scholarly journals The Nutritional and Pharmacological Potential of New Australian Thraustochytrids Isolated from Mangrove Sediments

Marine Drugs ◽  
2020 ◽  
Vol 18 (3) ◽  
pp. 151 ◽  
Author(s):  
Thi Linh Nham Tran ◽  
Ana F. Miranda ◽  
Adarsha Gupta ◽  
Munish Puri ◽  
Andrew S. Ball ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Extracellular Polysaccharide ◽  
Buffer Zone ◽  
Added Value ◽  
Oil Bodies ◽  
High Concentration ◽  
Mangrove Sediments ◽  
Aqueous Emulsion ◽  
Total Fatty Acids

Mangrove sediments represent unique microbial ecosystems that act as a buffer zone, biogeochemically recycling marine waste into nutrient-rich depositions for marine and terrestrial species. Marine unicellular protists, thraustochytrids, colonizing mangrove sediments have received attention due to their ability to produce large amounts of long-chain ω3-polyunsaturated fatty acids. This paper represents a comprehensive study of two new thraustochytrids for their production of valuable biomolecules in biomass, de-oiled cakes, supernatants, extracellular polysaccharide matrixes, and recovered oil bodies. Extracted lipids (up to 40% of DW) rich in polyunsaturated fatty acids (up to 80% of total fatty acids) were mainly represented by docosahexaenoic acid (75% of polyunsaturated fatty acids). Cells also showed accumulation of squalene (up to 13 mg/g DW) and carotenoids (up to 72 µg/g DW represented by astaxanthin, canthaxanthin, echinenone, and β-carotene). Both strains showed a high concentration of protein in biomass (29% DW) and supernatants (2.7 g/L) as part of extracellular polysaccharide matrixes. Alkalinization of collected biomass represents a new and easy way to recover lipid-rich oil bodies in the form of an aqueous emulsion. The ability to produce added-value molecules makes thraustochytrids an important alternative to microalgae and plants dominating in the food, pharmacological, nutraceutical, and cosmetics industries.

Long-chain n-3 polyunsaturated fatty acids contained in liver and muscle tissues of Okinawan long-spine porcupinefish (Diodon holocanthus Linnaeus 1758)

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Yutaka Tashiro
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Ryukyu Islands ◽  
Okinawa Island ◽  
Content Type ◽  
Muscle Tissues ◽  
The Ryukyu Islands ◽  
Total Fatty Acids ◽  
Long Chain

Purpose This study aimed to analyze the lipid content and fatty acid composition in the liver and muscle of a porcupinefish species inhabiting waters around the Ryukyu Islands to investigate their potential as a source of long-chain n-3 polyunsaturated fatty acids (LC-PUFAs). Design/methodology/approach Porcupinefish were collected along the Okinawa Island coast. The composition of fatty acids and cholesterol in both liver and muscle were analyzed using a gas chromatograph mass spectrometer. Findings The liver of Okinawan long-spine porcupinefish was rich in lipids whose content correlated to the proportion of liver/body weight. Fatty acid compositions in their liver and muscles were similar to each other. LC-PUFAs occupied 44% of total fatty acids, with docosahexaenoic acid (DHA) being the dominant (42%), whereas eicosapentaenoic acid occupied 2.4%. The liver contained 1,690 mg of cholesterol and 14.8 g of DHA per 100 g, whose proportion decreased in summer compared to other seasons (p = 0.036). Originality/value The liver of Okinawan long-spine porcupinefish, which has not yet been commercially used although its non-toxicity is claimed, can be an excellent source of LC-PUFAs, especially DHA, accentuating its potential in food supplements’ production.

