scholarly journals Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion

Marine Drugs ◽  
2020 ◽  
Vol 18 (7) ◽  
pp. 353
Author(s):  
Luz Verónica Pacheco ◽  
Javier Parada ◽  
José Ricardo Pérez-Correa ◽  
María Salomé Mariotti-Celis ◽  
Fernanda Erpel ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Water Extract ◽  
Starch Digestion ◽  
Total Polyphenols ◽  
Functional Ingredients ◽  
Durvillaea Antarctica ◽  
Common Illnesses ◽  
Starchy Foods ◽  
Environmentally Friendly Method

The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the Durvillaea antarctica (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity.

scholarly journals EFFECT OF EXTRACTION CONDITIONS ON POLYPHENOLS, FLAVONOIDS, S-ALLYL CYSTEINE CONTENT AND ANTIOXIDANT ACTIVITY OF BLACK GARLIC EXTRACTS

2018 ◽  
Vol 55 (5A) ◽  
pp. 18
Author(s):  
Nguyen Ai Thach
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Suitable Condition ◽  
Solvent Ratio ◽  
Scavenging Activity ◽  
Total Polyphenols ◽  
Beneficial Effects ◽  
Cysteine Content

Black garlic is obtained from fresh garlic (Allium sativum L.) that has been aged for a long time at a high temperature under high humidity. Black garlic exerts metabolic and cardiovascular beneficial effects. In this study, (i) water and various concentrations of ethanol (50 % and 100 %) in water were used as solvent in the extraction of black garlic with different black garlic:solvent ratios (1:5, 1:10 and 1:15 w/v); (ii) the process was investigated in the temperatures ranging of 40 to 80 oC during 30 to 120 minutes, as well as determination of bioactive compounds content (total polyphenols, total flavonoids and S-allyl cysteine content) of all obtained extracts. The antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The obtained results indicated that the aqueous ethanol (50 %) extracts presented the highest contents of polyphenols and flavonoids (7.94 mg GAE/g and 3.37 mg QE/g, respectively) and DPPH radical scavenging activity at black garlic: solvent ratio of 1:10 (w/v); while the water extract showed the highest S-allyl cysteine content (76.0 mg/kg) at the 1:10 ratio. The suitable condition for extracting of total polyphenols and flavonoids from black garlic was 60 oC for 90 minutes. With respect to SAC extraction, the suitable temperature and time of 80 oC and 90-120 minutes, respectively  were found.   

STYDY OF THE INFLUENCE OF FLAKING EINKORN, ON FOOD VALUE, IN VITRO ANTIOXIDANT ACTIVITY AND STARCH DIGESTION CONTEND ON BISCUITS

2018 ◽  
pp. 254-265
Author(s):  
Ivan Dimov ◽  
Gjore Nakov ◽  
Viktorija Stamatovska
Keyword(s):  
Antioxidant Activity ◽  
Starch Digestion ◽  
Total Polyphenols ◽  
Total Carotenoids ◽  
Active Compounds ◽  
Total Carbohydrates ◽  
Food Value ◽  
In Vitro Antioxidant Activity

The influence of flaked einkorn (Tiriticum monococcum L.) used as ingredient in production of biscuits on some of their chemical, biological active compounds and In vitro starch digestion. The biscuits were produced from whole einkorn flour to which 30 %, 50 %, 70 % and 100 % einkorn flakes were added at the expense of the einkorn flour. It was found from the results with increase flaking einkorn in biscuits increase: moisture, total fats and proteins contend in the biscuits and decreases ash and total carbohydrates. Form the results for biological active compounds with increase flaking einkorn in biscuits, decreases total polyphenols and increase total carotenoids and antioxidant activity. In vitro starch digestion showed that when biscuits contend more then 30 % of einkorn flour reduces the body's ability to break down the starch contained in biscuits.

Contribution of Major Polyphenols to the Antioxidant Profile and Cytotoxic Activity of Olive Leaves

2019 ◽  
Vol 19 (13) ◽  
pp. 1651-1657
Author(s):  
Zouhaier Bouallagui ◽  
Asma Mahmoudi ◽  
Amina Maalej ◽  
Fatma Hadrich ◽  
Hiroko Isoda ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Cytotoxic Activities ◽  
Phenolic Composition ◽  
Olive Leaves ◽  
Total Polyphenols ◽  
Cytotoxic Effects ◽  
Antioxidant Profile ◽  
Phytochemical Profile ◽  
Mcf 7

Aim: This study was designed to investigate the phytochemical profile and the cytotoxic activities of the eco-friendly extracts of olive leaves from Chemlali cultivar. Materials and Methods: The Phenolic composition of olive leaves extracts, the antioxidant activity and the cytotoxic effects against MCF-7 and HepG2 cells were determined. Results: Olive leaves extracts showed relevant total polyphenols contents. Oleuropein was the major detected phenolic compound reaching a concentration of 16.9 mg/ml. The antioxidant potential of the studied extracts varied from 23.7 to 46.5mM Trolox equivalents as revealed by DPPH and ABTS assays. Cytotoxicity experiments showed similar trends for both HepG2 and MCF-7 cells with the infusion extract being the most active. Conclusion: This study denotes that olive leaves may have great potential as endless bioresource of valuable bioactive compounds which may have a wide application.

