Leucine Aminopeptidase, β-Glucosidase and Alkaline Phosphatase Activity Rates and Their Significance in Nutrient Cycles in Some Coastal Mediterranean Sites

Gabriella Caruso

doi:10.3390/md8040916

Leucine Aminopeptidase, β-Glucosidase and Alkaline Phosphatase Activity Rates and Their Significance in Nutrient Cycles in Some Coastal Mediterranean Sites

Marine Drugs ◽

10.3390/md8040916 ◽

2010 ◽

Vol 8 (4) ◽

pp. 916-940 ◽

Cited By ~ 30

Author(s):

Gabriella Caruso

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Leucine Aminopeptidase ◽

Nutrient Cycles

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Isozyme characteristics of Caloscyphafulgens infested and pathogen-free spruce seed samples and use of alkaline phosphatase activity for qualitative and quantitative disease incidence assays

Canadian Journal of Forest Research ◽

10.1139/x81-027 ◽

1981 ◽

Vol 11 (2) ◽

pp. 201-206

Author(s):

Jack R. Sutherland ◽

Ute Rink ◽

E. E. McMullan ◽

T. A. D. Woods

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Gel Electrophoresis ◽

Leucine Aminopeptidase ◽

Disease Incidence ◽

Prediction Equation ◽

Qualitative And Quantitative ◽

Phosphogluconate Dehydrogenase ◽

Disease Free

Extracts of samples from Caloscyphafulgens infested and noninfested Sitka spruce (Piceasitchensis (Bong.) Carr.) seed lots and diseased and healthy seeds were separated by electrophoresis on polyacrylamide gels and stained for esterase (EC 3.1.1.1), leucine aminopeptidase (EC 3.4.1.1), acid phosphatase (EC 3.1.3.2), alkaline phosphatase (EC 3.1.3.1), glucose-6-phosphatase (EC 3.1.3.9), malate dehydrogenase (EC 1.1.1.37), isocitrate dehydrogenase (EC 1.1.1.42), 6-phosphogluconate dehydrogenase (EC 1.1.1.43) and alcohol dehydrogenase (EC 1.1.1.1). Several differences were detected between the isozyme patterns of disease-free and infested samples, but the main difference was the latter's high alkaline phosphatase activity. By using gel electrophoresis, (i) a qualitative analysis was developed, based on the presence or absence of alkaline phosphatase, to distinguish infested from disease-free seed lots, and (ii) the high alkaline phosphatase activity of infested samples was determined to be of pathogenic origin. By determining the alkaline phosphatase activity of samples with known numbers of diseased seeds, a prediction equation was derived relating enzyme activity and disease incidence. Correlation analyses showed significant (P = 0.01) correlations between disease incidence estimates obtained by this technique and plating of surface-sterilized seeds on water agar.

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LEUCOCYTE METABOLISM IN PREGNANCY

Acta Endocrinologica ◽

10.1530/acta.0.xxxv0575 ◽

1960 ◽

Vol XXXV (IV) ◽

pp. 575-584 ◽

Cited By ~ 2

Author(s):

C. Borel ◽

J. Frei ◽

A. Vannotti

Keyword(s):

Alkaline Phosphatase ◽

Oxygen Consumption ◽

Pregnant Women ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Lactate Production ◽

Glucose Consumption ◽

In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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