scholarly journals Two Novel Aspochalasins from the Gut Fungus Aspergillus sp. Z4

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 343 ◽  
Author(s):  
Xinyang Li ◽  
Wanjing Ding ◽  
Pinmei Wang ◽  
Jinzhong Xu
Keyword(s):  
Prostate Cancer ◽  
Nuclear Magnetic Resonance ◽  
Culture Broth ◽  
Strong Activity ◽  
Mtt Method ◽  
Weak Activity ◽  
Pc3 Cell Line ◽  
Aspergillus Sp ◽  
Modified Mosher’S Method ◽  
Ionisation Mass

Two novel aspochalasins, tricochalasin A (1) and aspochalasin A2 (2), along with three known compounds (3–5) have been isolated from the different culture broth of Aspergillus sp., which was found in the gut of a marine isopod Ligia oceanica. Compound 1 contains a rare 5/6/6 tricyclic ring fused with the aspochalasin skeleton. The structures were determined on the basis of electrospray ionisation mass spectroscopy (ESIMS), nuclear magnetic resonance (NMR) spectral data, and the absolute configurations were further confirmed by modified Mosher’s method. Cytotoxicity against the prostate cancer PC3 cell line were assayed by the MTT method. Compound 3 showed strong activity while the remaining compounds showed weak activity.

scholarly journals Asperginine, an Unprecedented Alkaloid from the Marine-derived Fungus Aspergillus sp

2015 ◽  
Vol 10 (8) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Pinmei Wang ◽  
Shizhe Zhao ◽  
Ying Liu ◽  
Wanjing Ding ◽  
Feng Qiu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Spectral Data ◽  
Absolute Configuration ◽  
Marine Fungus ◽  
Mass Spectral Data ◽  
Hydrogen Atoms ◽  
Relative Configuration ◽  
Mass Spectral ◽  
Mtt Method ◽  
Aspergillus Sp

Asperginine (1), an alkaloid possessing a rare skeleton, was isolated from the cultural broth of the marine fungus Aspergillus sp. (Z-4) isolated from the gut of the marine isopod Ligia oceanica. The planar structure and relative configuration of 1 was determined by analysis of NMR and mass spectral data. Its absolute configuration was elucidated by Marfey's method, together with NOESY correlations of key hydrogen atoms. The cytotoxicity against prostate cancer PC3 and human HCT116 was assayed by the MTT method. Unfortunately, asperginine did not show any activity.

scholarly journals Chemical Constituents From the Vietnamese Medicinal Plant Anemone chapaensis and Their Cytotoxic Activity

2019 ◽  
Vol 14 (8) ◽  
pp. 1934578X1987099
Author(s):  
Ha Thi Thanh Huong ◽  
Pham G. Nam ◽  
Hoang Van Hung ◽  
Phi T. Xuyen ◽  
Nguyen M. Khoi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Medicinal Plant ◽  
Chemical Constituents ◽  
Cytotoxic Activities ◽  
Spectroscopic Methods ◽  
Strong Activity ◽  
Mtt Method ◽  
Phytochemical Constituents ◽  
Phytochemical Profile

Our current research on phytochemical profile of the folk medicinal plant Anemone chapaensis Gagnep. led to the isolation of a new saponin, chapaenoside (1), along with 13 known compounds (2-14). Their structures were elucidated on the basis of extensive chemical and spectroscopic methods (Nuclear Magnetic Resonance [NMR] spectroscopy and mass spectrometry) as well as comparison with the literature data. The isolated compounds were tested for their cytotoxic activities against 9 cancer cell lines by MTT method. The result indicated that the major saponin prosapogenin CP6 (5) showed selectively strong activity against NCI-N87, RD, Panc-1, and MIA Paca-2 cells with the IC50 values of 5.4, 7.5, 7.5, and 2.7 μM, respectively. The results of phytochemical constituents and principal bioactive saponins from A. chapaensis contributed not only to its phytochemical profile associated with chemotaxonomy but also to biological evidence of the title plant.

scholarly journals Anti-dormant Mycobacterial Activity of Viomellein and Xanthomegnin, Naphthoquinone Dimers Produced by Marine-derived Aspergillus sp

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200
Author(s):  
Kentaro Kamiya ◽  
Masayoshi Arai ◽  
Andi Setiawan ◽  
Motomasa Kobayashi
Keyword(s):  
Mycobacterium Bovis ◽  
Plasmid Dna ◽  
Active Fraction ◽  
Mycobacterium Bovis Bcg ◽  
Mycobacterial Genome ◽  
Weak Activity ◽  
Aspergillus Sp ◽  
Chelating Effect

