scholarly journals Diversity and Ecology of Marine Algicolous Arthrinium Species as a Source of Bioactive Natural Products

Marine Drugs ◽  
2018 ◽  
Vol 16 (12) ◽  
pp. 508 ◽  
Author(s):  
Young Mok Heo ◽  
Kyeongwon Kim ◽  
Seung Mok Ryu ◽  
Sun Lul Kwon ◽  
Min Young Park ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Large Subunit ◽  
Radical Scavenging ◽  
Elongation Factor ◽  
Bacterial Biofilm ◽  
Internal Transcribed Spacers ◽  
Translation Elongation ◽  
Bioactive Natural Products ◽  
Symbiotic Algae ◽  
Large Subunit Rdna

In our previous study, all Arthrinium isolates from Sargassum sp. showed high bioactivities, but studies on marine Arthrinium spp. are insufficient. In this study, a phylogenetic analysis of 28 Arthrinium isolates from seaweeds and egg masses of Arctoscopus japonicus was conducted using internal transcribed spacers, nuclear large subunit rDNA, β-tubulin, and translation elongation factor region sequences, and their bioactivities were investigated. They were analyzed as 15 species, and 11 of them were found to be new species. Most of the extracts exhibited radical-scavenging activity, and some showed antifungal activities, tyrosinase inhibition, and quorum sensing inhibition. It was implied that marine algicolous Arthrinium spp. support the regulation of reactive oxygen species in symbiotic algae and protect against pathogens and bacterial biofilm formation. The antioxidant from Arthrinium sp. 10 KUC21332 was separated by bioassay-guided isolation and identified to be gentisyl alcohol, and the antioxidant of Arthrinium saccharicola KUC21221 was identical. These results demonstrate that many unexploited Arthrinium species still exist in marine environments and that they are a great source of bioactive compounds.

Affinities of the Boletus chromapes group to Royoungia and the description of two new genera, Harrya and Australopilus

2012 ◽  
Vol 25 (6) ◽  
pp. 418 ◽  
Author(s):  
Roy E. Halling ◽  
Mitchell Nuhn ◽  
Todd Osmundson ◽  
Nigel Fechner ◽  
James M. Trappe ◽  
...  
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Sister Group ◽  
Molecular Data ◽  
Translation Elongation ◽  
Spore Deposit ◽  
Large Subunit Rdna ◽  
Translation Elongation Factor 1Α

Harrya is described as a new genus of Boletaceae to accommodate Boletus chromapes, a pink-capped bolete with a finely scabrous stipe adorned with pink scabers, a chrome yellow base and a reddish-brown spore deposit. Phylogenetic analyses of large-subunit rDNA and translation elongation factor 1α confirmed Harrya as a unique generic lineage with two species, one of which is newly described (H. atriceps). Some Chinese taxa were recently placed in a separate genus, Zangia, supported by both morphology and molecular data. Multiple accessions from Queensland, Australia, support the synonymy of at least three species in a separate Australian clade in the new genus, Australopilus. The truffle-like Royoungia is also supported as a separate lineage in this clade of boletes. Even though it lacks stipe characters, it possesses the deep, bright yellow to orange pigments in the peridium. Additional collections from Zambia and Thailand represent independent lineages of uncertain phylogenetic placement in the Chromapes complex, but sampling is insufficient for formal description of new species. Specimens from Java referable to Tylopilus pernanus appear to be a sister group of the Harrya lineage.

Spegazzinia camelliae sp. nov. (Didymosphaeriaceae, Pleosprales), a new endophytic fungus from northern Thailand

Phytotaxa ◽  
2021 ◽  
Vol 483 (2) ◽  
pp. 117-128
Author(s):  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
SAISAMORN LUMYONG
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Translation Elongation ◽  
Spine Length ◽  
Elongation Factor 1 Alpha

A new endophytic ascomycete, described herein as Spegazzinia camelliae, was isolated from leaves of Camellia sinensis var. assamica collected from Nan Province, Thailand. This species is characterized by basauxic conidiophores and dark brown to blackish brown α and β conidia. It can be distinguished from previously described Spegazzinia species by the spine length of the α conidia and the size of the β conidia. Multi-gene phylogenetic analyses of the small subunit (SSU), large subunit (LSU) and internal transcribed spacers (ITS) of the nuclear ribosomal DNA (rDNA) and the translation elongation factor 1-alpha (tef1) genes also support S. camelliae is a distinct new species within Spegazzinia. A full description, color photographs, illustrations and a phylogenetic tree showing the position of S. camelliae are provided.

