scholarly journals Development and Validation of a Smartphone-Based Near-Infrared Optical Imaging Device to Measure Physiological Changes In-Vivo

Micromachines ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 180 ◽  
Author(s):  
Kacie Kaile ◽  
Anuradha Godavarty
Keyword(s):  
Near Infrared ◽  
Optical Power ◽  
Optical Filters ◽  
Noise Removal ◽  
Light Sources ◽  
Decomposition Algorithms ◽  
Physiological Changes ◽  
Imaging Device ◽  
Nir Imaging

Smartphone-based technologies for medical imaging purposes are limited, especially when it involves the measurement of physiological information of the tissues. Herein, a smartphone-based near-infrared (NIR) imaging device was developed to measure physiological changes in tissues across a wide area and without contact. A custom attachment containing multiple multi-wavelength LED light sources (690, 800, and 840 nm; and <4 mW of optical power per LED), source driver, and optical filters and lenses was clipped onto a smartphone that served as the detector during data acquisition. The ability of the device to measure physiological changes was validated via occlusion studies on control subjects. Noise removal techniques using singular value decomposition algorithms effectively removed surface noise and distinctly differentiated the physiological changes in response to occlusion. In the long term, the developed smartphone-based NIR imaging device with capabilities to capture physiological changes will be a great low-cost alternative for clinicians and eventually for patients with chronic ulcers and bed sores, and/or in pre-screening for potential ulcers in diabetic subjects.

scholarly journals Imaging of β-amyloid plaques by near infrared fluorescent tracers: a new frontier for chemical neuroscience

2015 ◽  
Vol 44 (7) ◽  
pp. 1807-1819 ◽  
Author(s):  
Matteo Staderini ◽  
María Antonia Martín ◽  
Maria Laura Bolognesi ◽  
J. Carlos Menéndez
Keyword(s):  
Near Infrared ◽  
Amyloid Plaques ◽  
Fluorescent Tracers ◽  
Non Invasive ◽  
Nir Imaging ◽  
New Frontier ◽  
Invasive Method ◽  
Β Amyloid

Near infrared (NIR) imaging is a promising and non-invasive method to visualize amyloid plaquesin vivo.

In-vivo near-infrared optical imaging of growing osteosarcoma cell lesions xenografted in mice: dual-channel quantitative evaluation of volume and mineralization

2011 ◽  
Vol 52 (9) ◽  
pp. 978-988 ◽  
Author(s):  
Hitoshi Nakayama ◽  
Tomoyuki Kawase ◽  
Kazuhiro Okuda ◽  
Larry F Wolff ◽  
Hiromasa Yoshie
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Imaging Technique ◽  
Ex Vivo ◽  
Acquisition Time ◽  
Osteosarcoma Cell ◽  
Dual Channel ◽  
Nir Imaging ◽  
Ex Vivo Imaging

Background In a previous study using a rodent osteosarcoma-grafted rat model, in which cell-dependent mineralization was previously demonstrated to proportionally increase with growth, we performed a quantitative analysis of mineral deposit formation using 99mTc-HMDP and found some weaknesses, such as longer acquisition time and narrower dynamic ranges (i.e. images easily saturated). The recently developed near-infrared (NIR) optical imaging technique is expected to non-invasively evaluate changes in living small animals in a quantitative manner. Purpose To test the feasibility of NIR imaging with a dual-channel system as a better alternative for bone scintigraphy by quantitatively evaluating mineralization along with the growth of osteosarcoma lesions in a mouse-xenograft model. Material and Methods The gross volume and mineralization of osteosarcoma lesions were evaluated in living mice simultaneously with dual-channels by NIR dye-labeled probes, 2-deoxyglucose (DG) and pamidronate (OS), respectively. To verify these quantitative data, retrieved osteosarcoma lesions were then subjected to ex-vivo imaging, weighing under wet conditions, microfocus-computed tomography (μCT) analysis, and histopathological examination. Results Because of less scattering and no anatomical overlapping, as generally shown, specific fluorescence signals targeted to the osteosarcoma lesions could be determined clearly by ex-vivo imaging. These data were well positively correlated with the in-vivo imaging data ( r > 0.8, P < 0.02). Other good to excellent correlations ( r > 0.8, P < 0.02) were observed between DG accumulation and tumor gross volume and between OS accumulation and mineralization volume. Conclusion This in-vivo NIR imaging technique using DG and OS is sensitive to the level to simultaneously detect and quantitatively evaluate the growth and mineralization occuring in this type of osteosarcoma lesions of living mice without either invasion or sacrifice. By possible mutual complementation, this dual imaging system might be useful for accurate diagnosis even in the presence of overlapping tissues.

