scholarly journals Green Synthesis of Gold Nanoparticles Using Carrageenan Oligosaccharide and Their In Vitro Antitumor Activity

Marine Drugs ◽  
2018 ◽  
Vol 16 (8) ◽  
pp. 277 ◽  
Author(s):  
Xiangyan Chen ◽  
Xia Zhao ◽  
Yanyun Gao ◽  
Jiaqi Yin ◽  
Mingyue Bai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Localized Surface Plasmon ◽  
Cytotoxic Activities ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Hct 116 ◽  
Means Of Transmission

Gold nanoparticles (AuNPs) have been widely used in catalysis, photothermal therapy, and targeted drug delivery. Carrageenan oligosaccharide (CAO) derived from marine red algae was used as a reducing and capping agent to obtain AuNPs by an eco-friendly, efficient, and simple synthetic route for the first time. The synthetic conditions of AuNPs were optimized by response surface methodology (RSM), and the CAO-AuNPs obtained were demonstrated to be ellipsoidal, stable and crystalline by means of transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-ray diffraction (XRD). The CAO-AuNPs showed localized surface plasmon resonance (LSPR) oscillation at about 530 nm with a mean diameter of 35 ± 8 nm. The zeta potential of CAO-AuNPs was around −20 mV, which was related to the negatively charged CAO around AuNPs. The CAO-AuNPs exhibited significant cytotoxic activities to HCT-116 and MDA-MB-231 cells, which could be a promising nanomaterial for drug delivery.

scholarly journals Bioactive Polysaccharides Based Graphene Oxide Nanoparticle as a Promising Carrier for Anticancer Drug Delivery

2021 ◽  
Vol 12 (3) ◽  
pp. 3429-3445
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery ◽  
Graphene Oxide ◽  
X Ray Diffraction ◽  
Hemolysis Assay ◽  
Anticancer Drug Delivery ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Electron Microscopy Analysis

Nowadays, the concept of drug transmission is a prominent issue in the world of drug delivery research. We investigated the development of a hybrid platform based on graphene oxide/chitosan and xyloglucan (GO-CH-Xn) for the loading and release of doxorubicin (DOX)., where chitosan (CS) natural polymer functionalizes graphene oxide and is then grafted by xyloglucan (Xn) natural hydrophilic polysaccharide to form a reliable nanocarrier system for the delivery of DOX. UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Raman spectroscopy, transmission electron microscopy, and scanning electron microscopy analysis were used to confirm the fundamental physicochemical properties. The DOX loading capacity and efficiency were 81.8% and 73.5%. The graphene oxide-chitosan-xyloglucan- doxorubicin (GO-CS-Xn-DOX) drug delivery system showed a pH-regulated release as observed by UV analysis. Biocompatibility was evaluated via in vitro hemolysis assay, indicates negligible toxicity, and the anticancer activity of the developed nanocarrier system was studied by 3-(4, 5-dimethylthiazol-2-Y)-2,5-diphenyltetrazolium bromide (MTT) against human (U 87) glioblastoma cancer cell lines. The in vitro studies demonstrate the major advantages of the developed approach by demonstrating its capability as a promising nanocarrier for biomedical applications.

Synthesis and Study the Properties of a Solar Cell of Gold Nanoparticles Prepared in a Simple Chemical Way

2021 ◽  
Vol 19 (11) ◽  
pp. 66-71
Author(s):  
Maithm A. Obaid ◽  
Suha A Fadaam ◽  
Osama S. Hashim
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Chemical Method ◽  
X Ray Diffraction ◽  
Structural And Optical Properties ◽  
Casting Method ◽  
X Ray ◽  
Transmission Electron ◽  
Simple Chemical ◽  
Scanning Electron

The aim of this study is to prepare gold nanoparticles by a simple chemical method at a temperature of 70°C. The solution was dried on glass basest by Casting method, the rate of five drops per sample At a temperature 100 C. Then the structural and optical properties have been confirmed by X-ray diffraction, scanning electron microscopy (SEM) and Transmission Electron microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and spectrum.

