scholarly journals In Vitro Bone Cell Behavior on Porous Titanium Samples: Influence of Porosity by Loose Sintering and Space Holder Techniques

Metals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 696 ◽  
Author(s):  
Ana Civantos ◽  
Mercè Giner ◽  
Paloma Trueba ◽  
Sheila Lascano ◽  
María-José Montoya-García ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Porous Scaffold ◽  
Porous Titanium ◽  
Bone Cell ◽  
Mechanical Reliability ◽  
Space Holder ◽  
Porous Implant ◽  
Balanced Optimization ◽  
Control Surfaces

A great variety of powder metallurgy techniques can produce biomimetic porous titanium structures with similar mechanical properties to host bone tissue. In this work, loose sintering and space holder techniques, two frequently used metallurgical techniques, are compared to evaluate the influences of porosity (content, size, morphology and wall roughness), mechanical properties (stiffness and yield strength) and in-vitro cellular responses (adhesion and proliferation of myoblasts and osteoblasts). These comparisons are made to achieve the best balance between biomechanical and bifunctional behavior of a partial porous implant for cortical bone replacement. Cell adhesion (filopodia presence) and spreading were promoted on both porous surfaces and fully dense substrates (non-porous control surfaces). Porous scaffold samples designed using 50 vol.% NaCl space holder technique had an improved bioactive response over those obtained with the loose sintering technique due to higher roughness and scaffold pore diameter. However, the presence of large and heterogeneous pores compromises the mechanical reliability of the implant. Considering both scenarios, the substrates obtained with 40 vol.% NH4HCO3 and pore size ranges between 100 and 200 μm provide a balanced optimization of size and strength to promote in-vitro osseointegration.

Space-holder effect on designing pore structure and determining mechanical properties in porous titanium

2014 ◽  
Vol 57 ◽  
pp. 712-718 ◽  
Author(s):  
Byounggab Lee ◽  
Taekyung Lee ◽  
Yongmoon Lee ◽  
Dong Jun Lee ◽  
Jiwon Jeong ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Pore Structure ◽  
Porous Titanium ◽  
Space Holder

scholarly journals Fabrication and In Vitro Evaluation of 3D Printed Porous Polyetherimide Scaffolds for Bone Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Xiongfeng Tang ◽  
Yanguo Qin ◽  
Xinyu Xu ◽  
Deming Guo ◽  
Wenli Ye ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Proliferation ◽  
Cell Adhesion ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
3D Printed

For bone tissue engineering, the porous scaffold should provide a biocompatible environment for cell adhesion, proliferation, and differentiation and match the mechanical properties of native bone tissue. In this work, we fabricated porous polyetherimide (PEI) scaffolds using a three-dimensional (3D) printing system, and the pore size was set as 800 μm. The morphology of 3D PEI scaffolds was characterized by the scanning electron microscope. To investigate the mechanical properties of the 3D PEI scaffold, the compressive mechanical test was performed via an electronic universal testing system. For the in vitro cell experiment, bone marrow stromal cells (BMSCs) were cultured on the surface of the 3D PEI scaffold and PEI slice, and cytotoxicity, cell adhesion, and cell proliferation were detected to verify their biocompatibility. Besides, the alkaline phosphatase staining and Alizarin Red staining were performed on the BMSCs of different samples to evaluate the osteogenic differentiation. Through these studies, we found that the 3D PEI scaffold showed an interconnected porous structure, which was consistent with the design. The elastic modulus of the 3D PEI scaffold (941.33 ± 65.26 MPa) falls in the range of modulus for the native cancellous bone. Moreover, the cell proliferation and morphology on the 3D PEI scaffold were better than those on the PEI slice, which revealed that the porous scaffold has good biocompatibility and that no toxic substances were produced during the progress of high-temperature 3D printing. The osteogenic differentiation level of the 3D PEI scaffold and PEI slice was equal and ordinary. All of these results suggest the 3D printed PEI scaffold would be a potential strategy for bone tissue engineering.

