scholarly journals Promotion of Wound Healing and Prevention of Frostbite Injury in Rat Skin by Exopolysaccharide from the Arctic Marine Bacterium Polaribacter sp. SM1127

Marine Drugs ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 48 ◽  
Author(s):  
Mei-Ling Sun ◽  
Fang Zhao ◽  
Xiu-Lan Chen ◽  
Xi-Ying Zhang ◽  
Yu-Zhong Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Injury Prevention ◽  
Marine Bacterium ◽  
Skin Wound ◽  
Dermal Fibroblasts ◽  
The Arctic ◽  
Good Effect ◽  
Rat Skin ◽  
Skin Wound Healing ◽  
Sd Rats

Many marine microorganisms synthesize exopolysaccharides (EPSs), and some of these EPSs have been reported to have potential in different fields. However, the pharmaceutical potentials of marine EPSs are rarely reported. The EPS secreted by the Artic marine bacterium Polaribacter sp. SM1127 has good antioxidant activity, outstanding moisture-retention ability, and considerable protective property on human dermal fibroblasts (HDFs) at low temperature. Here, the effects of SM1127 EPS on skin wound healing and frostbite injury prevention were studied. Scratch wound assay showed that SM1127 EPS could stimulate the migration of HDFs. In the full-thickness cutaneous wound experiment of Sprague–Dawley (SD) rats, SM1127 EPS increased the wound healing rate and stimulated tissue repair detected by macroscopic observation and histologic examination, showing the ability of SM1127 EPS to promote skin wound healing. In the skin frostbite experiment of SD rats, pretreatment of rat skin with SM1127 EPS increased the rate of frostbite wound healing and promoted the repair of the injured skin significantly, indicating the good effect of SM1127 EPS on frostbite injury prevention. These results suggest the promising potential of SM1127 EPS in the pharmaceutical area to promote skin wound healing and prevent frostbite injury.

scholarly journals Pigment epithelium-derived factor as a multifunctional regulator of wound healing

2015 ◽  
Vol 309 (5) ◽  
pp. H812-H826 ◽  
Author(s):  
Mateusz S. Wietecha ◽  
Mateusz J. Król ◽  
Elizabeth R. Michalczyk ◽  
Lin Chen ◽  
Peter G. Gettins ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Pigment Epithelium ◽  
Skin Wound ◽  
Dermal Fibroblasts ◽  
Tissue Homeostasis ◽  
Skin Wound Healing ◽  
Pigment Epithelium Derived Factor ◽  
Vessel Regression ◽  
Treatment Of Wounds

During dermal wound repair, hypoxia-driven proliferation results in dense but highly permeable, disorganized microvascular networks, similar to those in solid tumors. Concurrently, activated dermal fibroblasts generate an angiopermissive, provisional extracellular matrix (ECM). Unlike cancers, wounds naturally resolve via blood vessel regression and ECM maturation, which are essential for reestablishing tissue homeostasis. Mechanisms guiding wound resolution are poorly understood; one candidate regulator is pigment epithelium-derived factor (PEDF), a secreted glycoprotein. PEDF is a potent antiangiogenic in models of pathological angiogenesis and a promising cancer and cardiovascular disease therapeutic, but little is known about its physiological function. To examine the roles of PEDF in physiological wound repair, we used a reproducible model of excisional skin wound healing in BALB/c mice. We show that PEDF is abundant in unwounded and healing skin, is produced primarily by dermal fibroblasts, binds to resident microvascular endothelial cells, and accumulates in dermal ECM and epidermis. PEDF transcript and protein levels were low during the inflammatory and proliferative phases of healing but increased in quantity and colocalization with microvasculature during wound resolution. Local antibody inhibition of endogenous PEDF delayed vessel regression and collagen maturation during the remodeling phase. Treatment of wounds with intradermal injections of exogenous, recombinant PEDF inhibited nascent angiogenesis by repressing endothelial proliferation, promoted vascular integrity and function, and increased collagen maturity. These results demonstrate that PEDF contributes to the resolution of healing wounds by causing regression of immature blood vessels and stimulating maturation of the vascular microenvironment, thus promoting a return to tissue homeostasis after injury.

