scholarly journals Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae

Marine Drugs ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 122 ◽  
Author(s):  
Noémie Coulombier ◽  
Elodie Nicolau ◽  
Loïc Le Déan ◽  
Cyril Antheaume ◽  
Thierry Jauffrais ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Light Intensity ◽  
Antioxidant Capacity ◽  
Peroxyl Radical ◽  
New Caledonia ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Pigment Analysis ◽  
Tbars Assay

Twelve microalgae species isolated in tropical lagoons of New Caledonia were screened as a new source of antioxidants. Microalgae were cultivated at two light intensities to investigate their influence on antioxidant capacity. To assess antioxidant property of microalgae extracts, four assays with different modes of action were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulphonique) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobabituric acid reactive substances (TBARS). This screening was coupled to pigment analysis to link antioxidant activity and carotenoid content. The results showed that none of the microalgae studied can scavenge DPPH and ABTS radicals, but Chaetoceros sp., Nephroselmis sp., and Nitzschia A sp. have the capacity to scavenge peroxyl radical (ORAC) and Tetraselmis sp., Nitzschia A sp., and Nephroselmis sp. can inhibit lipid peroxidation (TBARS). Carotenoid composition is typical of the studied microalgae and highlight the siphonaxanthin, detected in Nephroselmis sp., as a pigment of interest. It was found that xanthophylls were the major contributors to the peroxyl radical scavenging capacity measured with ORAC assay, but there was no link between carotenoids and inhibition of lipid peroxidation measured with TBARS assay. In addition, the results showed that light intensity has a strong influence on antioxidant capacity of microalgae: Overall, antioxidant activities measured with ORAC assay are better in high light intensity whereas antioxidant activities measured with TBARS assay are better in low light intensity. It suggests that different antioxidant compounds production is related to light intensity.

scholarly journals Anti-urease, total phenolic content and antioxidant activities of some Inula l. (Asteraceae) taxa in Turkey

Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 825-834
Author(s):  
Emre Sevindik ◽  
Sinem Aydin ◽  
Mehmet Paksoy ◽  
Bahar Sokmen
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Soxhlet Extraction ◽  
Total Phenolic ◽  
Food Industries ◽  
Total Antioxidant ◽  
Ethanol Extracts

The genus Inula belongs to family Asteraceae and it is medicinally important because it possesses cytotoxic, anticancer, hepatoprotective, antibacterial and anti-inflammatory properties. The aim of this research was to explore antioxidant and anti-urease activities of fifteen Inula taxa in Turkey. Ethanol extracts of Inula taxa were obtained by Soxhlet extraction. Antioxidant activity was determined through total antioxidant activity, total phenolic content and copper reducing antioxidant capacity (CUPRAC) analyses. The highest total phenolic content and total antioxidant capacity were found in I. orientalis (158.54?0.002 ?g GAE/mL) and I. macrocephala (189.16?0.002 ?g AAE/mL). CUPRAC activity increases with the increasing concentration of the extracts and the standard. Moreover, all the tested plants exhibited better anti-urease activity than standard thiourea except for I. anatolica and I. thapsoides subsp. thapsoides. Results obtained from this study demonstrate that Inula species might be utilized as a probable natural source in the pharmaceutical and food industries but further and more specific studies are needed to determine active antioxidative and anti-urease compounds in the extracts.

Studies on the Antioxidant Activities of Total Alkaloids from the Fruits of Evodia rutaecarpa (Juss.) Benth

2011 ◽  
Vol 396-398 ◽  
pp. 52-55 ◽  
Author(s):  
Ming Xiong Tan ◽  
Yan Cheng Liu ◽  
Xu Jian Luo ◽  
Dong Qing Li
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Dpph Radical ◽  
Synthetic Antioxidant ◽  
Total Alkaloids ◽  
Evodia Rutaecarpa ◽  
Total Antioxidant

The total alkaloids (TAE) were obtained from the fruits of Evodia rutaecarpa (Juss.) Benth. The antioxidant activities of TAE towards the inhibitory effect on 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) free radical, total antioxidant capacity, and inhibition on lipid peroxidation were investigated. TAE was found that the total antioxidant capacity and inhibitory lipid peroxidation are superior to synthetic antioxidant 2, 6-di-ter-butyl-4-methylphenol (BHT), but scavenging activity on DPPH radical is lower than that of BHT at the same condition.

