scholarly journals Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters

Marine Drugs ◽  
2019 ◽  
Vol 17 (8) ◽  
pp. 449 ◽  
Author(s):  
Magnús Örn Stefánsson ◽  
Sigurður Baldursson ◽  
Kristinn P. Magnússon ◽  
Arnheiður Eyþórsdóttir ◽  
Hjörleifur Einarsson

The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A ( 2 . 10 ± 0 . 70 g/L) and B ( 1 . 54 ± 0 . 17 g/L) produced more biomass than the ones isolated from Location C ( 0 . 43 ± 0 . 02 g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine ( 2 . 55 ± 0 . 74 g/L) as compared to of commercial origin (  1 . 06 ± 0 . 57 g/L). Glycerol ( 2 . 43 ± 0 . 56 g/L) was a better carbon source than glucose ( 1 . 84 ± 0 . 57 g/L) in growth studies. Fatty acid (FA) profiles showed that the isolates from Location C (S. minutum) had low ratios of monounsaturated ( 4 . 21 ± 2 . 96 % ) and omega-6 ( 0 . 68 ± 0 . 59 % ) FAs. However, the isolates also had high ratios of docosahexaenoic acid (DHA; 35 . 65 ± 1 . 73 % ) and total omega-3 FAs ( 40 . 39 ± 2 . 39 % ), indicating that they could serve as a source of marine oils for human consumption and in aquaculture feeds. The T. kinnei isolates from Location A could be used in biodiesel production due to their high ratios of monounsaturated ( 18 . 38 ± 6 . 27 % ) long chain ( 57 . 43 ± 8 . 27 % ) FAs.

2021 ◽  
Vol 11 (24) ◽  
pp. 11586
Author(s):  
Bruna Marques ◽  
Elisabete Maciel ◽  
Maria Rosário Domingues ◽  
Ricardo Calado ◽  
Ana Isabel Lillebø

Halophytes have gradually been introduced in marine integrated multi-trophic aquaculture (IMTA) systems due to their capacity to bioremediate nutrient-rich marine effluents and their potential use for human consumption due to their content of omega-3 and omega-6 fatty acids (FA). To foster the valorization of halophytes produced using an IMTA framework for human consumption, it is important that culture conditions keep or enhance their FA profile, when compared to that displayed by conspecifics in the wild. The main objective of the present study was to compare the FA profiles of three halophyte species (Halimione portulacoides, Salicornia ramosissima and Sarcocornia perennis) cultured in aquaponics coupled to an IMTA system with that of wild conspecifics retrieved from donor sites. The FA profiles were compared considering different plant organs (edible parts and roots) and sampling dates (spring, summer and autumn). Results show that the FA profiles of specimens cultured in aquaponics were significantly different from that of wild conspecifics, displaying a high content of omega-3 FAs in edible parts, particularly during summer, and mostly in the form of α-linolenic acid (ALA, 18:3n-3). In more detail, for the specimens cultured in aquaponics, ALA concentration in the edible parts of each species ranged from 5.10 to 7.11 μg mg−1 DW in H. portulacoides, from 5.66 to 9.19 μg mg−1 DW in S. ramosissima and from 5.49 to 7.20 μg mg−1 DW in S. perennis. Concerning the omega-6 linoleic acid (LA, 18:2n-6) identified in edible parts, the concentrations ranged from 2.25 to 2.46 μg mg−1 DW in H. portulacoides, from 3.26 to 4.84 μg mg−1 DW in S. ramosissima, and from 2.17 to 3.06 μg mg−1 DW in S. perennis. The nutritional quality was assessed through the ratio of PUFA/SFA, for both wild and cultured plants, and revealed values well above the threshold (0.45), the threshold value indicative of good nutritional quality. Overall, the culture conditions tested in the present work reinforce the potential of aquaponics coupled to marine IMTA to produce high-quality halophytes suitable for human consumption.


PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246357
Author(s):  
Mauricio X. S. Oliveira ◽  
Andre S. V. Palma ◽  
Barbara R. Reis ◽  
Camila S. R. Franco ◽  
Alessandra P. S. Marconi ◽  
...  