scholarly journals Extending the Near Infrared Emission Range of Indium Phosphide Quantum Dots for Multiplexed In Vivo Imaging

2021 ◽  
Author(s):  
Alexander M. Saeboe ◽  
Alexey Y. Nikiforov ◽  
Reyhaneh Toufanian ◽  
Joshua C. Kays ◽  
Margaret Chern ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Indium Phosphide ◽  
Near Infrared ◽  
Infrared Emission ◽  
Full Potential ◽  
Lymph Node Imaging ◽  
Nir Imaging ◽  
Near Infrared Emission ◽  
Tunable Emission

AbstractThis report of the reddest emitting indium phosphide quantum dots (InP QDs) to date demonstrates tunable, near infrared (NIR) photoluminescence and fluorescence multiplexing in the first optical tissue window with a material that avoids toxic constituents. This synthesis overcomes the InP synthesis “growth bottleneck” and extends the emission peak of InP QDs deeper into the first optical tissue window using an inverted QD heterostructure. The ZnSe/InP/ZnS core/shell/shell structure is designed to produce emission from excitons with heavy holes confined in InP shells wrapped around larger-bandgap ZnSe cores and protected by a second shell of ZnS. The InP QDs exhibit InP shell thickness-dependent tunable emission with peaks ranging from 515 – 845 nm. The high absorptivity of InP leads to effective absorbance and photoexcitation of the QDs with UV, visible, and NIR wavelengths in particles with diameters of eight nanometers or less. These nanoparticles extend the range of tunable direct-bandgap emission from InP-based nanostructures, effectively overcoming a synthetic barrier that has prevented InP-based QDs from reaching their full potential as NIR imaging agents. Multiplexed lymph node imaging in a mouse model shows the potential of the NIR-emitting InP particles for in vivo imaging.

scholarly journals Microscale optoelectronic infrared-to-visible upconversion devices and their use as injectable light sources

2018 ◽  
Vol 115 (26) ◽  
pp. 6632-6637 ◽  
Author(s):  
He Ding ◽  
Lihui Lu ◽  
Zhao Shi ◽  
Dan Wang ◽  
Lizhu Li ◽  
...  
Keyword(s):  
Near Infrared ◽  
Biological Fluids ◽  
Visible Spectral Range ◽  
Infrared Light ◽  
Light Sources ◽  
Fully Integrated ◽  
Broadband Absorption ◽  
In Vivo Experiments

Optical upconversion that converts infrared light into visible light is of significant interest for broad applications in biomedicine, imaging, and displays. Conventional upconversion materials rely on nonlinear light-matter interactions, exhibit incidence-dependent efficiencies, and require high-power excitation. We report an infrared-to-visible upconversion strategy based on fully integrated microscale optoelectronic devices. These thin-film, ultraminiaturized devices realize near-infrared (∼810 nm) to visible [630 nm (red) or 590 nm (yellow)] upconversion that is linearly dependent on incoherent, low-power excitation, with a quantum yield of ∼1.5%. Additional features of this upconversion design include broadband absorption, wide-emission spectral tunability, and fast dynamics. Encapsulated, freestanding devices are transferred onto heterogeneous substrates and show desirable biocompatibilities within biological fluids and tissues. These microscale devices are implanted in behaving animals, with in vitro and in vivo experiments demonstrating their utility for optogenetic neuromodulation. This approach provides a versatile route to achieve upconversion throughout the entire visible spectral range at lower power and higher efficiency than has previously been possible.

scholarly journals Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2649
Author(s):  
Toshihiro Takamatsu ◽  
Yuichi Kitagawa ◽  
Kohei Akimoto ◽  
Ren Iwanami ◽  
Yuto Endo ◽  
...  
Keyword(s):  
Near Infrared ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Data Sets ◽  
Imaging Device ◽  
Tumor Implantation ◽  
Before And After ◽  
Validation Procedure ◽  
Leave One Out

In this study, a laparoscopic imaging device and a light source able to select wavelengths by bandpass filters were developed to perform multispectral imaging (MSI) using over 1000 nm near-infrared (OTN-NIR) on regions under a laparoscope. Subsequently, MSI (wavelengths: 1000–1400 nm) was performed using the built device on nine live mice before and after tumor implantation. The normal and tumor pixels captured within the mice were used as teaching data sets, and the tumor-implanted mice data were classified using a neural network applied following a leave-one-out cross-validation procedure. The system provided a specificity of 89.5%, a sensitivity of 53.5%, and an accuracy of 87.8% for subcutaneous tumor discrimination. Aggregated true-positive (TP) pixels were confirmed in all tumor-implanted mice, which indicated that the laparoscopic OTN-NIR MSI could potentially be applied in vivo for classifying target lesions such as cancer in deep tissues.