In the course of a search for anti-dormant mycobacterial substances from marine-derived microorganisms, viomellein (1) and xanthomegnin (2) were rediscovered from the active fraction of the culture of a marine-derived Aspergillus sp. together with rubrosulphin (3) and asteltoxin (4) on the guidance of bioassay-guided separation. In particular, compound 1 showed higher activity against the dormant than against actively growing Mycobacterium bovis BCG and weak activity against M. smegmatis. Furthermore, evidence that compound 1 did not directly bind to plasmid DNA suggests its anti-mycobacterial activity differs from its direct chelating effect on the mycobacterial genome.

scholarly journals Aspergiloid I, an unprecedented spirolactone norditerpenoid from the plant-derived endophytic fungus Aspergillus sp. YXf3

2014 ◽  
Vol 10 ◽  
pp. 2677-2682 ◽  
Author(s):  
Zhi Kai Guo ◽  
Rong Wang ◽  
Wei Huang ◽  
Xiao Nian Li ◽  
Rong Jiang ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Biosynthetic Pathway ◽  
Culture Broth ◽  
Ring System ◽  
X Ray Diffraction ◽  
X Ray ◽  
Anti Oxidant ◽  
Inhibitory Activities ◽  
Aspergillus Sp

An unusual C18 norditerpenoid, aspergiloid I (1), was isolated from the culture broth of Aspergillus sp. YXf3, an endophytic fungus derived from Ginkgo biloba. Its structure was unambiguously established by analysis of HRMS–ESI and spectroscopic data, and the absolute configuration was determined by low-temperature (100 K) single crystal X-ray diffraction with Cu Kα radiation. This compound is structurally characterized by a new carbon skeleton with an unprecedented 6/5/6 tricyclic ring system bearing an α,β-unsaturated spirolactone moiety in ring B, and represents a new subclass of norditerpenoid, the skeleton of which is named aspergilane. The hypothetical biosynthetic pathway for 1 was also proposed. The cytotoxic, antimicrobial, anti-oxidant and enzyme inhibitory activities of 1 were evaluated.

Seasonal variations in enzyme histochemistry of accessory sex glands in buffalo bull

2018 ◽  
Author(s):  
Samikshya Sarangi ◽  
Anuradha Gupta ◽  
Neelam Bansal ◽  
Varinder Uppal
Keyword(s):  
Enzyme Histochemistry ◽  
Physiological Activity ◽  
Winter Season ◽  
Acinar Cells ◽  
Summer Season ◽  
Moderate Activity ◽  
Strong Activity ◽  
Accessory Sex Glands ◽  
Weak Activity ◽  
Cryostat Sections

The present study was conducted on vesicular, prostate and bulbourethral glands of buffalo bullcollected from the abattoir immediately after sacrifice during winter and summer season. The cryostat sections of 10-12 µm thickness were obtained at -23°C and were incubated in different substrates for demonstration of phosphatases, oxidoreductases and estrases. During winter season, the moderate to strong activity of AKPase in acinar cells and ductular epithelium was observed in vesicular, weak in prostate, but the activity was not demonstrated in bulbourethral gland, however the blood vessels showed moderate AKPase reaction in all the glands. A moderate activity of SDH, LDH and G-6-PD was observed in glandular and ductular epithelium of vesicular gland and weak to moderate activity in prostate and bulbourethral glands. The glandular and the ductular epithelium of all the glands studied were strongly positive for NADH and NADPH, whereas a weak activity for NSE was observed. There was decrease in the activity of all the enzymes during summer season which may be related to decrease physiological activity in this season.

Comparison of glycolytic, pentose phosphate pathway, glyoxylate shunt, Krebs' cycle enzymes in Ganeo tigrinum parasitizing hibernating and non-hibernating Rana cyanophlyctis and R. tigrina

1983 ◽  
Vol 57 (1) ◽  
pp. 59-68 ◽  
Author(s):  
P. N. Sharma ◽  
Sushila Mandawat
Keyword(s):  
Isocitrate Lyase ◽  
Krebs Cycle ◽  
Pentose Phosphate ◽  
Glyoxylate Shunt ◽  
Strong Activity ◽  
Key Enzymes ◽  
Phosphogluconate Dehydrogenase ◽  
Weak Activity ◽  
Krebs Cycle Enzymes ◽  
Glucose 6 Phosphate Dehydrogenase

AbstractThe histochemical site and distribution of hexokinase, glycogen phosphorylase (GP Rylase), lactate dehydrogenase (LDH) (key enzymes of glycolysis), glucose-6-phosphate dehydrogenase (GPD) and 6-phosphogluconate dehydrogenase (6PGD) (pentose phosphate shunt enzymes), isocitrate dehydrogenase (IDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), and α-ketoglutarate dehydrogenase (α-KDH) (key enzymes of Krebs' cycle), malate synthetase (MS) and isocitrate lyase (IL) (enzymes of glyoxylate shunt) in various tissues of Ganeo tigrinum from hibernating and non-hibernating Rana cyanophlyctis and R. tigrina were studied. Differences in their intensities were revealed. Weak activity of GP Rylase and strong activity of hexokinase in flukes from non-hibernating hosts indicates that they utilize glucose through glycolysis for energy turnover. Intense GP Rylase and weak hexokinase activity in worms from hibernating hosts indicates the utilization of glycogen. Strong activity of IDH, SDH, MDH, α-KGD, MS and IL was demonstrable in the tissues of flukes from non-hibernating hosts, suggesting that Krebs' cycle and glyoxylate shunt, respectively, were operating. Tissues of the fluke from hibernating hosts, on the other hand, displayed positive activity only for SDH and MDH; no activity for MS and IL, the enzymes of glyoxylate shunt, was observed, The activity of the above enzymes was found to be relatively low in worms from hibernating hosts.