scholarly journals Comparative Study of the Antioxidant Activities of Eleven Salvia Species

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Gábor Janicsák ◽  
István Zupkó ◽  
Imre Máthé ◽  
Judit Hohmann
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biological System ◽  
Antioxidant Activities ◽  
Close Correlation ◽  
Reference Compound ◽  
Total Polyphenol ◽  
Methanolic Extracts

The lipid peroxidation-inhibiting activities of aqueous methanolic extracts of eleven Salvia species (Fam. Lamiaceae) were evaluated in an enzyme-independent biological system. The total polyphenol contents and the amounts of the most abundant phenoloids of the genus, caffeic and rosmarinic acids, were also determined. The EC50 values of the extracts displayed substantial differences. All of the investigated species except S. jurisicii (EC50 191.2 μg/mL) exhibited higher activities than that of ascorbic acid (EC50 123.8 μg/mL), the reference compound. Among the studied species, S. scabiosifolia (EC50 5.4 μg/mL) demonstrated the highest effect, followed in sequence by S. dumetorum, S. transsylvanica, S. officinalis l albiflora, S. nemorosa l albiflora and S. recognita (EC50 6.5 – 10.2 μg/mL). The close correlation was confirmed between the antioxidant activities and the total phenol contents of the extracts. For caffeic and rosmarinic acids, the correlation was much weaker, indicating the important role of other polyphenols in the antioxidant activity.

A Study on the Antioxidant Activity and Tissues Selective Inhibition of Lipid Peroxidation by Saponins from the Roots of Platycodon grandiflorum

2009 ◽  
Vol 37 (05) ◽  
pp. 967-975 ◽  
Author(s):  
Xian-Jun Fu ◽  
Hong-Bing Liu ◽  
Peng Wang ◽  
Hua-Shi Guan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Selective Inhibition ◽  
Antioxidant Systems ◽  
Platycodon Grandiflorum ◽  
Platycodi Radix ◽  
Antioxidant Effects ◽  
Different Tissues ◽  
Selective Effects

Platycodi Radix is the root of Platycodon grandiflorum (Jacq.) A. DC and has been used as a traditional medicine in China. According to the theory of traditional Chinese medicine, Platycodi Radix (PR) possesses the character of lung meridian tropism (Guijing) and has selective effects on the lung and respiratory system. The aim of this study was to confirm the antioxidant effects of saponins from Platycodi Radix (PRS), with emphasis on its selective inhibition of lipid peroxidation in different tissues. The hydroxyl radical scavenging activity was determined by a H2O2/Fe2+ system; a modified thiobarbituric acid reactive species assay was used to measure the lipid peroxide in rats' tissues. These antioxidant activities were compared to ascorbic acid (Vc). The results showed that PRS had antioxidant activities in various antioxidant systems. And the inhibition capability of lipid peroxidation of PRS and Vc were excellent, but differed greatly in different tissues. These results suggested that PRS had antioxidant effects and selective inhibition of lipid peroxidation. It indicated that the mechanism of Platycodi Radix to treat some diseases might be related to its antioxidant activity, especially its tissue selective effects. However, further study is needed.

scholarly journals Evaluation of Antioxidant Capacity ofSolanum sessiliflorum(Cubiu) Extract: AnIn VitroAssay

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Diego Rocha de Lucena Herrera Mascato ◽  
Janice B. Monteiro ◽  
Michele M. Passarinho ◽  
Denise Morais Lopes Galeno ◽  
Rubén J. Cruz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Flavonoid Glycosides ◽  
Specific Staining ◽  
Native Populations ◽  
Chemical Groups ◽  
Solanaceae Family ◽  
Hydroethanolic Extract

Cubiu is a vegetable of Solanaceae family, native to the Amazon, which is widely distributed through Brazil, Peru, and Colombia. It is used in food, medicine, and cosmetics by native populations. Research has shown that cubiu extracts have antioxidant activities with great biological relevance. We performed a phytochemical screening to identify the main chemical groups that could confer antioxidant activity to this extract. Several tests and qualitative precipitation specific staining for major classes of secondary metabolites were used. Antioxidant capacityin vitrotests (DPPH and ABTS) were also used to assess the extract’s ability to sequester free radicals of 70% hydroethanolic and aqueous extracts of cubiu flour. Alkaloids, organic acids, phenols, flavonoid glycosides, and coumarins were found in the hydroethanolic extract while the aqueous extract presented anthocyanins, gums, tannins and mucilage, amino groups, and volatile and fixed acids. Forin vitrotests, theIC50value obtained in the DPPH assay was 606.3 ± 3.5 μg/mL while that for the ABTS assay was 290.3 ± 10.7 µg/mL. Although cubiu extracts present chemical compounds directly related to antioxidant activity, our results show that it has a low antioxidant activity. Additional studies will be needed to isolate and characterize specific compounds to further assess antioxidant activity.

