scholarly journals Isolation and Characterization of New Anti-Inflammatory and Antioxidant Components from Deep Marine-Derived Fungus Myrothecium sp. Bzo-l062

Marine Drugs ◽  
2020 ◽  
Vol 18 (12) ◽  
pp. 597
Author(s):  
Xiaojie Lu ◽  
Junjie He ◽  
Yanhua Wu ◽  
Na Du ◽  
Xiaofan Li ◽  
...  
Keyword(s):  
Deep Sea ◽  
High Performance ◽  
Oxygen Radical ◽  
Normal Phase ◽  
Oxide Formation ◽  
Nitric Oxide Formation ◽  
Isolation And Characterization ◽  
Anti Inflammatory ◽  
New Compounds

In the present study, four new compounds including a pair of 2-benzoyl tetrahydrofuran enantiomers, namely, (−)-1S-myrothecol (1a) and (+)-1R-myrothecol (1b), a methoxy-myrothecol racemate (2), and an azaphilone derivative, myrothin (3), were isolated along with four known compounds (4–7) from cultures of the deep-sea fungus Myrothecium sp. BZO-L062. Enantiomeric compounds 1a and 1b were separated through normal-phase chiral high-performance liquid chromatography. The absolute configurations of 1a, 1b, and 3 were assigned by ECD spectra. Among them, the new compound 1a and its enantiomer 1b exhibited anti-inflammatory activity, inhibited nitric oxide formation in lipopolysaccharide-treated RAW264.7 cells, and exhibited antioxidant activity in the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and oxygen radical absorbance capacity assays.

Preparation and Characterization of the High Molecular Weight [3H]Hyaluronic Acid

1992 ◽  
Vol 57 (10) ◽  
pp. 2151-2156 ◽  
Author(s):  
Peter Chabreček ◽  
Ladislav Šoltés ◽  
Hynek Hradec ◽  
Jiří Filip ◽  
Eduard Orviský
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
High Molecular Weight ◽  
Methyl Bromide ◽  
High Performance ◽  
Hydrogen Atoms ◽  
Isolation And Characterization ◽  
Labelling Procedure ◽  
Enzymatic Depolymerization

Two methods for the preparation of high molecular weight [3H]hyaluronic acid were investigated. In the first one, hydrogen atoms in the molecule were replaced by tritium. This isotopic substitution was performed in aqueous solution using Pd/CaCO3 as the catalyst. In the second method, the high molecular weight hyaluronic acid was alkylated with [3H]methyl bromide in liquid ammonia at a temperature of -33.5 °C. High-performance gel permeation chromatographic separation method was used for the isolation and characterization of the high molecular weight [3H]hyaluronic acid. Molecular weight parameters for the labelled biopolymers were Mw = 128 kDa, Mw/Mn = 1.88 (first method) and Mw = 268 kDa, Mw/Mn = 1.55 (second method). The high molecular weight [3H]hyaluronic acid having Mw = 268 kDa was degraded further by specific hyaluronidase. Products of the enzymatic depolymerization were observed to be identical for both, labelled and cold biopolymer. This finding indicates that the described labelling procedure using [3H]methyl bromide does not induce any major structural rearrangements in the molecule.

Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel

2011 ◽  
Vol 48 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Swagata Das ◽  
Biswajit Mishra ◽  
Kamaldeep Gill ◽  
Md. Saquib Ashraf ◽  
Abhay Kumar Singh ◽  
...  
Keyword(s):  
Aloe Vera ◽  
Isolation And Characterization ◽  
Anti Inflammatory ◽  
Anti Fungal ◽  
Novel Protein

scholarly journals Synthesis, Isolation and Characterization of Process-Related Impurities in Oseltamivir Phosphate

2012 ◽  
Vol 9 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Yogesh Kumar Sharma ◽  
Dau Dayal Agarwal ◽  
Sudesh Bhure ◽  
Sanjay Singh Rathore ◽  
Chakravir Rawat ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mass Number ◽  
Reverse Phase ◽  
Related Impurities ◽  
Isolation And Characterization ◽  
Bulk Drug ◽  
Oseltamivir Phosphate

Three known impurities in oseltamivir phosphate bulk drug at level 0.1% (ranging from 0.05-0.1%) were detected by gradient reverse phase high performance liquid chromatography. These impurities were preliminarily identified by the mass number of the impurities. Different experiments were conducted and finally the known impurities were synthesized and characterized.

