scholarly journals Anthracimycin B, a Potent Antibiotic against Gram-Positive Bacteria Isolated from Cultures of the Deep-Sea Actinomycete Streptomyces cyaneofuscatus M-169

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 406 ◽  
Author(s):  
Víctor Rodríguez ◽  
Jesús Martín ◽  
Aida Sarmiento-Vizcaíno ◽  
Mercedes de la Cruz ◽  
Luis García ◽  
...  
Keyword(s):  
Deep Sea ◽  
2D Nmr ◽  
Reversed Phase ◽  
Flash Chromatography ◽  
Preparative Hplc ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Excellent Candidate ◽  
2D Nmr Spectra ◽  
Biosynthetic Studies

The potent antimicrobial extract of a culture of the marine derived actinomycete Streptomyces cyaneofuscatus M-169 was fractionated by reversed phase flash chromatography and preparative HPLC to yield the new Gram-positive antibiotic, anthracimycin B (1), together with its congener, anthracimycin (2). The structure of the new compound was established by analysis of its ESI-TOF MS and 1D and 2D NMR spectra, and comparison with data published for anthracimycin and anthracimycin BII-2619 (3). Notably, anthracimycin seemed to be the major and almost unique component of the extract detected by HPLC-UV-MS, making our S. cyanofuscatus strain an excellent candidate for further biosynthetic studies of this potent antibiotic.

Laurentixanthone C: A New Antifungal and Algicidal Xanthone from Stem Bark of Vismia laurentii

2007 ◽  
Vol 62 (4) ◽  
pp. 565-568 ◽  
Author(s):  
Michel F. Tala ◽  
Karsten Krohn ◽  
Hidayat Hussain ◽  
Simeon F. Kouamc ◽  
Hippolyte K. Wabo ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Nmr Spectra ◽  
2D Nmr ◽  
Stem Bark ◽  
Spectroscopic Studies ◽  
Chlorella Fusca ◽  
Gram Positive ◽  
Preliminary Results ◽  
Gram Positive Bacteria ◽  
2D Nmr Spectra

Laurentixanthone C (1), a new xanthone, was isolated from the stem bark of Vismia laurentii (Guttiferae or Clusiaceae), in addition to the known compounds vismiaquinone (2), bisvismiaquinone (3), and dammaradienol (4). The structures were established based on spectroscopic studies, notably of the 2D NMR spectra. Preliminary results showed that 1 is algicidal and strongly antibacterial against the Gram-positive bacteria Bacillus megaterium and Chlorella fusca, respectively.

A Cytotoxic Natural Product from Patrinia villosa Juss

2019 ◽  
Vol 19 (11) ◽  
pp. 1399-1404 ◽  
Author(s):  
Yangcheng Liu ◽  
Wei Liu ◽  
Changlan Chen ◽  
Zheng Xiang ◽  
Hongwei Liu
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Silica Gel ◽  
Column Chromatography ◽  
Bioactive Compound ◽  
Antitumor Agent ◽  
2D Nmr ◽  
Preparative Hplc ◽  
Dependent Manner ◽  
2D Nmr Spectra

Background and Purpose:: Patrinia villosa Juss is an important Chinese herbal medicine widely used for thousands of years, but few reports on the ingredients of the herb have been presented. In this study, we aim to isolate the bioactive compound from the plant. Material and Methods:: The air-dried leaves of P. villosa (15kg) were extracted three times with 70% EtOH under reflux. The condensed extract was suspended in H2O and partitioned with light petroleum, dichloromethane and n-BuOH. The dichloromethane portion was then subjected to normal-phase silica gel column chromatography, ODS silica gel column chromatography and semi-preparative HPLC to yield compound 1. Cytotoxicities of 1 were assayed on HepG2, A549 and A2780 cell lines. The mechanism of apoptosis and cell cycle on A549 was confirmed subsequently. Results: A new impecylone (Impecylone A) was isolated from the leaves of Patrinia villosa Juss, and its structures were established using 1D, 2D-NMR spectra and HR-ESI-MS. Impecylone A could selectivity inhibit HepG2 and A549 cell lines. The compound could induce apoptosis of A549 and arrest the cell cycle at G2/M phase in a dose-dependent manner. Conclusion: Impecylone A is a novel compound from Patrinia villosa Juss and could be a potential antitumor agent especially in the cell lines of A549.

scholarly journals Isolation and Structural Characterization of Degradation Products of Aceclofenac by HPLC, HRMS and 2D NMR