scholarly journals Biosynthesized gold nanoparticles-doped hydroxyapatite as antibacterial and antioxidant nanocomposite

2021 ◽  
Author(s):  
Is Fatimah ◽  
Putwi Widya Citradewi ◽  
Amri Yahya ◽  
Bambang Nugroho ◽  
Habibi Hidayat ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antioxidant Activity ◽  
Particle Size ◽  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract The composite of green synthesized gold nanoparticles (Au NPs)-doped hydroxyapatite (HA) has been prepared. The gold nanoparticles were produced via bioreduction of HAuCl4 with Clitoria ternatea flower extract, and utilized in the synthesis of hydroxyapatite using Ca(OH)2 and ammonium diphosphate as precursor. The aim of this research is to study the structural analysis of the composite and antibacterial activity test toward Eschericia coli, Staphylococcus aureus, Klebsiela pneumoniae, and Streptococcus pyogenes. In addition, the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The monitoring of gold nanoparticles formation was conducted by UV–vis spectroscopy and particle size analyses, meanwhile the synthesized composite was studied using X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The results showed that homogeneously dispersed gold nanoparticles in HA structure was obtained with the particle size ranging at 5-80 nm. The nanocomposite demonstrated antibacterial activity against tested bacteria. The nanocomposite expressed an antioxidant activity as shown by the DPPH scavenging activity of 66 and 58% at the concentration of 100 μg/mL and 50 μg/mL, respectively.

Preparation of Cellulose Acetate Ultra-Fine Fibers Containing Quasi-Spherical Gold Nanoparticles by Electrospinning Method

2011 ◽  
Vol 391-392 ◽  
pp. 400-403
Author(s):  
Dong Mei Zhao ◽  
Li Guo Sun ◽  
Li Li Lv ◽  
Jian Li
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Cellulose Acetate ◽  
Trisodium Citrate ◽  
X Ray Diffraction ◽  
Au Nps ◽  
Transmission Electron ◽  
Electrospinning Method ◽  
Before And After ◽  
Spherical Gold Nanoparticles

Quasi-spherical gold nanoparticles(Au NPs) prepared by trisodium citrate reduction of HAuCl4were dispersed into cellulose acetate(CA) ultra-fine fibers by electrospinning. The optical properties of Au NPs before and after electrospinning were measured by UV-vis spectrometer. The morphology and distribution of Au NPs in CA ultra-fine fibers were observed by transmission electron microscopy (TEM). The morphology and diameter of Au NPs/CA fibers were studied by scanning electron microscopy (SEM). The crystallinity change of CA fiber before and after adding Au NPs was characterized by X-ray diffraction (XRD).

Self-Assembly of Gold Nanoparticles on Gold Core-Induced Polypyrrole Nanohybrids for Electrochemical Sensor of Dopamine

NANO ◽  
2015 ◽  
Vol 10 (08) ◽  
pp. 1550115 ◽  
Author(s):  
Junwei Ding ◽  
Kai Zhang ◽  
Wei Xu ◽  
Zhiqiang Su
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Self Assembly ◽  
Chemical Oxidation ◽  
X Ray Diffraction ◽  
Detection Range ◽  
In Situ Chemical Oxidation ◽  
Gold Core ◽  
Transmission Electron ◽  
Chemical Oxidation Polymerization

Gold core-induced polypyrrole nanohybrids (Au–PPyNHs) were successfully synthesized via in situ chemical oxidation polymerization of pyrrole molecules, and their structure was directly confirmed and characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. Furthermore, gold nanoparticles (AuNPs) were assembled onto the as-prepared Au–PPyNHs by electrostatic interaction to fabricate the nanohybrids of Au–PPyNH–Au. The created Au–PPyNH–Au nanohybrids was immobilized onto glassy carbon electrode and applied to construct dopamine (DA) sensor. We found that the fabricated sensor with Au–PPyNH–Au nanohybrids is highly specific probe for sensing DA. The Au–PPyNH–Au based DA sensor has a linear detection range from 1[Formula: see text][Formula: see text]M to 0.321 mM and a detection limit of 0.32[Formula: see text][Formula: see text]M.