Isolation and characterization of anti-inflammatory peptides derived from trypsin hydrolysis of microalgae protein (Synechococcus sp. VDW)

2019 ◽  
Vol 33 (4) ◽  
pp. 303-324 ◽  
Author(s):  
Rutairat Suttisuwan ◽  
Saranya Phunpruch ◽  
Tanatorn Saisavoey ◽  
Papassara Sangtanoo ◽  
Nuttha Thongchul ◽  
...  
Keyword(s):  
Isolation And Characterization ◽  
Trypsin Hydrolysis ◽  
Synechococcus Sp ◽  
Anti Inflammatory ◽  
Hydrolysis Of

scholarly journals Efficient resolution of racemic crown-shaped cyclotriveratrylene derivatives and isolation and characterization of the intermediate saddle isomer

2019 ◽  
Vol 15 ◽  
pp. 1339-1346
Author(s):  
Sven Götz ◽  
Andreas Schneider ◽  
Arne Lützen
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Nmr Spectroscopy ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Hplc Separation ◽  
Promising Alternative ◽  
Isolation And Characterization ◽  
C Nmr

The preparative resolution of a trifunctionalized C 3-symmetrical chiral cyclotriveratrylene derivative was achieved via high-performance liquid chromatography (HPLC) on a chiral stationary phase. This approach is a promising alternative to the previously reported resolution through formation of diastereomeric esters because it involves fewer synthetic steps and is less prone to thermal (re)racemization. During these studies an intermediate saddle conformer could also be isolated and characterized by 1H and 13C NMR spectroscopy. The HPLC separation method was further developed in order to allow investigations on the racemization behavior of the cyclotriveratrylene derivative.

scholarly journals Isolation and Characterization of Novel Psychrophilic, Neutrophilic, Fe-Oxidizing, Chemolithoautotrophic α- and γ-Proteobacteria from the Deep Sea

2003 ◽  
Vol 69 (5) ◽  
pp. 2906-2913 ◽  
Author(s):  
K. J. Edwards ◽  
D. R. Rogers ◽  
C. O. Wirsen ◽  
T. M. McCollom
Keyword(s):  
Deep Sea ◽  
Sequence Similarity ◽  
Heterotrophic Bacterium ◽  
Physiological Characterization ◽  
Metalliferous Sediments ◽  
Basalt Glass ◽  
Isolation And Characterization ◽  
Iron Oxidizing Bacteria ◽  
Microaerobic Conditions

ABSTRACT We report the isolation and physiological characterization of novel, psychrophilic, iron-oxidizing bacteria (FeOB) from low-temperature weathering habitats in the vicinity of the Juan de Fuca deep-sea hydrothermal area. The FeOB were cultured from the surfaces of weathered rock and metalliferous sediments. They are capable of growth on a variety of natural and synthetic solid rock and mineral substrates, such as pyrite (FeS2), basalt glass (∼10 wt% FeO), and siderite (FeCO3), as their sole energy source, as well as numerous aqueous Fe substrates. Growth temperature characteristics correspond to the in situ environmental conditions of sample origin; the FeOB grow optimally at 3 to 10°C and at generation times ranging from 57 to 74 h. They are obligate chemolithoautotrophs and grow optimally under microaerobic conditions in the presence of an oxygen gradient or anaerobically in the presence of nitrate. None of the strains are capable of using any organic or alternate inorganic substrates tested. The bacteria are phylogenetically diverse and have no close Fe-oxidizing or autotrophic relatives represented in pure culture. One group of isolates are γ-Proteobacteria most closely related to the heterotrophic bacterium Marinobacter aquaeolei (87 to 94% sequence similarity). A second group of isolates are α-Proteobacteria most closely related to the deep-sea heterotrophic bacterium Hyphomonas jannaschiana (81 to 89% sequence similarity). This study provides further evidence for the evolutionarily widespread capacity for Fe oxidation among bacteria and suggests that FeOB may play an unrecognized geomicrobiological role in rock weathering in the deep sea.

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