scholarly journals Seasonal Variations and Interspecific Differences in Metabolomes of Freshwater Fish Tissues: Quantitative Metabolomic Profiles of Lenses and Gills

Metabolites ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 264 ◽  
Author(s):  
Tsentalovich ◽  
Yanshole ◽  
Yanshole ◽  
Zelentsova ◽  
Melnikov ◽  
...  
Keyword(s):  
Feeding Activity ◽  
Rutilus Rutilus ◽  
Fish Health ◽  
Sander Lucioperca ◽  
Fish Tissues ◽  
Roach Rutilus Rutilus ◽  
Fish Feeding ◽  
Interspecific Differences ◽  
Pike Perch ◽  
First Time

This work represents the first comprehensive report on quantitative metabolomic composition of tissues of pike-perch (Sander lucioperca) and Siberian roach (Rutilus rutilus lacustris). The total of 68 most abundant metabolites are identified and quantified in the fish lenses and gills by the combination of LC-MS and NMR. It is shown that the concentrations of some compounds in the lens are much higher than that in the gills; that indicates the importance of these metabolites for the adaptation to the specific living conditions and maintaining the homeostasis of the fish lens. The lens metabolome undergoes significant seasonal changes due to the variations of dissolved oxygen level and fish feeding activity. The most season-affected metabolites are osmolytes and antioxidants, and the most affected metabolic pathway is the histidine pathway. In late autumn, the major lens osmolytes are N-acetyl-histidine and threonine phosphoethanolamine (Thr-PETA), while in winter the highest concentrations were observed for serine phosphoethanolamine (Ser-PETA) and myo-inositol. The presence of Thr-PETA and Ser-PETA in fish tissues and their role in cell osmotic protection are reported for the first time. The obtained concentrations can be used as baseline levels for studying the influence of environmental factors on fish health.

scholarly journals Most abundant metabolites in tissues of freshwater fish pike-perch (Sander lucioperca)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuri P. Tsentalovich ◽  
Ekaterina A. Zelentsova ◽  
Lyudmila V. Yanshole ◽  
Vadim V. Yanshole ◽  
Iliya M. Odud
Keyword(s):  
Freshwater Fish ◽  
Quantitative Data ◽  
Elevated Level ◽  
Metabolomic Analysis ◽  
Sander Lucioperca ◽  
Fish Tissues ◽  
Ocular Tissues ◽  
Pike Perch ◽  
Metabolite Concentrations ◽  
The Brain

Abstract Quantitative metabolomic analysis was performed for eleven tissues of freshwater fish pike-perch (Sander lucioperca), including gill, heart, liver, kidney, spleen, muscle, brain, milt, lens, aqueous (AH) and vitreous (VH) humors with the use of NMR spectroscopy. The absolute values of concentrations were determined for more than 65 most abundant metabolites in every tissue. It was found that from the metabolomic viewpoint, kidney and gill are the most similar tissues, while the metabolomic compositions of ocular tissues—lens, AH, and VH significantly differ from that of other tissues. The combinations of intracellular osmolytes and antioxidants are specific for every tissue. In particular, the concentration of antioxidant ovothiol A in the lens is much higher than in any other tissue, while the brain enjoys the elevated level of ascorbate. The most abundant osmolyte in the fish spleen, muscle, and heart is taurine, and in the brain, gill, and lens—myo-inositol. Other important osmolytes specific for particular tissues are N-acetyl-histidine, N-acetyl-aspartate, betaine, threonine-phosphoethanolamine, and serine-phosphoethanolamine. The quantitative data obtained in the present work can be used as the baseline metabolite concentrations in the fish tissues to evaluate the influence of seasonal, ecological and other factors on the fish metabolism.

Fish helminths in reservoirs of the Zarafshan river

2021 ◽  
Vol 4 ◽  
pp. 32-46
Author(s):  
A. Kuchboev ◽  
B. Soatov
Keyword(s):  
Carassius Auratus ◽  
Crucian Carp ◽  
Silver Carp ◽  
Rutilus Rutilus ◽  
Hypophthalmichthys Molitrix ◽  
Roach Rutilus Rutilus ◽  
Diplostomum Spathaceum ◽  
Bream Abramis Brama ◽  
Pike Perch ◽  
Stizostedion Lucioperca

The results of the study of the helminth fauna of 8 species fish of the lower reaches reservoirs of the Zarafshan River are presented - carp (Cyprinus carpio), crucian carp (Carassius auratus gibelio), roach (Rutilus rutilus), oriental bream (Abramis brama), pike perch (Stizostedion lucioperca), silver carp (Hypophthalmichthys molitrix), pike (Esox lucius), Turkestan barbel (Barbus capito conocephalus). 27 species of helminths were identified, including 12 species of cestodes (Caryophyllaeus laticeps Pallas, 1781; Caryophyllaeus fimbriceps Annenkova - Chlopina, 1919; Biacetabulum appendiculatum Szidat, 1937; Khawia sinensis Hsu, 1935; Bathybothrium rectangulum Bloch, 1782; Bothriocephalus opsariichthydis Yamaguti, 1934; Ligula intestinalis Linnaeus, 1758 larvae; Digramma interupta Rudolphi, 1810 larvae; Proteocephalus torulosus Batsch, 1786; Neogryporhynchus cheilanoristrotus Wedl, 1855 larvae; Gryporhynchus pusillus von Nordman, 1832 larvae; Valipora campylancristrota Rudolphi, 1819 larvae), 5 species of trematodes (Sanguinicola inermis Plehn, 1905; Orientocreadium siluri Bychowsky et Dubinina, 1954; Allocreadium isoporum Looss, 1894; Diplostomum spathaceum Rudolvae, 1832 lare; (Dioctophyme renale Goeze, 1782 larvae; Rhabdochona denudata Dujardin, 1845; Desmidocercella numidica Seurat, 1920 larvae; Camallanus truncatus Rudolphi, 1814; Camallanus lacustris Zoega, 1776; Philometra ovatachenko Zeder, 1803; 1779 larvae) and 2 species of acanthocephalus (Pomphorhynchus laevis Muller, 1776; Acantocephalus lucii Muller, 1776). This work includes the results of the research of the helminthes of the fish of waterbodies in the lowers of the Zarafshan River. As a result, the infection of fish with 27 species of helminthes, belonging to cestodes (12), trematodes (5), nematodes (8), and acanthocephalans (2) was established.

