scholarly journals Metabolic Changes in Synaptosomes in an Animal Model of Schizophrenia Revealed by 1H and 1H,13C NMR Spectroscopy

Metabolites ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 79
Author(s):  
Brian R. Barnett ◽  
Fariba Fathi ◽  
Paulo Falco Cobra ◽  
Sue Y. Yi ◽  
Jacqueline M. Anderson ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Fusion Proteins ◽  
13C Nmr Spectroscopy ◽  
Nerve Terminals ◽  
Proof Of Concept ◽  
Wild Type ◽  
Limited Success ◽  
Concept Application

Synaptosomes are isolated nerve terminals that contain synaptic components, including neurotransmitters, metabolites, adhesion/fusion proteins, and nerve terminal receptors. The essential role of synaptosomes in neurotransmission has stimulated keen interest in understanding both their proteomic and metabolic composition. Mass spectrometric (MS) quantification of synaptosomes has illuminated their proteomic composition, but the determination of the metabolic composition by MS has been met with limited success. In this study, we report a proof-of-concept application of one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy for analyzing the metabolic composition of synaptosomes. We utilize this approach to compare the metabolic composition synaptosomes from a wild-type rat with that from a newly generated genetic rat model (Disc1 svΔ2), which qualitatively recapitulates clinically observed early DISC1 truncations associated with schizophrenia. This study demonstrates the feasibility of using NMR spectroscopy to identify and quantify metabolites within synaptosomal fractions.

scholarly journals Involvement of Very Short DNA Tandem Repeats and the Influence of the RAD52 Gene on the Occurrence of Deletions in Saccharomyces cerevisiae

Genetics ◽  
2000 ◽  
Vol 156 (2) ◽  
pp. 549-557 ◽  
Author(s):  
Anne J Welcker ◽  
Jacky de Montigny ◽  
Serge Potier ◽  
Jean-Luc Souciet
Keyword(s):  
Fusion Proteins ◽  
Tandem Repeats ◽  
Chromosomal Rearrangements ◽  
Reversion Rate ◽  
Wild Type ◽  
Recombination Mechanism ◽  
Nonhomologous Recombination ◽  
Deletion Rate ◽  
Dna Tandem Repeats

Abstract Chromosomal rearrangements, such as deletions, duplications, or Ty transposition, are rare events. We devised a method to select for such events as Ura+ revertants of a particular ura2 mutant. Among 133 Ura+ revertants, 14 were identified as the result of a deletion in URA2. Of seven classes of deletions, six had very short regions of identity at their junctions (from 7 to 13 bp long). This strongly suggests a nonhomologous recombination mechanism for the formation of these deletions. The total Ura+ reversion rate was increased 4.2-fold in a rad52Δ strain compared to the wild type, and the deletion rate was significantly increased. All the deletions selected in the rad52Δ context had microhomologies at their junctions. We propose two mechanisms to explain the occurrence of these deletions and discuss the role of microhomology stretches in the formation of fusion proteins.

Role of Barium(II) in the Determination of the Absolute Configuration of Chiral Amines by1H NMR Spectroscopy

2006 ◽  
Vol 71 (3) ◽  
pp. 1119-1130 ◽  
Author(s):  
Rosa García ◽  
José M. Seco ◽  
Saulo A. Vázquez ◽  
Emilio Quiñoá ◽  
Ricardo Riguera
Keyword(s):  
Nmr Spectroscopy ◽  
Absolute Configuration ◽  
Chiral Amines ◽  
The Absolute

Natural abundance 13C-NMR spectroscopy for the quantitative determination of fecal fat

2003 ◽  
Vol 36 (7) ◽  
pp. 505-510 ◽  
Author(s):  
P Kunz ◽  
B Künnecke ◽  
I Kunz ◽  
H Lengsfeld ◽  
M von Kienlin
Keyword(s):  
Nmr Spectroscopy ◽  
Quantitative Determination ◽  
13C Nmr ◽  
13C Nmr Spectroscopy ◽  
Natural Abundance ◽  
Fecal Fat

Determination of the structural configuration of cresol–novolak resins by 13C NMR spectroscopy

1982 ◽  
Vol 27 (9) ◽  
pp. 3449-3454 ◽  
Author(s):  
J. A. Carothers ◽  
E. Gipstein ◽  
W. W. Fleming ◽  
T. Tompkins
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr ◽  
13C Nmr Spectroscopy ◽  
Structural Configuration

scholarly journals Diagnostic and Treatment Approaches Involving Transthyretin in Amyloidogenic Diseases

2019 ◽  
Vol 20 (12) ◽  
pp. 2982 ◽  
Author(s):  
Gil Yong Park ◽  
Angelo Jamerlan ◽  
Kyu Hwan Shim ◽  
Seong Soo A. An
Keyword(s):  
Cerebrospinal Fluid ◽  
Genetic Mutations ◽  
Hormone Binding ◽  
Wild Type ◽  
Autonomic Motor ◽  
Hormone Binding Protein ◽  
The Brain ◽  
Tetrameric Form

Transthyretin (TTR) is a thyroid hormone-binding protein which transports thyroxine from the bloodstream to the brain. The structural stability of TTR in tetrameric form is crucial for maintaining its original functions in blood or cerebrospinal fluid (CSF). The altered structure of TTR due to genetic mutations or its deposits due to aggregation could cause several deadly diseases such as cardiomyopathy and neuropathy in autonomic, motor, and sensory systems. The early diagnoses for hereditary amyloid TTR with cardiomyopathy (ATTR-CM) and wild-type amyloid TTR (ATTRwt) amyloidosis, which result from amyloid TTR (ATTR) deposition, are difficult to distinguish due to the close similarities of symptoms. Thus, many researchers investigated the role of ATTR as a biomarker, especially its potential for differential diagnosis due to its varying pathogenic involvement in hereditary ATTR-CM and ATTRwt amyloidosis. As a result, the detection of ATTR became valuable in the diagnosis and determination of the best course of treatment for ATTR amyloidoses. Assessing the extent of ATTR deposition and genetic analysis could help in determining disease progression, and thus survival rate could be improved following the determination of the appropriate course of treatment for the patient. Here, the perspectives of ATTR in various diseases were presented.

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