scholarly journals Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
M Miftahudin ◽  
Rini Hasibuan ◽  
Tatik Chikmawati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Sod Activity ◽  
Different Doses

Abstract. Miftahudin, Hasibuan RS, Chikmawati T. 2019. Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia. Biodiversitas 20: 3715-3722. Three Selaginella species, S. ornata, S. plana, and S. willdenowii, from Java Island, Indonesia, have been known to have antioxidant properties; however, in vivo antioxidant activities of these species have not been reported. This research aimed to evaluate the in vivo antioxidant activity of ethanolic extract of three Selaginella species. The 70% ethanol extract of three Selaginella species at four different doses was administered to mice one day before being treated with oxidative stress. The liver tissue of mice treated with or without oxidative stress was analyzed their lipid peroxidation by measuring MDA concentration and Superoxide Dismutase (SOD) activities. The results showed that there were variations in antioxidant activity among the three Selaginella species. In general, the dose of 0.3 g extract kg-1 BW has been able to reduce lipid peroxidation and increase SOD activity. The administration of S. ornata extract to the mice at 1.2 g extract kg-1 BW reduced the MDA concentration to the lowest level, but the same dose of two other Selaginella extracts caused toxic effects in mice. The antioxidant activities of S. ornata and S. plana were better than that of S. willdenowii extract, and among those species, S. ornata has the best antioxidant activity.

scholarly journals Antioxidant Activities, Total Phenolic Compound And Pigment Contents of Tropical Sargassum sp. Extract, Macerated In Different Solvents Polarity

2019 ◽  
Vol 22 (1) ◽  
pp. 73 ◽  
Author(s):  
Jelita Rahma Hidayati ◽  
Ervia Yudiati ◽  
Delianis Pringgenies ◽  
Zaenal Arifin ◽  
Diah Tri OktaviyantI
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Antiradical Activity ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Phenolic Compound ◽  
Pigment Contents ◽  
Chlorophyll A Content

Exposure of sunlight lead tropical Sargassum sp. to maintain their growth and moreover to bring up their secondary metabollite for life struggling. Sargassum sp. has bioactive compounds that has a potential antioxidant acitivity such as phenolic compounds as well as chlorophyll and carotenoids. This research was conducted to determine antioxidant activities, phenolic compound and pigmens of Sargassum sp. with different solvent that have different polarities. Sample was macerate with n-heksana, ethyl acetate, methanol and aquadest. All the parameters were done spectrophotometrically. IC50 was used to determine the antioxidant activity by antiradical scavenging activity using DPPH (515 nm). Total phenolic compound were tested by Folin-Ciocalteu solution  and used gallic acid as standard (725 nm). The chlorophylls a content were measured at wavelength 662 nm and 645 nm and carotenoids were measured at wavelength 470 nm. The results showed best IC50 is achieved by aquadest extract (72.95 ±0.22 ppm). The highest Total phenolic compound is achieved by ethyl acetat extract (120.29 ±0,404 mg GAE/g sample). The highest chlorophyll a content is achieved by ethyl acetat extract (18.23 ± 0,049 mg/g sample) and the highest carotenoid content is achieved by ethyl acetat extract extract (60.65 ±0,008  µmol/g sample). It can be concluded that aquadest extract can be categorized as the strong antioxidant and antiradical activity, ethyl acetat as a medium antioxidant activity. The simple and save methods of aquadest extract promising that Sargassum  sp. frorn Indonesia is a good candidate compoud for nutraceutical and cosmeceutical approach.  

scholarly journals Antioxidant and Ethylene-related Changes in `Chandler' Strawberry Fruit as Influenced by Maturity

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 807A-807
Author(s):  
Floyd M. Woods* ◽  
William A. Dozier ◽  
Robert C. Ebel ◽  
David G. Himelrick ◽  
Cecilia Mosjidis ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Enzyme Activity ◽  
Antioxidant Activities ◽  
Thiobarbituric Acid ◽  
Membrane Lipid ◽  
Strawberry Fruit ◽  
Signal Molecule ◽  
Stage Of Development ◽  
Fruit Pericarp