scholarly journals Розподілення метацеркаріїв Paracoenogonimusovatus (Trematoda, Cyathocotylidae) у м’язовій тканині прісноводних риб

2015 ◽  
pp. 95-98
Author(s):  
С. Л. Гончаров
Keyword(s):  
Rutilus Rutilus ◽  
Esox Lucius ◽  
Abramis Brama ◽  
Sander Lucioperca ◽  
Roach Rutilus Rutilus ◽  
Dorsal Fin ◽  
Scardinius Erythrophthalmus ◽  
Carassius Gibelio ◽  
Bream Abramis Brama ◽  
Minimum Number

Досліджено розподілення метацеркарії в трема-тод Paracoenogonimus ovatus (Katsurada, 1914) ум’язовій тканині популяції прісноводних риб, а саме:тарані (Rutilus rutilus), густери (Blicca bjoerkna), ляща(Abramis brama), карася (Carassius gibelio), червоно-пірки (Scardinius erythrophthalmus), щуки (Esox lucius),судака (Sander lucioperca). Показано, що максималь-на кількість метацеркаріїв P. ovatus локалізувалася уділянці спинних м’язів, а саме поблизу спинного плав-ця та становила 41,3 % від загальної кількості вияв-лених паразитів. Мінімальні показники кількості ме-тацеркаріїв трематоди спостерігали в ділянці аналь-ного плавця – 2,1 %.  The distribution of metacercariae of trematodes Paracoenogonimus ovatus (Katsurada, 1914) in the muscle tissue of the population of freshwater fish, such as: roach (Rutilus rutilus), silver bream (Blicca bjoerkna), bream (Abramis brama), crucian (Carassius gibelio), rudd (Scardinius erythrophthalmus), pike (Esox lucius), pikeperch (Sander lucioperca). It is shown that the maximum number of metacercariae P. ovatus was localized in the dorsal muscles, namely near the dorsal fin and amounted to 41,3 % of the total number of identified parasites. The minimum number of indicators fluke metacercariae is observed in the anal fin – 2,1 %.    

scholarly journals The Occurrence of Paracoenogonimus Ovatus (Trematoda, Cyathocotylidae) in Fish of Natural Reservoirs of Mykolaiv Region

2015 ◽  
Vol 49 (5) ◽  
pp. 421-426
Author(s):  
S. L. Goncharov ◽  
N. M. Soroka
Keyword(s):  
Infection Intensity ◽  
Rutilus Rutilus ◽  
Esox Lucius ◽  
Original Material ◽  
Abramis Brama ◽  
Sander Lucioperca ◽  
Roach Rutilus Rutilus ◽  
Scardinius Erythrophthalmus ◽  
Bream Abramis Brama ◽  
The City

The article presents the original findings of Paracoenogonimus ovatus (Katsurada,1914) in fish in the Southern Bug and the Inhul rivers in Mykolaiv Region in 2012-2014. The roach (Rutilus rutilus), silver bream (Blicca bjoerkna), bream (Abramis brama), crucian (Carassius gibelio), rudd (Scardinius erythrophthalmus), pike (Esox lucius), pikeperch (Sander lucioperca) were found to be infected with metacercariae of P. ovatus. Metacercariae of P. ovatus are described based on the original material. The highest prevalence of infection was observed in the roach, 82.3 %, the pike was infected in the less degree, with prevalence of 15.3 %. The infection intensity was highest in the roach, up to 247 specimens; that of the pikeperch was the lowest - 17 specimens. The highest occurrence of P. ovatus was observed in the parts of the river Southern Bug in Zhovtnevy, Mykolaiv, Novoodesky Districts, whereas in Voznesenky, Pervomaysky Discticts and in the city of Mykolaiv the occurrence was the lowest. In the area of the river Inhul the parasite was observed mostly in the waters of Bashtansky District in Mykolaiv Region.

Population Characteristics and Feeding of Roach Rutilus rutilus in Small Regulated River of the Kyiv Polissya

2020 ◽  
Vol 56 (1) ◽  
pp. 42-48
Author(s):  
Ye. A. Gupalo ◽  
I. I. Abramyuk ◽  
S. A. Afanasyev ◽  
O. V. Manturova ◽  
Ye. V. Savchenko
Keyword(s):  
Rutilus Rutilus ◽  
Population Characteristics ◽  
Regulated River ◽  
Roach Rutilus Rutilus
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