scholarly journals Evaluation of Aqueous Biphasic Electrophoresis System Based on Halide-Free Ionic Liquids for Direct Recovery of Keratinase

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 463
Author(s):  
Phei Er Kee ◽  
Hip Seng Yim ◽  
Akihiko Kondo ◽  
John Chi-Wei Lan ◽  
Hui Suan Ng
Keyword(s):  
Electric Fields ◽  
Large Scale ◽  
Separation Efficiency ◽  
Operation Time ◽  
Recovery Process ◽  
Biphasic System ◽  
Alkyl Sulphate ◽  
Electrophoresis System ◽  
Aqueous Biphasic ◽  
Optimum Partition

Aqueous biphasic electrophoresis system (ABES) incorporates electric fields into the biphasic system to separate the target biomolecules from crude feedstock. Ionic liquid (IL) is regarded as an excellent candidate as the phase-forming components for ABES because of the great electrical conductivity, which can promote the electromigration of biomolecules in ABES, and thereby enhances the separation efficiency of the target biomolecules from crude feedstock. The application of electric fields to the conventional biphasic system expedites the phase settling time of the biphasic system, which eases the subsequent scaling-up steps and reduces the overall processing time of the recovery process. Alkyl sulphate-based IL is a green and economical halide-free surfactant when compared to the other halide-containing IL. The feasibility of halide-free IL-based ABES to recover Kytococcus sedentarius TWHK01 keratinase was studied. Optimum partition coefficient (Ke = 7.53 ± 0.35) and yield (YT = 80.36% ± 0.71) were recorded with IL-ABES comprised of 15.0% (w/w) [EMIM][ESO4], 20.0% (w/w) sodium carbonate and 15% (w/w) crude feedstock. Selectivity (S) of 5.75 ± 0.27 was obtained with the IL-ABES operated at operation time of 5 min with 10 V voltage supplied. Halide-free IL is proven to be a potential phase-forming component of IL-ABES for large-scale recovery of keratinase.

scholarly journals Development and evaluation of a monoclonal antibody-based competitive ELISA for the detection of antibodies against H7 avian influenza virus

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Yuan Li ◽  
Hongliu Ye ◽  
Meng Liu ◽  
Suquan Song ◽  
Jin Chen ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Large Scale ◽  
Operation Time ◽  
Reference Method ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Concentration

Abstract Background H7 subtype avian influenza has caused great concern in the global poultry industry and public health. The conventional serological subtype-specific diagnostics is implemented by hemagglutination inhibition (HI) assay despite lengthy operation time. In this study, an efficient, rapid and high-throughput competitive enzyme-linked immunosorbent assay (cELISA) was developed for detection of antibodies against H7 avian influenza virus (AIV) based on a novel monoclonal antibody specific to the hemagglutinin (HA) protein of H7 AIV. Results The reaction parameters including antigen coating concentration, monoclonal antibody concentration and serum dilution ratio were optimized for H7 antibody detection. The specificity of the cELISA was tested using antisera against H1 ~ H9, H11 ~ H14 AIVs and other avian viruses. The selected cut-off values of inhibition rates for chicken, duck and peacock sera were 30.11, 26.85 and 45.66% by receiver-operating characteristic (ROC) curve analysis, respectively. With HI test as the reference method, the minimum detection limits for chicken, duck and peacock positive serum reached 20, 21 and 2− 1 HI titer, respectively. Compared to HI test, the diagnostic accuracy reached 100, 98.6, and 99.3% for chicken, duck and peacock by testing a total of 400 clinical serum samples, respectively. Conclusions In summary, the cELISA assay developed in this study provided a reliable, specific, sensitive and species-independent serological technique for rapid detection of H7 antibody, which was applicable for large-scale serological surveillance and vaccination efficacy evaluation programs.

Integrated extractive disruption of Gordonia terrae cells with direct recovery of carotenoids using alcohol/salt aqueous biphasic system

2019 ◽  
Vol 223 ◽  
pp. 107-112 ◽  
Author(s):  
Kai-Wen Lee ◽  
Chee Wun How ◽  
Lei Chen ◽  
Po Ting Chen ◽  
John Chi-Wei Lan ◽  
...  
Keyword(s):  
Biphasic System ◽  
Aqueous Biphasic System ◽  
Aqueous Biphasic

scholarly journals ThermoEnergy Ammonia Recovery Process for Municipal and Agricultural Wastes

