scholarly journals Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta) by Improvement of Culture Conditions and Random Mutagenesis

Marine Drugs ◽  
2011 ◽  
Vol 9 (9) ◽  
pp. 1607-1624 ◽  
Author(s):  
Baldo F. Cordero ◽  
Irina Obraztsova ◽  
Inmaculada Couso ◽  
Rosa Leon ◽  
Maria Angeles Vargas ◽  
...  
Keyword(s):  
Random Mutagenesis ◽  
Culture Conditions ◽  
Chlorella Sorokiniana ◽  
Lutein Production

Central composite design for simultaneously optimizing biomass and lutein production by a mixotrophic Chlorella sorokiniana TH01

2021 ◽  
pp. 108231
Author(s):  
Cam Van T. Do ◽  
Nham Tuat T. Nguyen ◽  
Mai Huong T. Pham ◽  
Thanh Yen T. Pham ◽  
Van Gioi Ngo ◽  
...  
Keyword(s):  
Central Composite Design ◽  
Chlorella Sorokiniana ◽  
Central Composite ◽  
Lutein Production

scholarly journals Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 324 ◽  
Author(s):  
Juan-Luis Fuentes ◽  
Zaida Montero ◽  
María Cuaresma ◽  
Mari-Carmen Ruiz-Domínguez ◽  
Benito Mogedas ◽  
...  
Keyword(s):  
Biomass Production ◽  
Large Scale ◽  
Culture Conditions ◽  
High Concentration ◽  
Ph Values ◽  
Lutein Content ◽  
Acidic Waters ◽  
Large Scale Cultivation ◽  
Rate Limiting ◽  
Lutein Production

The large-scale biomass production is an essential step in the biotechnological applications of microalgae. Coccomyxa onubensis is an acidophilic microalga isolated from the highly acidic waters of Río Tinto (province of Huelva, Spain) and has been shown to accumulate a high concentration of lutein (9.7 mg g−1dw), a valuable antioxidant, when grown at laboratory-scale. A productivity of 0.14 g L−1 d−1 was obtained by growing the microalga under outdoor conditions in an 800 L tubular photobioreactor. The results show a stable biomass production for at least one month and with a lutein content of 10 mg g−1dw, at pH values in the range 2.5–3.0 and temperature in the range 10–25 °C. Culture density, temperature, and CO2 availability in highly acidic medium are rate-limiting conditions for the microalgal growth. These aspects are discussed in this paper in order to improve the outdoor culture conditions for competitive applications of C. onubensis.

A highly efficient two-stage cultivation strategy for lutein production using heterotrophic culture of Chlorella sorokiniana MB-1-M12

2018 ◽  
Vol 253 ◽  
pp. 141-147 ◽  
Author(s):  
Chun-Yen Chen ◽  
I-Chia Lu ◽  
Dillirani Nagarajan ◽  
Chien-Hsiang Chang ◽  
I-Son Ng ◽  
...  
Keyword(s):  
Chlorella Sorokiniana ◽  
Heterotrophic Culture ◽  
Two Stage ◽  
Highly Efficient ◽  
Cultivation Strategy ◽  
Lutein Production

scholarly journals Optimization of Cultivation Condition of Newly Isolated Strain Chlorella Sorokiniana pa.91 for CO2 Bio-Fixation and Nutrients Removal From Real Municipal Wastewater: Impact of Temperature and Light Intensity

2021 ◽  
Author(s):  
Poone Yaqoubnejad ◽  
Hassan Aminirad ◽  
Mohsen Taghavijeloudar
Keyword(s):  
Municipal Wastewater ◽  
Removal Rate ◽  
High Capacity ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Batch Cultivation ◽  
Culture Conditions ◽  
Chlorella Sorokiniana ◽  
Synthetic Wastewater ◽  
Cultivation Condition

Abstract The cultivation conditions of a newly isolated strain Chlorella sorokiniana pa.91 were optimized for the first time by performing sixty batch cultivation experiments at various temperatures (20, 25, 30 and 35 °C) and light intensities (1000, 3000, 4000, 5000 and 7000 Lux) in three different culture mediums of BG-11, real settled municipal wastewater (RMWW) and synthetic wastewater (SWW). Additionally, to evaluate the capability of C. sorokiniana pa.91 in CO2 bio-fixation and wastewater treatment, the microalgae was cultivated in a flat-plate photobioreactor (CO2 = 16% and 0.6 vvm aeration) under the optimal condition. The optimization results suggested that at the culture conditions of 30 °C, 4000 Lux and RMWW (COD 211 mgL-1) microalgae had the best performance in growth and biomass productivity. Maximum biomass concentration and productivity of 3.21 gL-1 and 0.31 gL-1d-1were achieved, respectively, by cultivation of C. sorokiniana pa.91 in the photobioreactor under the optimized condition. Experimental results showed that C. sorokiniana pa.91 has a high capacity of CO2 bio-fixation (0.59 mgL-1d-1) and CO2 removal rate (35.6 %). Moreover, using C. sorokiniana pa.91 could efficiently remove 74% of NH3, 93% of NO3-, 83% of PO4-3 and 76% of COD from real municipal wastewater after eight days of cultivation in the photobioreactor.