Lipids: Fatty Acids

2011 ◽  
Author(s):  
Thomas S. Bianchi ◽  
Elizabeth A. Canuel
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Deep Sea ◽  
Saturated Fatty Acids ◽  
Relative Proportion ◽  
Aquatic Organisms ◽  
Building Blocks ◽  
Deep Sea Bacteria ◽  
Total Fatty Acids ◽  
Fatty Acid Biomarkers

This chapter discusses fatty acids, the building blocks of lipids, which represent a significant fraction of the total lipid pool in aquatic organisms. It explores how chain length and levels of unsaturation (number of double bonds) have been shown to be correlated to decomposition, indicating a pre- and postdepositional selective loss of short-chain and polyunsaturated fatty acids. In contrast, saturated fatty acids are more stable and typically increase in relative proportion to total fatty acids with increasing sediment depth. Polyunsaturated fatty acids (PUFAs) are predominantly used as proxies for the presence of “fresh” algal sources, although some PUFAs also occur in vascular plants and deep-sea bacteria. Thus, these biomarkers represent a very diverse group of compounds present in aquatic systems. The numerous applications of fatty acid biomarkers to identifying the sources of organic matter in lakes, rivers, estuaries, and marine ecosystems are discussed.

scholarly journals Improvement of Wax Oil Content of Embryonic Callus of Jojoba Using Gamma Radiation

2019 ◽  
Vol 29 (2) ◽  
pp. 207-217
Author(s):  
Hattem M. El-Shabrawi ◽  
Hend E. Wahba ◽  
Ahmed M. Gabr ◽  
Shafik I. El-Morsy ◽  
Mohamed A. Saber ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gamma Radiation ◽  
Embryogenic Callus ◽  
Oil Content ◽  
Leaf Explants ◽  
Simmondsia Chinensis ◽  
Oil Bodies ◽  
Embryonic Callus ◽  
Total Fatty Acids ◽  
Different Doses

Embryogenic callus was obtained from the leaf explants of jojoba (Simmondsia chinensis (Link) Schneider cultured on MS containing 0.5 mg/l NAA and 0.5 mg/l Kn. Growth of embryonic callus increased in 0.5 mg/l Kn + 6% sucrose. In order to improve oil content, the embryonic callus was exposed to different doses (5, 10 and 15 Kr) of gamma radiation. It was found that oil content of embryonic callus of jojoba increased 1.41% by exposing to 5 Kr gamma radiation. Also, size of oil bodies in embryonic callus irradiated with 5 Kr increased compared to control. Furthermore, production of the fatty acids was enhanced by gamma irradiation. The highest percentage of total fixed oil (1.41) was recorded in 5 Kr radiation. However, the highest content of total fatty acids (98.612) was obtained when embryonic callus were exposed to 10 Kr of gamma radiation.

scholarly journals Effect of feeding tuna oil or soyabean oil as supplements to sows in late pregnancy on piglet tissue composition and viability

1998 ◽  
Vol 80 (3) ◽  
pp. 273-280 ◽  
Author(s):  
J. A. Rooke ◽  
I. M. Bland ◽  
S. A. Edwards
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Late Pregnancy ◽  
Tissue Composition ◽  
Tuna Oil ◽  
Total Fatty Acids ◽  
Effect Of Feeding ◽  
Long Chain ◽  
Viability Score

To investigate whether long-chain n-3 polyunsaturated fatty acids could cross the porcine placenta in late pregnancy and alter neonatal piglet tissue composition, multiparous sows (seven per diet) were fed on diets containing a supplement (30 g/kg) of either soyabean oil or tuna oil for the last 21 d of pregnancy and the first 7 d of lactation. The proportions of all fatty acids, except 18:1n-7, differed between diets: in particular, the tuna-oil-containing diet supplied more 22:6n-3 and less 18:2n-6 fatty acids than the soyabean-oil-containing diet. The proportions of n-3 fatty acids, particularly 22:6n-3 (g/100 g total fatty acids) in sow plasma, colostrum and milk were increased and the proportion of 18:2n-6 was decreased by feeding tuna oil. Piglet tissue n-3 fatty acid proportions (particularly 22:6n-3), obtained shortly after birth, were increased in piglets born to tuna-oil-fed sows compared with progeny of soyabean-oil-fed sows. The increase in the proportion of n-3 fatty acids (g/100 g total fatty acids) in piglet tissues as a result of tuna-oil feeding, compared with soyabean-oil-feeding, was in the order plasma>liver>erythrocytes>spleen>brain>retina. Piglets born to tuna-oil-fed sows had a lower viability score at birth than the progeny of soyabean-oil-fed sows. The proportions of long-chain n-3 fatty acid in tissues of new-born piglets were increased by feeding tuna oil to the sow in late pregnancy; however no improvements in piglet viability were observed.