scholarly journals Fluorescence and electron microscopy to visualize the intracellular fate of nanoparticles for drug delivery

2016 ◽  
Vol 60 (2) ◽  
Author(s):  
M. Costanzo ◽  
F. Carton ◽  
A. Marengo ◽  
G. Berlier ◽  
B. Stella ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery ◽  
Transmission Electron Microscopy ◽  
Cultured Cells ◽  
Cell System ◽  
Functional Relationships ◽  
Cell Internalization ◽  
Transmission Electron ◽  
Intracellular Fate

<p>In order to design valid protocols for drug release <em>via</em> nanocarriers, it is essential to know the mechanisms of cell internalization, the interactions with organelles, and the intracellular permanence and degradation of nanoparticles (NPs) as well as the possible cell alteration or damage induced. In the present study, the intracellular fate of liposomes, polymeric NPs and mesoporous silica NPs (MSN) has been investigated in an <em>in vitro</em> cell system by fluorescence and transmission electron microscopy. The tested nanocarriers proved to be characterized by specific interactions with the cell: liposomes enter the cells probably by fusion with the plasma membrane and undergo rapid cytoplasmic degradation; polymeric NPs are internalized by endocytosis, occur in the cytoplasm both enclosed in endosomes and free in the cytosol, and then undergo massive degradation by lysosome action; MSN are internalized by both endocytosis and phagocytosis, and persist in the cytoplasm enclosed in vacuoles. No one of the tested nanocarriers was found to enter the nucleus. The exposure to the different nanocarriers did not increase cell death; only liposomes induced a reduction of cell population after long incubation times, probably due to cell overloading. No subcellular damage was observed to be induced by polymeric NPs and MSN, whereas transmission electron microscopy revealed cytoplasm alterations in liposome-treated cells. This important information on the structural and functional relationships between nanocarriers designed for drug delivery and cultured cells further proves the crucial role of microscopy techniques in nanotechnology.</p>

scholarly journals Synthesis and Characterization of a Novel Biocompatible Alloy, Ti-Nb-Zr-Ta-Sn

2021 ◽  
Vol 22 (19) ◽  
pp. 10611
Author(s):  
Yuliya Y. Khrunyk ◽  
Sabrina Ehnert ◽  
Stella V. Grib ◽  
Anatoly G. Illarionov ◽  
Stepan I. Stepanov ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Elastic Modulus ◽  
Inflammatory Responses ◽  
X Ray Diffraction ◽  
In Vitro Biocompatibility ◽  
Transmission Electron ◽  
Low Elastic Modulus ◽  
O2 Plasma Treatment ◽  
Primary Human Osteoblasts

Many current-generation biomedical implants are fabricated from the Ti-6Al-4V alloy because it has many attractive properties, such as low density and biocompatibility. However, the elastic modulus of this alloy is much larger than that of the surrounding bone, leading to bone resorption and, eventually, implant failure. In the present study, we synthesized and performed a detailed analysis of a novel low elastic modulus Ti-based alloy (Ti-28Nb-5Zr-2Ta-2Sn (TNZTS alloy)) using a variety of methods, including scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and tensile test. Additionally, the in vitro biocompatibility of the TNZTS alloy was evaluated using SCP-1, SaOs-2, and THP-1 cell lines and primary human osteoblasts. Compared to Ti-6Al-4V, the elastic modulus of TNZTS alloy was significantly lower, while measures of its in vitro biocompatibility are comparable. O2 plasma treatment of the surface of the alloy significantly increased its hydrophilicity and, hence, its in vitro biocompatibility. TNZTS alloy specimens did not induce the release of cytokines by macrophages, indicating that such scaffolds would not trigger inflammatory responses. The present results suggest that the TNZTS alloy may have potential as an alternative to Ti-6Al-4V.