Mechanical Properties Improvement of Porous Titanium-Bioglass Nanocomposites by Mechanical Alloying

2013 ◽  
Vol 829 ◽  
pp. 319-323
Author(s):  
Saeed Riahi ◽  
Mohammad Rajabi ◽  
Sayed Mahmood Rabiee
Keyword(s):  
Mechanical Properties ◽  
Mechanical Alloying ◽  
Biomedical Application ◽  
Diffraction Method ◽  
Porous Titanium ◽  
Sintering Process ◽  
Compression Tests ◽  
X Ray Diffraction ◽  
Mechanically Alloyed ◽  
Space Holder

In this study, porous titanium-10 wt.% bioglass nanocomposites were fabricated by the combination of mechanical alloying and a space holder sintering process. The mixed powders were mechanically alloyed for 15 h. The blended Ti-Bioglass was mixed with 30 wt.% carbamide as a space holder. The mixtures were uniaxially pressed and finally, the green compacts sintered at 1150°C for 5 hours. The porous structures are characterized by X-ray diffraction method (XRD) and scanning electron microscopy (SEM). The mechanical properties were examined using micro hardness and compression tests. The investigation revealed that after 15 h of milling, the Bioglass dissolved in Ti lattice. Also, results show that nanostructured Ti-10 wt.% Bioglass with 31.5 nm crystallite size possess greater hardness compared to respective microcrystalline titanium and desirable compressive strength for using in biomedical application.

scholarly journals Characterization and in vitro assessment of three-dimensional extrusion Mg-Sr codoped SiO2-complexed porous microhydroxyapatite whisker scaffolds for biomedical engineering

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Chengyong Li ◽  
Tingting Yan ◽  
Zhenkai Lou ◽  
Zhimin Jiang ◽  
Zhi Shi ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Proliferation ◽  
Pore Size ◽  
Osteogenic Differentiation ◽  
Bone Repair ◽  
Porous Scaffold ◽  
Porous Scaffolds ◽  
Sprague Dawley ◽  
Active Elements

Abstract Background Large bone defects have always been a great challenge for orthopedic surgeons. The use of a good bone substitute obtained by bone tissue engineering (BTE) may be an effective treatment method. Artificial hydroxyapatite, a commonly used bone defect filler, is the main inorganic component of bones. Because of its high brittleness, fragility, and lack of osteogenic active elements, its application is limited. Therefore, its fragility should be reduced, its osteogenic activity should be improved, and a more suitable scaffold should be constructed. Methods In this study, a microhydroxyapatite whisker (mHAw) was developed, which was doped with the essential trace active elements Mg2+ and Sr2+ through a low-temperature sintering technique. After being formulated into a slurry, a bionic porous scaffold was manufactured by extrusion molding and freeze drying, and then SiO2 was used to improve the mechanical properties of the scaffold. The hydrophilicity, pore size, surface morphology, surface roughness, mechanical properties, and release rate of the osteogenic elements of the prepared scaffold were detected and analyzed. In in vitro experiments, Sprague–Dawley (SD) rat bone marrow mesenchymal stem cells (rBMSCs) were cultured on the scaffold to evaluate cytotoxicity, cell proliferation, spreading, and osteogenic differentiation. Results Four types of scaffolds were obtained: mHAw-SiO2 (SHA), Mg-doped mHAw-SiO2 (SMHA), Sr-doped mHAw-SiO2 (SSHA), and Mg-Sr codoped mHAw-SiO2 (SMSHA). SHA was the most hydrophilic (WCA 5°), while SMHA was the least (WCA 8°); SMHA had the smallest pore size (247.40 ± 23.66 μm), while SSHA had the largest (286.20 ± 19.04 μm); SHA had the smallest Young's modulus (122.43 ± 28.79 MPa), while SSHA had the largest (188.44 ± 47.89 MPa); and SHA had the smallest compressive strength (1.72 ± 0.29 MPa), while SMHA had the largest (2.47 ± 0.25 MPa). The osteogenic active elements Si, Mg, and Sr were evenly distributed and could be sustainably released from the scaffolds. None of the scaffolds had cytotoxicity. SMSHA had the highest supporting cell proliferation and spreading rate, and its ability to promote osteogenic differentiation of rBMSCs was also the strongest. Conclusions These composite porous scaffolds not only have acceptable physical and chemical properties suitable for BTE but also have higher osteogenic bioactivity and can possibly serve as potential bone repair materials.