scholarly journals An effective method for detecting dorsal hand veins utilising near-infrared imaging technology

2021 ◽  
Vol 63 (2) ◽  
pp. 32-38
Author(s):  
Hai Thanh Le ◽  
◽  
Hien Thi Thu Pham ◽  
Keyword(s):  
Near Infrared ◽  
Infrared Imaging ◽  
Blood Collection ◽  
Imaging Device ◽  
Non Invasive ◽  
Nir Imaging ◽  
Dorsal Hand ◽  
Dorsal Hand Vein ◽  
High Level ◽  
Level Process

Intravenous access for blood collection and other related therapies is one of the most frequently practiced procedures in the modern medical system. The procedure requires complex training and experience, as it might cause dangerous nerve damage and subcutaneous bleeding. This paper proposes a dorsal hand vein detection method utilising the near-infrared (NIR) imaging device to segment and visualise the subcutaneous vein patterns on the skin directly. Applying NIR light has received substantial attention because of its non-invasive and revealing substantially more information than the visible one. The proposed method is divided into the low- and high-level processes. The captured image is smoothed and enhanced to make the vein patterns clearer in the low-level process. The pre-processed image is then segmented step by step to extract the vein features and eliminate the pseudo-vein regions precisely. Lastly, the detected veins are thinned to reduce the thickness and projected back onto the acquired image in the high-level process. The proposed method performs effectively in detecting the clear dorsal hand veins through the experiment with a processing time of 0.61s for the high-resolution image.

scholarly journals Liposomal Chitosan-N-Acetyl Cysteine Nanoparticle for In-Vitro/In-Vivo Near Infrared Fluorescent (NIR) Imaging

2019 ◽  
Author(s):  
Piyush Kumar ◽  
Timothy Van Treuren ◽  
Amalendu Ranjan ◽  
Jamboor K Vishwanatha
Keyword(s):  
Near Infrared ◽  
Infrared Imaging ◽  
Tumor Imaging ◽  
Ex Vivo ◽  
Breast Cancer Cell Lines ◽  
Nir Imaging ◽  
Negative Surface ◽  
Acetyl Cysteine

<p>In this work, we studied the effect of chitosan conjugated N-acetyl cysteine (CHT-NAC) coating on liposomal (DMPC:14 lysoPG: DSPE-2000-NH2) nanoparticles as a vehicle to cross the blood-brain barrier. The size of lipo-NP and Lipo-CHT-NAC NP were sub 50 nm with negative surface charge consistent with its use in an intravenous application. In vitro near infrared (NIR) imaging showed good cellular uptake in two triple-negative breast cancer cell lines (MDA-MB-231 and brain metastatic MDA-MB-831). Live (4-120 h) and ex-vivo near-infrared imaging at 24 h in nude mice showed the extended circulation of CHT-NAC Lipo-NP. These results demonstrated that Lipo-CHT-NAC NP could be used for metastatic brain tumor imaging.</p>

scholarly journals Liposomal Chitosan-N-Acetyl Cysteine Nanoparticle for In-Vitro/In-Vivo Near Infrared Fluorescent (NIR) Imaging

2019 ◽  
Author(s):  
Piyush Kumar ◽  
Timothy Van Treuren ◽  
Amalendu Ranjan ◽  
Jamboor K Vishwanatha
Keyword(s):  
Near Infrared ◽  
Infrared Imaging ◽  
Tumor Imaging ◽  
Ex Vivo ◽  
Breast Cancer Cell Lines ◽  
Nir Imaging ◽  
Negative Surface ◽  
Acetyl Cysteine

<p>In this work, we studied the effect of chitosan conjugated N-acetyl cysteine (CHT-NAC) coating on liposomal (DMPC:14 lysoPG: DSPE-2000-NH2) nanoparticles as a vehicle to cross the blood-brain barrier. The size of lipo-NP and Lipo-CHT-NAC NP were sub 50 nm with negative surface charge consistent with its use in an intravenous application. In vitro near infrared (NIR) imaging showed good cellular uptake in two triple-negative breast cancer cell lines (MDA-MB-231 and brain metastatic MDA-MB-831). Live (4-120 h) and ex-vivo near-infrared imaging at 24 h in nude mice showed the extended circulation of CHT-NAC Lipo-NP. These results demonstrated that Lipo-CHT-NAC NP could be used for metastatic brain tumor imaging.</p>

scholarly journals Optical Coherence Tomography for Three-Dimensional Imaging in the Biomedical Field: A Review

