scholarly journals Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 347
Author(s):  
Shuang-Yi Zhang ◽  
Guo-Xu Zhao ◽  
Shi-Kun Suo ◽  
Yu-Mei Wang ◽  
Chang-Feng Chi ◽  
...  
Keyword(s):  
Antarctic Krill ◽  
Reducing Power ◽  
Euphausia Superba ◽  
Antioxidant Peptides ◽  
Antioxidant Agents ◽  
Health Products ◽  
Protein Sequencer ◽  
Lipid Peroxidation Inhibition ◽  
Inhibition Ability ◽  
Antarctic Krill Euphausia Superba

For utilizing the largest source of marine proteins, Antarctic krill (Euphausia superba) proteins were defatted and hydrolyzed separately using pepsin, alcalase, papain, trypsin, and netrase, and alcalase hydrolysate (EPAH) showed the highest DPPH radical (DPPH·) and hydroxyl radical (HO·) scavenging activity among five hydrolysates. Using ultrafiltration and chromatography methods, fifteen antioxidant peptides were purified from EPAH and identified as Asn-Gln-Met (NQM), Trp-Phe-Pro-Met (WFPM), Gln-Asn-Pro-Thr (QNPT), Tyr-Met-Asn-Phe (YMNF), Ser-Gly-Pro-Ala (SGPA), Ser-Leu-Pro-Tyr (SLPY), Gln-Tyr-Pro-Pro-Met-Gln-Tyr (QYPPMQY), Glu-Tyr-Glu-Ala (EYEA), Asn-Trp-Asp-Asp-Met-Arg-Ile-Val-Ala-Val (NWDDMRIVAV), Trp-Asp-Asp-Met-Glu-Arg-Leu-Val-Met-Ile (WDDMERLVMI), Asn-Trp-Asp-Asp-Met-Glu-Pro-Ser-Phe (NWD-DMEPSF), Asn-Gly-Pro-Asp-Pro-Arg-Pro-Ser-Gln-Gln (NGPDPRPSQQ), Ala-Phe-Leu-Trp-Asn (AFLWA), Asn-Val-Pro-Asp-Met (NVPDM), and Thr-Phe-Pro-Ile-Tyr-Asp-Tyr-Pro-Gln (TFPIYDPQ), respectively, using a protein sequencer and ESI/MS. Among fifteen antioxidant peptides, SLPY, QYPPMQY and EYEA showed the highest scavenging activities on DPPH· (EC50 values of 1.18 ± 0.036, 1.547 ± 0.150, and 1.372 ± 0.274 mg/mL, respectively), HO· (EC50 values of 0.826 ± 0.027, 1.022 ± 0.058, and 0.946 ± 0.011 mg/mL, respectively), and superoxide anion radical (EC50 values of 0.789 ± 0.079, 0.913 ± 0.007, and 0.793 ± 0.056 mg/mL, respectively). Moreover, SLPY, QYPPMQY and EYEA showed strong reducing power, protective capability against H2O2-damaged plasmid DNA, and lipid peroxidation inhibition ability. Furthermore, SLPY, QYPPMQY, and EYEA had high stability under temperatures lower than 80 °C, pH values ranged from 6–8, and simulated GI digestion for 180 min. The results showed that fifteen antioxidant peptides from alcalase hydrolysate of Antarctic krill proteins, especially SLPY, QYPPMQY and EYEA, might serve as effective antioxidant agents applied in food and health products.

scholarly journals Identification and Active Evaluation of Antioxidant Peptides from Protein Hydrolysates of Skipjack Tuna (Katsuwonus pelamis) Head

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 318 ◽  
Author(s):  
Lun Zhang ◽  
Guo-Xu Zhao ◽  
Yu-Qin Zhao ◽  
Yi-Ting Qiu ◽  
Chang-Feng Chi ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Ionization Mass ◽  
Skipjack Tuna ◽  
Antioxidant Peptides ◽  
Katsuwonus Pelamis ◽  
System Method ◽  
Antioxidant Agents ◽  
Protein Sequencer