Morphological characters and phylogenetic analysis reveal a new species of Phellinus with hooked hymenial setae from Vietnam

Phytotaxa ◽  
2018 ◽  
Vol 356 (1) ◽  
pp. 91 ◽  
Author(s):  
LIN ZHU ◽  
XING JI ◽  
JING SI ◽  
BAO-KAI CUI
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
The Status ◽  
Large Subunit Rdna ◽  
Elongation Factor 1 ◽  
A New Species

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).

Morosphaeria muthupetensis sp. nov. (Morosphaeriaceae) from India: morphological characterization and multigene phylogenetic inference

2018 ◽  
Vol 61 (4) ◽  
pp. 395-405 ◽  
Author(s):  
Bandarupalli Devadatha ◽  
Vemuri Venkateswara Sarma ◽  
Rajesh Jeewon ◽  
E.B. Gareth Jones
Keyword(s):  
Tamil Nadu ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
Morphological Characterization ◽  
Internal Transcribed Spacers ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
Mangrove Wood

Abstract A novel species of Morosphaeria, Morosphaeria muthupetensis, saprobic on intertidal mangrove wood of Rhizophora mucronata from Muthupet mangroves on Kaveri River Delta, Tamil Nadu along the East coast of India, is described in this paper. Morosphaeria muthupetensis is characterized by large sub-globose to lenticular ascomata; long cylindrical to clavate, short pedicellate asci; small, fusiform to ellipsoidal ascospores with acute ends when compared to other Morosphaeria species. A multigene phylogeny obtained from a combined nuclear large subunit, small subunit, internal transcribed spacers rDNA and translation elongation factor 1-alpha sequence analysis revealed that M. muthupetensis is phylogenetically distinct and nested in between Morosphaeria velatispora and Morosphaeria ramunculicola and all these species constitute a strongly supported monophyletic clade sister to the genus Helicascus. Detailed descriptions and illustrations of M. muthupetensis are presented and compared with other Morosphaeria species.

Alanomyces, a new genus of Aplosporellaceae based on four loci phylogeny

Phytotaxa ◽  
2017 ◽  
Vol 297 (2) ◽  
pp. 168 ◽  
Author(s):  
ROHIT SHARMA ◽  
GIRISH KULKARNI ◽  
MAHESH S. SONAWANE
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Elongation Factor ◽  
Fruit Trees ◽  
Fruit Rot ◽  
Close Relative ◽  
Natural Forests ◽  
Translation Elongation ◽  
Dna Sequence Data ◽  
Large Subunit Rdna

The Botryosphaeriales is a cosmopolitan fungal order and genera belonging to it are common opportunistic pathogens which mostly infect woody plants. They cause fruit rot, dieback, trunk rot, canker and similar diseases killing trees of natural forests, plantations and fruit trees. Relatively recently, some new families (including family Aplosporellaceae) had been established within this order delineating from family Botryosphaeriaceae. In the present study, two strains (MMI00067 and MMI00068) were isolated from soil attached to the base of a macrofungal stipe collected from the forest of Anuppur (Amarkantak), Madhya Pradesh, India. Strains were identified by DNA sequence data of four loci viz., internal transcribed spacer (ITS) of rDNA, large subunit rDNA (LSU), translation elongation factor (tef) and partial β-tubulin (βtub). Based on the phylogenetic analysis, a new fungal genus Alanomyces is proposed and is positioned within Aplosporellaceae along with Aplosporella. It is characterized by fast growing, dark greenish-black colony, long neck-like multilocular-papillate, black pycnidia with separate ostiole and small <10 µm in length, cylindrical, hyaline, guttulate spermatia. The genus Alanomyces is phylogenetically distinct from its close relative Aplosporella and proposed herein as a new monotypic genus with A. indica as type species.