scholarly journals A New Macrolide from a Marine-derived Fungus Aspergillus sp

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Jie Bao ◽  
Xin-Ya Xu ◽  
Xiao-Yong Zhang ◽  
Shu-Hua Qi
Keyword(s):  
Influenza Virus ◽  
Mass Spectra ◽  
Inhibitory Effect ◽  
Culture Broth ◽  
Aspergillus Sp ◽  
Virus Strains

A new 16-membered macrolide named aspergillide D (1), along with six known compounds, including two polyketones (2–3) and four alkaloids (4–7), were isolated from the culture broth of a marine-derived fungus Aspergillus sp. SCSGAF 0076. The structure of 1 was elucidated on the basis of NMR and mass spectra. Compound 5 showed an obvious inhibitory effect on influenza virus strains H1N1 and H3N2.

scholarly journals Antimicrobial Lavandulylated Flavonoids from a Sponge-Derived Streptomyces sp. G248 in East Vietnam Sea

Marine Drugs ◽  
2019 ◽  
Vol 17 (9) ◽  
pp. 529 ◽  
Author(s):  
Cao ◽  
Trinh ◽  
Mai ◽  
Vu ◽  
Le ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Antimicrobial Activity ◽  
Mycobacterium Tuberculosis ◽  
Spectroscopic Data ◽  
Inhibitory Concentration ◽  
Culture Broth ◽  
Ionization Mass ◽  
Streptomyces Sp ◽  
Mycobacterium Tuberculosis H37rv

Three new lavandulylated flavonoids, (2S,2′’S)-6-lavandulyl-7,4′-dimethoxy-5,2′-dihydroxylflavanone (1), (2S,2′’S)-6-lavandulyl-5,7,2′,4′-tetrahydroxylflavanone (2), and (2′’S)-5′-lavandulyl-2′-methoxy-2,4,4′,6′-tetrahydroxylchalcone (3), along with seven known compounds 4–10 were isolated from culture broth of Streptomyces sp. G248. Their structures were established by spectroscopic data analysis, including 1D and 2D nuclear magnetic resonance (NMR), and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). The absolute configurations of 1–3 were resolved by comparison of their experimental and calculated electronic circular dichroism spectra. Compounds 1–3 exhibited remarkable antimicrobial activity. Whereas, two known compounds 4 and 5 exhibited inhibitory activity against Mycobacterium tuberculosis H37Rv with minimum inhibitory concentration (MIC) values of 6.0 µg/mL and 11.1 µg/mL, respectively.

scholarly journals Discovery of Nosiheptide, Griseoviridin, and Etamycin as Potent Anti-Mycobacterial Agents against Mycobacterium avium Complex

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1495
Author(s):  
Hosoda ◽  
Koyama ◽  
Kanamoto ◽  
Tomoda
Keyword(s):  
Therapeutic Effect ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Complex ◽  
Culture Broth ◽  
Therapeutic Effects ◽  
Strong Activity ◽  
Minimum Inhibitory Concentrations ◽  
Marine Actinomycete ◽  
Serious Disease ◽  
Microbial Samples

Mycobacterium avium complex (MAC) is a serious disease mainly caused by M. avium and M. intracellulare. Although the incidence of MAC infection is increasing worldwide, only a few agents are clinically used, and their therapeutic effects are limited. Therefore, new anti-MAC agents are needed. Approximately 6600 microbial samples were screened for new anti-mycobacterial agents that inhibit the growth of both M. avium and M. intracellulare, and two culture broths derived from marine actinomycete strains OPMA1245 and OPMA1730 had strong activity. Nosiheptide (1) was isolated from the culture broth of OPMA1245, and griseoviridin (2) and etamycin (viridogrisein) (3) were isolated from the culture broth of OPMA1730. They had potent anti-mycobacterial activity against M. avium and M. intracellulare with minimum inhibitory concentrations (MICs) between 0.024 and 1.56 μg/mL. In addition, a combination of 2 and 3 markedly enhanced the anti-mycobacterial activity against both M. avium and M. intracellulare. Furthermore, a combination 2 and 3 had a therapeutic effect comparable to that of ethambutol in a silkworm infection assay with M. smegmatis.

Sign in / Sign up

Export Citation Format

Share Document