scholarly journals Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

2021 ◽  
Vol 11 (8) ◽  
pp. 3634
Author(s):  
Teresa Leszczyńska ◽  
Barbara Piekło ◽  
Aneta Kopeć ◽  
Benno F. Zimmermann
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Vitamin C ◽  
Dietary Fiber ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Total Polyphenols ◽  
Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

scholarly journals Effect of Ultrasound-Assisted Extraction Parameters on Total Polyphenols and Its Antioxidant Activity from Mango Residues (Mangifera indica L. var. Manililla)

Separations ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 94
Author(s):  
Anahí J. Borrás-Enríquez ◽  
Elizabeth Reyes-Ventura ◽  
Socorro J. Villanueva-Rodríguez ◽  
Lorena Moreno-Vilet
Keyword(s):  
Antioxidant Activity ◽  
Mangifera Indica ◽  
Central Point ◽  
Added Value ◽  
Sonication Time ◽  
Ultrasound Assisted Extraction ◽  
Extraction Technology ◽  
Total Polyphenols ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Manililla is a mango variety whose residues contain bioactive compounds such as polyphenols and flavonoids, with high added value. The use of environmentally friendly extraction technology would be of great relevance; hence, this study aimed to evaluate the effect of solvent relation, sonication time and amplitude on the ultrasound-assisted extraction of total polyphenols in Manililla mango residues (peel, endocarp and kernel) and antioxidant activity. An experimental design 23 with a central point was used to evaluate the curvature behavior of the process variables. Conventional maceration was used as a control. The better conditions were obtained at the central point using 50% ethanol in water, 60% amplitude and 20 min of sonication time. We obtained values of up to 1814 mg GAE/100 g, 469 mg GAE/100 g and 672 mg GAE/100 g of total polyphenols and 1228 mg QE/100 g, 653 mg QE/100 g and 880 mg QE/100 g of total flavonoids for peel, endocarp and kernel, respectively. Mangiferin was quantified in ultrasound-assisted extraction at 150 mg/g in peel and 0.025 mg/g in the kernel, but it was not detectable in maceration. An antioxidant capacity of 87%, 14% and 83% inhibition for peel, endocarp and kernel, respectively, were obtained. Peel and kernel were the residues with higher potential as extraction material, while endocarp was not.

scholarly journals In Vitro Antioxidant Activity of Litsea martabanica Root Extract and Its Hepatoprotective Effect on Chlorpyrifos-Induced Toxicity in Rats

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1906
Author(s):  
Phraepakaporn Kunnaja ◽  
Sunee Chansakaow ◽  
Absorn Wittayapraparat ◽  
Pedcharada Yusuk ◽  
Seewaboon Sireeratawong
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Acetylcholinesterase Activity ◽  
Liver Cells ◽  
Scientific Data ◽  
Total Phenolic ◽  
Root Extract ◽  
Antioxidant Power ◽  
Detoxifying Agent

In Thailand, people in the highland communities whose occupational exposure to pesticides used the root of Litsea martabanica as a detoxifying agent. However, the scientific data to support the traditional use of this plant are insufficient. This study aimed to evaluate the antioxidant activity and anti-pesticide potential of L. martabanica root extract. Antioxidant properties were investigated by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay, superoxide radicals scavenging assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing antioxidant power (FRAP), and total phenolic content determination. In all assays, L. martabanica extracts and their fractions exhibited high antioxidant activities differently. The water extract is traditionally used as a detoxifying agent. Therefore, it was chosen for in vivo experiments. The rats received the extract in a way that mimics the traditional methods of tribal communities followed by chlorpyrifos for 16 days. The results showed that acetylcholinesterase activity decreases in pesticide-exposed rats. Treatment with the extract caused increasing acetylcholinesterase activity in the rats. Therefore, L. martabanica extract may potentially be used as a detoxifying agent, especially for the chlorpyrifos pesticide. The antioxidant properties of L. martabanica may provide a beneficial effect by protecting liver cells from damage caused by free radicals. Histopathology results revealed no liver cell necrosis and showed the regeneration of liver cells in the treatment group. L. martabanica extract did not cause changes in behavior, liver weight, hematological and biochemical profiles of the rats.

Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth

2009 ◽  
Vol 115 (3) ◽  
pp. 994-998 ◽  
Author(s):  
Paweł Paśko ◽  
Henryk Bartoń ◽  
Paweł Zagrodzki ◽  
Shela Gorinstein ◽  
Maria Fołta ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Polyphenols
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