For the full use of fish by-products to produce antioxidant peptides, skipjack tuna (Katsuwonus pelamis) heads generated during can processing were defatted and hydrolyzed using the in vitro gastrointestinal (GI) digestion (pepsin–trypsin system) method and six antioxidant peptides (P1 to P6) were purified from the head hydrolysate (KPH) using ultrafiltration and serial chromatography methods. Six isolated peptides (P1 to P6) were identified as Val-Glu-Glu (VEE, P1), Trp-Met-Phe-Asp-Trp (WMFDW, P2), Asp-Ala-Gly-Pro-Tyr-Gly-Pro-Ile (DAGPYGPI, P3), Trp-Met-Gly-Pro-Tyr (WMGPY, P4), Glu-Arg-Gly-Pro-Leu-Gly-Pro-His (ERGPLGPH, P5), and Glu-Met- Gly-Pro-Ala (EMGPA, P6), respectively, using a protein sequencer and electrospray ionization-mass spectrometer. Among skipjack tuna head hydrolysates, fractions, and six isolated peptides (P1 to P6), WMFDW (P2), WMGPY (P4), and EMGPA (P6) showed the highest radical scavenging activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH) (EC50 values of 0.31, 0.33, and 0.46 mg/mL for WMFDW, WMGPY, and EMGPA, respectively), hydroxyl (EC50 values of 0.30, 0.43, and 0.52 mg/mL for WMFDW, WMGPY, and EMGPA, respectively), and superoxide anion (EC50 values of 0.56, 0.38, and 0.71 mg/mL for WMFDW, WMGPY, and EMGPA, respectively). Moreover, WMFDW, WMGPY, and EMGPA showed strong capability in reducing power and lipd peroxidation inhibition in the linoleic acid system. In addition, WMFDW, WMGPY, and EMGPA can retain strong antioxidant activity at temperatures lower than 60 °C and pH values ranged from 5 to 9. The results showed that six isolated peptides (P1 to P6) from skipjack tuna heads, especially WMFDW, WMGPY, and EMGPA, might be applied in health care products acting as powerful antioxidant agents.

Observation on behaviours of Antarctic krill (Euphausia superba) in lighting condition

2012 ◽  
Vol 36 (2) ◽  
pp. 300
Author(s):  
Peng-xiang XU ◽  
Ying-chun LI ◽  
Guo-ping ZHU ◽  
Hui XIA ◽  
Liu-xiong XU
Keyword(s):  
Antarctic Krill ◽  
Euphausia Superba ◽  
Lighting Condition ◽  
Antarctic Krill Euphausia Superba

Observations on moulting in Antarctic Krill (Euphausia superba Dana)

1982 ◽  
Vol 33 (1) ◽  
pp. 71 ◽  
Author(s):  
T Ikeda ◽  
P Dixon
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Antarctic Krill ◽  
Dry Weight ◽  
Euphausia Superba ◽  
Dunaliella Tertiolecta ◽  
Antarctic Ocean ◽  
Fish Food ◽  
Antarctic Waters ◽  
The Antarctic ◽  
Antarctic Krill Euphausia Superba

Live E. superba were transported from Antarctic waters to a tropical laboratory where observations at the temperature of -0.5�C (0 to - 1.0�C), were made of intermoult period of specimens fed a mixture of microalgae (Dunaliella tertiolecta and Phaeodactylum tricornutum) or artificial pet fish food or starved. Mean intermoult period was 26.4-27.1 days for fed specimens and 29.6 days for starved specimens, with no relation to the size of specimens. The moult accounted for a loss of 2.63-4.35% of animal dry weight, which is equivalent to 1.1-1.8% of animal nitrogen or 1.4-2.3% of animal carbon. The contribution of moults to detritus in the Antarctic Ocean was estimated as 0.11 g C m-2 per year.

Altered developmental timing in early life stages of Antarctic krill (Euphausia superba) exposed to p,p′-DDE

2011 ◽  
Vol 409 (24) ◽  
pp. 5268-5276 ◽  
Author(s):  
Anita H. Poulsen ◽  
So Kawaguchi ◽  
Matti T. Leppänen ◽  
Jussi V.K. Kukkonen ◽  
Susan M. Bengtson Nash
Keyword(s):  
Early Life ◽  
Antarctic Krill ◽  
Euphausia Superba ◽  
Developmental Timing ◽  
Life Stages ◽  
Early Life Stages ◽  
Antarctic Krill Euphausia Superba

Ultrastructure of the Fertilized Egg of the Antarctic Krill Euphausia Superba

1985 ◽  
Vol 5 (3) ◽  
pp. 430-438 ◽  
Author(s):  
R. M. Dillaman ◽  
R. Y. George ◽  
J.-O. Strömberg
Keyword(s):  
Antarctic Krill ◽  
Euphausia Superba ◽  
The Antarctic ◽  
Fertilized Egg ◽  
Antarctic Krill Euphausia Superba
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