scholarly journals Oil and Fatty Acid Composition of Tepary Bean Seed

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1436-1438 ◽  
Author(s):  
Harbans L. Bhardwaj ◽  
Anwar A. Hamama
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Bean Seed ◽  
High Concentration ◽  
Tepary Bean ◽  
Planting Dates ◽  
Promising Alternative ◽  
Food And Feed ◽  
Oil Concentration ◽  
Pinto Beans

Information about oil and fatty acids in tepary bean (Phaseolus acutifolius A. Gray) seed, a promising alternative crop for the mid-Atlantic region of U.S., is largely unknown. Such information is needed to assess the food and feed potentials of tepary bean seed. We determined the concentrations of oil and fatty acids in seed produced by eight tepary bean genotypes planted at three different dates each during 1997 and 1998 at Ettrick, Va. Tepary bean seeds contained 1.8% oil as compared to literature values of 1.3%, 1.1%, and 1.1% for navy, kidney, and pinto beans, respectively. Tepary bean seed oil contained 33% saturated, 67% unsaturated, 24% monounsaturated, and 42% polyunsaturated fatty acids. Planting dates and genotypes did not affect oil concentration. Neb-T-14 was identified to be a desirable genotype based on a low concentration of saturated and a high concentration of polyunsaturated fatty acids. Based on concentrations of oil and fatty acids, tepary bean seeds compared well with those of navy, kidney, and pinto beans.

Badanie stabilności oleju lnianego i oleju z czarnuszki

2018 ◽  
Vol 19 (3) ◽  
Author(s):  
Agata Pawłowska ◽  
Arkadiusz Kocur ◽  
Paweł Siudem ◽  
Katarzyna Paradowska
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Linseed Oil ◽  
Acid Value ◽  
Storage Conditions ◽  
Antioxidant Compounds ◽  
High Concentration ◽  
Black Cumin ◽  
Daily Diet ◽  
The Impact

Introduction. Because of high concentration of monounsaturated and polyunsaturated fatty acids plant oils are increasingly popular ingredient of daily diet. Polyunsaturated fatty acids, which are not synthetized in the human body, are particularly important, because they are necessary for regular functioning of nervous and cardiovascular systems. Linseed oil and black cumin oil could be the source of these acids, but while consuming them it is worth knowing how conditions and time of storage influence their properties. Aim. Examination of changes of properties of linseed oil and black cumin oil depending on storage conditions. Material and methods. For both oils were four types of samples: collected right after opening, stored for a month in refrigerator, stored in 40°C and heated in microwave to 137°C. For each type of sample acid value, peroxide number were determined by titration, optical density (in 232 and 270 nm) and DPPH inhibition were measured spectrophotometrically. Results. Based on a results of analysis of changes in linseed oil an black cumin oil we observed the impact of high temperature on promotion of oxidation processes in linseed oil, while the black cumin oil was stable. Conclusions. Linseed oil requires low temperature of storage and can not be used after heating. Black cumin oil is more stable because of antioxidant compounds contained in essential oil.

scholarly journals Feasibility Study for Production of Biofuel and Chemicals from Marine Microalgae Nannochloropsis sp. Based on Basic Mass and Energy Analysis

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Simon Jegan Porphy ◽  
Mohammed M. Farid
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Content ◽  
Lipid Extraction ◽  
Biodiesel Production ◽  
High Concentration ◽  
Algae Biomass ◽  
Waste Land ◽  
Bio Oil ◽  
Total Fatty Acids ◽  
Solid Liquid

