scholarly journals Empirical Modeling of Zn/ZnO Nanoparticles Decorated/Conjugated with Fotolon (Chlorine e6) Based Photodynamic Therapy towards Liver Cancer Treatment

Micromachines ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 60 ◽  
Author(s):  
Seemab Iqbal ◽  
Muhammad Fakhar-e-Alam ◽  
M. Atif ◽  
Nasar Ahmed ◽  
Aqrab -ul-Ahmad ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Zno Nanoparticles ◽  
Normal Liver ◽  
Empirical Modeling ◽  
In Vivo Model ◽  
P Value ◽  
X Ray ◽  
Chlorine E6

The current study is based on Zn/ZnO nanoparticles photodynamic therapy (PDT) mediated effects on healthy liver cells and cancerous cells. The synthesis of Zn/ZnO nanoparticles was accomplished using chemical and hydrothermal methods. The characterization of the synthesized nanoparticles was carried out using manifold techniques (e.g., transmission electron microscopy (TEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscopy (EDS)). In order to study the biotoxicity of the grown nanoparticles, they were applied individually and in conjunction with the third generation photosensitiser Fotolon (Chlorine e6) in the in vivo model of the normal liver of the Wister rat, and in the in vitro cancerous liver (HepG2) model both in the dark and under a variety of laser exposures (630 nm, Ultraviolet (UV) light). The localization of ZnO nanoparticles was observed by applying fluorescence spectroscopy on a 1 cm2 selected area of normal liver, whereas the in vitro cytotoxicity and reactive oxygen species (ROS) detection were carried out by calculating the loss in the cell viability of the hepatocellular model by applying a neutral red assay (NRA). Furthermore, a statistical analysis is carried out and it is ensured that the p value is less than 0.05. Thus, the current study has highlighted the potential for applying Zn/ZnO nanoparticles in photodynamic therapy that would lead to wider medical applications to improve the efficiency of cancer treatment and its biological aspect study.

scholarly journals Correction: Iqbal, S. et al. Empirical Modeling of Zn/ZnO Nanoparticles Decorated/Conjugated with Fotolon (Chlorine e6) Based Photodynamic Therapy towards Liver Cancer Treatment. Micromachines, 2019, 10, 60

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 262
Author(s):  
Seemab Iqbal ◽  
Muhammad Fakhar-e-Alam ◽  
M. Atif ◽  
Nasar Ahmed ◽  
Aqrab -ul-Ahmad ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Liver Cancer ◽  
Cancer Treatment ◽  
Zno Nanoparticles ◽  
Empirical Modeling ◽  
Chlorine E6 ◽  
Published Paper

In the published paper [...]

scholarly journals Novel Hybrid Compounds Containing Benzofuroxan and Aminothiazole Scaffolds: Synthesis and Evaluation of Their Anticancer Activity

2021 ◽  
Vol 22 (14) ◽  
pp. 7497
Author(s):  
Elena Chugunova ◽  
Gabriele Micheletti ◽  
Dario Telese ◽  
Carla Boga ◽  
Daut Islamov ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Normal Liver ◽  
Mitochondrial Pathway ◽  
Reaction Pathways ◽  
Aromatic Nucleophilic Substitution ◽  
X Ray ◽  
Hybrid Compounds ◽  
Aromatic Nucleophilic Substitution Reaction ◽  
Further Development

A series of novel hybrid compounds containing benzofuroxan and 2-aminothiazole moieties are synthesized via aromatic nucleophilic substitution reaction. Possible reaction pathways have been considered quantum-chemically, which allowed us to suggest the most probable products. The quantum chemical results have been proved by X-ray data on one compound belonging to the synthesized series. It was shown that the introduction of substituents to both the thiazole and amine moieties of the compounds under study strongly influences their UV/Vis spectra. Initial substances and obtained hybrid compounds have been tested in vitro as anticancer agents. Target compounds showed selectivity towards M-HeLa tumor cell lines and were found to be more active than starting benzofuroxan and aminothiazoles. Furthermore, they are considerably less toxic to normal liver cells compared to Тamoxifen. The mechanism of action of the studied compounds can be associated with the induction of apoptosis, which proceeds along the mitochondrial pathway. Thus, new hybrids of benzofuroxan are promising candidates for further development as anticancer agents.

scholarly journals Green Synthesis and Biomedical Applications of ZnO Nanoparticles: Role of PEGylated-ZnO Nanoparticles as Doxorubicin Drug Carrier against MDA-MB-231(TNBC) Cells Line

Crystals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 344
Author(s):  
Madiha Batool ◽  
Shazia Khurshid ◽  
Walid M. Daoush ◽  
Sabir Ali Siddique ◽  
Tariq Nadeem
Keyword(s):  
Breast Cancer ◽  
Electron Microscopy ◽  
Zno Nanoparticles ◽  
Drug Loading ◽  
Loading Capacity ◽  
X Ray Diffraction ◽  
Zno Nps ◽  
X Ray ◽  
Drug Loading Efficiency