2001 ◽  
Vol 1 ◽  
pp. 908-913 ◽  
Author(s):  
Alex G. Fassbender
Keyword(s):  
New York ◽  
Ammonium Sulfate ◽  
Large Scale ◽  
Biogas Production ◽  
Water Discharge ◽  
Treatment Plant ◽  
Recovery Process ◽  
Discharge Process ◽  
Salt Crystal ◽  
Ammonia Recovery

The Ammonia Recovery Process (ARP) is an award-winning, low-cost, environmentally responsible method of recovering nitrogen, in the form of ammonia, from various dilute waste streams and converting it into concentrated ammonium sulfate. The ThermoEnergy Biogas System utilizes the new chemisorption-based ARP to recover ammonia from anaerobically digested wastes. The process provides for optimal biogas production and significantly reduced nitrogen levels in the treated water discharge. Process flows for the ammonia recovery and ThermoEnergy biogas processes are presented and discussed. A comparison with other techniques such as biological nitrogen removal is made. The ARP technology uses reversible chemisorption and double salt crystal precipitation to recover and concentrate the ammonia. The ARP technology was successfully proven in a recent large-scale field demonstration at New York City’s Oakwood Beach Wastewater Treatment Plant, located on Staten Island. This project was a joint effort with Foster Wheeler Environmental Corporation, the Civil Engineering Research Foundation, and New York City Department of Environmental Protection. Independent validated plant data show that ARP consistently recovers up to 99.9% of the ammonia from the city’s centrate waste stream (derived from dewatering of sewage sludge), as ammonium sulfate. ARP technology can reduce the nitrogen (ammonia) discharged daily into local bodies of water by municipalities, concentrated animal farming operations, and industry. Recent advances to ARP enhance its performance and economic competitiveness in comparison to stripping or ammonia destruction technologies.

Optimization of Restoration Paths of Power System after Blackout

2013 ◽  
Vol 347-350 ◽  
pp. 1467-1472
Author(s):  
Wen Wei Huang ◽  
Gang Yao ◽  
Xiao Yan Qiu ◽  
Nian Liu ◽  
Guang Tang Chen
Keyword(s):  
Power System ◽  
Transmission Lines ◽  
Reactive Power ◽  
Minimum Spanning Tree ◽  
Operation Time ◽  
Combinatorial Problem ◽  
Weather Conditions ◽  
Recovery Process ◽  
Point Of View ◽  
Improved Genetic Algorithm

Optimization of restoration paths of power system after blackout is a multi-stage, multi-target, multi-variable combinatorial problem in the power system restoration. This paper presents a reasonable model and effectually method. The proposed model is considered as a typical partial minimum spanning tree problem from the mathematical point of view which considering all kinds of constraints. Improved data envelopment analysis (DEA) was used to get the weight which considering line charging reactive power, weather conditions, operation time and betweenness of transmission lines. The improved genetic algorithm method is employed to solve this problem. Finally, an example is given which proves the strategy of the line restoration can effectively handle the uncertainty of the system recovery process, to guarantee the system successfully restored after the catastrophic accidents.

Separation of Iodide Complex of Cadmium (II) in Propyl-Alcohol Ammonium Sulfate Aqueous Biphasic System

2014 ◽  
Vol 955-959 ◽  
pp. 2200-2204
Author(s):  
Guo Kui Zeng ◽  
Gui Hua Li
Keyword(s):  
Ammonium Sulfate ◽  
Ion Pair ◽  
Pair Formation ◽  
Experimental Results ◽  
Biphasic System ◽  
Extraction System ◽  
Propyl Alcohol ◽  
Aqueous Biphasic System ◽  
Aqueous Biphasic ◽  
Iodide Complex

The distribution behaviors of iodide complex of Cd (II) in the propyl-alcohol ammonium sulfate aqueous biphasic extraction system was experimentally investigated in this work. Cd (II) was found to be extracted from the propyl-alcohol phase due to its ion pair formation as CdI42-(PrOH2+)2 in the presence of NH4I and in HCL, and the associated extraction conditions were optimized. The experimental results indicated that cadmium can be efficiently extracted by the method, and an extraction rate of 99.1% for cadmium can be achieved under optimizing conditions.

Aqueous Biphasic System Containing Long Chain Anion-Functionalized Ionic Liquids for High-Performance Extraction

2015 ◽  
Vol 3 (12) ◽  
pp. 3365-3372 ◽  
Author(s):  
Yuanbang Xie ◽  
Huabin Xing ◽  
Qiwei Yang ◽  
Zongbi Bao ◽  
Baogen Su ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
High Performance ◽  
Biphasic System ◽  
Aqueous Biphasic System ◽  
Aqueous Biphasic ◽  
Chain Anion ◽  
Long Chain
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