scholarly journals Fungal diversity of the hypersaline Inland Sea in Qatar

2018 ◽  
Vol 61 (6) ◽  
pp. 595-609 ◽  
Author(s):  
Rashmi Fotedar ◽  
Anna Kolecka ◽  
Teun Boekhout ◽  
Jack W. Fell ◽  
Ameena Al-Malki ◽  
...  
Keyword(s):  
Water Samples ◽  
Fungal Diversity ◽  
Large Subunit ◽  
Extreme Temperature ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Large Subunit Rdna ◽  
Elongation Factor 1 ◽  
Its1 And Its2

Abstract The hypersaline Inland Sea in Qatar constitutes a unique ecosystem characterized by salinities up to saturation, extreme temperature fluctuations, and limited rainfall. To reveal the fungal diversity of this environment, we isolated fungi from water samples collected at the Inland Sea. Taxonomic identification of the isolates was done via DNA barcoding of the ITS1 and ITS2 ribosomal DNA (rDNA) domains and the D1/D2 domains of the nuclear large subunit rDNA. Additional genes, including glyceraldehyde-3-phosphate dehydrogenase (gapdh) and translation elongation factor 1-alpha (tef1), were included for isolates of Alternaria, actin (Act) for Cladosporium, part of the beta-tubulin (BenA) and calmodulin (CaM) genes for Aspergillus and Penicillium. In total, 159 fungal isolates, including 85 Ascomycota and 74 Basidiomycota, were obtained from the water samples collected during four samplings in the winter and summer seasons. About 14% (22/159) of the strains, presumably novel species, were preliminarily identified to the genus level. This is the first report highlighting the diversity of fungi from the hypersaline Inland Sea in Qatar.

Phylogenetic classification and generic delineation of Hydeomyces desertipleosporoides gen. et sp. nov., (Phaeosphaeriaceae) from Jebel Akhdar Mountain in Oman

Phytotaxa ◽  
2019 ◽  
Vol 391 (1) ◽  
pp. 28 ◽  
Author(s):  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
HIRAN A. ARIYAWANSA ◽  
DHANUSHKA N. WANASINGHE ◽  
MONIKA C. DAYARATHNE ◽  
NADIYA A. AL-SAADY ◽  
...  
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
New Taxon ◽  
Small Subunit Rrna ◽  
Translation Elongation ◽  
The Family ◽  
Large Subunit Rrna ◽  
Elongation Factor 1

Specimens of a new pleosporalean taxon were obtained on the bark of Juniperus excels from the northern mountains of Oman; from the Jebel Akhdar (‘Green Hills’). Sequence analyses based on the regions of large subunit rRNA (LSU), small subunit rRNA (SSU), translation elongation factor 1-α (TEF) and internal transcribed spacers (ITS) were performed to resolve the phylogenetic relationships of the new taxon in Phaeosphaeriaceae. The data concluded that the taxon represents a novel genus of the family Phaeosphaeriaceae and the generic name Hydeomyces and the species name H. desertipleosporoides are introduced for the new taxon. An outline of the characters which differentiate the new genus from phylogenetically closely related genera Dematiopleospora and Dlhawksworthia is given and its morphology of asexual and sexual morphs is described.

scholarly journals Multigene Phylogeny and Morphology Reveal Three Novel Species and a Novel Record of Agaricus From Northern Thailand

2021 ◽  
Vol 12 ◽  
Author(s):  
Chanyawat Jaichaliaw ◽  
Jaturong Kumla ◽  
Santhiti Vadthanarat ◽  
Nakarin Suwannarach ◽  
Saisamorn Lumyong
Keyword(s):  
Phylogenetic Trees ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Translation Elongation ◽  
Northern Thailand ◽  
Three New Species ◽  
First Time

