scholarly journals A New Sesquiterpenoid Aminoquinone from an Indonesian Marine Sponge

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 158 ◽  
Author(s):  
Walter Balansa ◽  
Ute Mettal ◽  
Zerlina Wuisan ◽  
Anuchit Plubrukarn ◽  
Frans Ijong ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Data Analysis ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Micrococcus Luteus ◽  
2D Nmr ◽  
Cytotoxic Effects ◽  
Sar Studies ◽  
Structure Activity

Sponges are a well-known bioresource for bioactive compounds. In this study, antibacterial activity-guided fractionation of the extract from an Indonesian marine Dactylospongia elegans sponge led to the discovery of four merosesquiterpenoids, namely, a new sesquiterpenoid aminoquinone nakijiquinone V (1), along with illimaquinone (2), smenospongine (3), and dyctioceratine C (4). The structure of compound 1 was elucidated by 1D and 2D NMR as well as by LC-HRESIMS data analysis. Compounds 2–4 showed moderate to low antimicrobial activity against Bacillus megaterium DSM32 with a minimum inhibitory concentration (MIC) of 32 μg/mL, 32 μg/mL, and 64 μg/mL, respectively. Furthermore, compounds 2 and 3 both inhibited Micrococcus luteus ATCC 4698 with a MIC of 32 μg/mL. In conclusion, the isolated merosesquiterpenoids, which are known for their cytotoxic effects, showed antibacterial activity and prompt future structure activity relationship (SAR) studies concerning the various bioactivities observed for this group of natural products.

scholarly journals HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil

2019 ◽  
Vol 84 (12) ◽  
pp. 1355-1365 ◽  
Author(s):  
Jovana Stankovic ◽  
Miroslav Novakovic ◽  
Vele Tesevic ◽  
Ana Ciric ◽  
Marina Sokovic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Activity ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Minor Component ◽  
2D Nmr ◽  
Antibacterial Compounds ◽  
Direct Bioautography

This study was performed to determine the main antibacterial compounds of the essential oil (??) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D- and 2D-NMR and GC?MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.

scholarly journals ANTIMICROBIAL ACTIVITY OF CHIVES AND GINGER EXTRACT ON ESCHERICHIA COLI AND SALMONELLA SPP. ISOLATED FROM BROILER CHICKENS

2019 ◽  
Vol 128 (3B) ◽  
Author(s):  
Phan Vu Hai ◽  
Hoang Thi Hong Van ◽  
Nguyen Van Chao ◽  
Nguyen Dinh Thuy Khuong ◽  
Thuong Thi Thanh Le ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Broiler Chickens ◽  
Inhibitory Concentration ◽  
Salmonella Spp ◽  
Ginger Extract ◽  
E Coli ◽  
Bactericidal Effects

The chives and ginger’s bulbs were extracted by ethanol 96%, 72%, 48% within 5, 10 and 15 days for each concentration (15, 30 and 45 days in total, respectively). The solidified extract then was used for antibacterial activity against E. coli and Salmonella spp. isolated from fecal of chickens with diarrhoea. The results showed that both ginger and chive, which socked and leached for greater than 30 days gave better antibacterial ability. Extracts diluted at concentrations of 5 µg/µl, 7.5 µg/µl and 10 µg/µl of ginger and chive bulbs are resistant to both bacteria. Compared with antibiotics, E. coli was resistant to amoxicillin, whereas Salmonella spp. was resistant to gentamicin and amoxicillin. The minimum inhibitory concentration (MIC) of chives extract (30 days) was 16-63 (31-125) mg/ml and ginger extract (30 days) was 16-80 (2-4) mg/ml; overall, the results indicated that both extract had bacteriostatic/bactericidal effects on E. coli and Salmonella spp.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Umbi Batang Tumbuhan Sarang Semut (Myrmecodia Pendens Merr. & L. M. Perry) Terhadap Pseudomonas Aeruginosa Dan Staphylococcus Aureus

2017 ◽  
Author(s):  
Ardianti Febriana
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Phytochemical Screening ◽  
Gram Positive ◽  
Tetracycline Hcl ◽  
And Staphylococcus Aureus