Fluid milk and its derivatives are important dietary ingredients that contribute to daily nutrient intake of the modern Homo sapiens. To produce milk that is healthier for human consumption, the present study evaluated the effect of adding soybean oil and linseed oil in the diet of lactating cows. The fatty acid profile of milk, milk composition, and the blood parameters of cows were evaluated. Eighteen Holstein cows were distributed in a replicated Latin square design and distributed according to the following treatments: 1) Control (CC): traditional dairy cow diet, without addition of oil; 2) Soybean oil (SO): 2.5% addition of soybean oil to the traditional diet, as a source of omega-6; 3) Linseed oil (LO): 2.5% addition of linseed oil in the diet as a source of omega-3. Milk production was not affected, but oil supplementation decreased feed intake by 1.93 kg/cow/day. The milk fat percentage was significantly lower when cows were supplemented with vegetable oil (3.37, 2.75 and 2.89% for CC, SO and LO, respectively). However, both soybean and linseed oils decreased the concentration of saturated fatty acids (66.89, 56.52 and 56.60 g/100g for CC, SO and LO respectively), increased the amount of unsaturated fatty acids in milk (33.05, 43.39, and 43.35 g/100g for CC, SO and LO respectively) and decreased the ratio between saturated/unsaturated fatty acids (2.12, 1.34, and 1.36 for CC, SO and LO respectively). Furthermore, SO and LO increased significantly the concentration of monounsaturated fatty acids (29.58, 39.55 and 39.47 g/100g for CC, SO and LO respectively), though it did not significantly alter the level of polyunsaturated fatty acids in milk fat (3.57, 3.93 and 3.98 g/100g for CC, SO and LO respectively). Supplementation with LO enhanced the concentration of omega-3 fatty acids on milk (0.32, 0.36, and 1.02 for CC, SO and LO respectively). Blood variables aspartate aminotransferase, gamma glutamyl transferase, urea, albumin, creatinine and total proteins were not altered. On the other hand, total cholesterol, HDL and LDL were greater in the group supplemented with vegetable oils. Supplementation with vegetable oils reduced the dry matter intake of cows, the fat content of milk, and improved saturated/unsaturated fatty acid ratio of milk fat. Compared to the SO treatment, animals fed LO produced milk with greater content of omega-3, and a more desirable omega-6/omega-3 ratio on a human nutrition perspective. Thus, the inclusion of SO and LO in the diet of lactating dairy cows makes the milk fatty acid profile nutritionally healthier for the human consumption.


2018 ◽  
Vol 69 (11) ◽  
pp. 2973-2980 ◽  
Author(s):  
Candice Popescu (Popiniuc) ◽  
Carmen Popescu ◽  
Stefan Manea ◽  
Valentin Vladut ◽  
Ioan Caba ◽  
...  

Fatty grass, grasita or purslane (Portulaca oleracea L.) is an important plant identified as a natural weed in field crops and lawns. Purslane is widespread worldwide and is popular as edible plants in many areas of Europe, Asia and the Mediterranean region. This plant possesses mucilaginous substances of great medicinal importance. It is a rich source of potassium, magnesium and calcium and has the potential to be used as a vegetal source of omega-3 and omega-6. It is very good as a source of alpha-linolenic acid (ALA) and gamma-linolenic acid (GLA) and contains the highest amount compared to any other green leafy vegetables. It also contains significant amounts of alpha-tocopherol and ascorbic acid, as well as an increased content of oxalate in fresh leaves. The antioxidant value and nutritional content of purslane are important for human consumption. It reveals a huge nutritional potential and indicates the potential for future use of this plant in food industry, dietary supplements, drugs, cosmetics and products for body care.


2009 ◽  
Vol 87 (1) ◽  
pp. 305-317
Author(s):  
MOHAMED Y. EL-HAWARY ◽  
ELSAUD A. MOHAMED ◽  
MOUSA A. SALEM ◽  
SOAD M.H. REFAAT
Keyword(s):  
Omega 3 ◽  
Omega 6 ◽  

2018 ◽  
Vol 3 (2) ◽  

A new study is providing further evidence about the potentially life-threatening danger of drinking soda on a daily basis. Large, long-running epidemiological studies have also concluded that there is indeed a link between the foods a person eats and his risk of colon cancer. During this process, the bacteria produce compounds such as secondary bile acids that have been shown to cause inflammation and cancer in lab studies. A study by the University of Aberdeen has found first time that a higher concentration of the molecules that breakdown omega-3 fatty acids is associated with a higher chance of survival from bowel cancer. Results showed that a higher proportion of omega-3 metabolising enzyme to omega-6 metabolising enzyme is associated with less spread of the tumor and a greater chance of survival for an individual patient. Cancer stem cells can be put into hibernation by a little-known drug diphenyleneiodonium (DPI) according to researchers from the University of Salford, UK. DPI effectively switches off the stem cancer cells, preventing their proliferation. It was showed that as the number of mutant KRAS copies increases, the tumor’s aggressiveness and ability to metastasize also increases. Disruption of endogenous protective mechanisms determines the evolution of cancer.