Agaricus is a saprophytic mushroom genus widely distributed throughout the world. In this study, a survey of the Agaricus species carried out around Chiang Mai University in northern Thailand from 2018 to 2019 yielded 12 collections. Morphological characteristics and phylogenic analyses based on the internal transcribed spacers (ITS) and a fragment of the large subunit (LSU) of the nuclear ribosomal DNA (rDNA), and a fragment of the translation elongation factor 1-alpha (tef1) genes were investigated. The results revealed that these collections belong to six species including Agaricus erectosquamosus, Agaricus pallidobrunneus, Agaricus subrufescens, and three new species. Agaricus thailandensis sp. nov. was found to belong to Agaricus sect. Minores, which is placed in Agaricus subg. Minores. Aagricus pseudoerectosquamosus sp. nov. was placed in Agaricus sect. Brunneopicti within Agaricus subg. Pseudochitonia. Furthermore, Agaricus lannaensis remains an incertae sedis in Agaricus subg. Pseudochitonia. Additionally, this study was proposed that A. pallidobrunneus was discovered in Thailand for the first time. Full descriptions, color photographs, illustrations, and phylogenetic trees are provided.

Cunninghamella saisamornae (Cunninghamellaceae, Mucorales), a new soil fungus from northern Thailand

Phytotaxa ◽  
2021 ◽  
Vol 509 (3) ◽  
Author(s):  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
CHANTANA SUPO ◽  
YOICHI HONDA ◽  
TAKEHITO NAKAZAWA ◽  
...  
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Soil Fungus ◽  
Maximum Growth ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Translation Elongation ◽  
Chlamydospore Formation ◽  
Elongation Factor 1 Alpha

A new endophytic fungus, described herein as Cunninghamella saisamornae, was isolated from soil collected from Chiang Mai Province, Thailand. This species was distinguished from the previously described Cunninghamella species by the presence of a chlamydospore formation, the lack of a zygospore stage and a maximum growth temperature of 45°C. Multi-gene phylogenetic analysis of internal transcribed spacers (ITS) and large subunit (LSU) of the nuclear ribosomal DNA (rDNA), along with the translation elongation factor 1-alpha (tef-1) genes, support the finding that C. saisamornae is distinct from other species within the genus Cunninghamella. A full description, color photographs, illustrations and a phylogenetic tree showing the position of C. saisamornae are provided.

scholarly journals Fusarium incarnatum-equiseti complex from China

2019 ◽  
Vol 43 (1) ◽  
pp. 70-89 ◽  
Author(s):  
M.M. Wang ◽  
Q. Chen ◽  
Y.Z. Diao ◽  
W.J. Duan ◽  
L. Cai
Keyword(s):  
Rna Polymerase ◽  
Elongation Factor ◽  
Distribution Patterns ◽  
Morphological Characters ◽  
Ribosomal Gene ◽  
Internal Transcribed Spacers ◽  
Translation Elongation ◽  
Cultural Methods ◽  
Wide Range

The Fusarium incarnatum-equiseti species complex (FIESC) is shown to encompass 33 phylogenetic species, across a wide range of habitats/hosts around the world. Here, 77 pathogenic and endophytic FIESC strains collected from China were studied to investigate the phylogenetic relationships within FIESC, based on a polyphasic approach combining morphological characters, multi-locus phylogeny and distribution patterns. The importance of standardised cultural methods to the identification and classification of taxa in the FIESC is highlighted. Morphological features of macroconidia, including the shape, size and septum number, were considered as diagnostic characters within the FIESC. A multi-locus dataset encompassing the 5.8S nuclear ribosomal gene with the two flanking internal transcribed spacers (ITS), translation elongation factor (EF-1α), calmodulin (CAM), partial RNA polymerase largest subunit (RPB1) and partial RNA polymerase second largest subunit (RPB2), was generated to distinguish species within the FIESC. Nine novel species were identified and described. The RPB2 locus is demonstrated to be a primary barcode with high success rate in amplification, and to have the best species delimitation compared to the other four tested loci.

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