AbstrakTumbuhan sarang semut (Myrmecodia pendens Merr. & L. M. Perry) secara empiris digunakan sebagai obat yang memiliki aktivitas antibakteri. Namun, belum pernah diteliti mengenai aktivitas antibakteri tersebut. Penelitian ini dilakukan untuk mengetahui aktivitas antibakteri umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) terhadap bakteri Gram negatif dan Gram positif yaitu Pseudomonas aeruginosa dan Staphylococcus aureus, menentukan konsentrasi hambat tumbuh minimum (KHTM), dan nilai banding ak- tivitas antibakterinya terhadap tetrasiklin-HCl. Hasil pengujian aktivitas antibakteri umbi batang sarang semut menunjukkan bahwa ekstrak umbi batang sarang semut memiliki ak- tivitas antibakteri terhadap kedua bakteri tersebut. Konsentrasi hambat tumbuh minimum (KHTM) ekstrak umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) terhadap Pseudomonas aeruginosa terletak pada konsentrasi 0,83 % , sedangkan pada Staph- ylococcus aureus terletak pada konsentrasi 0,8 %. Hasil uji banding ekstrak umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) dengan tetrasiklin-HCl terhadap Pseudomonas aeruginosa dan Staphylococcus aureus berturut-turut sebesar 1 : 6,709 x 10-4 dan 1 : 1,038 x 10-5. Penapisan fitokimia menunjukkan bahwa ekstrak umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) mengandung flavonoid dan polifenol.Kata kunci: Myrmecodia pendens Merr. & L. M. Perry, Aktivitas antibakteri, Pseudomo- nas, StaphylococcusABSTRACTThe ant plant (Myrmecodia pendens Merr. & L. M. Perry) is one of plant applied as tradi- tional medicine. Empirically, the tuber of the ant plant have antibacterial activity. However, the examination of antimicrobial activity from extract of the tuber has never been reported. The research was done to know the activity of antimcrobial againts Gram positive and Gram negatif bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus, to determine Minimum Inhibitory Concentration (MIC) of the tuber, and comparative value antibacterial activity with tetracycline-HCl. The result revealed that the tuber of the ant plant (Myrmeco-dia pendens Merr. & L. M. Perry) has antibacterial activity againts those bacteria. The Min- imum Inhibitory Concentration of extract from the tuber of ant plant to Pseudomonas aeru- ginosa was in range 8,2x103-8,3x103 ppm of concentration, and to Staphylococcus aureus was in range 7,5x103-8x103 ppm of concentration. Comparative value of extract from the tuber of ant plant with tetracycline-HCl againts Pseudomonas aeruginosa and Staphylococ- cus aureus were respective 1:.6.709 x 10-4 and :1.038 x 10-5. Phytochemical screening showed that the extract of the tuber contains flavonoid and polifenol.Keywords: Myrmecodia pendens Merr. & L. M. Perry, Antibacterial activity, Pseudomonas, Staphylococcus

scholarly journals A New Amide From Piper maingayi Hk.F. (Piperaceae)

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1985582 ◽  
Author(s):  
Nur Athirah Hashim ◽  
Farediah Ahmad ◽  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Shamsul Khamis
Keyword(s):  
Antibacterial Activity ◽  
Oleic Acid ◽  
Minimum Inhibitory Concentration ◽  
Methyl Linolenate ◽  
Inhibitory Concentration ◽  
2D Nmr ◽  
Strong Inhibition ◽  
Phytochemical Investigation ◽  
Mic Value

Phytochemical investigation from the leaves and stem extracts of Piper maingayi Hk.F. resulted in the isolation of a new amide, N-isobutyl-15-(3″,4″-methylenedioxyphenyl)-2 E,4 E,12 Z-pentadecatrienamide (1), together with sesamin (2), piperumbellactam A (3), cepharadione A (4), cepharadione B (5), piperolactam A (6), oleic acid (7), methyl linolenate (8), β-sitosterol (9), butyl dodecanoate (10), and isovanillic acid (11). Their structures were established by the analysis of their spectroscopic (1D and 2D NMR) and spectrometric (MS) data, as well as by comparison with those reported in the literature. Compound (1) exhibited strong inhibition against lipoxygenase (15-LOX) at IC50 42.5 μM and antibacterial activity toward Bacillus subtillis and Pseudomas aeruginosa with minimum inhibitory concentration (MIC) value of 250 μg/mL each.

Terpenoids from Atylosia scarabaeoides and their antimicrobial activity

1970 ◽  
Vol 4 (2) ◽  
Author(s):  
M Mukhlesur Rahman ◽  
Proma Khondkar ◽  
Alexandar I Gray
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Petroleum Ether ◽  
Key Words ◽  
Full Text ◽  
Inhibitory Concentration ◽  
Petroleum Ether Extract ◽  
2D Nmr ◽  
Ether Extract ◽  
Atylosia Scarabaeoides