2020 ◽  
Vol 9 (7) ◽  
pp. e334974037
Author(s):  
Graciele Araújo de Oliveira Caetano ◽  
Angela Aparecida da Fonseca ◽  
Cibelle Borges Figueiredo

Um fator diferencial na produção de bovinos é a compreensão da contribuição individual dos ácidos graxos e do papel dos lipídeos nos ruminantes, tanto na forma de análise (ácido graxo ou extrato etéreo), conceitos relacionados às características de ômega 3, ômega 6 e ácido linoleico conjugado na bio-hidrogenação, no metabolismo pós-absorção, bem como no conteúdo e composição dos ácidos graxos em relação à matéria seca dos alimentos. Sabe-se que diversos fatores podem afetar o teor, digestibilidade e degradabilidade das fibras presente na forragem, e o mesmo ocorre com os lipídeos. As diferenças entre os valores dos compostos presentes nos lipídeos podem ocorrer dependendo do ambiente, região do país ou fase de colheita e por isso, torna-se importante obter a análise da composição de ácidos graxos dos alimentos lipídicos, para que dessa maneira seja possível otimizar a utilização deste nutriente na dieta de animais ruminantes. Sendo assim, a proposta dessa revisão será trazer a discussão sobre o tema e abordar um entendimento sobre o conteúdo e composição de ácidos graxos dos ingredientes que compõem a dieta dos animais ruminantes, com ênfase na influência exercida no desempenho produtivo, imunonutrição, e no produto final (carne ou leite).


2020 ◽  
Vol 3 (1) ◽  
pp. 10
Author(s):  
Muhammad Noor Yasin ◽  
Firlianty . ◽  
Anang Najamudin
Keyword(s):  
Omega 3 ◽  
Omega 6 ◽  

Eccado adalah produk olahan yang dibuat dari daging atau udang yang dicincang dengan penambahan tepung dan bumbu-bumbu. Pada umumnya Eccado diolah dari daging ayam, untuk menggantikan eccado ayam maka dicari sumber alternatif baru salah satunya digunakan ikan Gabus karena memiliki daging yang banyak, tebal dan putih. Potensi eccado ikan Gabus ini sangat tinggi baik aspek ekonomi juga kandungan gizinya karena mengandung omega 3 dan omega 6. Ikan Gabus bisa dimanfaatkan dengan cara langsung mengolahnya menjadi beberapa jenis makanan atau mengawetkannya melalui proses penggaraman dan pengeringan. Masih belum banyaknya diversifikasi bentuk pengolahan ikan hasil usaha budidaya khususnya ikan Gabus menjadi makanan olahan lain juga disebabkan karena masih minimnya informasi tentang teknologi pengolahan hasil perikanan. Permasalahan yang dihadapi mitra adalah : 1) Selama ini mitra belum mengetahui variasi tentang Teknologi Hasil Perikanan karena terbatasnya pengetahuan dan informasi yang diterima serta keterampilan yang dimiliki. 2) Mitra juga belum pernah mendapatkan pelatihan dan demonstrasi tentang pembuatan eccado dari ikan air tawar dalam hal ini ikan jenis ikan family Channidae Menurut SNI 7756 : 2013 dapat didefinisikan Eccado ikan merupakan produk olahan hasil perikanan dengan menggunakan lumatan daging ikan/udang dan atau surimi minimum 30%, tepung dan bahan-bahan lainnya, dibentuk dan dibungkus dengan kulit pangsit. Sedangkan Eccado merupakan salah satu jenis olahan fish jelly produk dengan menggunakan kulit kembang tahu yang berfungsi memberikan rasa yang khas.Tujuan kegiatan pengabdian kepada masyarakat ini untuk membantu mengatasi permasalahan yang dihadapi oleh masyarakat Pahandut Seberang l) Pendampingan teknologi diversifikasi pengolahan daging ikan menjadi eccado. 2) Promosi kegiatan Pahandut seberang sebagai lokasi wisata kuliner dengan kegiatan-kegiatan yang dapat meningkatkan kualitas kuliner dari ikan dan ekonomi masyarakat di sekitarnya.


2014 ◽  
Vol 4 (1) ◽  
pp. 31-39
Author(s):  
Siwitri Kadarsih

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords : 


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