scholarly journals Isolation and Characterisation of Major and Minor Collagens from Hyaline Cartilage of Hoki (Macruronus novaezelandiae)

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 223 ◽  
Author(s):  
Cumming ◽  
Hall ◽  
Hofman
Keyword(s):  
Thermal Denaturation ◽  
Collagen Type ◽  
Hyaline Cartilage ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Salt Precipitation ◽  
Nasal Cartilage ◽  
Sds Page ◽  
Native Collagen ◽  
First Time

The composition and properties of collagen in teleost (bony fish) cartilage have never been studied. In this study, we aimed to identify and characterise all collagen species in the nasal cartilage of hoki (Macruronus novaezelandiae). Four native collagen species were extracted using two techniques, and isolated with differential salt precipitation. We were able to assign the identity of three of these collagen species on the basis of solubility, SDS-PAGE and amino acid analyses. We found that hoki cartilage contains the major collagen, type II, and the minor collagens, type IX and type XI, which are homologous to those found in mammal and chicken cartilage. Using these extraction protocols, we also isolated a full-length type IX collagen from cartilage for the first time. In addition, we detected a 90 kDa, highly glycosylated collagen that has not been identified in any other species. For each isolate, structural and biochemical characterisations were performed using circular dichroism and Fourier transform infrared spectroscopy analyses, and the thermal denaturation properties were determined. Our results showed that the properties of hoki cartilage-derived collagens are similar to those of collagens in mammalian cartilage, indicating that teleost cartilage could provide biological ingredients for the development of biomaterials to treat cartilage-related illnesses.

POTENCY OF ORAL UN-DENATURED TYPE II COLLAGENIN THE TREATMENT OF OSTEOARTHRITIS, WITH SPECIFICAL RELATIONSHIP TO KNEE JOINT- A META-ANALYTIC REVIEW

2021 ◽  
pp. 6-10
Author(s):  
R K Guhan ◽  
Saran Karthik.S ◽  
Ashwin V Y ◽  
Venkatachalam. K ◽  
Yokesh M ◽  
...  
Keyword(s):  
Knee Joint ◽  
Chondroitin Sulfate ◽  
Collagen Type ◽  
Joint Disease ◽  
Primary Data ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Number Of Patients ◽  
Analytic Review ◽  
Native Collagen

Introduction: One of the chronic progressive diseases of the elderly is Osteoarthritis. There is a wide spectrum of Nutraceuticals, for Osteoarthritis, but there does not exist, a convincing literature based evidence, in support of their denitive and specic rationale of utility. We here in, aim to evaluate the evidence in literature hither to available, for establishing the potency and efcaciousness of the indigenous type II collagen variant. Methods: st st A schematic search was performed of Pub Med, Scopus and the Google Scholar, from dates (1 December 2009 to 1 December 2020), with the search terms: 'Osteoarthritis', 'Nutraceuticals', 'Oral Collagen', 'Glucosamine', 'Chondroitin Sulfate', 'Acetaminophen' and 'Native Collagen'. Articles containing the following were included in the study: Randomized Control Trial and Clinical Trial, Primary data, OA and Oral Collagen studies related to joint disease. Total number of patients studied, the number of patients who were treated by Native Collagen Type II variant, Denatured Collagen Type II variant, Glucosamine, Chondroitin Sulfate and Acetaminophen.A number of studies using various scoring systems were incorporated in our study. Finally, all the functional outcomes, according to the VAS and WOMAC scores, were cumulatively tabulated, and analyzed schematically and their results deduced. Results: Multiple researches have been executed, to elucidate upon the efcacy and the safety, of Oral Collagen of the type II variant, in the medicaments prescribed for OA, especially relating to the Knee joint. Oral Collagen is administered, either in a Denatured or an Undenatured form .The results indicate , that out of all the Nutraceuticals, Undenatured/Native collagen of the type II variant, proved to be by far the most safe and signicantly more efcacious, compared to other Nutraceuticals. Although all the suggested treatments reduced the WOMAC and the VAS scores, here in UC II, showed more efciency and sustenance of the pain reduction, in both the assessment scores, in comparison with other orally administrated Nutraceuticals. Conclusion: Our Meta-Analysis concludes that, Type II Undenatured Collagen, is a relatively safe and also signicantly more efcient, in improving the joint function, ROM and for the alleviation of bone joint pain, in OA knee (Genu OA) patients.

Specificity and T cell receptor β chain usage of a human collagen type II-reactive T cell clone derived from a healthy individual

1992 ◽  
Vol 22 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Tan Yan ◽  
Harald Burkhardt ◽  
Thomas Ritter ◽  
Barbara Bröker ◽  
Karl Heinz Mann ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Collagen Type ◽  
Cell Clone ◽  
Cell Receptor ◽  
Type Ii ◽  
Collagen Type Ii ◽  
T Cell Clone ◽  
Human Collagen ◽  
Β Chain

Localization of pro-collagen type ii mrna and collagen type ii in the healing tooth socket of the rat

1995 ◽  
Vol 40 (3) ◽  
pp. 181-185 ◽  
Author(s):  
H. Devlin ◽  
J. Hoyland ◽  
A.J. Freemont ◽  
P. Sloan
Keyword(s):  
Collagen Type ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Tooth Socket

scholarly journals Role of undenatured collagen type ii in management of osteoarthritis: A hospital based study

2020 ◽  
Vol 6 (4) ◽  
pp. 747-750
Author(s):  
Dr. Sathik Babu ◽  
Dr. Pradeep Elangovan ◽  
Dr. Dinesh Kumar S
Keyword(s):  
Collagen Type ◽  
Type Ii ◽  
Collagen Type Ii

scholarly journals The Coll2-1 peptide of collagen type II: a new actor of synovitis in osteoarthritis

2018 ◽  
Vol 26 ◽  
pp. S120-S121 ◽  
Author(s):  
C. Lambert ◽  
D. Borderie ◽  
F. Rannou ◽  
Y. Henrotin
Keyword(s):  
Collagen Type ◽  
Type Ii ◽  
Collagen Type Ii
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