The present study aimed to develop the synthesis of zinc oxide nanoparticles (ZnO-NPs) using the green method, with Aloe barbadensis leaf extract as a stabilizing and capping agent. In vitro antitumor cytotoxic activity, as well as the surface-functionalization of ZnO-NPs and their drug loading capacity against doxorubicin (DOX) and gemcitabine (GEM) drugs, were also studied. Morphological and structural properties of the produced ZnO-NPs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersion X-ray diffraction (EDX), UV-Vis spectrophotometry, Fourier-transform infrared analysis (FTIR), and X-ray diffraction (XRD). The prepared ZnO-NPs had a hexagonal shape and average particle size of 20–40 nm, with an absorption peak at 325 nm. The weight and atomic percentages of zinc (50.58% and 28.13%) and oxygen (26.71% and 60.71%) were also determined by EDAX (energy dispersive x-ray analysis) compositional analysis. The appearance of the FTIR peak at 3420 m–1 confirmed the synthesis of ZnO-NPs. The drug loading efficiency (LE) and loading capacity (LC) of unstabilized and PEGylated ZnO-NPs were determined by doxorubicin (DOX) and gemcitabine (GEM) drugs. DOX had superior LE 65% (650 mg/g) and higher LC 32% (320 mg/g) than GEM LE 30.5% (30 mg/g) and LC 16.25% (162 mg/g) on ZnO-NPs. Similar observation was observed in the case of PEG-ZnO-NPs, where DOX had enhanced LE 68% (680 mg/g) and LC 35% (350) mg/g in contrast to GEM, which had LE and LC values of 35% (350 mg/g) and 19% (190 mg/g), respectively. Therefore, DOX was chosen to encapsulate nanoparticles, along with the untreated nanoparticles, to check their in vitro antiproliferative potential against the triple-negative breast cancer (TNBC) cell line (MDA-MB-231) through the MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay. This drug delivery strategy implies that the PEGylated biogenically synthesized ZnO-NPs occupy an important position in chemotherapeutic drug loading efficiency and can improve the therapeutic techniques of triple breast cancer.

scholarly journals Nanoscintillator-Mediated X-ray Inducible Photodynamic Therapy for In Vivo Cancer Treatment

Nano Letters ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 2249-2256 ◽  
Author(s):  
Hongmin Chen ◽  
Geoffrey D. Wang ◽  
Yen-Jun Chuang ◽  
Zipeng Zhen ◽  
Xiaoyuan Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
X Ray

scholarly journals Multifunctional nanoplatforms as cascade-responsive drug-delivery carriers for effective synergistic chemo-photodynamic cancer treatment

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Fan Li ◽  
Yan Liang ◽  
Miaochen Wang ◽  
Xing Xu ◽  
Fen Zhao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Blood Circulation ◽  
The Novel ◽  
Hydrophobic Core ◽  
Cancer Suppression ◽  
532 Nm

AbstractSynergistic chemo-photodynamic therapy has garnered attention in the field of cancer treatment. Here, a pH cascade-responsive micellar nanoplatform with nucleus-targeted ability, for effective synergistic chemo-photodynamic cancer treatment, was fabricated. In this micellar nanoplatform, 5-(4-carboxyphenyl)-10,15,20-triphenylporphyrin (Por), a photodynamic therapy (PDT) agent was utilized for carrying the novel anticancer drug GNA002 to construct a hydrophobic core, and cyclic RGD peptide (cRGD)-modified polyethylene glycol (PEG) (cRGD-PEG) connected the cell-penetrating peptide hexaarginine (R6) through a pH-responsive hydrazone bond (cRGD-PEG-N = CH-R6) to serve as a hydrophilic shell for increasing blood circulation time. After passively accumulating in tumor sites, the self-assembled GNA002-loaded nanoparticles were actively internalized into cancer cells via the cRGD ligands. Once phagocytosed by lysosomes, the acidity-triggered detachment of the cRGD-PEG shell led to the formation of R6-coated secondary nanoparticles and subsequent R6-mediated nucleus-targeted drug delivery. Combined with GNA002-induced nucleus-specific chemotherapy, reactive oxygen species produced by Por under 532-nm laser irradiation achieved a potent synergistic chemo-photodynamic cancer treatment. Moreover, our in vitro and in vivo anticancer investigations revealed high cancer-suppression efficacy of this ideal multifunctional nanoplatform, indicating that it could be a promising candidate for synergistic anticancer therapy.

scholarly journals Effects of Orthodontic Bonding Containing TiO2 and ZnO Nanoparticles on Prevention of White Spot Lesions: an In Vitro Study

2021 ◽  
Vol 12 (1) ◽  
pp. 431-440
Keyword(s):  
Tio2 Nanoparticles ◽  
Zno Nanoparticles ◽  
Brain Heart Infusion ◽  
Negative Control ◽  
White Spot ◽  
Control Group ◽  
P Value ◽  
White Spot Lesions ◽  
White Spots

Demineralization is a common problem following orthodontic treatments. Today using antibacterial nanoparticles in preventing white spot lesions is being discussed. Given that ZnO and TiO2 nanoparticles have direct antibacterial known properties, this study aims to evaluate these nanoparticles' antibacterial effects in orthodontic bondings' composition on preventing white spot lesions. In this in vitro experimental study, 43 sound human premolar teeth were divided into five groups according to the adhesive utilized for bracket bonding: None group consisting of 12 teeth bonded with Transbond XT, TiO2 Group consisting of 12 teeth with Transbond XT and TiO2 nanoparticles, ZnO group consisting of 12 teeth with Transbond XT and ZnO nanoparticles, a positive control group consisting of 5 teeth without brackets and negative control groups consisting of 5 teeth in a sterile medium. All teeth were stored in a medium consisting of 1cc brain heart infusion (BHI) + sucrose 1%+ 0.5 McFarland Streptococcus mutans bacteria for 28 days. The medium was replaced every 48 hours. All the samples were examined every week for 4 weeks using DIAGNOdent and photography to detect white spots. The results of this study revealed that adding TiO2 and ZnO nanoparticles to Transbond XT bonding caused a decrease in enamel lesions occurrence and incidence of white spots (p value= 0.00). The results did not reveal significant differences between TiO2 and ZnO groups. Novel bonding agents containing TiO2 and ZnO nanoparticles represent promising candidates in combating enamel white spot lesions.

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