic polymers are used in the electrospinning process; moreover, a blend of them provides composite materials that have demonstrated the potential advantage of supporting cell function and adhesion. Recently, the decellularized extracellular matrix (dECM), which is the noncellular component of tissue that retains relevant biological cues for cells, has been evaluated as a starting biomaterial to realize composite electrospun constructs. The properties of the electrospun systems can be further improved with innovative procedures of functionalization with biomolecules. Among the various approaches, great attention is devoted to the “click” concept in constructing a bioactive system, due to the modularity, orthogonality, and simplicity features of the “click” reactions. In this paper, we first provide an overview of current approaches that can be used to obtain biofunctional composite electrospun biomaterials. Finally, we propose a design of composite electrospun biomaterials suitable for skeletal muscle tissue regeneration.

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