scholarly journals Live Bacterial Vectors—A Promising DNA Vaccine Delivery System

2018 ◽  
Vol 6 (2) ◽  
pp. 27 ◽  
Author(s):  
Valentina Yurina
Keyword(s):  
Dna Vaccine ◽  
Delivery System ◽  
Vaccine Delivery ◽  
Vaccine Delivery System ◽  
Bacterial Vectors

scholarly journals Solid Lipid Nanoparticle Carrier Platform Containing Synthetic TLR4 Agonist Mediates Non-Viral DNA Vaccine Delivery

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 551
Author(s):  
Jasmine E. Francis ◽  
Ivana Skakic ◽  
Chaitali Dekiwadia ◽  
Ravi Shukla ◽  
Aya C. Taki ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Delivery System ◽  
Vaccine Delivery ◽  
Solid Lipid Nanoparticle ◽  
Viral Dna ◽  
Vaccine Delivery System ◽  
Solid Lipid ◽  
Monophosphoryl Lipid A ◽  
Lipid Nanoparticle

There is a growing demand for better delivery systems to improve the stability and efficacy of DNA vaccines. Here we report the synthesis of a non-viral DNA vaccine delivery system using a novel adjuvanted solid lipid nanoparticle (SLN-A) platform as a carrier for a DNA vaccine candidate encoding the Urease alpha (UreA) antigen from Helicobacter pylori. Cationic SLN-A particles containing monophosphoryl lipid A (adjuvant) were synthesised by a modified solvent-emulsification method and were investigated for their morphology, zeta potential and in vitro transfection capacity. Particles were found to bind plasmid DNA to form lipoplexes, which were characterised by electron microscopy, dynamic light scattering and fluorescence microscopy. Cellular uptake studies confirmed particle uptake within 3 h, and intracellular localisation within endosomal compartments. In vitro studies further confirmed the ability of SLN-A particles to stimulate expression of pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) in human macrophage-like Tohoku Hospital Pediatrics-1 (THP-1) cells. Lipoplexes were found to be biocompatible and could be efficiently transfected in murine immune cells for expression of recombinant H. pylori antigen Urease A, demonstrating their potential as a DNA vaccine delivery system.

A suicidal strain of Listeria monocytogenes is effective as a DNA vaccine delivery system for oral administration

Vaccine ◽  
2017 ◽  
Vol 35 (38) ◽  
pp. 5115-5122 ◽  
Author(s):  
Shubhra Sinha ◽  
Cheng-Yi Kuo ◽  
Joan K. Ho ◽  
Paul J. White ◽  
Jalal A. Jazayeri ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Oral Administration ◽  
Dna Vaccine ◽  
Delivery System ◽  
Vaccine Delivery ◽  
Vaccine Delivery System

Biodegradable microspheres as a vaccine delivery system

1991 ◽  
Vol 28 (3) ◽  
pp. 287-294 ◽  
Author(s):  
John H. Eldridge ◽  
Jay K. Staas ◽  
Jonathan A. Meulbroek ◽  
Jerry R. McGhee ◽  
Thomas R. Tice ◽  
...  
Keyword(s):  
Delivery System ◽  
Vaccine Delivery ◽  
Biodegradable Microspheres ◽  
Vaccine Delivery System

scholarly journals COVID-19: Vaccine Delivery System, Drug Repurposing and Application of Molecular Modeling Approach

2021 ◽  
Vol Volume 15 ◽  
pp. 3313-3330
Author(s):  
Soha R Abd El Hadi ◽  
Esmat E Zien El-Deen ◽  
Mostafa M Bahaa ◽  
Abdelfattah A Sadakah ◽  
Heba A Yassin
Keyword(s):  
Molecular Modeling ◽  
Delivery System ◽  
Vaccine Delivery ◽  
Drug Repurposing ◽  
Vaccine Delivery System ◽  
Modeling Approach

A Potent Vaccine Delivery System

BIO-PROTOCOL ◽  
2021 ◽  
Vol 11 (7) ◽  
Author(s):  
Guangzu Zhao ◽  
Armira Azuar ◽  
Istvan Toth ◽  
Mariusz Skwarczynski
Keyword(s):  
Delivery System ◽  
Vaccine Delivery ◽  
Vaccine Delivery System
Sign in / Sign up

Export Citation Format

Share Document