scholarly journals Optimized and Automated Protocols for High-Throughput Screening of Amylosucrase Libraries

2007 ◽  
Vol 12 (5) ◽  
pp. 715-723 ◽  
Author(s):  
Stéphane Emond ◽  
Gabrielle Potocki-Véronèse ◽  
Philippe Mondon ◽  
Khalil Bouayadi ◽  
Hakim Kharrat ◽  
...  
Keyword(s):  
Organic Solvents ◽  
High Throughput ◽  
High Throughput Screening ◽  
General Procedure ◽  
Random Mutagenesis ◽  
Culture Conditions ◽  
Screening Assay ◽  
High Production Rate ◽  
Automated Screening

This article describes the design and validation of a general procedure for the high-throughput isolation of amylosucrase variants displaying higher thermostability or increased resistance to organic solvents. This procedure consists of 2 successive steps: an in vivo selection that eliminates inactive variants followed by automated screening of active variants to isolate mutants displaying enhanced features. The authors chose an Escherichia coli expression vector, allowing a high production rate of the recombinant enzyme in miniaturized culture conditions. The screening assay was validated by minimizing variability for various parameters of the protocol, especially bacterial growth and protein production in cultures in 96-well microplates. Recombinant amylosucrase production was normalized by decreasing the coefficient of variance from 27% to 12.5%. Selective screening conditions were defined to select variants displaying higher thermostability or increased resistance to organic solvents. A first-generation amylosucrase variant library, constructed by random mutagenesis, was subjected to this procedure, yielding a mutant displaying a 25-fold increased stability at 50 °C compared to the parental wild-type enzyme. ( Journal of Biomolecular Screening 2007:715-723)

Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies

2019 ◽  
Vol 278 ◽  
pp. 17-25 ◽  
Author(s):  
Jih-Heng Chen ◽  
Chun-Yen Chen ◽  
Tomohisa Hasunuma ◽  
Akihiko Kondo ◽  
Chien-Hsiang Chang ◽  
...  
Keyword(s):  
Chlorella Sorokiniana ◽  
Mixotrophic Cultivation ◽  
Lutein Production

scholarly journals Amino acids and acylcarnitine production by Chlorella vulgaris and Chlorella sorokiniana microalgae from wastewater culture

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7977
Author(s):  
Juan M. Ballesteros-Torres ◽  
Luis Samaniego-Moreno ◽  
Ricardo Gomez-Flores ◽  
Reyes S. Tamez-Guerra ◽  
Cristina Rodríguez-Padilla ◽  
...  
Keyword(s):  
Amino Acids ◽  
Chlorella Vulgaris ◽  
Essential Amino Acids ◽  
Culture Conditions ◽  
Chlorella Sorokiniana ◽  
Liquid Chromatograph ◽  
Photosynthetic Microorganisms ◽  
High Concentrations ◽  
Growing Conditions ◽  
C4 Production

Background Microalgae are a widely distributed group of prokaryotic and eukaryotic photosynthetic microorganisms that use a number of substances present in wastewater to produce a variety of biotechnological and nutritional biomolecules. Methods Production ofamino acids and acylcarnitine by Chlorella vulgaris and Chlorella sorokiniana was determined after 13 d of culture in wastewater, under various culture conditions. Wastewater was collected from “La Encantada” stream, located in Saltillo, Coahuila, Mexico. Microalgae was cultured at 23°C and natural day light, including the use of the following conditions: (1) extra light (12:12 light:dark cycles, 1,380 lumens), (2) agitation (130 rpm), and (3) both conditions, until exponential phase. Supernatant products were then analyzed by liquid chromatograph coupled to mass spectrometry. In addition, metabolomic profiles related to growing conditions were evaluated. Results Amino acids and acylcarnitine production by C. sorokiniana and C. vulgaris resulted in higher Ala and Leu concentrations by C. vulgaris compared with control, where control produced Gly and Pro in higher amounts compared with C. sorokiniana. Tyr, Phe, Val, and Cit were detected in lower amounts under light and shaking culture conditions. High concentrations of C0 acylcarnitines were produced by both microalgae compared with control, where C. sorokiniana production was independent of culture conditions, whereas C. vulgaris one was stimulated by shaking. C4 production was higher by C. sorokiniana compared with control. Furthermore, C4, C6DC, C14:1, C14:2, and C18:1OH production by microalga was low in all culture conditions. Conclusion Microalgae produced essential amino acids and nutritionally important carnitines from wastewater. In addition, C. sorokiniana biomass has higher potential as animal nutrient supplement, as compared with that of C. vulgaris.

Pilot-scale cultivation of Chlorella sorokiniana FZU60 with a mixotrophy/photoautotrophy two-stage strategy for efficient lutein production

2020 ◽  
Vol 314 ◽  
pp. 123767
Author(s):  
Youping Xie ◽  
Jun Li ◽  
Shih-Hsin Ho ◽  
Ruijuan Ma ◽  
Xinguo Shi ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Chlorella Sorokiniana ◽  
Two Stage ◽  
Lutein Production
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