scholarly journals Unraveling the complexity of amyloid polymorphism using gold nanoparticles and cryo-EM

2020 ◽  
Vol 117 (12) ◽  
pp. 6866-6874 ◽  
Author(s):  
Urszula Cendrowska ◽  
Paulo Jacob Silva ◽  
Nadine Ait-Bouziad ◽  
Marie Müller ◽  
Zekiye Pelin Guven ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gold Nanoparticles ◽  
Amyloid Fibrils ◽  
Imaging Techniques ◽  
Postmortem Brain ◽  
Structural Basis ◽  
Transmission Electron

Increasing evidence suggests that amyloid polymorphism gives rise to different strains of amyloids with distinct toxicities and pathology-spreading properties. Validating this hypothesis is challenging due to a lack of tools and methods that allow for the direct characterization of amyloid polymorphism in hydrated and complex biological samples. Here, we report on the development of 11-mercapto-1-undecanesulfonate-coated gold nanoparticles (NPs) that efficiently label the edges of synthetic, recombinant, and native amyloid fibrils derived from different amyloidogenic proteins. We demonstrate that these NPs represent powerful tools for assessing amyloid morphological polymorphism, using cryogenic transmission electron microscopy (cryo-EM). The NPs allowed for the visualization of morphological features that are not directly observed using standard imaging techniques, including transmission electron microscopy with use of the negative stain or cryo-EM imaging. The use of these NPs to label native paired helical filaments (PHFs) from the postmortem brain of a patient with Alzheimer’s disease, as well as amyloid fibrils extracted from the heart tissue of a patient suffering from systemic amyloid light-chain amyloidosis, revealed a high degree of homogeneity across the fibrils derived from human tissue in comparison with fibrils aggregated in vitro. These findings are consistent with, and strongly support, the emerging view that the physiologic milieu is a key determinant of amyloid fibril strains. Together, these advances should not only facilitate the profiling and characterization of amyloids for structural studies by cryo-EM, but also pave the way to elucidate the structural basis of amyloid strains and toxicity, and possibly the correlation between the pathological and clinical heterogeneity of amyloid diseases.

Mechanical, microstructural properties and cell adhesion of Sr/Se-hydroxyapatite/graphene/polycaprolactone nanofibers

2020 ◽  
pp. 089270572091278 ◽  
Author(s):  
Reem Al-Wafi ◽  
SF Mansour ◽  
MK Ahmed
Keyword(s):  
Electron Microscopy ◽  
Tensile Strength ◽  
Microstructural Properties ◽  
X Ray Diffraction ◽  
X Ray ◽  
Nanofibrous Scaffolds ◽  
Transmission Electron ◽  
Fesem Micrographs

Electrospun nanofibrous scaffolds containing co-dopant of Sr/Se into carbonated hydroxyapatite has been synthesized in situ with graphene (G) nanosheets and carried on polycaprolactone at different contributions of G. The powder and the nanofibrous samples were investigated using X-ray diffraction, transmission electron microscopy, and field emission scanning electron microscopy (FESEM). The FESEM micrographs show that the highest content of G (0.2 G) was formed in non-oriented/rough/cracked fibers with diameters around 0.3–0.4 µm at the maximum. The tensile strength of nanofibrous scaffolds was improved with the addition of G nanosheets and the maximum tensile strength of 0.2 G was around 6.39 ± 0.24 MPa, while the minimum cell viability ratio was about 94.4 ± 3.2% for the free G nanofibers. The in vitro attachment of HFB4 cell lines was investigated and it showed that nanofibrous scaffolds have induced cells to be proliferated and spread on the nanofibrous scaffolds’ surface. This behavior of cells growth encourages more investigations for these nanofibrous scaffolds to be promoted for clinical applications.

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