scholarly journals Desertomycin G, a New Antibiotic with Activity against Mycobacterium tuberculosis and Human Breast Tumor Cell Lines Produced by Streptomyces althioticus MSM3, Isolated from the Cantabrian Sea Intertidal Macroalgae Ulva sp.

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 114 ◽  
Author(s):  
Alfredo F. Braña ◽  
Aida Sarmiento-Vizcaíno ◽  
Ignacio Pérez-Victoria ◽  
Jesús Martín ◽  
Luis Otero ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tumor Cell ◽  
Cell Lines ◽  
Antibiotic Activity ◽  
Breast Adenocarcinoma ◽  
Tumor Cell Lines ◽  
Human Breast ◽  
Antibiotic Resistant ◽  
Clinical Pathogens ◽  
Corynebacterium Urealyticum

The isolation and structural elucidation of a structurally new desertomycin, designated as desertomycin G (1), with strong antibiotic activity against several clinically relevant antibiotic resistant pathogens are described herein. This new natural product was obtained from cultures of the marine actinomycete Streptomyces althioticus MSM3, isolated from samples of the intertidal seaweed Ulva sp. collected in the Cantabrian Sea (Northeast Atlantic Ocean). Particularly interesting is its strong antibiotic activity against Mycobacterium tuberculosis clinical isolates, resistant to antibiotics in clinical use. To the best of our knowledge, this is the first report on a member of the desertomycin family displaying such activity. Additionally, desertomycin G shows strong antibiotic activities against other relevant Gram-positive clinical pathogens such as Corynebacterium urealyticum, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Enterococcus faecium, Enterococcus faecalis, and Clostridium perfringens. Desertomycin G also displays moderate antibiotic activity against relevant Gram-negative clinical pathogens such as Bacteroides fragilis, Haemophilus influenzae and Neisseria meningitidis. In addition, the compound affects viability of tumor cell lines, such as human breast adenocarcinoma (MCF-7) and colon carcinoma (DLD-1), but not normal mammary fibroblasts.

Interaction of Human Tumor Cells with Human Platelets and the Coagulation System

1983 ◽  
Vol 50 (03) ◽  
pp. 726-730 ◽  
Author(s):  
Hamid Al-Mondhiry ◽  
Virginia McGarvey ◽  
Kim Leitzel
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Human Tumor ◽  
Human Platelets ◽  
Factor Vii ◽  
Coagulation System ◽  
Breast Adenocarcinoma ◽  
Activate Factor ◽  
Tumor Cell Lines

SummaryThis paper reports studies on the interaction between human platelets, the plasma coagulation system, and two human tumor cell lines grown in tissue culture: Melanoma and breast adenocarcinoma. The interaction was monitored through the use of 125I- labelled fibrinogen, which measures both thrombin activity generated by cell-plasma interaction and fibrin/fibrinogen binding to platelets and tumor cells. Each tumor cell line activates both the platelets and the coagulation system simultaneously resulting in the generation of thrombin or thrombin-like activity. The melanoma cells activate the coagulation system through “the extrinsic pathway” with a tissue factor-like effect on factor VII, but the breast tumor seems to activate factor X directly. Both tumor cell lines activate platelets to “make available” a platelet- derived procoagulant material necessary for the conversion of prothrombin to thrombin. The tumor-derived procoagulant activity and the platelet aggregating potential of cells do not seem to be inter-related, and they are not specific to malignant cells.

scholarly journals Ru(II)/clotrimazole/diphenylphosphine/bipyridine complexes: Interaction with DNA, BSA and biological potential against tumor cell lines and Mycobacterium tuberculosis

2016 ◽  
Vol 162 ◽  
pp. 135-145 ◽  
Author(s):  
Legna Colina-Vegas ◽  
Jocely Lucena Dutra ◽  
Wilmer Villarreal ◽  
João Honorato de A. Neto ◽  
Marcia Regina Cominetti ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tumor Cell ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Biological Potential

scholarly journals Concentration Gradient Constructions Using Inertial Microfluidics for Studying Tumor Cell–Drug Interactions

Micromachines ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 493
Author(s):  
Shaofei Shen ◽  
Fangjuan Zhang ◽  
Mengqi Gao ◽  
Yanbing Niu
Keyword(s):  
Tumor Cell ◽  
Drug Interactions ◽  
Cell Lines ◽  
Drug Concentration ◽  
Concentration Gradient ◽  
Breast Adenocarcinoma ◽  
Tumor Cell Lines ◽  
Concentration Gradients ◽  
Flow Rates ◽  
Wide Range

With the continuous development of cancer therapy, conventional animal models have exposed a series of shortcomings such as ethical issues, being time consuming and having an expensive cost. As an alternative method, microfluidic devices have shown advantages in drug screening, which can effectively shorten experimental time, reduce costs, improve efficiency, and achieve a large-scale, high-throughput and accurate analysis. However, most of these microfluidic technologies are established for narrow-range drug-concentration screening based on sensitive but limited flow rates. More simple, easy-to operate and wide-ranging concentration-gradient constructions for studying tumor cell–drug interactions in real-time have remained largely out of reach. Here, we proposed a simple and compact device that can quickly construct efficient and reliable drug-concentration gradients with a wide range of flow rates. The dynamic study of concentration-gradient formation based on successive spiral mixer regulations was investigated systematically and quantitatively. Accurate, stable, and controllable dual drug-concentration gradients were produced to evaluate simultaneously the efficacy of the anticancer drug against two tumor cell lines (human breast adenocarcinoma cells and human cervical carcinoma cells). Results showed that paclitaxel had dose-dependent effects on the two tumor cell lines under the same conditions, respectively. We expect this device to contribute to the development of microfluidic chips as a portable and economical product in terms of the potential of concentration gradient-related biochemical research.

IR spectroscopy and IR microscopy of human breast tumors, xenografted breast tumors, and breast tumor cell lines

1998 ◽  
Author(s):  
Heinz Fabian ◽  
Ralf Wessel ◽  
Michael Jackson ◽  
Arnfried Schwartz ◽  
Peter Lasch ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Tumor Cell ◽  
Cell Lines ◽  
Breast Tumor ◽  
Breast Tumors ◽  
Tumor Cell Lines ◽  
Human Breast ◽  
Breast Tumor Cell ◽  
Ir Microscopy
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