Algae are believed to be a good source of renewable energy because of their rapid growth rate and their ability to be cultivated in waste waters or waste land. The algae Nannochloropsis sp. was chosen for this study, where lipids were extracted and transesterified for biodiesel production. The FFA (free fatty acid) content in the lipid was estimated to be 27 wt% of the total fatty acids. The remaining biomass after lipid extraction was pyrolyzed at 200°C, 300°C, and 400°C to produce solid, liquid, and gas products. The GC/MS showed that the lipids of Nannochloropsis sp. consist of high concentration of polyunsaturated fatty acids (29 wt%), eicosapentaenoic acid. The bio-oil produced from pyrolysis of algae biomass (after lipid extraction) at 300°C was composed of 50 wt% acetone, 30 wt% methyl ethyl ketone and 19 wt% aromatics such as pyrazine and pyrrole. The heating value of bio-oil is 32 MJ/kg of oil.

Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids

2001 ◽  
Vol 73 (2) ◽  
pp. 253-260 ◽  
Author(s):  
K. Raes ◽  
S. de Smet ◽  
D. Demeyer
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Total Fatty Acid Content ◽  
Total Fatty Acids

AbstractThe effect of double-muscling (DM) genotype (double-muscling, mh/mh; heterozygous, mh/+; normal, +/+) of Belgian Blue (BB) young bulls on the intramuscular fatty acid composition, in particular conjugated linoleic acid (CLA) and polyunsaturated fatty acids (PUFA) was examined in five different muscles. The relative fatty acid composition showed only minor differences between muscles within genotypes. However, the DM genotype had a large effect on both the intramuscular total fatty acid content and on the relative fatty acid composition. Across muscles, the mh/mh animals had a lower total fatty acid content compared with the +/+animals (907 v: 2656 mg/100 g muscle;P< 0·01) and a higher PUFA proportion in total fatty acids (27·5 v 11·3 g/100 g total fatty acids;P< 0001), resulting in a higher PUFA/saturated fatty acid ratio (0·55 v 0·18;P< 0·01) and a lower n-6/n-3 ratio (5·34 v. 6·17;P< 0·01). The heterozygous genotype was intermediate between the two homozygous genotypes. The relative CLA content was similar in the mh/mh and +/+ genotypes and approximated 0·4 to 0·5 g/100 g total fatty acids. From the data it is further suggested that differences in the metabolism of the n-3 and n-6 fatty acids could exist between DM genotypes.

scholarly journals Neuroinflammation in Autism and Supplementation Based on Omega-3 Polyunsaturated Fatty Acids: A Narrative Review

Medicina ◽  
2021 ◽  
Vol 57 (9) ◽  
pp. 893
Author(s):  
Aleksandra Veselinović ◽  
Snježana Petrović ◽  
Vladica Žikić ◽  
Miško Subotić ◽  
Vladimir Jakovljević ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Neurodevelopmental Disorder ◽  
Autism Spectrum ◽  
Added Value ◽  
Omega 3 ◽  
Behavioral Deficits ◽  
Pufa Supplementation ◽  
Children With Asd ◽  
Patterns Of Behavior

Autism Spectrum Disorder (ASD) is a complex neurodevelopmental disorder characterized by persistent deficits in social communication and social interaction across multiple contexts and restricted, repetitive patterns of behavior, interests and activities. The maternal status of polyunsaturated fatty acids (PUFA) regulates microglial activity and neuroinflammatory pathways during a child’s brain development. In children with ASD, the metabolism of PUFA is thought to be deficient or abnormal, leading to increased production of proinflammatory cytokines, increased oxidative stress and an imbalance in the formation and action of neurotransmitters. In addition, nutritional deficits in omega-3 PUFA may affect gut microbiota and contribute to ASD by the gut–brain axis. The aim of this study was to review the possible role of neuroinflammation in ASD development and the effect of omega-3 PUFA supplementation in children with ASD. Due to a wide heterogeneity across RCTs, no definitive conclusion about omega-3 PUFA effects in ASD can be drawn. Supplementation with PUFA could be considered as one of the aspects in regulating the biological status of the organism and could provide added value to standard medical and psychological interventions for reducing behavioral deficits.