Three terpenes-caryophyllene-4,5-oxide, a-amyrin and b-amyrin were isolated from the petroleum ether extract of Atylosia scarabaeoides. The structures of the compounds were confirmed by a series of 1D and 2D NMR and MS analyses. The minimum inhibitory concentration (MIC) of caryophyllene-4,5-oxide was found to be 50-200 mg/ml. Key words: Atylosia scarabaeoides; Papilionaceae; Caryophyllene-4,5-oxide; Chemotaxonomy; Antimicrobial Dhaka Univ. J. Pharm. Sci. Vol.4(2) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

Synthesis and Antimicrobial activity of Piperazine analogues containing [1, 3, 4] Thiadiazole ring

2021 ◽  
pp. 4710-4714
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Data Structures ◽  
Mass Spectroscopy ◽  
Inhibitory Concentration ◽  
Vibrio Cholera ◽  
Standard Drug ◽  
Thiadiazole Ring

A new series of piperazine-1, 3, 4-thiadiazole has been synthesized, purified and characterized with the help of their analytical and spectral data. Structures of the synthesized compounds were confirmed by IR, 1H NMR and mass spectroscopy. The potential antimicrobial effects of the synthesized compounds were investigated using Vibrio cholera and Bacillus subtilis. The newly synthesized compounds exhibited capable activities against Vibrio cholera and Bacillus subtilis and it showed minimum inhibitory concentration. In this study, few compounds showed appreciable antibacterial activity. The compound PT6 shows highly significant antibacterial and good inhibition as compared to the standard drug.

scholarly journals Evaluation of polysaccharides content in fruit bodies and their antimicrobial activity of four Ganoderma lucidum (W Curt.: Fr.) P. Karst. strains cultivated on different wood type substrates

2012 ◽  
Vol 81 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Krystyna Skalicka-Woźniak ◽  
Janusz Szypowski ◽  
Renata Łoś ◽  
Marek Siwulski ◽  
Krzysztof Sobieralski ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Quantitative Determination ◽  
Ganoderma Lucidum ◽  
Inhibitory Concentration ◽  
Micrococcus Luteus ◽  
Dry Weight ◽  
Bacterial Strains

Quantitative determination of polysaccharides in <em>Ganoderma lucidum </em>fruit bodies from different sawdust cultivation substrates and their antibacterial activity was done. Thirty six samples were analyzed. Four strains of <em>Ganoderma lucidum </em>(GL01, GL02, GL03 and GL04) were cultivated on the growth substrates of three different sawdust types: birch (Bo), maple (Kl) or alder (Ol) amended with wheat bran in three different concentrations: 10, 20 and 30% (w/w). Even though the richest in polysaccharides was GL01 strain, the highest yields of the polysaccharides were determined in GL04Kl3 sample and was 112.82 mg/g of dry weight. The antibacterial activity of polysaccharides was determined in vitro using micro-dilution broth method. The panel of eight reference bacterial strains was used. All the polysaccharide samples tested showed the broad spectrum and the moderate antibacterial activity. <em>Micrococcus luteus </em>ATCC 10240 strain was the most sensitive with <em>MIC </em>(minimal inhibitory concentration) = 0.63 − 1.25 mg/mL.

scholarly journals Composition and antimicrobial activity of the essential oil from Galatella linosyris (L.) Rchb. f. (Asteraceae)

2012 ◽  
Vol 77 (5) ◽  
pp. 619-626 ◽  
Author(s):  
Dejan Godjevac ◽  
Ljubodrag Vujisic ◽  
Ivan Vuckovic ◽  
Vlatka Vajs ◽  
Marina Sokovic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Chemical Analysis ◽  
Essential Oil ◽  
Inhibitory Concentration

The investigation of chemical composition and antimicrobial activity of the essential oil of Galatella linosyris was presented. Chemical analysis (GC/MS, NMR) showed that sabinene (40%), ?-pinene (35.5%), ?-pinene (4.5%), limonene (4%), ?-muurolene (4%), and E-Caryophyllene (3.3%) were dominant components in this oil. The microdilution assays was used to evaluate minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC, MFC). G. linosyris essential oil exhibited better antifungal than antibacterial activity.

Green and Simple Synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia abrotanoides: Characterization, Optimization and Antimicrobial Activity

2020 ◽  
Vol 21 (11) ◽  
pp. 1129-1137 ◽  
Author(s):  
Somayeh Mirsadeghi ◽  
Masoumeh F. Koudehi ◽  
Hamid R. Rajabi ◽  
Seied M. Pourmortazavi
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Minimum Inhibitory Concentration ◽  
Plant Extract ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Extraction Temperature ◽  
Silver Particles ◽  
Silver Nps ◽  
Perovskia Abrotanoides

Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction temperature, on the size of silver particles were considered and optimized. Several analytical methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotometer, XRD and SEM. Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory concentration, minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory concentration/ minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively. Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

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