2021 ◽  
Vol 9 ◽  
Author(s):  
Shu Zheng ◽  
Yanru Bai ◽  
Zihao Xu ◽  
Pengfei Liu ◽  
Guangjian Ni
Keyword(s):  
Optical Coherence Tomography ◽  
High Speed ◽  
Near Infrared ◽  
Polarization State ◽  
Three Dimensional ◽  
Biological Research ◽  
Light Sources ◽  
Optical Coherence ◽  
Biomedical Field

Optical coherence tomography (OCT) has become a novel approach to noninvasive imaging in the past three decades, bringing a significant potential to biological research and medical biopsy in situ, particularly in three-dimensional (3D) in vivo conditions. Specifically, OCT systems using broad bandwidth sources, mainly centered at near-infrared-II, allow significantly higher imaging depth, as well as maintain a high-resolution and better signal-to-noise ratio than the traditional microscope, which avoids the scattering blur and thus obtains more details from delicate biological structures not just limited to the surface. Furthermore, OCT systems combined the spectrometer with novel light sources, such as multiplexed superluminescent diodes or ultra-broadband supercontinuum laser sources, to obtain sub-micron resolution imaging with high-speed achieve widespread clinical applications. Besides improving OCT performance, the functional extensions of OCT with other designs and instrumentations, taking polarization state or birefringence into account, have further improved OCT properties and functions. We summarized the conventional principle of OCT systems, including time-domain OCT, Fourier-domain OCT, and several typical OCT extensions, compared their different components and properties, and analyzed factors that affect OCT performance. We also reviewed current applications of OCT in the biomedical field, especially in hearing science, discussed existing limitations and challenges, and looked forward to future development, which may provide a guideline for those with 3D in vivo imaging desires.

Early optical detection of cerebral edema in vivo

2011 ◽  
Vol 114 (2) ◽  
pp. 470-477 ◽  
Author(s):  
Amandip S. Gill ◽  
Kiran F. Rajneesh ◽  
Christopher M. Owen ◽  
James Yeh ◽  
Mike Hsu ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Laser Diode ◽  
Cerebral Edema ◽  
Near Infrared ◽  
Optical Power ◽  
Optical Source ◽  
Optical Reflectance ◽  
Power Meter ◽  
Fiberoptic Probe

Object Cerebral edema is a significant cause of morbidity and mortality in diverse disease states. Currently, the means to detect progressive cerebral edema in vivo includes the use of intracranial pressure (ICP) monitors and/or serial radiological studies. However, ICP measurements exhibit a high degree of variability, and ICP monitors detect edema only after it becomes sufficient to significantly raise ICP. The authors report the development of 2 distinct minimally invasive fiberoptic near-infrared (NIR) techniques able to directly detect early cerebral edema. Methods Cytotoxic brain edema was induced in adult CD1 mice via water intoxication by intraperitoneal water administration (30% body weight intraperitoneally). An implantable dual-fiberoptic probe was stereotactically placed into the cerebral cortex and connected to optical source and detector hardware. Optical sources consisted of either broadband halogen illumination or a single-wavelength NIR laser diode, and the detector was a sensitive NIR spectrometer or optical power meter. In one subset of animals, a left-sided craniectomy was performed to obtain cortical biopsies for water-content determination to verify cerebral edema. In another subset of animals, an ICP transducer was placed on the contralateral cortex, which was synchronized to a computer and time stamped. Results Using either broadband illumination with NIR spectroscopy or single-wavelength laser diode illumination with optical power meter detection, the authors detected a reduction in NIR optical reflectance during early cerebral edema. The time intervals between water injection (Time Point 0), optical trigger (defined as a 2-SD change in optical reflectance from baseline), and defined threshold ICP values of 10, 15 and 20 mm Hg were calculated. Reduction in NIR reflectance occurred significantly earlier than any of the ICP thresholds (p < 0.001). Saline-injected control mice exhibited a steady baseline optical signal. There was a significant correlation between reflectance change and tissue specific gravity of the cortical biopsies, further validating the dual-fiberoptic probe as a direct measure of cerebral edema. Conclusions Compared with traditional ICP monitoring, the aforementioned minimally invasive NIR techniques allow for the significantly earlier detection of cerebral edema, which may be of clinical utility in the identification and thus early treatment of cerebral edema.

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