Effect of ultraviolet therapy on rat skin wound healing

1990 ◽  
Vol 48 (1) ◽  
pp. 68-71 ◽  
Author(s):  
Isto Nordback ◽  
Rauno Kulmala ◽  
Markku Järvinen
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Rat Skin ◽  
Skin Wound Healing

scholarly journals Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing

2017 ◽  
Author(s):  
Jian‑Li Dong ◽  
Hai‑Cao Dong ◽  
Liang Yang ◽  
Zhe‑Wen Qiu ◽  
Jia Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Rat Skin ◽  
Skin Wound Healing

In-vivo monitoring rat skin wound healing using nonlinear optical microscopy

2014 ◽  
Author(s):  
Jing Chen ◽  
Chungen Guo ◽  
Fan Zhang ◽  
Yahao Xu ◽  
Xiaoqin Zhu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Skin Wound ◽  
Rat Skin ◽  
Skin Wound Healing ◽  
Nonlinear Optical Microscopy ◽  
In Vivo Monitoring

Full-Thickness Skin Wound Healing Using Autologous Keratinocytes and Dermal Fibroblasts with Fibrin

2014 ◽  
Vol 27 (4) ◽  
pp. 171-180 ◽  
Author(s):  
Ruszymah Hj Idrus ◽  
Mohd Adha bin P Rameli ◽  
Kiat Cheong Low ◽  
Jia Xian Law ◽  
Kien Hui Chua ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Dermal Fibroblasts ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Atropa Belladonna L. Water Extract: Modulator of Extracellular Matrix Formation in Vitro and in Vivo

2012 ◽  
pp. 241-250 ◽  
Author(s):  
P. GÁL ◽  
T. VASILENKO ◽  
I. KOVÁČ ◽  
M. KOSTELNÍKOVÁ ◽  
J. JAKUBČO ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Dermal Fibroblasts ◽  
Histological Evaluation ◽  
Control Group ◽  
Atropa Belladonna ◽  
Skin Wound Healing ◽  
Collagen Iii

Previously, we found that treatment of cutaneous wounds with Atropa belladonna L. (AB) revealed shortened process of acute inflammation as well as increased tensile strength and collagen deposition in healing skin wounds (Gál et al. 2009). To better understand AB effect on skin wound healing male Sprague-Dawley rats were submitted to one round full thickness skin wound on the back. In two experimental groups two different concentrations of AB extract were daily applied whereas the control group remained untreated. For histological evaluation samples were removed on day 21 after surgery and stained for wide spectrum cytokeratin, collagen III, fibronectin, galectin-1, and vimentin. In addition, in the in vitro study different concentration of AB extract were used to evaluate differences in HaCaT keratinocytes proliferation and differentiation by detection of Ki67 and keratin-19 expressions. Furthermore, to assess ECM formation of human dermal fibroblasts on the in vitro level fibronectin and galectin-1 were visualized. Our study showed that AB induces fibronectin and galectin-1 rich ECM formation in vitro and in vivo. In addition, the proliferation of keratinocytes was also increased. In conclusion, AB is an effective modulator of skin wound healing. Nevertheless, further research is needed to find optimal therapeutic concentration and exact underlying mechanism of action.

scholarly journals Expression of Matrix Metalloproteinases during Rat Skin Wound Healing: Evidence that Membrane Type-1 Matrix Metalloproteinase Is a Stromal Activator of Pro-Gelatinase A

1997 ◽  
Vol 137 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Akiko Okada ◽  
Catherine Tomasetto ◽  
Yves Lutz ◽  
Jean-Pierre Bellocq ◽  
Marie-Christine Rio ◽  
...  
Keyword(s):  
Wound Healing ◽  
Connective Tissue ◽  
Matrix Metalloproteinase ◽  
Tissue Distribution ◽  
Skin Wound ◽  
Gelatinase A ◽  
Rat Skin ◽  
Skin Wound Healing ◽  
Membrane Type

Skin wound healing depends on cell migration and extracellular matrix remodeling. Both processes, which are necessary for reepithelization and restoration of the underlying connective tissue, are believed to involve the action of extracellular proteinases. We screened cDNA libraries and we found that six matrix metalloproteinase genes were highly expressed during rat skin wound healing. They were namely those of stromelysin 1, stromelysin 3, collagenase 3, gelatinase A (GelA), gelatinase B, and membrane type-1 matrix metalloproteinase (MT1-MMP). The expression kinetics of these MMP genes, the tissue distribution of their transcripts, the results of cotransfection experiments in COS-1 cells, and zymographic analyses performed using microdissected rat wound tissues support the possibility that during cutaneous wound healing pro-GelA and pro-gelatinase B are activated by MT1-MMP and stromelysin 1, respectively. Since MT1-MMP has been demonstrated to be a membrane-associated protein (Sato, H., T. Takino, Y. Okada, J. Cao, A. Shinagawa, E. Yamamoto, and M. Seiki. 1994. Nature (Lond.). 370: 61–65), our finding that GelA and MT1-MMP transcripts were expressed in stromal cells exhibiting a similar tissue distribution suggests that MT1-MMP activates pro-GelA at the stromal cell surface. This possibility is further supported by our observation that the processing of proGelA to its mature form correlated to the detection of MT1-MMP in cell membranes of rat fibroblasts expressing the MT1-MMP and GelA genes. These observations, together with the detection of high levels of the mature GelA form in the granulation tissue but not in the regenerating epidermis, suggest that MT1-MMP and GelA contribute to the restoration of connective tissue during rat skin wound healing.

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