Electrophoretic Deposition of PEEK/45S5 Bioactive Glass Coating on Porous Titanium Substrate: Influence of Processing Conditions and Porosity Parameters

2016 ◽  
Vol 704 ◽  
pp. 343-350 ◽  
Author(s):  
Yadir Torres ◽  
Carlos Romero ◽  
Qiang Chen ◽  
Gonzalo Pérez ◽  
José Antonio Rodríguez-Ortiz ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Electrophoretic Deposition ◽  
Colloidal Suspension ◽  
High Vacuum ◽  
Titanium Substrate ◽  
Porous Titanium ◽  
Processing Conditions ◽  
Space Holder ◽  
Cp Ti

Commercially pure titanium (cp Ti) is typically accepted as one of the best in vitro and in vivo bone replacement biomaterial, due to its excellent balance between biomechanical and biofunctional properties. In that context, the aim of this work is to prove the hypothesis of a simultaneous solution to certain specific limitations of cpTi, which can often compromise the reliability of implants: (i) stress-shielding phenomenon, and (ii) a deficient biointerface with bone, which reduces the osseointegration. Porous samples of cp Ti, grade IV, were obtained by space-holder technique (50 vol.% NH4HCO3, 800 MPa, at 1250 oC during 2h, under high vacuum), to produce a good balance between Young ́s Modulus and yield strength. Different types of porous samples were manufactured by considering different size particles ranges of NH4HCO3: 100-200μm, 250-355μm and 355-500μm. Afterwards, they were coated with a PEEK/45S5 bioactive glass composite by electrophoretic deposition, to be finally sintered at 350oC for 1h. The coatings homogeneity, infiltration efficiency, adhesion and cracking, were studied in order to establish correlations with processing conditions (time of deposition, applied voltage, composition, concentration and stability of the colloidal suspension). Detailed structural characterization of the coatings was performed (SEM and XRD), besides the contact angle and contact profilometry testing. Additional mechanical and chemical insights were achieved by evaluating both the tribo-mechanical (instrumented microindentation and micro-scratch testing) and electrochemical behaviors (potentiodynamic polarization and in vitro corrosion tests in SBF). All these results allowed us to determine the optimal balance of properties for a porous substrate (space holder of 250-355μm) with a coating obtained for 65 V, 2 min, 6 mm (distance between electrodes), 10 g/L bioactive glass and 20 g/l PEEK. The high adhesion estimated between the bioactive/biopolymer coatings and the porous titanium substrates (excellent infiltration) suggest that this new biocomposite is a good candidate for load-bearing applications.

scholarly journals In vitro and in vivo evaluations of mechanical properties, biocompatibility and osteogenic ability of sintered porous titanium alloy implant

RSC Advances ◽  
2018 ◽  
Vol 8 (64) ◽  
pp. 36512-36520 ◽  
Author(s):  
Ji Li ◽  
Zhongli Li ◽  
Ruiling Li ◽  
Yueyi Shi ◽  
Haoran Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Titanium Alloy ◽  
Bone Ingrowth ◽  
Porous Titanium ◽  
Porous Ti ◽  
Good Biocompatibility

The sintered porous Ti6Al4V with 75% porosity has optimal mechanical properties, good biocompatibility and osteogenic ability for more bone ingrowth.

Designing bioactive porous titanium interfaces to balance mechanical properties and in vitro cells behavior towards increased osseointegration

2019 ◽  
Vol 368 ◽  
pp. 162-174 ◽  
Author(s):  
Ana Civantos ◽  
Cristina Domínguez ◽  
Raisa Juliana Pino ◽  
Giulia Setti ◽  
Juan José Pavón ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Porous Titanium
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