2019 ◽  
Vol 31 (4) ◽  
pp. 851-854
Author(s):  
Santhosh Guduru ◽  
V.V.S.R.N. Anji Karun Mutha ◽  
B. Vijayabhaskar ◽  
Muralidharan Kaliyaperumal ◽  
Raghu Babu Korupolu ◽  
...  
Keyword(s):  
Degradation Product ◽  
Degradation Products ◽  
Acid Stress ◽  
2D Nmr ◽  
Stress Conditions ◽  
Preparative Hplc ◽  
Mass Spectral ◽  
2D Nmr Spectra ◽  
The Stability

The stability of aceclofenac under stress conditions was assessed to identify the degradation products. So, it was subjected to stress conditions like acid, base and oxidation, according to ICH guideline Q1A (R2). One degradation product formed when the drug was subjected to acid stress. Three degradation products were formed during the basic stress condition. The drug substance was found to be stable to oxidative stress. The degradants formed during the stress were separated on a C-18 column using gradient preparative HPLC elution. The only product (DP-2) formed during the acid stress and this one is same as of one of the three degradation products (DP-1, DP-2, DP-3) were formed during base stress. 1D and 2D NMR spectra and mass spectral analysis supported the proposed structures for the products. The products DP-2 and DP-3 have been reported earlier but this is the first report of product DP-1 as a degradation product of aceclofenac.

scholarly journals Advances in the Dereplication of Aroma Precursors from Grape Juice by Pretreatment with Lead Acetate and Combined HILIC- and RP-HPLC Methods

Foods ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 28
Author(s):  
Michele D’Ambrosio
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Grape Juice ◽  
2D Nmr ◽  
Reversed Phase ◽  
Natural Form ◽  
Chemical Structures ◽  
Rp Hplc ◽  
2D Nmr Spectra ◽  
Aroma Precursors

Glycosidic aroma precursors (GAPs) contribute to the varietal flavor of wine. Researchers have applied various sample preparation and analytical methods in attempts to achieve their separation and identification. However, mass spectrometric methods still fail to unequivocally define their structures. We have previously reported the separation of GAPs in their natural form and elucidated their structures by nuclear magnetic resonance (NMR) spectroscopy. In this study, we confirm the effectiveness of our established procedure and present methodological improvements. Grape juice was treated with lead (II) acetate and repeatedly chromatographed to give seven pure GAPs. Their chemical structures were characterized by MSn fragmentations and 1D- and 2D-NMR spectra. Ten GAPs were analyzed by both hydrophilic interaction liquid chromatography (HILIC) and reversed phase high performance liquid chromatography (RP-HPLC) to compare the two chromatograms. A selection of known phenols was treated with lead (II) acetate in order to check its binding properties.

scholarly journals Crystallomycin revisited after 60 years: aspartocins B and C

MedChemComm ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 667-675 ◽  
Author(s):  
Anton P. Tyurin ◽  
Vera A. Alferova ◽  
Alexander S. Paramonov ◽  
Maxim V. Shuvalov ◽  
Irina A. Malanicheva ◽  
...  
Keyword(s):  
2D Nmr ◽  
Dmso Solution ◽  
Gram Positive ◽  
Gram Positive Bacteria

Archived sample contains Ca2+-dependent lipopeptides active against Gram-positive bacteria; conformers of aspartocin B in DMSO solution were studied by 2D NMR.

scholarly journals A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4383
Author(s):  
Weihong Wang ◽  
Kyu-Hyung Park ◽  
Jusung Lee ◽  
Eunseok Oh ◽  
Chanyoon Park ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution Mass Spectrometry ◽  
2D Nmr ◽  
Antibacterial Activities ◽  
Culture Broth ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
New Antibiotics ◽  
Thiopeptide Antibiotics ◽  
Bacterium Bacillus

A new thiopeptide (micrococcin P3, 1) and a known one (micrococcin P1, 2) were isolated from the culture broth of a marine-derived strain of Bacillus stratosphericus. The structures of both compounds were elucidated using spectroscopic methods, including extensive 1D and 2D NMR analysis, high resolution mass spectrometry (HRMS), and tandem mass spectrometry. Both compounds exhibited potent antibacterial activities against Gram-positive strains with minimum inhibitory concentration (MIC) values of 0.05−0.8 μg/mL and did not show cytotoxicity in the MTT assay up to a concentration of 10 μM. This study adds a new promising member, micrococcin P3, to the family of thiopeptide antibiotics, which shows potential for the development of new antibiotics targeting Gram-positive bacteria.

