scholarly journals 1H NMR-Based Metabolomic Analysis of Sub-Lethal Perfluorooctane Sulfonate Exposure to the Earthworm, Eisenia fetida, in Soil

Metabolites ◽  
2013 ◽  
Vol 3 (3) ◽  
pp. 718-740 ◽  
Author(s):  
Brian Lankadurai ◽  
Vasile Furdui ◽  
Eric Reiner ◽  
André Simpson ◽  
Myrna Simpson
Keyword(s):  
1H Nmr ◽  
Eisenia Fetida ◽  
Perfluorooctane Sulfonate ◽  
Metabolomic Analysis

1H NMR metabolomics of Eisenia fetida responses after sub-lethal exposure to perfluorooctanoic acid and perfluorooctane sulfonate

2012 ◽  
Vol 9 (6) ◽  
pp. 502 ◽  
Author(s):  
Brian P. Lankadurai ◽  
André J. Simpson ◽  
Myrna J. Simpson
Keyword(s):  
1H Nmr ◽  
Eisenia Fetida ◽  
Principal Component ◽  
Atp Synthesis ◽  
Perfluorooctane Sulfonate ◽  
Perfluorooctanoic Acid ◽  
Acid Oxidation ◽  
Inner Mitochondrial Membrane ◽  
Contaminant Exposure ◽  
Pfos Exposure

Environmental contextPerfluoroalkyl acids are persistent environmental contaminants that are also found in soils. We use a metabolomics approach based on nuclear magnetic resonance analyses to investigate the responses of earthworms to exposure to sub-lethal levels of two perfluoroalkyl acids. The results indicate that this metabolomics approach is able to delineate the toxic mode of action of contaminants present at sub-lethal levels. AbstractMetabolomics entails the analysis of endogenous metabolites within organisms exposed to an external stressor such as an environmental contaminant. We utilised 1H NMR-based metabolomics to elucidate sub-lethal toxic mechanisms of Eisenia fetida earthworms after exposure to perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS). Earthworms were exposed to a range of concentrations of PFOA (6.25 to 50 μg cm–2) and PFOS (3.125 to 25 μg cm–2) by contact tests for 2 days. Earthworm tissues were extracted using a mixture of chloroform, methanol and water, and the polar fraction was analysed by 1H NMR spectroscopy. NMR-based metabolomic analysis revealed heightened E. fetida toxic responses with higher PFOA and PFOS exposure concentrations. Principal component analysis (PCA) exhibited significant separation between control and exposed earthworms along PC1 for all PFOA and PFOS exposure concentrations. Leucine, arginine, glutamate, maltose and adenosine triphosphate (ATP) are potential indicators of PFOA and PFOS exposure as these metabolite concentrations fluctuated with exposure. Our data also indicate that PFOA and PFOS exposure may increase fatty acid oxidation and interrupt ATP synthesis due to a disruption in the inner mitochondrial membrane structure. NMR-based metabolomics has promise as an insightful tool for elucidating the environmental toxicology of sub-lethal contaminant exposure.

Metabolomic analysis of Combretum lanceolatum plants interaction with Diaphorthe phaseolorum and Trichoderma spirale endophytic fungi through 1H NMR

2021 ◽  
Author(s):  
Jhuly Wellen Ferreira Lacerda ◽  
Katia Aparecida Siqueira ◽  
Leonardo Gomes de Vasconcelos ◽  
Barbara Sayuri Bellete ◽  
Evandro Luiz Dall’Oglio ◽  
...  
Keyword(s):  
1H Nmr ◽  
Endophytic Fungi ◽  
Metabolomic Analysis ◽  
Combretum Lanceolatum

Analysis of Eisenia fetida earthworm responses to sub-lethal C60 nanoparticle exposure using 1H-NMR based metabolomics

2015 ◽  
Vol 120 ◽  
pp. 48-58 ◽  
Author(s):  
Brian P. Lankadurai ◽  
Edward G. Nagato ◽  
André J. Simpson ◽  
Myrna J. Simpson
Keyword(s):  
1H Nmr ◽  
Eisenia Fetida ◽  
Nanoparticle Exposure

scholarly journals 1H-NMR-Based Metabolomic Analysis of Cerebrospinal Fluid From Adult Bilateral Moyamoya Disease

Medicine ◽  
2015 ◽  
Vol 94 (17) ◽  
pp. e629 ◽  
Author(s):  
Jin Pyeong Jeon ◽  
Taeho Yun ◽  
Xing Jin ◽  
Won-Sang Cho ◽  
Young-Je Son ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
1H Nmr ◽  
Moyamoya Disease ◽  
Metabolomic Analysis

1H NMR study of the effects of sample contamination in the metabolomic analysis of mouse urine

2007 ◽  
Vol 45 (1) ◽  
pp. 134-140 ◽  
Author(s):  
Haiwei Gu ◽  
Zhengzheng Pan ◽  
Chester Duda ◽  
Doug Mann ◽  
Candice Kissinger ◽  
...  
Keyword(s):  
1H Nmr ◽  
Metabolomic Analysis ◽  
Mouse Urine ◽  
Sample Contamination ◽  
Nmr Study

1H NMR Metabolomic analysis of coffee and tea samples for the quantative determination of the main constituents

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
J Schripsema ◽  
MD Vianna ◽  
MA Lemos ◽  
D Dagnino
Keyword(s):  
1H Nmr ◽  
Metabolomic Analysis
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