Biohydrogenation of n-3 polyunsaturated fatty acids in the rumen and their effects on microbial metabolism and plasma fatty acid concentrations in sheep

2005 ◽  
Vol 81 (2) ◽  
pp. 239-248 ◽  
Author(s):  
L. A. Sinclair ◽  
S. L. Cooper ◽  
S. Chikunya ◽  
R. G. Wilkinson ◽  
K. G. Hallett ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Formic Acid ◽  
Fish Oil ◽  
Marine Algae ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Microbial Efficiency ◽  
Total Fatty Acids

AbstractSix cannulated wether sheep weighing 57 (s.d. 4·3) kg were used to investigate the susceptibility of unprotected and protected n-3 polyunsaturated fatty acids from different sources to biohydrogenation in the rumen, their uptake into plasma and effects on ruminal metabolism. The sheep were assigned to one of six dietary treatments formulated to have a similar fatty acid content (60 g/kg DM) and containing: linseed oil (LO), linseed oil absorbed into vermiculite (VLO), formic acid-formaldehyde treated whole linseed (FLS), fish oil (FO), fat encapsulated fish oil (PFO) or a mixture of fish oil and marine algae (1: 1 on an oil basis; AF), in six periods of 28 days duration in a Latin-square design. Biohydrogenation of C20:5(n-3) and C22:6(n-3) was high in FO at approximately 870 g/kg, but reduced to 625 and 625 g/kg respectively for PFO, and 769 and 601 g/kg respectively for AF. Ruminal biohydrogenation of C18:3(n-3) was similar across treatments based on linseed, averaging 860 g/kg, but C18:2(n-6) was lower (P< 0·05) in animals given VLO or FLS at 792 and 837 g/kg respectively, compared with LO (907 g/kg). Duodenal flow of C18:1trans in animals given any of the diets containing fish oil averaged 8·4 g/day compared with 2·8 g/day in animals given diets based on linseed (P< 0·001), whilst cis-9, trans-11 conjugated linoleic acid was not significantly different among treatments. Plasma C20:5(n-3) and C22:6(n-3) proportions were highest in animals given the AF diet (11·8 and 8·2 g per 100 g of the total fatty acids respectively) and lowest in animals given LO (2·8 and 2·7 g per 100 g of the total fatty acids respectively;P< 0·001). By contrast, plasma C18:3(n-3) proportions were highest in animals given the LO or VLO diets at approximately 6·9 g per 100 g of the total fatty acids, and lowest in the AF treatment at 0·9 g per 100 g (P< 0·001). Duodenal non-ammonia-N flow was similar among treatments at 21·0 g/day except in animals given FLS which had the highest flow (25·9 g N per day;P< 0·01). Microbial N flow was also similar among treatments whilst microbial efficiency (g N per kg OM truly degraded in the rumen) was higher (P< 0·05) in animals given FLS than LO, FO or AF. By contrast, ruminal fibre digestion was higher (P< 0·05) in animals given LO or FO than those offered VLO, FLS, PFO or AF. In conclusion, compared with linseed oil, absorption of linseed oil into vermiculite improved duodenal flow but not plasma levels of C18:3(n-3), whilst formic acid-formaldehyde treatment of linseed had little effect on protecting C18:3(n-3) in the rumen, although duodenal non-ammonia nitrogen flow and microbial efficiency were improved. Compared with fish oil, the provision of marine algae or fat encapsulated fish oil resulted in a lower biohydrogenation of C22:6(n-3) and C20:5(n-3), and an increased duodenal flow and plasma concentration and offers the potential to favourably manipulate the n-3 fatty acid composition of sheep meat.

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