Phylogenetic diversity of Gram-positive bacteria cultured from Antarctic deep-sea sponges

Polar Biology ◽  
2011 ◽  
Vol 34 (10) ◽  
pp. 1501-1512 ◽  
Author(s):  
Yanjuan Xin ◽  
Manmadhan Kanagasabhapathy ◽  
Dorte Janussen ◽  
Song Xue ◽  
Wei Zhang
Keyword(s):  
Deep Sea ◽  
Phylogenetic Diversity ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals The Macrocyclic Peptide Antibiotic Micrococcin P1 Is Secreted by the Food-Borne Bacterium Staphylococcus equorumWS 2733 and Inhibits Listeria monocytogenes on Soft Cheese

2000 ◽  
Vol 66 (6) ◽  
pp. 2378-2384 ◽  
Author(s):  
Markus C. Carnio ◽  
Alexandra Höltzel ◽  
Melanie Rudolf ◽  
Thomas Henle ◽  
Günther Jung ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Starter Culture ◽  
Reversed Phase ◽  
Resonance Spectroscopy ◽  
Peptide Antibiotic ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Cheese Ripening ◽  
Staphylococcus Equorum ◽  
Macrocyclic Peptide

ABSTRACT Staphylococcus equorum WS 2733 was found to produce a substance exhibiting a bacteriostatic effect on a variety of gram-positive bacteria. The metabolite was purified to homogeneity by ammonium sulfate precipitation and semipreparative reversed-phase high-performance liquid chromatography. Electrospray mass spectrometry confirmed the high purity of the compound and revealed a molecular mass of 1,143 Da. By two-dimensional nuclear magnetic resonance spectroscopy the substance was identified as micrococcin P1 which is a macrocyclic peptide antibiotic that has not yet been reported for the genus Staphylococcus. A total of 95 out of 95Listeria strains and 130 out of 135 other gram-positive bacteria were inhibited by this substance, while none of 37 gram-negative bacteria were affected. The antilisterial potential of this food-grade strain as a protective starter culture was evaluated by its in situ application in cheese-ripening experiments under laboratory conditions. A remarkable growth reduction of Listeria monocytogenes could be achieved compared to control cheese ripened with a nonbacteriocinogenic type strain of Staphylococcus equorum. In order to prove that inhibition was due to micrococcin P1, a micrococcin-deficient mutant was constructed which did not inhibit L. monocytogenes in cheese-ripening experiments.

Influence of Cell Wall Composition on the Fossilisation of Bacteria and the Implications for the Search for Early Life Forms

1997 ◽  
Vol 161 ◽  
pp. 491-504 ◽  
Author(s):  
Frances Westall
Keyword(s):  
Cell Wall ◽  
Life Forms ◽  
Cation Binding ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Hydrothermal Sediments ◽  
Identification Of Bacteria ◽  
The Difference

AbstractThe oldest cell-like structures on Earth are preserved in silicified lagoonal, shallow sea or hydrothermal sediments, such as some Archean formations in Western Australia and South Africa. Previous studies concentrated on the search for organic fossils in Archean rocks. Observations of silicified bacteria (as silica minerals) are scarce for both the Precambrian and the Phanerozoic, but reports of mineral bacteria finds, in general, are increasing. The problems associated with the identification of authentic fossil bacteria and, if possible, closer identification of bacteria type can, in part, be overcome by experimental fossilisation studies. These have shown that not all bacteria fossilise in the same way and, indeed, some seem to be very resistent to fossilisation. This paper deals with a transmission electron microscope investigation of the silicification of four species of bacteria commonly found in the environment. The Gram positiveBacillus laterosporusand its spore produced a robust, durable crust upon silicification, whereas the Gram negativePseudomonas fluorescens, Ps. vesicularis, andPs. acidovoranspresented delicately preserved walls. The greater amount of peptidoglycan, containing abundant metal cation binding sites, in the cell wall of the Gram positive bacterium, probably accounts for the difference in the mode of fossilisation. The Gram positive bacteria are, therefore, probably most likely to be preserved in the terrestrial and extraterrestrial rock record.

Microanatomy of the Periplasm of Bacillus Licheniformis

1971 ◽  
Vol 29 ◽  
pp. 262-263
Author(s):  
B.K. Ghosh
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Bacillus Licheniformis ◽  
External Environment ◽  
Permeability Barrier ◽  
Periplasmic Space ◽  
Modified Technique ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Periplasm of bacteria is the space outside the permeability barrier of plasma membrane but enclosed by the cell wall. The contents of this special milieu exterior could be regulated by the plasma membrane from the internal, and by the cell wall from the external environment of the cell. Unlike the gram-negative organism, the presence of this space in gram-positive bacteria is still controversial because it cannot be clearly demonstrated. We have shown the importance of some periplasmic bodies in the secretion of penicillinase from Bacillus licheniformis.In negatively stained specimens prepared by a modified technique (Figs. 1 and 2), periplasmic space (PS) contained two kinds of structures: (i) fibrils (F, 100 Å) running perpendicular to the cell wall from the protoplast and (ii) an array of vesicles of various sizes (V), which seem to have evaginated from the protoplast.

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