The relationship between fruit maturation and accumulation of hydrogen peroxide (H2 O2), lipid peroxidation, ethylene (C2 H4) production, antioxidant activity (hydrophilic, lipophilic and total) and the antioxidant enzyme ascorbate peroxidase (APX, EC 1.11.1.11) in fruit pericarp tissue of `Chandler' (Fragaria × ananassa Duch.) strawberry were measured. `Chandler' fruit pericarp maturation and ripening were accompanied by a decline in H2 O2 content early in fruit development followed by a rapid accumulation. An increase in membrane lipid peroxidation (thiobarbituric acid reactive substances, TBARS) coincided with accumulation of H2 O2, which preceded a rise in C2 H4 production. In general, antioxidant activity declined as fruit matured and ripened. APX enzyme activity increased by 2-fold and peaked at the pink stage of development and then gradually declined with ripening. H2 O2 may serve as a signal molecule to initiate the cascade of oxidative processes during maturation and ripening. APX enzyme activity during maturation and ripening was not substantial and thus, may not have a role in alleviating accumulation of H2 O2 and subsequent events related to oxidative senescence in fruit pericarp. To our knowledge, this is the first study to present fractionated antioxidant activities (HAA, LAA and TAA) from strawberry pericarp as assessed by the ABTS∼+ radical cation assay. A fundamental understanding of the mechanisms involved in the senescent related-oxidative changes during strawberry fruit ontogeny in relation to quality and nutrition is discussed.

scholarly journals Antioxidant activities of aqueous extracts from nine different rose cultivars

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Hamadia Khurshid ◽  
Syed Mubashar Sabir ◽  
Shahid Iqbal Awan ◽  
Syed Rizwan Abbas ◽  
Muhammad Irshad
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Dpph Radical ◽  
Rose Petals

Rose petals have been applied as food additives in teas, cakes and flavor extracts. The aim of this research study was to explore and reveal the antioxidant potential of aqueous extracts of rose petals belonging to nine genotypes of rose (wild as well as hybrid). The in vitro antioxidant activities of roses were studied by lipid peroxidation assay, DPPH radical scavenging assay, iron chelation assay, phosphomolybdenum reduction assay and total phenolic and flavonoid contents. The aqueous extract showed inhibition against lipid peroxidation (TBARS), induced by prooxidants (10 µM FeSO4) in mice liver homogenate. The free radical scavenging activities of the extracts were determined by scavenging of the DPPH radical. Extracts also showed metal chelating activities and high antioxidant activity in the phosphomolybdenum assay. The high content of phenolics and flavonoids detected in aqueous extracts may be responsible for the antioxidant activity. Amongst the different rose genotypes, screened, Rosa moschata (musk rose) was found to carry slightly higher antioxidant potential, owing to its higher phytochemical content.

scholarly journals Effect of Different Cooking Methods on Polyphenols, Carotenoids and Antioxidant Activities of Selected Edible Leaves

Antioxidants ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 117 ◽  
Author(s):  
K. Gunathilake ◽  
K. Ranaweera ◽  
H. Rupasinghe
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Leafy Vegetables ◽  
Cooking Methods ◽  
Oxygen Scavenging ◽  
Total Antioxidant ◽  
The Impact ◽  
Dpph Radical Scavenging

This study aimed to evaluate the effect of cooking (boiling, steaming, and frying) on polyphenols, flavonoids, carotenoids and antioxidant activity of six edible leaves. The total antioxidant capacity of the fresh and cooked leaves was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and singlet oxygen scavenging assays. The results revealed that frying caused a reduction in major bioactives and antioxidant activities in all leafy vegetables tested. However, steamed and boiled leaves of C. auriculata and C. asiatica have shown greater levels of polyphenols, flavonoids, and antioxidant capacity compared with fresh leaves. Polyphenol and flavonoid contents of boiled S. grandiflora and G. lactiferum were higher than that of their fresh form. Boiled and steamed O. zeylanica and S. grandiflora have shown higher carotenoids. Boiled and steamed leaves of P. edulis have shown higher antioxidant activity. The impact of cooking on the changes in bioactive concentrations and antioxidant capacities are dependent on the species and the method of cooking.

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